高效液相色谱法同时检测维生素功能饮料中16种成分
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摘要
建立高效液相色谱法同时检测维生素功能饮料中16种成分,包括12种水溶性维生素、2种甜味剂、1种色素及咖啡因含量的分析方法。取一定量饮料样品,超声脱气后,经0.45μm水系滤膜,经C18柱分离,二极管阵列检测器检测, 210 nm波长扫描,外标法定量。结果表明,高效液相色谱法同时检测维生素功能饮料中16种成分的方法检出限为0.1～0.5 mg/kg,当安赛蜜、咖啡因、阿斯巴甜的添加水平为5, 20和50 mg/kg,另外13种待测物的添加水平为2,5和10 mg/kg时,方法平均回收率为82.02%～105.62%, RSD为1.05%～5.23%(n=6)。该方法操作简便快速、准确、重现性好,适用于同时检测维生素功能饮料中16种成分的含量。
A method for the simultaneous determination of 16 kinds of ingredients including 12 kinds of water-soluble vitamins, 2 kinds of sweeteners, a kind of colouring and caffeine in vitamins functional beverages by high performance liquid chromatography(HPLC) was established. The samples were injected in HPLC after ultrasonic treatment, filtrating with 0.45 μm micropore filters, separated by C18 column, and determined by photo-diode array at 210 nm wavelength with external standard method for quantification. Result showed that the limits of the detection(LOD) of the 16 kinds of fluorescent whitening agent which were detected by high performance liquid chromatography in vitamins functional beverages were 0.1-0.5 mg/kg. The recoveries of the 16 kinds of fluorescent whitening agent were between 82.02% and 105.62% when acesulfame, caffeine and aspartame were spiked at 5, 20 and 50 mg/kg and spiked at 2, 5 and 10 mg/kg in the other 13 kinds of objects to be tested. The relative standard deviations of the method were less than 5.23%(n=6). This method was simple, rapid, accurate, and repeatable, which could be used for simultaneous analysis of 16 kinds of ingredients in vitamins functional beverages.
A method for the simultaneous determination of 16 kinds of ingredients including 12 kinds of water-soluble vitamins, 2 kinds of sweeteners, a kind of colouring and caffeine in vitamins functional beverages by high performance liquid chromatography(HPLC) was established. The samples were injected in HPLC after ultrasonic treatment, filtrating with 0.45 μm micropore filters, separated by C18 column, and determined by photo-diode array at 210 nm wavelength with external standard method for quantification. Result showed that the limits of the detection(LOD) of the 16 kinds of fluorescent whitening agent which were detected by high performance liquid chromatography in vitamins functional beverages were 0.1-0.5 mg/kg. The recoveries of the 16 kinds of fluorescent whitening agent were between 82.02% and 105.62% when acesulfame, caffeine and aspartame were spiked at 5, 20 and 50 mg/kg and spiked at 2, 5 and 10 mg/kg in the other 13 kinds of objects to be tested. The relative standard deviations of the method were less than 5.23%(n=6). This method was simple, rapid, accurate, and repeatable, which could be used for simultaneous analysis of 16 kinds of ingredients in vitamins functional beverages.
引文
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[27]王荣艳,贾丽,钱春燕. HPLC-PDA法同时测定功能性饮料中9种水溶性维生素[J].现代科学仪器, 2010(4):98-101.
[28]忻欣,高飞,韩莹,等.高效液相色谱法测定饮料中B族维生素[J].食品研究与开发, 2018(2):173-176.
[29]李强,李挥,庞坤,等.功能性饮料中11种水溶性维生素的高通量检测技术研究[J].河北省科学院学报, 2012,29(3):44-48.
[30]徐烨,顾鑫荣,王瑞菲,等.反相高效液相色谱法同时测定功能饮料中六种B族维生素[J].理化检验(化学分册),2010, 46(5):497-499.
[2] PAWLAK R. Is vitamin B12 deficiency a risk factor for cardiovascular disease in vegetarians?[J]. American Journal of Preventive Medicine, 2015, 48(6):e11.
[3]MITCHELLES,CONUSN,KAPUTJ.Bvitamin polymorphisms and behavior:evidence of associations with neurodevelopment,depression,schizophrenia,bipolar disorder and cognitive decline[J]. Neurosci Biobehav Rev,2014, 47(14):307-320.
[4] MIKKELSEN K, STOJANOVSKA L, PRAKASH M, et al.The effects of vitamin B on the immune/cytokine network and their involvement in depression[J]. Maturitas, 2017, 96:58-71.
