脑瘫动物模型研究进展
|
摘要
脑性瘫痪的发病原因与机制复杂、多样,建立稳定的动物模型是后续研究的基础。目前脑瘫模型制备方法主要有缺血缺氧型、感染型、神经毒素型、脑外伤型、多法联合型5种。在诸多的脑瘫模型中,大鼠单侧颈总动脉结扎合并缺氧环境动物模型被认为最为成熟,成功率高,易于操作,重复性好,实验结果稳定可靠,运用较为广泛。感染模型中,应用孕鼠腹腔注射脂多糖(LPS)致宫内感染合并缺氧的方法更为成熟,具有创伤小,简单易行,能够逼真模拟人类在怀孕晚期宫内感染缺氧致胎儿脑部损伤等优点。
The mechanism of cerebral palsy is complicated and varied, and making stable animal models is the basis of follow-up study. At present, there are mainly five ways to make animal models of cerebral palsy, which are hypoxia-ischemia, infection, neurotoxin, cerebral trauma and the multi-method combination. Among many models of cerebral palsy, it is considered that the most mature one is the animal model made with rats of unilaterally common carotid artery ligature combined with anoxic environment, with a higher success rate, easy operation, good reproducibility, stable and reliable experimental results. Therefore, it is widely used as animal models of cerebral palsy. Among infection models, it is considered that the more mature method is to adopt the intrauterine infection of pregnant rats induced by intraperitoneal injection with lipopolysaccharide(LPS) combined with anoxic environment, with less trauma and easy operability. Moreover, this method can vividly simulate fetal cerebral damage caused by intrauterine infection and anoxia of pregnant women in the late stage.
The mechanism of cerebral palsy is complicated and varied, and making stable animal models is the basis of follow-up study. At present, there are mainly five ways to make animal models of cerebral palsy, which are hypoxia-ischemia, infection, neurotoxin, cerebral trauma and the multi-method combination. Among many models of cerebral palsy, it is considered that the most mature one is the animal model made with rats of unilaterally common carotid artery ligature combined with anoxic environment, with a higher success rate, easy operation, good reproducibility, stable and reliable experimental results. Therefore, it is widely used as animal models of cerebral palsy. Among infection models, it is considered that the more mature method is to adopt the intrauterine infection of pregnant rats induced by intraperitoneal injection with lipopolysaccharide(LPS) combined with anoxic environment, with less trauma and easy operability. Moreover, this method can vividly simulate fetal cerebral damage caused by intrauterine infection and anoxia of pregnant women in the late stage.
引文
[1]李静,樊祥伟,高晶,等.电针刺激头部运动区对脑瘫鼠大脑磁共振弥散张量纤维束成像的影响[J].中医药信息,2015,32(6):63.
[2]TAI W C,BURKE K A,DOMINGUEZ J F,et al.Growth deficits in a postnatal day 3 rat model of hypoxic-ischemic brain injury[J].Behavioural Brain Research,2009,202(1):40-49.
[3]陈刚,王伟哲,陈明钊,等.新生幼鼠缺血缺氧性脑损伤模型的实验研究[J].中国康复理论与实践,2012,18(7):612-614.
[4]VEHARA H,YOSHIOKA H,KAWASE S,et al.A new model of white matter injury in neonatal rats with bilateral carotid artery occlusion[J].Brain Res,1999,837(1-2):213-220.
[5]王江涛,毛英丽,李洪华,等.新生大鼠痉挛型脑瘫动物模型构建及评价的实验研究[J].中国妇幼保健,2011,19(26):2992-2995.
[6]SHENANDOAH R,KASIA P,QING L,et al.Developmental changes induced by graded prenatal systemic hypoxicischemic insults in rats[J].Neurobiology of Disease,2005(18):568-581.
[7]李晓捷,高晶,孙忠人,等.宫内感染致早产鼠脑瘫动物模型制备及其鉴定的实验研究[J].中国康复医学杂志,2004(12):885-889.
[8]陈刚,胡燕荣,钟杰,等.孕鼠腹腔注射脂多糖合并缺氧环境造成宫内胎鼠脑部损伤[J].中国康复理论与实践,2009,9(15):807-809.
[9]TERRONE D,RINEHART B K,GRANGER J P,et al.Interleukin-10 administration and bacterial endotoxin-induced preterm birth in a rat model[J].Obstetrics&Gynecology,2001,98(3):476-480.
[10]POGGI S,JANE P,LAURA T,et al.No phenotype associated with established lipopolysaccharide model for cerebral palsy[J].American Journal of Obstetrics and Gynecology,2005(192):727-733.
[11]SAADANIMAKKI F,KANNAN S,LU X,et al.Intrauterine administra-tion of endotoxin leads to motor deficits in a rabbit model:a linkbetween prenatal infection and cerebral palsy[J].Am J Obstet Gy-necol,2008,199(6):651-657.
[12]孙叶强,李晓捷,吴军,等.胆红素致新生兔脑瘫动物模型的实验研究[J].中国康复,1999,14(2):65-67.
[13]李晓捷,吴军,孙叶强,等.兔高胆红素血症性脑性瘫痪的病理改变研究.现代康复,2001,5(6):70-75.
[14]EVELINV,MATHEUSB,MARINAL,etal..Cerebrospinalfluid S100B increases reversibly in neonates of methyl mercury-intoxicated pregnant rats[J].Neuro T ox icology,2004(25):771-777.
[15]WANG T,ZHANG L,JIANG L,et al.Neurotox icological effects of 3-nitropropionic acid on the neonatal rat[J].Neurotoxicology,2008,29(6):1023-1029.
[16]DIXON C E,LVETH B G,POVLISHOCK J K,A fluid percussion model ofex perimental brain injury in the rat[J].JNeurosurg,1987(67):110-119.
