籼粳稻杂交衍生RIL系的苗期抗旱性评价
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摘要
为鉴定籼粳稻杂交衍生系的苗期抗旱性,以课题组自育的高代抗逆品系ZD15为母本、籼稻品种IR29为父本,以及杂交衍生的重组自交系群体120份为试验材料,利用PEG-6000对各材料苗期进行干旱胁迫处理,测定根长、根冠比、地上部鲜重、地下部鲜重、地上部干重和地下部干重;利用PEG-6000对各材料芽期进行干旱胁迫处理,测定芽鞘长和芽长。采用主成分分析和隶属函数法对各材料的抗旱性进行综合评价,根据综合抗旱D值可将122份材料分成3类,D值在0.201～0.400之间的有33份,属于不抗旱材料;D值在0.401～0.600之间的有79份,属于中等抗旱材料;D值在0.601～0.800之间的有10份,属于抗旱材料。利用D值进行逐步回归分析,结果表明根长、根冠比、地上部鲜重、地下部鲜重、地上部干重、地下部干重、芽鞘长和芽长8个性状均可作为水稻苗期抗旱性的评价指标。本研究筛选出的抗旱材料,可作为育种中间材料进一步培育,或作为育种资源加以利用,以丰富本区水稻育种的资源库。
This study attempted to identify the seedling drought resistance of RIL population from an indica and japonica crossing lines.A population of 120 recombinant inbred lines(RIL),which were derived from a cross between an elite indica cultivar IR29 and a drought-resistant japonica line ZD15,were used.An arti?cial drought stress was mimicked by PEG-6000 treatment.These parameters including the germ length and coleoptile length at germination stage,as well as the root length,root-shoot ratio,shoot fresh weight,root fresh weight,shoot dry weight and root dry weight at seedling stage,were measured.With the principal component analysis and membership function method,an objective comprehensive evaluation value(D value)of drought resistance was obtained.According to the D value,the122 rice lines(or variety)were divided into three groups.The group Ⅰ containing 33 lines with D value between 0.201 and 0.400,was a drought sensitive group.The group Ⅱ containing 79 lines with D value between 0.401 and 0.600,was a moderate drought resistant group.The last group containing 10 lines with D value between 0.601 and 0.800,was a drought resistant group.Using stepwise regression and principal component analysis,these eight traits were selected to be evaluation indicators for drought resistance at seedling stage of rice.Taken together,the drought resistant lines selected in this study can be further cultivated as breeding intermediate materials,or used as breeding resources to enrich the rice breeding resource in Ningxia.
This study attempted to identify the seedling drought resistance of RIL population from an indica and japonica crossing lines.A population of 120 recombinant inbred lines(RIL),which were derived from a cross between an elite indica cultivar IR29 and a drought-resistant japonica line ZD15,were used.An arti?cial drought stress was mimicked by PEG-6000 treatment.These parameters including the germ length and coleoptile length at germination stage,as well as the root length,root-shoot ratio,shoot fresh weight,root fresh weight,shoot dry weight and root dry weight at seedling stage,were measured.With the principal component analysis and membership function method,an objective comprehensive evaluation value(D value)of drought resistance was obtained.According to the D value,the122 rice lines(or variety)were divided into three groups.The group Ⅰ containing 33 lines with D value between 0.201 and 0.400,was a drought sensitive group.The group Ⅱ containing 79 lines with D value between 0.401 and 0.600,was a moderate drought resistant group.The last group containing 10 lines with D value between 0.601 and 0.800,was a drought resistant group.Using stepwise regression and principal component analysis,these eight traits were selected to be evaluation indicators for drought resistance at seedling stage of rice.Taken together,the drought resistant lines selected in this study can be further cultivated as breeding intermediate materials,or used as breeding resources to enrich the rice breeding resource in Ningxia.