[5] STRUIJK E A, LANA A, GUALLARCASTILLóN P, et al.Intake of B vitamins and impairment in physical function in older adults[J]. Clinical Nutrition, 2017, 37(4):1271-1278.
[6] ELTONY S A. Histological study on the protective role of vitamin B Complex on the cerebellum of diabetic rat[J].Tissue&Cell, 2016, 48(4):283-296.
[7] STROHM D, BECHTHOLD A, ISIK N, et al. Revised reference values for the intake of thiamin(vitamin B1),riboflavin(vitamin B2), and niacin[J]. Nfs Journal, 2016, 3(C):20-24.
[8]中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中维生素B1的测定:GB 5009.84—2016[S].北京:中国标准出版社, 2016.
[9]中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准食品中维生素B2的测定:GB 5009.85—2016[S].北京:中国标准出版社,2016.
[10]中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准食品中维生素B6的测定:GB 5009.154—2016[S].北京:中国标准出版社,2016.
[11]中华人民共和国卫生部.食品安全国家标准婴幼儿食品和乳品中维生素B12的测定:GB 5413.14—2010[S].北京:中国标准出版社, 2010.
[12]中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准食品中烟酸和烟酰胺的测定:GB 5009.89—2016[S].北京:中国标准出版社, 2016.
[13]中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准食品中阿斯巴甜和阿力甜的测定:GB 5009.263—2016[S].北京:中国标准出版社, 2016.
[14]中华人民共和国卫生部,中国国家标准化管理委员会.饮料中乙酰磺胺酸钾的测定:GB/T 5009.140—2003[S].北京:中国标准出版社, 2003.
[15]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.水果罐头中合成着色剂的测定高效液相色谱法:GB/T 21916—2008[S].北京:中国标准出版社, 2008.
[16]中华人民共和国国家卫生和计划生育委员会.食品安全国家标准饮料中咖啡因的测定:GB 5009.139—2014[S].北京:中国标准出版社, 2014.
[17]成志强,孙成均,黎源倩.反相高效液相色谱法同时测定食品和多维片中8种水溶性维生素[J].分析化学, 2001,29(9):1068-1071.
[18]吴淑君,庄志辉,朱孟丽,等.高效液相色谱法测定保健品中的6种水溶性维生素及咖啡因[J].色谱, 2006, 24(3):319-319.
[19]李克,王华娟,潘朝晖,等.离子对反相高效液相色谱法同时测定复合维生素片中4种水溶性维生素[J].色谱,2003, 21(1):66-68.
[20]高旭,李淑娟,安娟,等.高效液相色谱法同时测定食品中8种水溶性维生素[J].中国医药指南, 2008, 6(19):36-38.
[21]梁瑞强,王聪,曹进,等.高效液相色谱-串联质谱法测定保健食品中10种水溶性维生素[J].食品安全质量检测学报, 2016, 7(3):966-974.
[22]朱晓玲,张苗,王会霞,等.功能饮料、保健食品中11种水溶性维生素测定的HPLC-MS/MS方法研究[J].食品科技, 2017(7):294-300.
[23]沙如意,吴迪,蔡成岗,等.高效液相色谱法测定饮料中的功能性成分[J].食品工业, 2017, 38(3):299-301.
[24]杨玲娟,谢天柱,赵素瑞.离子对反相高效液相色谱法同时测定功能性饮料中的B族维生素[J].中国酿造, 2009,28(8):140-142.
[25]张志清,白琦,湛珺雯,等. RP-HPLC法测定功能性饮料水溶性维生素含量[J].食品科学, 2010, 31(14):212-215.
[26]王一红,冯家力,潘振球,等.液相色谱-串联质谱法分析10种水溶性维生素[J].中国卫生检验杂志, 2007, 17(7):1160-1162.
[27]王荣艳,贾丽,钱春燕. HPLC-PDA法同时测定功能性饮料中9种水溶性维生素[J].现代科学仪器, 2010(4):98-101.
[28]忻欣,高飞,韩莹,等.高效液相色谱法测定饮料中B族维生素[J].食品研究与开发, 2018(2):173-176.
[29]李强,李挥,庞坤,等.功能性饮料中11种水溶性维生素的高通量检测技术研究[J].河北省科学院学报, 2012,29(3):44-48.
[30]徐烨,顾鑫荣,王瑞菲,等.反相高效液相色谱法同时测定功能饮料中六种B族维生素[J].理化检验(化学分册),2010, 46(5):497-499.