山羊精液冷冻保存研究进展
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摘要
精液的冷冻保存可使精液长期保存和运输,扩大精液的使用范围,使配种不受时间、地域、种畜生命的限制。充分利用优秀种公畜的遗传资源,加快育种工作进程,提高优良公畜的配种效率和种用价值。随着养羊业的迅速发展,冷冻山羊精液亟待应用于生产。山羊精液的冷冻保存是一个复杂的过程,为了获得理想的保存效果,需要平衡多种因素。从山羊精液冷冻保存剂成分、冷冻解冻的过程、冷冻过程的影响因素等方面予以综述,旨在为今后的精液冷冻保存研究工作提供一定的参考依据。
Frozen semen can be long-distance transported and preserved, which can be used in a wide range, so that the breeding is not limited by time, region and livestock life. Making full use of the excellent genetic resources of male animals can speed up the breeding process and improve breeding efficiency and value of male animals. With the rapid development of the goat industry, frozen goat semen is urgent to be used in production. However, freezing goat semen is a complicated process. To obtain desired preservative effect, many factors should be taken into consideration. This article reviews the agents of frozen semen, process of freezing and thawing and influential factors of freezing process, aiming to provide a reference for the future semen frozen research.
Frozen semen can be long-distance transported and preserved, which can be used in a wide range, so that the breeding is not limited by time, region and livestock life. Making full use of the excellent genetic resources of male animals can speed up the breeding process and improve breeding efficiency and value of male animals. With the rapid development of the goat industry, frozen goat semen is urgent to be used in production. However, freezing goat semen is a complicated process. To obtain desired preservative effect, many factors should be taken into consideration. This article reviews the agents of frozen semen, process of freezing and thawing and influential factors of freezing process, aiming to provide a reference for the future semen frozen research.
引文
[1] 孟克格日乐,柴局,张家新,等.不同离心方法去除精浆对内蒙古白绒山羊精液冷冻保存效果的研究[J].中国农学通报,2011,27(26):15-19.
[2] 马毅,曹斌云,姬云涛.山羊除精浆冷冻精液的研究进展[J].中国畜牧兽医,2001,28(4):33-35.
[3] PURDY P H.A review on goat sperm cryopreservation[J].Small Ruminant Research ,2006,63(3):215-225.
[4] Kü?üK N,AKSOY M,U?AN U,et al.Comparison of two different cryopreservation protocols for freezing goat semen.[J].Cryobiology,2014,68(3):327-331.
[5] AZERêDO G A,ESPER C R,RESENDE K T.Evaluation of plasma membrane integrity of frozen-thawed goat spermatozoa with or without seminal plasma[J].Small Ruminant Research,2001,41(3):257-263.
[6] 蔡缪荧,程国虎,张昊.山羊精液颗粒冷冻方法的研究[J].上海畜牧兽医通讯,2015(6):5-6.
[7] 刘孝德,姜勋平,陈家振.山羊精液常温稀释液和最佳稀释倍数研究[J].中国草食动物科学,2003,23(5):17-19.
[8] 权富生,张亨德,马会明,等.除去精浆、添加维生素E及水浴平衡对莎能奶山羊精液冷冻效果的影响[J].西北农业学报,2011,20(4):20-25.
[9] SUKHATO P,THONGSODSEANG S,UTHA A,et al.Effects of cooling and warming conditions on post-thawed motility and fertility of cryopreserved buffalo spermatozoa[J].Animal Reproduction Science,2001,67(1-2):69-77.
[10] 于永生,常万存,李鹏,等.牛卵泡卵母细胞冷冻保存的研究[J].西北农林科技大学学报(自然科学版),2002,30(2):51-54.
[11] 卜书海,孙志杰,李青旺,等.不同解冻方法对奶牛冻精活力的影响[J].西北农林科技大学学报(自然科学版),2002,30(5):40-43.
[12] 权国波,洪琼花.山羊精液冷冻保存研究现状、存在问题及展望[J].中国草食动物科学,2012(S1):26-29.
[13] 杨素芳,邹知明,黎江,等.山羊精液冷冻保存技术研究[J].安徽农业科学,2008,36(11):4 532-4 533.
[14] 赵本田,许常龙.葡萄糖、果糖、聚乙烯醇及牛磺酸对山羊精液保存的影响[J].黑龙江动物繁殖,2007,15(5):9-10.
[15] HU J H,LI Q W,JIANG Z L,et al.The cryoprotective effect of trehalose supplementation on boar spermatozoa quality[J].Reproduction in Domestic Animals,2009,44(4):107-118.