引文
[1]田又升,谢宗铭,吴向东,王志军,叶春秀,张国丽·水稻种质资源萌发期抗旱性综合鉴定·干旱地区农业研究,2015,33(4):173-180Tian Y S,Xie Z M, Wu X D, Wang Z J, Ye C X, Zhang G L-Identification of drought tolerance of rice germplasm during germination period-Agricultural Research in the Arid Areas,2015,33(4):173-180
[2]Munasinghe M, Adam H P-Genetic and root phenotype diversity in Sri Lankan rice iandraces may be related to drought resistance-Munasinghe and Price Rice, 2016, 9(24):1-13
[3]翟伟,胡小荣,周红立,陶梅·旱稻的抗旱性及遗传改良研究现状·植物遗传资源学报,2010, 11(4):394-398Zhai W, Hu X R, Zhou H L, Tao M.Research status on drought resistance and genetic improvement in upland rice-Journal of Plant Genetic Resources, 2010, 11(4):394-398
[4]Nguyen T T T,Klueva N,Chamareck V,Aarti A,Magpantay G,Millena A C M, Pathan M S, Nguyen H T-Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice-Molecular Genetics and Genomics,2004,272:35-46
[5]Gomez S M, Boopathi N M, Kumar S S, Ramasubramanian T, Zhu C S, Jeyaprakash P, Senthil A, Babu R C-Molecular mapping and location of QTLs for drought-resistance traits in indica rice(Oryza sativa L.)lines adapted to target environments-Acta Physiologiae Plantarum, 2010, 32:355-364
[6]杨瑰丽,杨美娜,李帅良,曲志恒,黄明,陈志强,王慧·水稻萌芽期抗旱指标筛选与抗旱性综合评价·华南农业大学学报,2015,36(2):1-5Yang G L,Yang M N,Li S L,Qu Z H,Huang M,Chen Z Q,Wang H.Screening and comprehensive evaluation of drought resistance indices of rice at germination stage-Journal of South China Agricultural University, 2015,36(2):1-5
[7]Goswam A, Banerjee R, Raha S.Drought resistance in rice seedlings conferred by seed priming-Protoplasma, 2013,250:1115-1129
[8]张芳,颜安,任毅,杨卫君,耿洪伟·新疆冬小麦萌发期抗旱性综合评价·植物遗传资源学报,2019, 20(1):100-112Zhang F, Yan A, Ren Y, Yang W J, Geng H W.Evaluation on drought resistance of winter wheat cultivars in Xinjiang-Joumal of Plant Genetic Resources, 2019,20(1):100-112
[9]胡标林,扬平,万勇,李霞,罗世友,罗向东,谢建坤·东乡野生稻BILs群体苗期抗旱性综合评价及其遗传分析·植物遗传资源学报,2013, 14(2):249-256Hu B L,Yang P,Wan Y,Li X,Luo S Y,Luo X D,Xie J K-Comprehensive assessment of drought resistance of BILs population derived from dongxiang wild rice(Oryza rufipogon Griff.)at seedling stage and its genetic analysis-Journal of Plant Genetic Resources, 2013, 14(2):249-256
[10] Zhang F T,Cui F L,Zhang L X,Wen X F,Luo X D,Zhou Y,Li X, Wan Y, Zhang J, Xie J K-Development and identification of a introgression line with strong drought resistance at seedling stage derived from Oryza sativa L-mating with Oryza rufipogon Griff.Euphytica, 2014, 200:1-7
[11]张洪程,张军,龚金龙,常勇,李敏,高辉,戴其根,霍中洋,许轲,魏海燕·“籼改粳”的生产优势及其形成机理·中国农业科学,2013,46(4):686-704Zhang H C,Zhang J,Gong J L,Chang Y,Li M,Gao H,Dai Q G, Huo Z Y, Xu K, Wei H Y.The productive advantages and formation mechanisms of"Indica rice to japonica rice"-Scientia Agricultura Sinica, 2013,46(4):686-704
[12]孙惠敏,夏米,陈清玲,张凯歌,司二杰,郑卓·基于InDel分子标记的20个水稻品种的籼粳属性分析·井冈山大学学报:自然科学版,2016, 37(6):100-106Sun H M,Xia M,Chen Q L,Zhang K G,Si E J,Zheng Z.Indica-japonica composition analysis of 20 rice varieties base on indel molecular markers-Journal of Jinggangshan University:Natural Science, 2016, 37(6):100-106
[13] Jain A, Balaravi P, Shenoy V.Assessment of yield based selection under managed field stress condition for breeding for rice yield improvement under drought.Biologia,2013,68(4):569-576
[14] Lee H, Cha J, Choi C, Choi N, Ji H S, Park S R, Lee S,Hwang D J-Rice WRKY11 plays a role in pathogen defense and drought tolerance-Rice, 2018, 11(5):1-12
[15] Li N,Kong L,Zhou W H,Zhang X,Wei S T,Ding X H,Chu Z H-Overexpression of Os2H16 enhances resistance to phytopathogens and tolerance to drought stress in rice-Plant Cell Tiss Organ Cult,2013,115:429-441
