具有荧光、磁性的纳米抗菌性复合材料的制备及回收利用
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摘要
采用聚甲基丙烯酸缩水甘油酯(PGMA)作为模板成功制备了新型具有磁性-抗菌性-荧光性的多功能复合微球。合成的PGMA、氨基化的PGMA(NH2-PGMA)、磁性PGMA(M-PGMA)都呈表面光滑的球形,并且具有良好的单分散性。M-PGMA具有超顺磁性,使微球在外加磁场的作用下可以从溶液中快速分离出来。引入聚六亚甲基胍酸盐(PHGH)复合微球具有显著的抗菌效果。荧光物质碲化镉(CdTe)通过静电吸附作用吸附在球体表面使微球具有了荧光性能,这就使得复合微球具有靶向定位、生物检测等功能。将磁性,抗菌和荧光结合到一个单一的复合微球中,开辟了对多功能材料进行广泛研究的可能性,并拓宽了潜在的应用范围。
A novel multi-functional composite microsphere with magnetic-antibacterial-fluorescence was successfully prepared by using polyglycidyl methacrylate(PGMA)as a template.The as-synthesized microspheres PGMA,amino-modified PGMA(NH2-PGMA),and magnetic PGMA(M-PGMA)all had smooth spherical surfaces and fine monodispersity.M-PGMA microspheres had superparamagnetism,which made M-PGMA to be effectively separated from the solution by an external magnetic field.The introduction of poly(haxemethylenguanidine hydrochloride)(PHGH)made the hybrid microspheres have significant antibacterial effect.Fluorescent features(CdTe)adsorbed on the microspheres surface by electrostatic adsorption imparted biological functions to the hybrid microspheres.The combination of magnetic,antibacterial and fluorescent light into a single hybrid microsphere opens up the possibility of extensive research on multifunctional materials and broadens the range of potential applications.
A novel multi-functional composite microsphere with magnetic-antibacterial-fluorescence was successfully prepared by using polyglycidyl methacrylate(PGMA)as a template.The as-synthesized microspheres PGMA,amino-modified PGMA(NH2-PGMA),and magnetic PGMA(M-PGMA)all had smooth spherical surfaces and fine monodispersity.M-PGMA microspheres had superparamagnetism,which made M-PGMA to be effectively separated from the solution by an external magnetic field.The introduction of poly(haxemethylenguanidine hydrochloride)(PHGH)made the hybrid microspheres have significant antibacterial effect.Fluorescent features(CdTe)adsorbed on the microspheres surface by electrostatic adsorption imparted biological functions to the hybrid microspheres.The combination of magnetic,antibacterial and fluorescent light into a single hybrid microsphere opens up the possibility of extensive research on multifunctional materials and broadens the range of potential applications.
引文
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[15] Zhou Huajian,Cao Lixin,Gao Rongjie,et al.Preparation and application of water-soluble CdTe quantum dot fluorescent probes[J].Chinese Journal of Luminescence,2013,34(07):829-835(in Chinese).周华健,曹立新,高荣杰,等.水溶性CdTe量子点荧光探针的制备表征及应用[J].发光学报,2013,34(07):829-835.
[2] Guan Y,Xian H N,Sullivan H,et al.Antimicrobialmodified sulfite pulps prepared by in situ copolymerization[J].Carbohydr Polym,2007,69(4):688-696.
[3] Peng Kaimei,Ding Wei,Tu Weiping.Construction and application of antibacterial polymers for terpenoids[J].Acta Chimica Sinica,2016,74(09):713-725(in Chinese).彭开美,丁伟,涂伟萍.胍类抗菌聚合物的构建及应用[J].化学学报,2016,74(09):713-725.
[4] Zeng Xiuni.Analysis of Chinese Patent(CN)for preparation and application of polymer/nano-SiO2composites[J].Plastic Additives,2014(03):5-11(in Chinese).曾秀妮.聚合物/纳米二氧化硅复合材料的制备、应用的有关中国专利(CN)分析[J].塑料助剂,2014(03):5-11.
[5] Qian L Y,Guan Y,He B H,et al.Modified guanidine polymers:synthesis and antimicrobial mechanism revealed by AFM[J].Polymer,2008,49(10):2471-2475.
[6] Shukoor M I,Natalio F,Tahir M N,et al.Superparamagneticγ-Fe2O3nanoparticles with tailored functionality for protein separation[J].Chem Commun,2007,(44):4677-4679.
[7] Geng Yuwei,Hu Zhixiong,Zhang Weinong,et al.Synthesis and characterization of magnetic polyaminomethaneboronic acid nanoparticles[J].Journal of Wuhan University of Light Industry,2014,33(01):45-48(in Chinese).晏雨微,胡志雄,张维农,等.磁性聚间氨基苯硼酸纳米粒子的合成及表征[J].武汉轻工大学学报,2014,33(01):45-48.
[8] Kattnig D R,Rosspeintner A,Grampp G.Fully reversible interconversion between locally excited fluorophore,exciplex,and radical ion pair demonstrated by a new magnetic field effect[J].Angew Chem Int Ed,2008,47(5):960-962.
[9] Wang P,Shi Q,Shi Y,et al.Magnetic permanently confined micelle arrays for treating hydrophobic organic compound contamination[J].Journal of the American Chemical Society,2009,131(1):182-188.
[10] Dai Ming,Liao Yuanbao,Liu Dong,et al.Growth and photoluminescence properties of CdTe nanocrystals and layered nanocrystalline films[J].Acta Physica Sinica,2009,58(10):7246-7249(in Chinese).戴明,廖远宝,刘东,等.CdTe纳米晶及层状纳米晶薄膜的生长及其光荧光性质[J].物理学报,2009,58(10):7246-7249.
[11] Sun P,Zhang H Y,Liu C,et al.Preparation and characterization of Fe3O4/CdTe magnetic/fluorescent nanocomposites and their applications in immuno-labeling and fluorescent imaging of cancer cells[J].Langmuir,2010,26(2):1278-1284.
[12] Dong aili del tu.Preparation and properties of antibacterial nanocomposites based on N-halamines[D].Changchun:Jilin University,2012(in Chinese).董阿力德尔图.基于N-卤胺的抗菌纳米复合材料的制备及性能研究[D].长春:吉林大学,2012.
[13] Mo Zunli,Hu Jay,Wang Yawen,et al.Antibacterial materials and their antibacterial mechanism[J].Materials Review,2014,28(01):50-52(in Chinese).莫尊理,胡惹惹,王雅雯,等.抗菌材料及其抗菌机理[J].材料导报,2014,28(01):50-52.
[14] Guo J,Yang W L,Wang C C,et al.Poly(N-isopropylacrylamide)-coated luminescent/magnetic silica microspheres:preparation,characterization,and biomedical applications[J].Chemistry of Materials,2006,18(23):5554-5562.
[15] Zhou Huajian,Cao Lixin,Gao Rongjie,et al.Preparation and application of water-soluble CdTe quantum dot fluorescent probes[J].Chinese Journal of Luminescence,2013,34(07):829-835(in Chinese).周华健,曹立新,高荣杰,等.水溶性CdTe量子点荧光探针的制备表征及应用[J].发光学报,2013,34(07):829-835.
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