[16] ABOAGLA E M,TERADA T.Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing[J].Biology of Reproduction,2001,69(4):1 245-1 250.
[17] 柴局.内蒙古绒山羊精液冷冻保存的研究[D].呼和浩特:内蒙古农业大学,2011.
[18] 徐振军,车世德,赵冰,等.绒山羊精液低温保存技术研究[J].黑龙江畜牧兽医,2009(19):29-30.
[16] SALAMON S,MAXWELL W M.Storage of ram semen[J].Animal Reproduction Science,2000,62(1-3):77-111.
[20] ARI U ?,KULAKSIZ R,?ZTüRKLER Y.Freezability of tushin ram semen extended with goat or cow milk based extenders[J].Reproduction in Domestic Animals,2011,46(6):975-979.
[21] 杨凌,桑润滋,张会文.羊精液冷冻保存技术研究进展[J].中国草食动物科学,2004,24(1):49-51.
[22] FOROUZANFAR M,SHARAFI M,HOSSEINI S M,et al.In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen[J].Theriogenology,2010,73(4):480.
[23] 鲜红,郑鸿培.猪精液常温保存研究进展[J].黑龙江动物繁殖,2004,12(1):16-19.
[24] Deka B B,Rao A R.Effect of extenders and thawing methods on post thawing preservation of goat semen[J].Indian Veterinary Journal,1987,64(7):591-594.
[25] 刘玉峰,李武.乙二醇对绵羊冻精效果的研究[J].中国草食动物科学,2002,22(6):9-11.
[26] 席利萌,罗军,杨地坤,等.渗透性冷冻保护剂对山羊精子的冷冻保护效果[J].西北农林科技大学学报(自然科学版),2015,43(8):27-32.
[27] 贾永宏,朱庆超,杨海涛,等.牛精液冷冻保护剂研究进展[J].家畜生态学报,2014,35(1):5-8.
[28] KUNDU C,DAS K,MAJUMDER G.Effect of amino acids on goat cauda epididymal sperm cryopreservation using a chemically defined model system[J].Cryobiology,2001,42:21-27.
[29] SIKARWAR A K S,RANJAN R,RAMACHANDRAN N,et al.Comparative effects of different levels of cryoprotectants on freezability of buck semen[J].Indian Journal of Small Ruminants,2016,22(1):110.
[30] KUNDU C N,CHAKRABORTY J,DUTTA P,et al.Development of a simple sperm cryopreservation model using a chemically defined medium and goat cauda epididymal spermatozoa[J].Cryobiology,2000,40(2):117-125.
[31] BüYüKLEBLEBICI S,TUNCER P B,TASDEMIR U,et al.The comparison of three different cryoprotectants in cryopreservation of Angora goat semen[J].Kafkas Uüniversitesi Veteriner Fakuültesi Dergisi,2014,20(4):613-619.
[32] 茆达干,杨利国,吴结革.影响波尔山羊精液低温保存效果的因素[J].中国草食动物,2002(S1):109-111.
[33] 胡日查,郑云胜.内蒙古绒山羊精液冷冻过程中精子活力、活率及顶体变化的探讨[J].中国畜牧兽医,2010,37(5):155-156.
[2] 马毅,曹斌云,姬云涛.山羊除精浆冷冻精液的研究进展[J].中国畜牧兽医,2001,28(4):33-35.
[3] PURDY P H.A review on goat sperm cryopreservation[J].Small Ruminant Research ,2006,63(3):215-225.
[4] Kü?üK N,AKSOY M,U?AN U,et al.Comparison of two different cryopreservation protocols for freezing goat semen.[J].Cryobiology,2014,68(3):327-331.
[5] AZERêDO G A,ESPER C R,RESENDE K T.Evaluation of plasma membrane integrity of frozen-thawed goat spermatozoa with or without seminal plasma[J].Small Ruminant Research,2001,41(3):257-263.
[6] 蔡缪荧,程国虎,张昊.山羊精液颗粒冷冻方法的研究[J].上海畜牧兽医通讯,2015(6):5-6.
[7] 刘孝德,姜勋平,陈家振.山羊精液常温稀释液和最佳稀释倍数研究[J].中国草食动物科学,2003,23(5):17-19.
[8] 权富生,张亨德,马会明,等.除去精浆、添加维生素E及水浴平衡对莎能奶山羊精液冷冻效果的影响[J].西北农业学报,2011,20(4):20-25.
