脂肪源性血管基质成分对皮肤光老化的影响
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摘要
目的:观察人脂肪源性血管基质成分(stromal vascular fractions,SVF)对中波紫外线(ultraviolet B,UVB)诱导的裸大鼠皮肤光老化的影响。方法:6周龄裸大鼠随机分为正常对照组及实验组,实验组持续UVB照射8周建立皮肤光老化模型并随机分为模型组,以及SVF低剂量(low-dose,LD)、中剂量(middle-dose,MD)和高剂量(high-dose,HD)(100μL悬液中的细胞数分别为10~4、10~5和10~6)治疗组;采用酶消化法提取健康女性脂肪组织中的SVF,分别取低、中、高剂量的SVF悬液注射于裸大鼠背部皮下;模型组注射相同剂量的生理盐水,正常对照组不做任何处理;7 d后取材,观察注射部位表皮厚度及结构的改变;28 d后取材观察注射部位真皮厚度及结构的改变。结果:SVF治疗7 d后,中、高剂量治疗组裸大鼠皮肤较实验对照组表皮层厚度减小、角质层比例下降、基底层处于异常增殖状态的细胞核减少(P<0.05)。治疗28 d后,中剂量及高剂量治疗组Ⅰ型胶原蛋白mRNA相对表达量增加,Ⅲ型胶原蛋白及基质金属蛋白酶-3(matrix metalloproteinases-3,MMP-3)mRNA相对表达量下降(P<0.05),高剂量治疗组皮肤真皮层厚度增加(P<0.05)。结论:SVF具有抗皮肤光老化的潜能,有潜在的临床应用价值。
AIM: To investigate the anti-aging effect of the stromal vascular fractions( SVF) injection in photo-aging skin of nude rats. METHODS: Six-week-old nude rats were divided into control group and photoaging group. The nude rats in photoaging group were continuously irradiated with ultraviolet B( UVB) for 8 weeks to build the photoaging skin model,and were randomly divided into placebo group,and low-dose( LD),middle-dose( MD) and high-dose( HD)SVF treatment groups. The human SVF was obtained by trypsin digestion from the adipose tissue in healthy females,and was subcutaneously injected into the back skin of photoaging nude rats in LD,MD and HD SVF treatment groups( 10~4,10~5 and 10~6cells in 100 μL suspension,respectively). The animals in photoaging skin model group were treated with placebo.Control group did not have any intervention. The changes in the thickness of epidermis and histology after 7 d and the thickness of the dermis and histology after 28 d were examined. RESULTS: The thickness of the epidermis was thinner in MD and HD SVF treatment groups than that in photoaging skin model group after 7 d( P < 0. 05). The percentage of stratum corneum and the abnormal proliferation of the epidermal basal cell layer were reduced( P < 0. 05). After 28 d,MD and HD SVF treatment groups showed that the content of collagen Ⅰ was higher than that in photoaging skin model group( P <0. 05),and the contents of collagen Ⅲ and MMP3 were decreased( P < 0. 05). The dermis in HD group was thicker than that in photoaging skin model group( P < 0. 05). CONCLUSION: SVF may have anti-aging potential in photoaging skin and also has clinical application value.
AIM: To investigate the anti-aging effect of the stromal vascular fractions( SVF) injection in photo-aging skin of nude rats. METHODS: Six-week-old nude rats were divided into control group and photoaging group. The nude rats in photoaging group were continuously irradiated with ultraviolet B( UVB) for 8 weeks to build the photoaging skin model,and were randomly divided into placebo group,and low-dose( LD),middle-dose( MD) and high-dose( HD)SVF treatment groups. The human SVF was obtained by trypsin digestion from the adipose tissue in healthy females,and was subcutaneously injected into the back skin of photoaging nude rats in LD,MD and HD SVF treatment groups( 10~4,10~5 and 10~6cells in 100 μL suspension,respectively). The animals in photoaging skin model group were treated with placebo.Control group did not have any intervention. The changes in the thickness of epidermis and histology after 7 d and the thickness of the dermis and histology after 28 d were examined. RESULTS: The thickness of the epidermis was thinner in MD and HD SVF treatment groups than that in photoaging skin model group after 7 d( P < 0. 05). The percentage of stratum corneum and the abnormal proliferation of the epidermal basal cell layer were reduced( P < 0. 05). After 28 d,MD and HD SVF treatment groups showed that the content of collagen Ⅰ was higher than that in photoaging skin model group( P <0. 05),and the contents of collagen Ⅲ and MMP3 were decreased( P < 0. 05). The dermis in HD group was thicker than that in photoaging skin model group( P < 0. 05). CONCLUSION: SVF may have anti-aging potential in photoaging skin and also has clinical application value.
引文
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[22]Schwartz E,Cruickshank FA,Christensen CC,et al.Collagen alterations in chronically sun-damaged human skin[J].Photochem Photobiol,1993,58(6):841-844.
