药用植物miRNA调控次生代谢的研究进展
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摘要
miRNA是一类长度为20~25 nt的内源性非编码小分子单链RNA,对药用植物的生长发育、次生代谢、生物胁迫、非生物胁迫等具有重要的调控作用。次生代谢产物是药用植物的主要活性成分,目前从药用植物中鉴定出多种miRNA对次生代谢生物合成、上游信号转导具有调控功能。本文综述了近年来药用植物miRNA调控次生代谢的研究情况,既为培育优质高产的中药材积累基础研究资料,也为药用植物的临床应用开拓思路。
Mi RNAs are endogenous single-stranded non-coding small(19~25 nt) RNAs that play the key roles in plant development, secondary metabolism, and biotic/abiotic stress. Moreover, secondary metabolites are the main active ingredients of medicinal plants. At present, a variety of miRNAs has been identified from the medicinal plants to regulate the secondary metabolic biosynthesis and upstream signal transduction. In this paper, the recent research on the secondary metabolism of medicinal plants by miRNAs is reviewed, which is of great significance to cultivate high quality and high yield of traditional Chinese medicine, and also for the clinical use of medicinal plants.
Mi RNAs are endogenous single-stranded non-coding small(19~25 nt) RNAs that play the key roles in plant development, secondary metabolism, and biotic/abiotic stress. Moreover, secondary metabolites are the main active ingredients of medicinal plants. At present, a variety of miRNAs has been identified from the medicinal plants to regulate the secondary metabolic biosynthesis and upstream signal transduction. In this paper, the recent research on the secondary metabolism of medicinal plants by miRNAs is reviewed, which is of great significance to cultivate high quality and high yield of traditional Chinese medicine, and also for the clinical use of medicinal plants.
引文
[1]Lee RC,Feinbaum RL,Ambros V.The C.elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.Cell,1993,75(5):843-854
[2]Reinhart BJ,Slack FJ,Basson M,et al.The 21 nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans.Nature,2000,403(6772):901-906
[3]Reinhart BJ,Weinstein EG,Rhoades MW,et al.Micro RNAs in plants.Genes Dev,2002,16(13):1616-1626
[4]Sharma D,Tiwari M,Pandey A,et al.Micro RNA858 is a potential regulator of phenylpropanoid pathway and plant development.Plant Physiol,2016,171(2):944
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[8]Li C,Li D,Jiang L,et al.Characterization of the polyphenol oxidase gene family reveals a novel micro RNA involved in posttranscriptional regulation of PPOs in Salvia miltiorrhiza.Sci Rep,2017,7:44622
[9]Yang Y,Chen X,Chen J,et al.Identification of novel andconserved micro RNAs in Rehmannia glutinosa L by Solexa sequencing.Plant Mol Biol Rep,2011,29(4):986-996
[10]Pani A,Mahapatra RK.Computational identification of micro RNAs and their targets in Catharanthus roseus expressed sequence tags.Genomics Data,2013,1:2-6
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[12]Fan R,Li Y,Li C,et al.Differential micro RNA analysis of glandular trichomes and young leaves in Xanthium strumarium L.reveals their putative roles in regulating terpenoid biosynthesis.PLo S ONE,2015,10(9):e0139002
[13]Fan K,Fan D,Ding Z,et al.Cs-mi R156 is involved in the nitrogen form regulation of catechins accumulation in tea plant(Camellia sinensis L.).Plant Physiol Biochem,2015,97:350-360
