高效液相色谱法检测水中的氟噻草胺
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摘要
[目的]建立高效液相色谱法检测水中的氟噻草胺。[方法]水样经乙腈提取,以乙腈-0.1%甲酸水(体积比70∶30)为流动相,Agilent TC-C_(18)(2)色谱柱分离,在225 nm检测波长下,对氟噻草胺进行定量测定。[结果]在1～100mg/L范围内,氟噻草胺的质量浓度与对应的峰面积间呈良好的线性关系;在添加水平为5、50 mg/L时,氟噻草胺在水中的平均回收率为89.4%～103.1%,相对标准偏差(RSD)分别为4.7%和1.2%。[结论]该方法操作简便、快捷,线性关系良好,准确度、精密度符合农药定量分析要求,可用于水中氟噻草胺的定量分析检测。
[Aims] An analysis method using high performance liquid chromatography(HPLC) was developed for the determination of flufenacet in water. [Methods] The water samples were extracted with acetonitrile, followed by quantitative detection by HPLC using Agilent TC-C_(18)(2) column, and with the mixture solution of acetonitrile and 0.1%formic acid water(70∶30, by vol) as mobile phase at 225 nm. [Results] There was a good linear relationship between the target substance concentration and the peak area in the range of 1-100 mg/L. Besides, the average recoveries were89.4-103.1% at the spiked levels of 5 and 50 mg/L, and the relative standard deviations(RSD) were 4.7 and 1.2%,respectively. [Conclusions] The analysis method is simple and convenient, and the accuracy and precision meet with the requirement for pesticide quantitative analysis, which can be applied for determination of flufenacet in water.
[Aims] An analysis method using high performance liquid chromatography(HPLC) was developed for the determination of flufenacet in water. [Methods] The water samples were extracted with acetonitrile, followed by quantitative detection by HPLC using Agilent TC-C_(18)(2) column, and with the mixture solution of acetonitrile and 0.1%formic acid water(70∶30, by vol) as mobile phase at 225 nm. [Results] There was a good linear relationship between the target substance concentration and the peak area in the range of 1-100 mg/L. Besides, the average recoveries were89.4-103.1% at the spiked levels of 5 and 50 mg/L, and the relative standard deviations(RSD) were 4.7 and 1.2%,respectively. [Conclusions] The analysis method is simple and convenient, and the accuracy and precision meet with the requirement for pesticide quantitative analysis, which can be applied for determination of flufenacet in water.
引文
[1]刘长令.世界农药大全:除草剂卷[M].北京:化学工业出版社2002:251.
[2]姜育田,陈同明,李茂青.氟噻草胺的合成[J].农药,2007,46(11)734-736.
[3]高兴祥,王宝亮,房锋,等.氟噻草胺生物活性及对玉米和大豆安全性室内测定[J].植物保护学报,2017,44(4):703-704.
[4]范志业,沈海龙,陈琦,等.吡氟·氟噻·呋草酮悬浮剂土壤封闭和茎叶处理对冬小麦田阔叶杂草的防除效果比较[J].农药,201756(1):65-68.
[5]吴仁海,孙慧慧,苏旺苍,等.氟噻草胺与二甲戊灵混配对多花黑麦草的协同控制作用及对小麦的安全性[J].河南农业科学,2017,46(6):89-92.
[6]SAKKAS V A,CALZA P,VLACHOU A D,et al.Photocatalytic Transformation of Flufenacet over TiO2Aqueous Suspensions:Identification of Intermediates and the Mechanism Involved[J].Applied Catalysis B:Environmental,2011,110(41):238-250.
[2]姜育田,陈同明,李茂青.氟噻草胺的合成[J].农药,2007,46(11)734-736.
[3]高兴祥,王宝亮,房锋,等.氟噻草胺生物活性及对玉米和大豆安全性室内测定[J].植物保护学报,2017,44(4):703-704.
[4]范志业,沈海龙,陈琦,等.吡氟·氟噻·呋草酮悬浮剂土壤封闭和茎叶处理对冬小麦田阔叶杂草的防除效果比较[J].农药,201756(1):65-68.
[5]吴仁海,孙慧慧,苏旺苍,等.氟噻草胺与二甲戊灵混配对多花黑麦草的协同控制作用及对小麦的安全性[J].河南农业科学,2017,46(6):89-92.
[6]SAKKAS V A,CALZA P,VLACHOU A D,et al.Photocatalytic Transformation of Flufenacet over TiO2Aqueous Suspensions:Identification of Intermediates and the Mechanism Involved[J].Applied Catalysis B:Environmental,2011,110(41):238-250.