[16] Chintakovid N,Maipoka M,Phaonakrop N,Mickelbart M V,Roytrakul S, Chadchawan S-Proteomic analysis of droughtresponsive proteins in rice reveals photosynthesis-related adaptations to drought stress-Acta Physiologiae Plantarum,2017, 39(240):1-13
[17] Fang Y J, Xiong L Z.General mechanisms of drought response and their application in drought resistance improvement in plants-Cellular and Molecular Life Sciences, 2015,72:673-689
[18] Li X M,Han H P,Chen M,Yang W,Liu L,Li N,Ding X H,Chu Z H-Overexpression of OsDT11, which encodes a novel cysteine-rich peptide, enhances drought tolerance and increases ABA concentration in rice-Plant Molecular Biologry, 2017,93:21-34
[19] Xiang D J, Man L L,EhEml, a novel Em-like protein from Eutrema halophilum, confers tolerance to salt and drought stresses in rice-Molecular Breeding 2018,38(17):1-15
[20]Joo J,Choi H J,Lee Y H,Kin Y K,Song S I.A transcriptional repressor of the ERF family confers drought tolerance to rice and regulates genes preferentially located on chromosome11-Planta, 2013,238:155-170
[21]孟宪梅,黄义德,李奕松,周可金,武立权,黄木易·水稻若干生理指标与品种抗旱性关系的研究.安徽农业大学学报,2003,30(1):15-22Meng X M,Huang Y D,Li Y S,Zhou K J,Wu L Q,Huang M Y-Studies on some physiological indices and drought resistance among different rice cultivars-Journal of Anhui Agricultural University, 2003,30(1):15-22
[22]Kanbar A,Shashidhar H E.Participatory selection assisted by DNA markers for enhanced drought resistance and productivity in rice(Oryza sativa L.)-Euphytica, 2011, 178:137-150
[23] Yu Y W,Yang D X,Zhou S R,Gu J T,Wang F R,Dong J G,Huang R F-The ethylene response factor OsERF109 negatively affects ethylene biosynthesis and drought tolerance in rice.Protoplasma, 2017, 254:401-408
[24] Wang L L,Yu C C,Chen C,He C L,Zhu Y G,Huang W C.Identification of rice Di19 family reveals OsDil9-4 involved in drought resistance.Plant Cell Reports,2014, 33:2047-2062
[25] Hadiarto T T, Tran L S P-Progress studies of drought-responsive genes in rice-Plant Cell Reports, 2011,30:297-310
[2]Munasinghe M, Adam H P-Genetic and root phenotype diversity in Sri Lankan rice iandraces may be related to drought resistance-Munasinghe and Price Rice, 2016, 9(24):1-13
[3]翟伟,胡小荣,周红立,陶梅·旱稻的抗旱性及遗传改良研究现状·植物遗传资源学报,2010, 11(4):394-398Zhai W, Hu X R, Zhou H L, Tao M.Research status on drought resistance and genetic improvement in upland rice-Journal of Plant Genetic Resources, 2010, 11(4):394-398
[4]Nguyen T T T,Klueva N,Chamareck V,Aarti A,Magpantay G,Millena A C M, Pathan M S, Nguyen H T-Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice-Molecular Genetics and Genomics,2004,272:35-46
[5]Gomez S M, Boopathi N M, Kumar S S, Ramasubramanian T, Zhu C S, Jeyaprakash P, Senthil A, Babu R C-Molecular mapping and location of QTLs for drought-resistance traits in indica rice(Oryza sativa L.)lines adapted to target environments-Acta Physiologiae Plantarum, 2010, 32:355-364
[6]杨瑰丽,杨美娜,李帅良,曲志恒,黄明,陈志强,王慧·水稻萌芽期抗旱指标筛选与抗旱性综合评价·华南农业大学学报,2015,36(2):1-5Yang G L,Yang M N,Li S L,Qu Z H,Huang M,Chen Z Q,Wang H.Screening and comprehensive evaluation of drought resistance indices of rice at germination stage-Journal of South China Agricultural University, 2015,36(2):1-5
[7]Goswam A, Banerjee R, Raha S.Drought resistance in rice seedlings conferred by seed priming-Protoplasma, 2013,250:1115-1129
[8]张芳,颜安,任毅,杨卫君,耿洪伟·新疆冬小麦萌发期抗旱性综合评价·植物遗传资源学报,2019, 20(1):100-112Zhang F, Yan A, Ren Y, Yang W J, Geng H W.Evaluation on drought resistance of winter wheat cultivars in Xinjiang-Joumal of Plant Genetic Resources, 2019,20(1):100-112
