獾子油对小鼠乙醇性胃溃疡的保护作用
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摘要
目的研究獾子油对小鼠胃溃疡的保护作用。方法以乙醇致小鼠胃溃疡为模型,以奥美拉唑作为阳性药对照,通过计算溃疡指数,测定小鼠胃液PH值,胃组织匀浆中一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量,研究獾子油高、中、低剂量(0.2、0.1和0.05 mL·10 g~(-1))对小鼠胃溃疡的保护作用及其与剂量的关系。结果与模型组比较,各剂量组的獾子油对乙醇致小鼠胃溃疡均有一定的改善作用,能抑制胃酸的分泌,减小溃疡面积,降低胃组织中NO的含量,使SOD、MDA含量升高,而且随着浓度的增加作用效果逐渐增强,其中高剂量组有显著性差异(P<0.05)。结论獾子油对乙醇致小鼠胃溃疡具有一定的保护作用,且高剂量效果最好。
Objective The protective effect of badger oil on gastric ulcer in mice was researched.Methods The mice were treated with ethanol-induced gastric ulcer as model and omeprazole was used as positive drug.The PH value of gastric juice was determined by calculating the ulcer index.The contents of nitric oxide(NO),superoxide dismutase(0.2 mL·10 g~(-1),0.1 mL·10 g~(-1),0.05 mL·10 g~(-1)) on the gastric ulcer of mice were studied.The effects of high,medium and low dose(0.2 mL·10 g~(-1),0.1 mL·10 g~(-1),0.05 mL·10 g~(-1)) on the gastric ulcer of mice were studied.Results Compared with the model group,the badger oil of each dose group had some effect on the gastric ulcer induced by ethanol,which could inhibit the secretion of gastric acid,reduce the area of ulcer and decrease the content of NO in gastric tissue(P<0.05).Conclusion Badger oil has a certain protective effect on ethanol-induced gastric ulcer in mice,and high dose has the best effect.
Objective The protective effect of badger oil on gastric ulcer in mice was researched.Methods The mice were treated with ethanol-induced gastric ulcer as model and omeprazole was used as positive drug.The PH value of gastric juice was determined by calculating the ulcer index.The contents of nitric oxide(NO),superoxide dismutase(0.2 mL·10 g~(-1),0.1 mL·10 g~(-1),0.05 mL·10 g~(-1)) on the gastric ulcer of mice were studied.The effects of high,medium and low dose(0.2 mL·10 g~(-1),0.1 mL·10 g~(-1),0.05 mL·10 g~(-1)) on the gastric ulcer of mice were studied.Results Compared with the model group,the badger oil of each dose group had some effect on the gastric ulcer induced by ethanol,which could inhibit the secretion of gastric acid,reduce the area of ulcer and decrease the content of NO in gastric tissue(P<0.05).Conclusion Badger oil has a certain protective effect on ethanol-induced gastric ulcer in mice,and high dose has the best effect.
引文
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[13] 王海燕,刘亚明,李海燕,等.高良姜油对胃溃疡小鼠模型血清胃动素、生长抑素、P 物质、血管活性肠肽的影响[J].中国实验方剂学杂志,2011,17(4):105.
[14] 瞿春莹,李定国,汪余勤,等.壳聚糖对胃溃疡大鼠血清中MDA、SOD 及 GSH-PX 的影响 [J].上海医药,2008,29(5):219.
[15] 邓潇潇,赵欣.不同浓度虫茶对 SD 大鼠的胃损伤预防效果评价[J].北京联合大学学报:自然科学版,2013,27(2):64.
[16] 严海密,高锦孝,胡佩琪,等.消化性溃疡患者血清、胃液一氧化氮含量[J].世界华人消化杂志,1999,7(7):627.
[2] CARDOSO C R,SOUZA M A,FERRO E A,et al.Influence of topical administration of n-3 and n-6 essntial and n-9 nonessential fatty acids on the healing of cutaneous wounds[J].Wound Repair Regener,2004,12(2):235.
[3] 陈恒冲,罗晶,李景辉,等.獾油质量标准研究[J].中国民族民间医药杂志,2009,18(19):21.
[4] 朱琳,宋起滨,窦德强,等.獾子油的化学成分研究[J].中国实验方剂学杂志,2013,11(9):22.
[5] 赵明宏,郭涛,刘玉兰,等.安胃微丸抗胃溃疡作用[J].沈阳药科大学学报,2002,19(2):118-121.
[6] 陈志东.消化性胃溃疡模型在研究新药中的应用[J].现代中西医结合杂志,2006,22(2):25.
[7] 徐淑云.药理实验方法学:第3版[M].北京:人民卫生出版社,2002:911.
[8] 刘巨源,刘春霞.溃疡散对实验性大鼠胃溃疡黏膜PGE2含量及血流量的影响[J].中国中医药科技,2001,8(5):290.
[9] 丁志山,蒋福升.姜黄素对动物实验性胃溃疡的影响研究[J].中华中医药学刊,2008,26(10):2225.
[10] 于海玲,郭建鹏,孙连平.老鹳草提取物对实验性胃溃疡小鼠胃黏膜的保护作用机制研究[J].延边大学医学学报,2007,30(1):29.
[11] 刘莹,崔炯谟.白及超微粉对大鼠实验性胃溃疡的影响[J].中草药,2008,39(3):397.
[12] 高静,杨兰泽.消化性溃疡患者血中MDA、SOD及GSH-PX的变化[J].中华实用中西医杂志,2005,18(23):1781.
[13] 王海燕,刘亚明,李海燕,等.高良姜油对胃溃疡小鼠模型血清胃动素、生长抑素、P 物质、血管活性肠肽的影响[J].中国实验方剂学杂志,2011,17(4):105.
[14] 瞿春莹,李定国,汪余勤,等.壳聚糖对胃溃疡大鼠血清中MDA、SOD 及 GSH-PX 的影响 [J].上海医药,2008,29(5):219.
[15] 邓潇潇,赵欣.不同浓度虫茶对 SD 大鼠的胃损伤预防效果评价[J].北京联合大学学报:自然科学版,2013,27(2):64.
[16] 严海密,高锦孝,胡佩琪,等.消化性溃疡患者血清、胃液一氧化氮含量[J].世界华人消化杂志,1999,7(7):627.