[14]Mathiyalagan R,Subramaniyam S,Natarajan S,et al.Insilicoprofiling of micro RNAs in Korean ginseng(Panax ginseng Meyer).J Ginseng Res,2013,37(2):227-247
[15]Zhang M,Dong Y,Nie L,et al.High-throughput sequencing reveals mi RNA effects on the primary and secondary production properties in long-term subcultured Taxus cells.Front Plant Sci,2015,6:604
[16]Hao DC,Yang L,Xiao PG,et al.Identification of Taxusmicro RNAs and their targets with high-throughput sequencing and degradome analysis.Physiol Plant,2012,146:388-403
[17]Melissa MLW,Charles HC,Ratnam W.Identification of lignin genes and regulatory sequences involved in secondary cell wall formation in Acacia auriculiformis and Acacia mangium via de novo transcriptome sequencing.BMC Genomics,2011,12:342
[18]周丹丹,俞嘉宁.植物细胞中瞬时表达系统的建立及研究进展.中国农学通报,2013,24:151-156
[19]Vashisht I,Mishra P,Pal T,et al.Mining NGS transcriptomes for mi RNAs and dissecting their role in regulating growth,development,and secondary metabolites production in different organs of a medicinal herb,Picrorhiza kurroa.Planta,2015,241:1255-1268
[20]Wu B,Li Y,Yan H,et al.Comprehensive transcriptome analysis reveals novel genes involved in cardiac glycoside biosynthesis and mlnc RNAs associated with secondary metabolism and stress response in Digitalis purpurea.BMC Genomics,2012,13:1471-2164
[21]Nagatoshi M,Terasaka K,Nagatsu A,et al.An iridoid-specific glucosyltransferase from Gardenia jasminoides.Biol Chem,2011,286:32866-32874
[22]De Waal A,Meijer AH,Verpoorte R.Strictosidine synthase from Catharanthus roseus:purification and characterization of multiple forms.Biochem,1995,306:571-580
[23]Mathews H,Clendennen SK,Caldwell CG,et al.Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis,modification,and transport.Plant Cell,2003,15:1689-1703
[24]Goff SA,Cone KC,Chandle VL.Functional analysis of the transcriptional activator enconded by the maize B gene:evidence for a direct functional interaction between two classes of regulatory proteins.Genes Dev,1992,6(5):864-875
[25]Mol J,Grotewold E,Koes R.How genes paint flowers and seeds.Trends Plant Sci,1998,3:212-217
[26]Gou JY,Felippes FF,Liu CJ,et al.Negative regulation of anthocyanin biosynthesis in Arabidopsis by a mi R156-targeted SPL transcription factor.Plant Cell,2011,23:1512-1522
[27]曾幼玲,杨瑞瑞.植物mi RNA的生物学特性及在环境胁迫中的作用.中国农业科学,2016,19:3671-3682
[28]Zhou M,Luo H.Micro RNA-mediated gene regulation:Potential applications for plant genetic engineering.Plant Mol Biol,2013,83(1/2):59-75
[29]Lee Y,Ahn C,Han J,et al.The nuclear RNase III Drosha initiates micro RNA processing.Nature,2003,425(6956):415-419
[30]Rogers K,Chen X.Biogenesis,turnover,and mode of action of plant micro RNAs.Plant Cell,2013,25:2383-2399
[31]Voinnet O.Origin,biogenesis,and activity of plant micro RNAs.Cell,2009,136(4):669-687
[32]Jones-Rhoades MW,Bartel DP,Bartel B.Micro RNAs and their regulatory roles in plants.Annu Rev Plant Biol,2006,57:19-53
[33]Wahid F,Shehzad A,Khan T,et al.Micro RNAs:Synthesis,mechanism,function,and recent clinical trials.Biochim Biophys Acta,2010,1803(11):1231-1243
[34]Ambros V.micro RNAs:tiny regulators with great potential.Cell,2001,107(7):823-826
[35]Kumar R.Role of micro RNAs in biotic and abiotic stress responses in crop plants.Appl Biochem Biotechnol,2014,174(1):93-115