[9]胡标林,扬平,万勇,李霞,罗世友,罗向东,谢建坤·东乡野生稻BILs群体苗期抗旱性综合评价及其遗传分析·植物遗传资源学报,2013, 14(2):249-256Hu B L,Yang P,Wan Y,Li X,Luo S Y,Luo X D,Xie J K-Comprehensive assessment of drought resistance of BILs population derived from dongxiang wild rice(Oryza rufipogon Griff.)at seedling stage and its genetic analysis-Journal of Plant Genetic Resources, 2013, 14(2):249-256
[10] Zhang F T,Cui F L,Zhang L X,Wen X F,Luo X D,Zhou Y,Li X, Wan Y, Zhang J, Xie J K-Development and identification of a introgression line with strong drought resistance at seedling stage derived from Oryza sativa L-mating with Oryza rufipogon Griff.Euphytica, 2014, 200:1-7
[11]张洪程,张军,龚金龙,常勇,李敏,高辉,戴其根,霍中洋,许轲,魏海燕·“籼改粳”的生产优势及其形成机理·中国农业科学,2013,46(4):686-704Zhang H C,Zhang J,Gong J L,Chang Y,Li M,Gao H,Dai Q G, Huo Z Y, Xu K, Wei H Y.The productive advantages and formation mechanisms of"Indica rice to japonica rice"-Scientia Agricultura Sinica, 2013,46(4):686-704
[12]孙惠敏,夏米,陈清玲,张凯歌,司二杰,郑卓·基于InDel分子标记的20个水稻品种的籼粳属性分析·井冈山大学学报:自然科学版,2016, 37(6):100-106Sun H M,Xia M,Chen Q L,Zhang K G,Si E J,Zheng Z.Indica-japonica composition analysis of 20 rice varieties base on indel molecular markers-Journal of Jinggangshan University:Natural Science, 2016, 37(6):100-106
[13] Jain A, Balaravi P, Shenoy V.Assessment of yield based selection under managed field stress condition for breeding for rice yield improvement under drought.Biologia,2013,68(4):569-576
[14] Lee H, Cha J, Choi C, Choi N, Ji H S, Park S R, Lee S,Hwang D J-Rice WRKY11 plays a role in pathogen defense and drought tolerance-Rice, 2018, 11(5):1-12
[15] Li N,Kong L,Zhou W H,Zhang X,Wei S T,Ding X H,Chu Z H-Overexpression of Os2H16 enhances resistance to phytopathogens and tolerance to drought stress in rice-Plant Cell Tiss Organ Cult,2013,115:429-441
[16] Chintakovid N,Maipoka M,Phaonakrop N,Mickelbart M V,Roytrakul S, Chadchawan S-Proteomic analysis of droughtresponsive proteins in rice reveals photosynthesis-related adaptations to drought stress-Acta Physiologiae Plantarum,2017, 39(240):1-13
[17] Fang Y J, Xiong L Z.General mechanisms of drought response and their application in drought resistance improvement in plants-Cellular and Molecular Life Sciences, 2015,72:673-689
[18] Li X M,Han H P,Chen M,Yang W,Liu L,Li N,Ding X H,Chu Z H-Overexpression of OsDT11, which encodes a novel cysteine-rich peptide, enhances drought tolerance and increases ABA concentration in rice-Plant Molecular Biologry, 2017,93:21-34
[19] Xiang D J, Man L L,EhEml, a novel Em-like protein from Eutrema halophilum, confers tolerance to salt and drought stresses in rice-Molecular Breeding 2018,38(17):1-15
[20]Joo J,Choi H J,Lee Y H,Kin Y K,Song S I.A transcriptional repressor of the ERF family confers drought tolerance to rice and regulates genes preferentially located on chromosome11-Planta, 2013,238:155-170
[21]孟宪梅,黄义德,李奕松,周可金,武立权,黄木易·水稻若干生理指标与品种抗旱性关系的研究.安徽农业大学学报,2003,30(1):15-22Meng X M,Huang Y D,Li Y S,Zhou K J,Wu L Q,Huang M Y-Studies on some physiological indices and drought resistance among different rice cultivars-Journal of Anhui Agricultural University, 2003,30(1):15-22
[22]Kanbar A,Shashidhar H E.Participatory selection assisted by DNA markers for enhanced drought resistance and productivity in rice(Oryza sativa L.)-Euphytica, 2011, 178:137-150
[23] Yu Y W,Yang D X,Zhou S R,Gu J T,Wang F R,Dong J G,Huang R F-The ethylene response factor OsERF109 negatively affects ethylene biosynthesis and drought tolerance in rice.Protoplasma, 2017, 254:401-408
[24] Wang L L,Yu C C,Chen C,He C L,Zhu Y G,Huang W C.Identification of rice Di19 family reveals OsDil9-4 involved in drought resistance.Plant Cell Reports,2014, 33:2047-2062
[25] Hadiarto T T, Tran L S P-Progress studies of drought-responsive genes in rice-Plant Cell Reports, 2011,30:297-310