HPLC法测定养阴镇静丸中五味子醇甲的含量
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摘要
目的构建HPLC法以评测在养阴镇静丸之中五味子醇甲的总量。方法色谱柱即:Agilent HC C18(规格即:250×4.6 mm,5μm);甲醇-水(比重即:58:42)即流动相;监测波长即250 nm。结果五味子醇甲在0.010-0.209μg的范畴之中凸显出优良的线性关联性,R2即0.9999;平均收回率即99.7%,RSD即0.9%。结论这一方式简易、精准,重现性较优,可作评价养阴镇静丸的质控方法。
Objective to evaluate and determine schisandrin content in Nourishing Yin Sedating Pill with HPLC method. Methods chromatographic column was Agilent HC C18(specification: 250 × 4.6 mm, 5μm); methanol-water(specific gravity: 58:42), mobile phase; monitoring wavelength was 250 nm. Results schisandrin showed excellent linear correlation within range of 0.010μg to 0.209μg, R2 was 0.9999, with average recovery rate of 99.7% and RSD of 0.9%. Conclusion the method is simple, accurate and reproducible, and can be quality control method to evaluate Yangyin Nourishing Yin Sedating pills.
Objective to evaluate and determine schisandrin content in Nourishing Yin Sedating Pill with HPLC method. Methods chromatographic column was Agilent HC C18(specification: 250 × 4.6 mm, 5μm); methanol-water(specific gravity: 58:42), mobile phase; monitoring wavelength was 250 nm. Results schisandrin showed excellent linear correlation within range of 0.010μg to 0.209μg, R2 was 0.9999, with average recovery rate of 99.7% and RSD of 0.9%. Conclusion the method is simple, accurate and reproducible, and can be quality control method to evaluate Yangyin Nourishing Yin Sedating pills.
引文
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[11]陈恩瑜,施夏蓉,洪可俊.RP-HPLC法同时测定补肾益脑胶囊中五味子醇甲、五味子甲素和五味子乙素的含量[J].海峡药学,2012,(12):49-51.
[12]古海锋,张小茜,郭洪祝.HPLC法测定复方益肝灵片中五味子醇甲的含量[J].药物分析杂志,2009,(02):49-52.
[2]李立新,申建柯.参芪五味子片联合金水宝胶囊治疗中老年阳气虚弱型失眠疗效观察,[J].广东中医药大学学报,2012,29(3):251-253.
[3]颜清.参芪五味子片治疗失眠症46例疗效观察[J].海南医学2012,23(11):43-44.
[4]郝吉洪,张宝萍,冯国珍,等.五味子联合护理干预治疗中青年压力性失眠的疗效观察[J].中国伤残医学,2014,22(5):229-230.
[5]许月本.五味子的药理作用和临床应用研究进展[J].中医药导报,2015,21(17):104-106.
[6]付善良,陈波,姚守拙.北五味子和南五味子化学成分的比较分析[J].药物分析杂志,2009,29(4):524.
[7]郭润芳,李建伟.HPLC测定更年安片中丹皮酚和五味子醇甲的含量[J].山西中医学院学报,2014,15(1):29-31.
[8]许乾丽,茅向军,许亚玲,等.HPLC法测定石斛夜光丸中五味子醇甲的含量[J].药品分析杂志,2010,30(12):2360-2362.
[9]王丽薇.五味子的化学成分研究[D].浙江大学,2004.
[10]刘晓明,雷蓉,刘永利,等.HPLC同时测定益脑胶囊中五味子醇甲和五味子乙素的含量[J].中国医药科学,2015,(09):52-54.
[11]陈恩瑜,施夏蓉,洪可俊.RP-HPLC法同时测定补肾益脑胶囊中五味子醇甲、五味子甲素和五味子乙素的含量[J].海峡药学,2012,(12):49-51.
[12]古海锋,张小茜,郭洪祝.HPLC法测定复方益肝灵片中五味子醇甲的含量[J].药物分析杂志,2009,(02):49-52.