[23]Kim JH,Jung M,Kim HS,et al.Adipose-derived stem cells as a new therapeutic modality for ageing skin[J].Exp Dermatol,2011,20(5):383-387.
[24]Kim HH,Lee MJ,Lee SR,et al.Augmentation of UV-induced skin wrinkling by infrared irradiation in hairless mice[J].Mech Ageing Dev,2005,126(11):1170-1177.
[25]郑锦芬,陆春,赖维.基质金属蛋白酶与皮肤光老化[J].中华医学美学美容杂志,2012,18(1):75-77.
[2]居永芳.皮肤老化机制的研究进展[J].中国麻风皮肤病杂志,2006,22(2):143-146.
[3]刘玮.皮肤光老化[J].临床皮肤科杂志,2003,32(7):424-426.
[4]冯燕艳,普雄明.皮肤自然衰老及光老化[J].国外医学:皮肤性病学分册,2004,30(6):354-356.
[5]Fisher GJ,Wang ZQ,Datta SC,et al.Pathophysiology of premature skin aging induced by uhraviolet light[J].N Engl J Med,1997,337(20):1419-1428.
[6]Zuk PA,Zhu M,Mizuno H,et al.Multilineage cells from human adipose tissue:implications for cell-based therapies[J].Tissue Eng,2001,7(2):211-218.
[7]Rehman J,Traktuev D,Li J,et al.Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells[J].Circulation,2004,109(10):1292-1298.
[8]Kim WS,Park BS,Sung JH.The wound-healing and antioxidant effects of adipose-derived stem cells[J].Expert Opin Biol Ther,2009,9(7):879-887.
[9]Kim BH,Son WC,Yim CO,et al.Anti-wrinkle effects of adipose tissue-derived mesenchymal stem cells in a UV-irradiated hairless mouse model[J].Tissue Eng Regen Med,2010,7(5):583-591.
[10]Kim WS,Park BS,Park SH,et al.Anti-wrinkle effect of adipose-derived stem cell:activation of dermal fibroblast by secretory factors[J].J Dermatol Sci,2009,53(2):96-102.
[11]Kim WS,Park BS,Sung JH.Protective role of adiposederived stem cells and their soluble factors in photo-aging[J].Arch Dermatol Res,2009,301(5):329-336.
[12]Kim WS,Park SH,Ahn SJ,et al.Whitening effect of adipose-derived stem cells a critical role of TGF-beta 1[J].Biol Pharm Bull,2008,31(4):606-610.
[13]Park BS,Jang KA,Sung JH,et al.Adipose-derived stem cells and their secretory factors as a promising therapy for skin aging[J].Dermatol Surg,2008,34(10):1323-1326.
[14]Alharbi Z,Oplander C,Almakadi S,et al.Conventional vs.micro-fat harvesting:how fat harvesting technique affects tissue-engineering approaches using adipose tissuederived stem/stromal cells[J].J Plast Reconstr Aesthet Surg,2013,66(9):1271-1278.
[15]Bissett DL,Hannon DP,Orr TV.An animal model of solar-aged skin:Histological 7 and physical 7 and visible changes in UV-irradiated hairless mouse skin[J].Photochem Photobiol,1987,46(3):367-378.
[16]Klumpers DD,Mooney DJ,Smit TH.From skeletal development to tissue engineering:lessons from the micromass assay[J].Tissue Eng Part B Rev,2015,21(5):427-437.
[17]Zhou L,Xia J,Qiu X,et al.In vitro evaluation of endothelial progenitor cells from adipose tissue as potential angiogenic cell sources for bladder angiogenesis[J].PLo S One,2015,10(2):e0117644.
[18]潘敏洪,周晓军,姜少军.皮肤角质层及其损伤的研究进展[J].医学研究报,2006,19(11):1036-1039.
[19]Karmer E,Herman O,Frand J,et al.Ki67 as a biologic marker of basal cell carcinoma:a retrospective study[J].Isr Med Assoc J,2014,16(4):229-232.
[20]Ichihasi M,Ueda M,Bidiyanto A,et al.UV-induced skin damage[J].Toxicology,2003,189(1-2):21-39.
[21]石钰,李利,王曦.老化皮肤的胶原与弹性组织变化研究[J].中国美容医学,2007,16(6):727-730.
[22]Schwartz E,Cruickshank FA,Christensen CC,et al.Collagen alterations in chronically sun-damaged human skin[J].Photochem Photobiol,1993,58(6):841-844.
[23]Kim JH,Jung M,Kim HS,et al.Adipose-derived stem cells as a new therapeutic modality for ageing skin[J].Exp Dermatol,2011,20(5):383-387.
[24]Kim HH,Lee MJ,Lee SR,et al.Augmentation of UV-induced skin wrinkling by infrared irradiation in hairless mice[J].Mech Ageing Dev,2005,126(11):1170-1177.
[25]郑锦芬,陆春,赖维.基质金属蛋白酶与皮肤光老化[J].中华医学美学美容杂志,2012,18(1):75-77.