中高温α-淀粉酶热稳定性的研究进展
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摘要
指出了α-淀粉酶的耐热性已成为当今研究的热点。阐述了对高热稳定性α-淀粉酶的研究意义,总结了α-淀粉酶的来源并主要列举了微生物来源的中高温α-淀粉酶,结合该领域的相关研究对α-淀粉酶热稳定性的影响因素及改造方法进行了综述,为完善α-淀粉酶的热稳定化技术提供参考依据。
The heat resistance of alpha-amylase has become a hot research topic.This paper expounds the research significance of high thermal stability of alpha-amylase;summarized the source of alpha-amylase and lists some main microbial source of high temperature of alpha-amylase.It reviewedfactors influencing on the thermal stability of alpha-amylase and improved methodcombined with the related research in the field,providing a reference for improving the thermal stabilization technology of alpha-amylase.
The heat resistance of alpha-amylase has become a hot research topic.This paper expounds the research significance of high thermal stability of alpha-amylase;summarized the source of alpha-amylase and lists some main microbial source of high temperature of alpha-amylase.It reviewedfactors influencing on the thermal stability of alpha-amylase and improved methodcombined with the related research in the field,providing a reference for improving the thermal stabilization technology of alpha-amylase.
引文
[1]张强.耐高温α-淀粉酶产生菌的分离鉴定及发酵条件与酶学性质研究[D].成都:四川大学,2005.
[2]王勇,王行,贺广生,等.耐高温产淀粉酶芽孢杆菌在烟叶烘烤中降解淀粉的应用研究[J].中国烟草学报,2017,23(4):56～63.
[3]李习.高产α-淀粉酶地衣芽孢杆菌的筛选及重组质粒的构建[D].武汉:湖北工业大学,2014.
[4]岳寿松,边斐,代运章,等.产蛋白酶和淀粉酶芽孢杆菌SDYB-1的分子鉴定及酶学性能研究[J].山东农业科学,2015,47(11):54～59.
[5]MEHTA D,SATYANARAYANA T.Biochemical and molec-ular characterization of recombinant acidic and thermostable raw-starch hydrolysing α-amylase from an extreme thermophileGeobacillus thermoleovorans[J].Journal of Molecular CatalysisB:Enzymatic,2013(85-86):229～238.
[6]EMTENANI S,ASOODEH A,EMTENANI S.Gene cloningand characterization of a thermostable organic-tlerant α-amylase from Bacillus subtilis DR8806 [J].InternationalJournal of Biological Macromolecules,2015(72):290～298.
[7]SIDDIQUE F,HUSSAIN I,MAHMOOD M S,et al.Isolationand characterization of a highly thermostable alha-amylase enzyme produced by Bacillus licheniforms[J].Pakistan Journal ofAgricultural Sciences,2014,51(2):309～314.
[8]WU Xiang-rong,WANG Yu-xia,TONG Ben-ding,et al.Purifi-cation and biochemical characterization of a themostable andacid-stable alpha-amylase fromBacillus lichenifomis B4-423[J].In-ternational Journal of Biological Macromolecules,2017(109):329～337.
[9]JANA M,MAITY C,SAMANTA S,et al.Salt-independentthermophilicα-amylase from Bacillus megaterium VMB109:An efficacy testing for preparation of maltooligsaccharides[J].Industrial Crops & Products,2013,41(1):386～391.
[10]EMTENANI S,ASOODEH A,EMTENANI S.Gene cloningand characterization of a thermostable organic-tlerant α-amylase from Bacillus subtilis DR8806 [J].InternationalJournal of Biological Macromolecules,2015(72):290～298.
[11]李松,王正祥.真菌α-淀粉酶的研究进展[J].生物技术通报,2011(3):66～71.
[12]AQUINO A,JORGE J A,TERENZI H F,et al.Studies on athermostable alpha-amylase from the thermophilic fungusScytalidium thermophilum[J].Applied Microbiology and Bio-technology,2003,61(4):323～328.
[13]ALI I,AKBAR A,ANWAR M,et al.Purification and charac-terization of a polyextremophilicα-amylase from nobligate hal-ophilic aspergillus penicillioides isolate and is potential for souse with detergents[J].Biomed Research International,2015(10).
[14]朱何东,陈远钊,常峰,等.高温α-淀粉酶产生菌的筛选及酶学性质研究[J].酿酒科技,2006(5):30～32.
[15]杨自朋,张金辉,苏静,等.一株黑曲霉所产α-淀粉酶的提取及其特性研究[J].河南职工医学院学报,2006(6):431～433.
[16]田方源,汤斌,李松,等.米根霉α-淀粉酶高麦芽糖生成能力相关氨基酸的初步分析[J].食品与发酵工业,2017,43(8):27～32.
[17]何胜华,罗聪,李海梅,等.米曲霉KFRI 888产中性蛋白酶、a-淀粉酶酶学性质的研究[J].食品工业科技,2006(9):49～50+53.
[18]罗文杰.耐热酸性淀粉酶筛选及酶学特性研究[D].杭州:浙江工业大学,2008.
[19]张强,刘成君,蒋芳,等.耐高温α-淀粉酶产生菌的分离鉴定及发酵条件与酶性质研究[J].食品与发酵工业,2005(2):34～37.
[20]李祝.嗜热脂肪芽孢杆菌α-淀粉酶的异源表达及热稳定性改造[D].无锡:江南大学,2018.
[21]赵荧,费小芳,郑玉娟,等.耐高温α-淀粉酶结合Ca2+的研究[J].高等学校化学学报,1998(8):88～91.