[17]FEENEY D M,BOY ESON M G,LINN R T,et al.Responses to corticalinjury:M ethodology and local effects of contusions in the rat[J].Brain Res,1981(211):67-77.
[18]张敬东,张新,俞兴.构建大鼠痉挛型脑性瘫痪模型[J].中国临床康复,2006,10(38):150-151.
[19]俞雅珍,邓亚仙,高宝勤,等.电损毁致大鼠痉挛性脑性瘫痪动物模型制备及其鉴定[J].实用儿科临床杂志,2007,22(12):928-929.
[20]AUDREY B C,JOHANNES M,YVES G,et al.Intracisternal application of endotoxin enhances thesusceptibility to subse-quent hypoxic-ischemic brain damage in neonatal rats[J].Pediatric Re-serch,2003(53):770-775.
[21]GIRARD S,KADHIM H,BEAUDET N,et al.Developmental motor deficits in-duced by combined fetal exposure to lipopolysaccharide and early neo-natal hypoxia/ischemia:a novel animal model for cerebral palsy in verypremature infants[J].Neuroscience,2009(158):673-682.
[22]DROBYSHEVSKY A,DERRICK M,WYRWICZ A M,et al.White matterinjury correlates with hypertonia in an animal model of cerebraIpalsy[J].J Cereb Blood Flow Metab,2007,27(2):270-281.
[2]TAI W C,BURKE K A,DOMINGUEZ J F,et al.Growth deficits in a postnatal day 3 rat model of hypoxic-ischemic brain injury[J].Behavioural Brain Research,2009,202(1):40-49.
[3]陈刚,王伟哲,陈明钊,等.新生幼鼠缺血缺氧性脑损伤模型的实验研究[J].中国康复理论与实践,2012,18(7):612-614.
[4]VEHARA H,YOSHIOKA H,KAWASE S,et al.A new model of white matter injury in neonatal rats with bilateral carotid artery occlusion[J].Brain Res,1999,837(1-2):213-220.
[5]王江涛,毛英丽,李洪华,等.新生大鼠痉挛型脑瘫动物模型构建及评价的实验研究[J].中国妇幼保健,2011,19(26):2992-2995.
[6]SHENANDOAH R,KASIA P,QING L,et al.Developmental changes induced by graded prenatal systemic hypoxicischemic insults in rats[J].Neurobiology of Disease,2005(18):568-581.
[7]李晓捷,高晶,孙忠人,等.宫内感染致早产鼠脑瘫动物模型制备及其鉴定的实验研究[J].中国康复医学杂志,2004(12):885-889.
[8]陈刚,胡燕荣,钟杰,等.孕鼠腹腔注射脂多糖合并缺氧环境造成宫内胎鼠脑部损伤[J].中国康复理论与实践,2009,9(15):807-809.
[9]TERRONE D,RINEHART B K,GRANGER J P,et al.Interleukin-10 administration and bacterial endotoxin-induced preterm birth in a rat model[J].Obstetrics&Gynecology,2001,98(3):476-480.
[10]POGGI S,JANE P,LAURA T,et al.No phenotype associated with established lipopolysaccharide model for cerebral palsy[J].American Journal of Obstetrics and Gynecology,2005(192):727-733.
[11]SAADANIMAKKI F,KANNAN S,LU X,et al.Intrauterine administra-tion of endotoxin leads to motor deficits in a rabbit model:a linkbetween prenatal infection and cerebral palsy[J].Am J Obstet Gy-necol,2008,199(6):651-657.
[12]孙叶强,李晓捷,吴军,等.胆红素致新生兔脑瘫动物模型的实验研究[J].中国康复,1999,14(2):65-67.
[13]李晓捷,吴军,孙叶强,等.兔高胆红素血症性脑性瘫痪的病理改变研究.现代康复,2001,5(6):70-75.
[14]EVELINV,MATHEUSB,MARINAL,etal..Cerebrospinalfluid S100B increases reversibly in neonates of methyl mercury-intoxicated pregnant rats[J].Neuro T ox icology,2004(25):771-777.
[15]WANG T,ZHANG L,JIANG L,et al.Neurotox icological effects of 3-nitropropionic acid on the neonatal rat[J].Neurotoxicology,2008,29(6):1023-1029.
[16]DIXON C E,LVETH B G,POVLISHOCK J K,A fluid percussion model ofex perimental brain injury in the rat[J].JNeurosurg,1987(67):110-119.
[17]FEENEY D M,BOY ESON M G,LINN R T,et al.Responses to corticalinjury:M ethodology and local effects of contusions in the rat[J].Brain Res,1981(211):67-77.
[18]张敬东,张新,俞兴.构建大鼠痉挛型脑性瘫痪模型[J].中国临床康复,2006,10(38):150-151.
[19]俞雅珍,邓亚仙,高宝勤,等.电损毁致大鼠痉挛性脑性瘫痪动物模型制备及其鉴定[J].实用儿科临床杂志,2007,22(12):928-929.
[20]AUDREY B C,JOHANNES M,YVES G,et al.Intracisternal application of endotoxin enhances thesusceptibility to subse-quent hypoxic-ischemic brain damage in neonatal rats[J].Pediatric Re-serch,2003(53):770-775.
[21]GIRARD S,KADHIM H,BEAUDET N,et al.Developmental motor deficits in-duced by combined fetal exposure to lipopolysaccharide and early neo-natal hypoxia/ischemia:a novel animal model for cerebral palsy in verypremature infants[J].Neuroscience,2009(158):673-682.
[22]DROBYSHEVSKY A,DERRICK M,WYRWICZ A M,et al.White matterinjury correlates with hypertonia in an animal model of cerebraIpalsy[J].J Cereb Blood Flow Metab,2007,27(2):270-281.