柳叶拟沉香中倍半萜类化合物的研究
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摘要
通过各种色谱技术,对柳叶拟沉香中的化学成分进行分离,并根据波谱数据结合理化性质共鉴定了2个倍半萜类化合物,分别为12-hydroxy-dihydrocyperolone (1)和(rel)-4β,5β,7β-eremophil-9-en-12,8α-olide (2),其中化合物1是新化合物。活性测试结果显示化合物2对人慢性髓原白血病细胞株K562和人肝癌细胞株BEL-7401具有一定的细胞毒活性,其IC_(50)分别为(17.85±0.04),(21.82±0.07)mg·L~(-1)[紫杉醇为阳性对照,IC_(50)分别为(1.97±0.11),(6.31±0.08) mg·L~(-1)]。
Two sesquiterpenes were isolated from the agarwood originating from Gyrinops salicifolia with various chromatographic techniques, and their structures were determined as 12-hydroxy-dihydrocyperolone(1) and(rel)-4β,5β,7β-eremophil-9-en-12,8α-olide(2), through a combined analysis of physicochemical properties and spectroscopic evidence. Compound 1 was a new compound. Compound 2 showed cytotoxicities against K562 and BEL-7401 cell lines, with IC_(50) values of(17.85±0.04) and(21.82±0.07) mg·L~(-1), respectively [taxol as positive control, with IC_(50) values of(1.97±0.11) and(6.31±0.08) mg·L~(-1)].
Two sesquiterpenes were isolated from the agarwood originating from Gyrinops salicifolia with various chromatographic techniques, and their structures were determined as 12-hydroxy-dihydrocyperolone(1) and(rel)-4β,5β,7β-eremophil-9-en-12,8α-olide(2), through a combined analysis of physicochemical properties and spectroscopic evidence. Compound 1 was a new compound. Compound 2 showed cytotoxicities against K562 and BEL-7401 cell lines, with IC_(50) values of(17.85±0.04) and(21.82±0.07) mg·L~(-1), respectively [taxol as positive control, with IC_(50) values of(1.97±0.11) and(6.31±0.08) mg·L~(-1)].
引文
[1] Li W,Cai C H,Guo Z K,et al.Five neweudesmane-type sesquiterpenoids from Chinese agarwood induced by artificial holing [J].Fitoterapia,2015,100:44.
[2] 邝彤东,陈惠琴,李薇,等.人工打洞沉香中1个新的倍半萜[J].中国中药杂志,2017,42(23):4618.
[3] Xiang P,Mei W L,Chen H Q,et al.Four new bi-phenylethylchromones from artificial agarwood [J].Fitoterapia,2017,120:61.
[4] Li W,Cai C H,Dong W H,et al.2-(2-Phenylethyl)chromone derivatives from Chinese agarwood induced by artificial holing[J].Fitoterapia,2014,98:117.
[5] Iwagoe K,Kakae T,Konishi T,et al.Studies on the agalwood (Jinko).Ⅷ:structures of bi-phenylethyl-chromone derivatives [J].Chem Pham Bull,1989,37:124.
[6] 邵杭,梅文莉,李薇,等.柳叶拟沉香所产沉香的化学成分研究[J].天然产物研究与开发,2015,27:2046.
[7] Shao H,Mei W L,Kong F D,et al.Sesquiterpenes of agarwood from Gyrinops salicifolia [J].Fitoterapia,2016,113:182.
[8] 郭丰娟,梅文莉,董文化,等.柳叶拟沉香中乙酰胆碱酯酶的抑制活性成分[J].热带生物学报,2017,8(1):58.
[9] Mosmann T.Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays [J].J Immunol Methods,1983,65:55.
[10] Ohira S,Hasegawa T,Hayashi K,et al.Sesquiterpenoids from Cyperus rotundus [J].Phytochemistry,1998,47(8):1577.
[11] Camargo M J D,Miranda M L D,Kagamida C M,et al.Sesquiterpenods de Ocotea lancifolia (Lauraceae) [J].Quim Nova,2013,36(7):1008.
[12] 方忠莹,杜思雨,潘少斌,等.景东山橙中生物碱类化学成分[J].中国实验方剂学杂志,2018,24(12):52.
[2] 邝彤东,陈惠琴,李薇,等.人工打洞沉香中1个新的倍半萜[J].中国中药杂志,2017,42(23):4618.
[3] Xiang P,Mei W L,Chen H Q,et al.Four new bi-phenylethylchromones from artificial agarwood [J].Fitoterapia,2017,120:61.
[4] Li W,Cai C H,Dong W H,et al.2-(2-Phenylethyl)chromone derivatives from Chinese agarwood induced by artificial holing[J].Fitoterapia,2014,98:117.
[5] Iwagoe K,Kakae T,Konishi T,et al.Studies on the agalwood (Jinko).Ⅷ:structures of bi-phenylethyl-chromone derivatives [J].Chem Pham Bull,1989,37:124.
[6] 邵杭,梅文莉,李薇,等.柳叶拟沉香所产沉香的化学成分研究[J].天然产物研究与开发,2015,27:2046.
[7] Shao H,Mei W L,Kong F D,et al.Sesquiterpenes of agarwood from Gyrinops salicifolia [J].Fitoterapia,2016,113:182.
[8] 郭丰娟,梅文莉,董文化,等.柳叶拟沉香中乙酰胆碱酯酶的抑制活性成分[J].热带生物学报,2017,8(1):58.
[9] Mosmann T.Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays [J].J Immunol Methods,1983,65:55.
[10] Ohira S,Hasegawa T,Hayashi K,et al.Sesquiterpenoids from Cyperus rotundus [J].Phytochemistry,1998,47(8):1577.
[11] Camargo M J D,Miranda M L D,Kagamida C M,et al.Sesquiterpenods de Ocotea lancifolia (Lauraceae) [J].Quim Nova,2013,36(7):1008.
[12] 方忠莹,杜思雨,潘少斌,等.景东山橙中生物碱类化学成分[J].中国实验方剂学杂志,2018,24(12):52.