[36]Kusenda B,Mraz M,Mayer J,et al.Micro RNA biogenesis,functionality and cancer relevance.Biomedical Papers(Olomouc),2006,150(2):205-215
[37]Rhoades MW,Reinhart BJ,Lim LP,et al.Prediction of plant micro RNA targets.Cell,2002,110(4):513-520
[38]Allen E,Xie Z,Gustafson AM,et al.Micro RNA-directed phasing during trans-acting si RNA biogenesis in plants.2005,Cell,121(2):207-221
[39]Schwab R,Palatnik JF,Riester M,et al.Specific effects of micro RNAs on the plant transcriptome.Dev Cell,2005,8(4):517-527
[40]Dai X,Zhao PX.ps RNATarget:a plant small RNA target analysis server.Nucleic Acids Res,2011,39:W155-W159
[41]杨江伟.马铃薯干旱相关micro RNA的鉴定及功能研究.甘肃农业大学博士论文,2015,20-21
[42]秦力.芦笋雌雄花发育转录组分析及性别决定相关mi RNA靶基因的鉴定.浙江大学硕士论文,2016,67
[43]南文智,吴嫚,于霁雯,等.利用高通量测序技术鉴定棉纤维发育相关mi RNAs及其靶基因.棉花学报,2013,04:300-308
[44]Qiao Y,Zhang J,Zhang J,et al.Integrated RNA-seq and s RNA-seq analysis reveals mi RNA effects on secondary metabolism in Solanum tuberosum L.Mol Geneti Genomics,2017,292(1):37
[45]Boke H,Ozhuner E,Turktas M,et al.Regulation of the alkaloid biosynthesis by mi RNA in opium poppy.Plant Biotechnol J,2015,13:409-420
[46]Shao F,Zhang Q,Liu H,et al.Genome-Wide identification and analysis of micro RNAs involved in Witches'-Broom phytoplasma response in Ziziphus jujuba.Plos One,2016,11(11):e0166099
[47]宋长年.枳和柑橘micro RNA及其靶基因的识别、鉴定与表达分析.南京农业大学博士论文,2011,39-55
[48]赵晓锋.玉米雌穗发育相关mi RNAs靶基因的降解组分析.河南农业大学硕士论文,2015,12-14
[49]Sleytr UB,Messner P,Pum D,et al.Crystalline bacterial cell surface layers(s layers):from supramolecular cell structure to biomimetics and nanotechnology.Angew Chem Int Ed Engl,1999,30(29):1034-1054
[50]徐烨,刘雅婷,代文琼,等.几种主要的RACE技术及应用.中国农业科技导报,2012,02:81-87
[51]Feng JL,Liu SS,Wang MN,et al.Identification of micro RNAs and their targets in tomato infected with Cucumber mosaic virus based on deep sequencing.Planta,2014,240(6):1335-1352
[52]石林.基于在线工具和SVM的植物mi RNA靶基因集成预测研究.大连理工大学硕士论文,2014,13-31
[53]何炫程,吕鹤书,黄捷飞,等.植物mi RNA靶基因验证研究进展.生物技术进展,2017,02:102-105
[54]高颖,房德敏.骨碎补黄酮类化合物的研究进展与开发前.中草药,2009,40(2):323-326
[55]占爱瑶,由香玲,詹亚光.植物萜类化合物的生物合成及应用.生物技术通讯,2010,21(1):131-135
[56]Yang PY,Hsieh PL,Wang TH,et al.Andrographolide impedes cancer stemness and enhances radio-sensitivity in oral carcinomas via mi R-218 activation.Oncotarget,2017,8(3):4196-4207
[57]王尧龙.金银花产地鉴定mi RNA及SNP标记的筛选.昆明理工大学硕士论文,2016,87-103
[2]Reinhart BJ,Slack FJ,Basson M,et al.The 21 nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans.Nature,2000,403(6772):901-906
[3]Reinhart BJ,Weinstein EG,Rhoades MW,et al.Micro RNAs in plants.Genes Dev,2002,16(13):1616-1626
[4]Sharma D,Tiwari M,Pandey A,et al.Micro RNA858 is a potential regulator of phenylpropanoid pathway and plant development.Plant Physiol,2016,171(2):944
[5]田铃,嵇保中,刘曙雯,等.甲基转移酶的功能与分类.生命的化学,2007,27(5):425-427
[6]Luo QJ,Mittal A,Jia F,et al.An autoregulatory feedback loop involving PAP1 and TAS4 in response to sugars in Arabidopsis.Plant Mol Biol,2012,80:117-129
[7]雷凯健,刘浩.植物调控枢纽mi R156及其靶基因SPL家族研究进展.生命的化学,2016,36(01):13-20
[8]Li C,Li D,Jiang L,et al.Characterization of the polyphenol oxidase gene family reveals a novel micro RNA involved in posttranscriptional regulation of PPOs in Salvia miltiorrhiza.Sci Rep,2017,7:44622