[22]曾静,郭建军,顾斌涛,等.Ca2+结合位点对极端嗜热α-淀粉酶ApkA高温活性及热稳定性的影响[J].现代食品科技,2016,32(8):90～95,27.
[23]曾静,郭建军,袁林,等.极端嗜热α-淀粉酶ApkA的高温活性和热稳定性的优化研究[J].生物技术通报,2017,33(8):192～198.
[24]杨倩,汤斌,李松.米根霉α-淀粉酶热稳定性的理性设计[J].生物工程学报,2018,34(7):1117～1127.
[25]王爱平.淀粉酶热稳定剂及棉织物汽蒸退浆工艺的研究[D].上海:东华大学,2009.
[2]王勇,王行,贺广生,等.耐高温产淀粉酶芽孢杆菌在烟叶烘烤中降解淀粉的应用研究[J].中国烟草学报,2017,23(4):56～63.
[3]李习.高产α-淀粉酶地衣芽孢杆菌的筛选及重组质粒的构建[D].武汉:湖北工业大学,2014.
[4]岳寿松,边斐,代运章,等.产蛋白酶和淀粉酶芽孢杆菌SDYB-1的分子鉴定及酶学性能研究[J].山东农业科学,2015,47(11):54～59.
[5]MEHTA D,SATYANARAYANA T.Biochemical and molec-ular characterization of recombinant acidic and thermostable raw-starch hydrolysing α-amylase from an extreme thermophileGeobacillus thermoleovorans[J].Journal of Molecular CatalysisB:Enzymatic,2013(85-86):229～238.
[6]EMTENANI S,ASOODEH A,EMTENANI S.Gene cloningand characterization of a thermostable organic-tlerant α-amylase from Bacillus subtilis DR8806 [J].InternationalJournal of Biological Macromolecules,2015(72):290～298.
[7]SIDDIQUE F,HUSSAIN I,MAHMOOD M S,et al.Isolationand characterization of a highly thermostable alha-amylase enzyme produced by Bacillus licheniforms[J].Pakistan Journal ofAgricultural Sciences,2014,51(2):309～314.
[8]WU Xiang-rong,WANG Yu-xia,TONG Ben-ding,et al.Purifi-cation and biochemical characterization of a themostable andacid-stable alpha-amylase fromBacillus lichenifomis B4-423[J].In-ternational Journal of Biological Macromolecules,2017(109):329～337.
[9]JANA M,MAITY C,SAMANTA S,et al.Salt-independentthermophilicα-amylase from Bacillus megaterium VMB109:An efficacy testing for preparation of maltooligsaccharides[J].Industrial Crops & Products,2013,41(1):386～391.
[10]EMTENANI S,ASOODEH A,EMTENANI S.Gene cloningand characterization of a thermostable organic-tlerant α-amylase from Bacillus subtilis DR8806 [J].InternationalJournal of Biological Macromolecules,2015(72):290～298.
[11]李松,王正祥.真菌α-淀粉酶的研究进展[J].生物技术通报,2011(3):66～71.
[12]AQUINO A,JORGE J A,TERENZI H F,et al.Studies on athermostable alpha-amylase from the thermophilic fungusScytalidium thermophilum[J].Applied Microbiology and Bio-technology,2003,61(4):323～328.
[13]ALI I,AKBAR A,ANWAR M,et al.Purification and charac-terization of a polyextremophilicα-amylase from nobligate hal-ophilic aspergillus penicillioides isolate and is potential for souse with detergents[J].Biomed Research International,2015(10).
[14]朱何东,陈远钊,常峰,等.高温α-淀粉酶产生菌的筛选及酶学性质研究[J].酿酒科技,2006(5):30～32.
[15]杨自朋,张金辉,苏静,等.一株黑曲霉所产α-淀粉酶的提取及其特性研究[J].河南职工医学院学报,2006(6):431～433.
[16]田方源,汤斌,李松,等.米根霉α-淀粉酶高麦芽糖生成能力相关氨基酸的初步分析[J].食品与发酵工业,2017,43(8):27～32.
[17]何胜华,罗聪,李海梅,等.米曲霉KFRI 888产中性蛋白酶、a-淀粉酶酶学性质的研究[J].食品工业科技,2006(9):49～50+53.
[18]罗文杰.耐热酸性淀粉酶筛选及酶学特性研究[D].杭州:浙江工业大学,2008.
[19]张强,刘成君,蒋芳,等.耐高温α-淀粉酶产生菌的分离鉴定及发酵条件与酶性质研究[J].食品与发酵工业,2005(2):34～37.
[20]李祝.嗜热脂肪芽孢杆菌α-淀粉酶的异源表达及热稳定性改造[D].无锡:江南大学,2018.
[21]赵荧,费小芳,郑玉娟,等.耐高温α-淀粉酶结合Ca2+的研究[J].高等学校化学学报,1998(8):88～91.
[22]曾静,郭建军,顾斌涛,等.Ca2+结合位点对极端嗜热α-淀粉酶ApkA高温活性及热稳定性的影响[J].现代食品科技,2016,32(8):90～95,27.
[23]曾静,郭建军,袁林,等.极端嗜热α-淀粉酶ApkA的高温活性和热稳定性的优化研究[J].生物技术通报,2017,33(8):192～198.
[24]杨倩,汤斌,李松.米根霉α-淀粉酶热稳定性的理性设计[J].生物工程学报,2018,34(7):1117～1127.
[25]王爱平.淀粉酶热稳定剂及棉织物汽蒸退浆工艺的研究[D].上海:东华大学,2009.