基于高通量测序对塔克拉玛干沙漠南缘2种多年生植物根际细菌及真菌群落结构的研究
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摘要
本研究利用高通量测序法(Miseq测序平台)对塔克拉玛干沙漠南缘2种多年生植物根际土壤微生物进行初步研究。结果表明,骆驼刺根际土壤中细菌分为16门、33纲、67目、129科、320属,柽柳根际土壤中细菌分为14门、30纲、66目、125科、335属;骆驼刺根际土壤中真菌分为8门、13纲、36目、58科、104属,柽柳根际土壤中真菌分为8门、13纲、34目、59科、92属。2种植物根际微生物群落结构种间存在差异,真菌差异大于细菌且丰度高。
引文
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[3]SKUJINS J.Microbial ecology of desert soils[J].Advances in Microbial Ecology,1984,7:49-91.下转第页
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[6]LIU J,SUI Y,YU Z,et al.Soil carbon content drives the biogeographical distribution of fungal communities in the black soil zone of northest China[J].Soil Biology&Biochemistry,2015,83(1):29-39.
[7]SHAMIR I,STEINBERGER Y.Vertical distribution and activity of soil microbial population in a sandy desert ecosystem[J].Microbial Ecology,2007,53(2):340-347.
[8]朱军涛,李向义,张希明,等.灌溉对疏叶骆驼刺幼苗光合生理指标及渗透物质的影响[J].中国沙漠,2009,29(4):697-702.
[9]曾凡江,张希明,李小明.骆驼刺植被及其资源保护与开发的意义[J].干旱区地理,2002,25(3):286-288.
[10]李君山,刘勇民,蔡少青.维吾尔医用骆驼刺类药材的资源、商品流通及民间应用研究[J].中国民族医药杂志,1996,2(2):39-40.
[11]张媛,屠鹏飞.柽柳属药用植物研究进展[J].中草药,2008,39(6):947-951.
[12]曾杰,曾凡江,郭海峰,等.策勒绿洲外围2种植物幼苗对NaCl的生理响应[J].干旱区研究,2008,25(5):673-678.
[13]郭海峰,曾凡江,S K ARNDT,等.洪水灌溉对策勒绿洲优势植物及生境的影响[J].科学通报,2008(53):140-146.
[14]张利刚,曾凡江,袁娜,等.不同水分条件下疏叶骆驼刺(Alhagi sparsifolia)生长季根系分株构型特征[J].中国沙漠,2013,33(3):717-723.
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[16]CHAKRAVORTY S,HELB D,BURDAY M,et al.A detailed analysis of16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria[J].Journal of Microbiological Methods,2007,69(2):330-339.
[17]WAKELIN S A,COLLOFF M J,HARVEY P R,et al.The effects of stubble retention and nitrogen application on soil microbial community structure and functional gene abundance under irrigated maize[J].FEMSMicrobiol.Ecol.,2007,59(3):661-670.!!!!!!!!!!!!!!!!!!!!!!!