一株马铃薯干腐病病原菌的鉴定与药剂筛选
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摘要
分离了一株马铃薯干腐病病原菌,经ITS序列分析证实其为尖刀镰孢菌(Fusarium oxysporum),通过培养后喷雾接种感病品种AC0338,接种3 d后采用5种药剂进行防治。结果表明,5种药剂处理对马铃薯块茎干腐病均有一定的防治效果,其中以40%多菌灵悬浮剂500倍液处理防效最好,防治效果为72.49%,能有效地控制马铃薯干腐病菌的扩展和蔓延。
A strain of Fusarium oxysporum isolated from potato dry rot was identified by inter translated spacer sequencing. 5kinds of fungicides for prevention and control dry rot were tested after the strain of dry rot pathogen was isolated in potato tuber and inoculated on potato cultivar of AC0338 for three days. The results showed that all the fungicides had certain effects. Control effect of 40% of carbendazim suspending agent of 500 times fungicide being of 72.49%, which was higher than others. It suggested that carbendazim could effectively control the potato dry rot spreading.
A strain of Fusarium oxysporum isolated from potato dry rot was identified by inter translated spacer sequencing. 5kinds of fungicides for prevention and control dry rot were tested after the strain of dry rot pathogen was isolated in potato tuber and inoculated on potato cultivar of AC0338 for three days. The results showed that all the fungicides had certain effects. Control effect of 40% of carbendazim suspending agent of 500 times fungicide being of 72.49%, which was higher than others. It suggested that carbendazim could effectively control the potato dry rot spreading.
引文
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[3]张廷义,魏周全.马铃薯贮藏期块茎干腐病药剂防治试验[J].中国马铃薯,2006,20(6):348-349.
[4]张杰,张廷义.定西市马铃薯贮藏期主要病害及成因分析[J].中国马铃薯,2008,22(1):55-56.
[5]彭学文,朱杰华.河北省马铃薯真菌病害种类及分布[J].中国马铃薯,2008,22(1):31-33.
[6]叶琪明,王拱辰.浙江马铃薯干腐病病原研究初报[J].植物病理学报,1994,25(2):148.
[7]何苏琴,金秀琳,魏周全,等.甘肃省定西地区马铃薯块茎干腐病病原真菌的分离鉴定[J].云南农业大学学报,2004,19(5):550-552.
[8]魏巍.河北和内蒙古马铃薯干腐病菌种类鉴定及优势病原菌的分子检测[D].河北保定:河北农业大学,2013.
[9]闵凡祥,王晓丹,胡林双,等.黑龙江省马铃薯干腐病菌种类鉴定及致病性[J].植物保护,2010,36(4):112-115.
[10]薛玉凤.马铃薯枯萎病病原菌学初步研究[D].呼和浩特:内蒙古农业大学,2012.