[9]Yang Y,Chen X,Chen J,et al.Identification of novel andconserved micro RNAs in Rehmannia glutinosa L by Solexa sequencing.Plant Mol Biol Rep,2011,29(4):986-996
[10]Pani A,Mahapatra RK.Computational identification of micro RNAs and their targets in Catharanthus roseus expressed sequence tags.Genomics Data,2013,1:2-6
[11]Prakash P,Ghosliya D,Gupta V.Identification of conserved and novel micro RNAs in Catharanthus roseus by deep sequencing and computational prediction of their potential targets.Gene,2015,554:181-195
[12]Fan R,Li Y,Li C,et al.Differential micro RNA analysis of glandular trichomes and young leaves in Xanthium strumarium L.reveals their putative roles in regulating terpenoid biosynthesis.PLo S ONE,2015,10(9):e0139002
[13]Fan K,Fan D,Ding Z,et al.Cs-mi R156 is involved in the nitrogen form regulation of catechins accumulation in tea plant(Camellia sinensis L.).Plant Physiol Biochem,2015,97:350-360
[14]Mathiyalagan R,Subramaniyam S,Natarajan S,et al.Insilicoprofiling of micro RNAs in Korean ginseng(Panax ginseng Meyer).J Ginseng Res,2013,37(2):227-247
[15]Zhang M,Dong Y,Nie L,et al.High-throughput sequencing reveals mi RNA effects on the primary and secondary production properties in long-term subcultured Taxus cells.Front Plant Sci,2015,6:604
[16]Hao DC,Yang L,Xiao PG,et al.Identification of Taxusmicro RNAs and their targets with high-throughput sequencing and degradome analysis.Physiol Plant,2012,146:388-403
[17]Melissa MLW,Charles HC,Ratnam W.Identification of lignin genes and regulatory sequences involved in secondary cell wall formation in Acacia auriculiformis and Acacia mangium via de novo transcriptome sequencing.BMC Genomics,2011,12:342
[18]周丹丹,俞嘉宁.植物细胞中瞬时表达系统的建立及研究进展.中国农学通报,2013,24:151-156
[19]Vashisht I,Mishra P,Pal T,et al.Mining NGS transcriptomes for mi RNAs and dissecting their role in regulating growth,development,and secondary metabolites production in different organs of a medicinal herb,Picrorhiza kurroa.Planta,2015,241:1255-1268
[20]Wu B,Li Y,Yan H,et al.Comprehensive transcriptome analysis reveals novel genes involved in cardiac glycoside biosynthesis and mlnc RNAs associated with secondary metabolism and stress response in Digitalis purpurea.BMC Genomics,2012,13:1471-2164
[21]Nagatoshi M,Terasaka K,Nagatsu A,et al.An iridoid-specific glucosyltransferase from Gardenia jasminoides.Biol Chem,2011,286:32866-32874
[22]De Waal A,Meijer AH,Verpoorte R.Strictosidine synthase from Catharanthus roseus:purification and characterization of multiple forms.Biochem,1995,306:571-580
[23]Mathews H,Clendennen SK,Caldwell CG,et al.Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis,modification,and transport.Plant Cell,2003,15:1689-1703
[24]Goff SA,Cone KC,Chandle VL.Functional analysis of the transcriptional activator enconded by the maize B gene:evidence for a direct functional interaction between two classes of regulatory proteins.Genes Dev,1992,6(5):864-875
[25]Mol J,Grotewold E,Koes R.How genes paint flowers and seeds.Trends Plant Sci,1998,3:212-217
[26]Gou JY,Felippes FF,Liu CJ,et al.Negative regulation of anthocyanin biosynthesis in Arabidopsis by a mi R156-targeted SPL transcription factor.Plant Cell,2011,23:1512-1522
[27]曾幼玲,杨瑞瑞.植物mi RNA的生物学特性及在环境胁迫中的作用.中国农业科学,2016,19:3671-3682