[9] SUKHATO P,THONGSODSEANG S,UTHA A,et al.Effects of cooling and warming conditions on post-thawed motility and fertility of cryopreserved buffalo spermatozoa[J].Animal Reproduction Science,2001,67(1-2):69-77.
[10] 于永生,常万存,李鹏,等.牛卵泡卵母细胞冷冻保存的研究[J].西北农林科技大学学报(自然科学版),2002,30(2):51-54.
[11] 卜书海,孙志杰,李青旺,等.不同解冻方法对奶牛冻精活力的影响[J].西北农林科技大学学报(自然科学版),2002,30(5):40-43.
[12] 权国波,洪琼花.山羊精液冷冻保存研究现状、存在问题及展望[J].中国草食动物科学,2012(S1):26-29.
[13] 杨素芳,邹知明,黎江,等.山羊精液冷冻保存技术研究[J].安徽农业科学,2008,36(11):4 532-4 533.
[14] 赵本田,许常龙.葡萄糖、果糖、聚乙烯醇及牛磺酸对山羊精液保存的影响[J].黑龙江动物繁殖,2007,15(5):9-10.
[15] HU J H,LI Q W,JIANG Z L,et al.The cryoprotective effect of trehalose supplementation on boar spermatozoa quality[J].Reproduction in Domestic Animals,2009,44(4):107-118.
[16] ABOAGLA E M,TERADA T.Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing[J].Biology of Reproduction,2001,69(4):1 245-1 250.
[17] 柴局.内蒙古绒山羊精液冷冻保存的研究[D].呼和浩特:内蒙古农业大学,2011.
[18] 徐振军,车世德,赵冰,等.绒山羊精液低温保存技术研究[J].黑龙江畜牧兽医,2009(19):29-30.
[16] SALAMON S,MAXWELL W M.Storage of ram semen[J].Animal Reproduction Science,2000,62(1-3):77-111.
[20] ARI U ?,KULAKSIZ R,?ZTüRKLER Y.Freezability of tushin ram semen extended with goat or cow milk based extenders[J].Reproduction in Domestic Animals,2011,46(6):975-979.
[21] 杨凌,桑润滋,张会文.羊精液冷冻保存技术研究进展[J].中国草食动物科学,2004,24(1):49-51.
[22] FOROUZANFAR M,SHARAFI M,HOSSEINI S M,et al.In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen[J].Theriogenology,2010,73(4):480.
[23] 鲜红,郑鸿培.猪精液常温保存研究进展[J].黑龙江动物繁殖,2004,12(1):16-19.
[24] Deka B B,Rao A R.Effect of extenders and thawing methods on post thawing preservation of goat semen[J].Indian Veterinary Journal,1987,64(7):591-594.
[25] 刘玉峰,李武.乙二醇对绵羊冻精效果的研究[J].中国草食动物科学,2002,22(6):9-11.
[26] 席利萌,罗军,杨地坤,等.渗透性冷冻保护剂对山羊精子的冷冻保护效果[J].西北农林科技大学学报(自然科学版),2015,43(8):27-32.
[27] 贾永宏,朱庆超,杨海涛,等.牛精液冷冻保护剂研究进展[J].家畜生态学报,2014,35(1):5-8.
[28] KUNDU C,DAS K,MAJUMDER G.Effect of amino acids on goat cauda epididymal sperm cryopreservation using a chemically defined model system[J].Cryobiology,2001,42:21-27.
[29] SIKARWAR A K S,RANJAN R,RAMACHANDRAN N,et al.Comparative effects of different levels of cryoprotectants on freezability of buck semen[J].Indian Journal of Small Ruminants,2016,22(1):110.
[30] KUNDU C N,CHAKRABORTY J,DUTTA P,et al.Development of a simple sperm cryopreservation model using a chemically defined medium and goat cauda epididymal spermatozoa[J].Cryobiology,2000,40(2):117-125.
[31] BüYüKLEBLEBICI S,TUNCER P B,TASDEMIR U,et al.The comparison of three different cryoprotectants in cryopreservation of Angora goat semen[J].Kafkas Uüniversitesi Veteriner Fakuültesi Dergisi,2014,20(4):613-619.
[32] 茆达干,杨利国,吴结革.影响波尔山羊精液低温保存效果的因素[J].中国草食动物,2002(S1):109-111.
[33] 胡日查,郑云胜.内蒙古绒山羊精液冷冻过程中精子活力、活率及顶体变化的探讨[J].中国畜牧兽医,2010,37(5):155-156.