[28]Zhou M,Luo H.Micro RNA-mediated gene regulation:Potential applications for plant genetic engineering.Plant Mol Biol,2013,83(1/2):59-75
[29]Lee Y,Ahn C,Han J,et al.The nuclear RNase III Drosha initiates micro RNA processing.Nature,2003,425(6956):415-419
[30]Rogers K,Chen X.Biogenesis,turnover,and mode of action of plant micro RNAs.Plant Cell,2013,25:2383-2399
[31]Voinnet O.Origin,biogenesis,and activity of plant micro RNAs.Cell,2009,136(4):669-687
[32]Jones-Rhoades MW,Bartel DP,Bartel B.Micro RNAs and their regulatory roles in plants.Annu Rev Plant Biol,2006,57:19-53
[33]Wahid F,Shehzad A,Khan T,et al.Micro RNAs:Synthesis,mechanism,function,and recent clinical trials.Biochim Biophys Acta,2010,1803(11):1231-1243
[34]Ambros V.micro RNAs:tiny regulators with great potential.Cell,2001,107(7):823-826
[35]Kumar R.Role of micro RNAs in biotic and abiotic stress responses in crop plants.Appl Biochem Biotechnol,2014,174(1):93-115
[36]Kusenda B,Mraz M,Mayer J,et al.Micro RNA biogenesis,functionality and cancer relevance.Biomedical Papers(Olomouc),2006,150(2):205-215
[37]Rhoades MW,Reinhart BJ,Lim LP,et al.Prediction of plant micro RNA targets.Cell,2002,110(4):513-520
[38]Allen E,Xie Z,Gustafson AM,et al.Micro RNA-directed phasing during trans-acting si RNA biogenesis in plants.2005,Cell,121(2):207-221
[39]Schwab R,Palatnik JF,Riester M,et al.Specific effects of micro RNAs on the plant transcriptome.Dev Cell,2005,8(4):517-527
[40]Dai X,Zhao PX.ps RNATarget:a plant small RNA target analysis server.Nucleic Acids Res,2011,39:W155-W159
[41]杨江伟.马铃薯干旱相关micro RNA的鉴定及功能研究.甘肃农业大学博士论文,2015,20-21
[42]秦力.芦笋雌雄花发育转录组分析及性别决定相关mi RNA靶基因的鉴定.浙江大学硕士论文,2016,67
[43]南文智,吴嫚,于霁雯,等.利用高通量测序技术鉴定棉纤维发育相关mi RNAs及其靶基因.棉花学报,2013,04:300-308
[44]Qiao Y,Zhang J,Zhang J,et al.Integrated RNA-seq and s RNA-seq analysis reveals mi RNA effects on secondary metabolism in Solanum tuberosum L.Mol Geneti Genomics,2017,292(1):37
[45]Boke H,Ozhuner E,Turktas M,et al.Regulation of the alkaloid biosynthesis by mi RNA in opium poppy.Plant Biotechnol J,2015,13:409-420
[46]Shao F,Zhang Q,Liu H,et al.Genome-Wide identification and analysis of micro RNAs involved in Witches'-Broom phytoplasma response in Ziziphus jujuba.Plos One,2016,11(11):e0166099
[47]宋长年.枳和柑橘micro RNA及其靶基因的识别、鉴定与表达分析.南京农业大学博士论文,2011,39-55
[48]赵晓锋.玉米雌穗发育相关mi RNAs靶基因的降解组分析.河南农业大学硕士论文,2015,12-14
[49]Sleytr UB,Messner P,Pum D,et al.Crystalline bacterial cell surface layers(s layers):from supramolecular cell structure to biomimetics and nanotechnology.Angew Chem Int Ed Engl,1999,30(29):1034-1054
[50]徐烨,刘雅婷,代文琼,等.几种主要的RACE技术及应用.中国农业科技导报,2012,02:81-87
[51]Feng JL,Liu SS,Wang MN,et al.Identification of micro RNAs and their targets in tomato infected with Cucumber mosaic virus based on deep sequencing.Planta,2014,240(6):1335-1352
[52]石林.基于在线工具和SVM的植物mi RNA靶基因集成预测研究.大连理工大学硕士论文,2014,13-31
[53]何炫程,吕鹤书,黄捷飞,等.植物mi RNA靶基因验证研究进展.生物技术进展,2017,02:102-105
[54]高颖,房德敏.骨碎补黄酮类化合物的研究进展与开发前.中草药,2009,40(2):323-326
[55]占爱瑶,由香玲,詹亚光.植物萜类化合物的生物合成及应用.生物技术通讯,2010,21(1):131-135
[56]Yang PY,Hsieh PL,Wang TH,et al.Andrographolide impedes cancer stemness and enhances radio-sensitivity in oral carcinomas via mi R-218 activation.Oncotarget,2017,8(3):4196-4207
[57]王尧龙.金银花产地鉴定mi RNA及SNP标记的筛选.昆明理工大学硕士论文,2016,87-103