生物素链霉亲和素联合免疫层析用于快速检测NT-proBNP
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摘要
为提高常规免疫层析技术的检测灵敏度,引入生物素-链霉亲和素系统,以荧光微球作为标记物,建立了一种新型快速定量检测NT-pro BNP的方法.试验结果表明:新型试剂盒在0.02~30μg·L~(-1)范围能对NT-proBNP实现快速检测.在全血检测中,新型试纸条所测NT-pro BNP浓度与雷度快速免疫分析仪定量检测结果具有很好的一致性.新型免疫层析试纸条大大提高了快速检测的准确性,可广泛用于床旁检验(POCTs)等临床诊断阶段.
In order to improve the sensitivity of conventional immunochromatography,a novel rapid and quantitative assay for NT-pro BNP was established by introducing biotin-streptavidin system and using fluorescent microspheres as a marker.The results showed that the new kit could detect NT-pro BNP rapidly in the range of 0.02 ~ 30 μg·L~(-1). In whole blood test,the NT-pro BNP concentration measured by the new test strip was in good agreement with the quantitative detection results of the Radio Meter AQT90 FLEX analyzer. The new immunochromatographic test strip greatly improved the accuracy of rapid test,and may be widely used in clinical diagnosis such as bedside test( POCTs).
In order to improve the sensitivity of conventional immunochromatography,a novel rapid and quantitative assay for NT-pro BNP was established by introducing biotin-streptavidin system and using fluorescent microspheres as a marker.The results showed that the new kit could detect NT-pro BNP rapidly in the range of 0.02 ~ 30 μg·L~(-1). In whole blood test,the NT-pro BNP concentration measured by the new test strip was in good agreement with the quantitative detection results of the Radio Meter AQT90 FLEX analyzer. The new immunochromatographic test strip greatly improved the accuracy of rapid test,and may be widely used in clinical diagnosis such as bedside test( POCTs).
引文
[1]Mc Kie P,Burnett J C Jr.NT-pro BNP:The gold standard biomarker in heart failure[J].J Am Coll Cardiol,2016,68(22):2437-2439.
[2]Zheng Y R,Ye L F,Cen X J,et al.Low NT-pro BNP levels:An early sign for the diagnosis of ischemic heart failure[J].Int J Cardiol,2017,228:666-671.
[3]杨春莉,裘宇容,周芳,等.血清N端前体脑钠肽在心血管病患者的表达及其临床意义[J].南方医科大学学报,2008,28(5):866-869.
[4]张勇法,杨建英.免疫学检测技术在真菌毒素及兽药残留检测中的应用[J].中国饲料,2014(16):33-35.
[5]Sallam K M,Sidkey N M,Abed N N,et al.Lipopolysaccharidestimulated interleukin-6 production for radioimmunoassay[J].J Radioanal Nucl Ch,2017,313(2):445-453.
[6]Marri L,Jansson A M,Christensen C E,et al.An enzymelinked immunosorbent assay for the detection of diacetyl(2,3-butanedione)[J].Anal Biochem,2017,535:12-18.
[7]Liu Q,Liu X P,Wei YP,et al.Electrochemiluminescence immunoassay for the carcinoembryonic antigen using Cd Se:Eu nanocrystals[J].Microchimica Acta,2017,184(5):1353-1360.
[8]Nardo F D,Anfossia L,Giovannolia C,et al.A fluorescent immunochromatographic strip test using Quantum Dots for fumonisins detection[J].Talanta,2016,150:463-468.
[9]王明良,范金芬.(Roche)罗氏全自动电化学发光分析仪的评价[J].医学信息旬刊,2010,23(4):219-220.
[10]孙宏浩,王甜甜,廖天作,等.金磁复合纳米球的制备及其在检测肌红蛋白中的应用[J].中南民族大学学报(自然科学版),2017,36(1):8-12.
[11]Zhang X G,Shu Y G,Gao J,et al.A new method for blood nt-probnp determination based on a near-infrared point of care testing device with high sensitivity and wide scope[J].Biomed Environ Sci,2017,30(6):426-431.
[12]杨林,罗富里,李赟,等.利用生物素-链霉亲和素进行骨髓间充质干细胞的表面标记[J].中国组织工程研究,2016,20(10):1382-1388.
[13]陈瑜,胡爱国.新型生物素标记的苦杏仁苷活性探针的合成[J].合成化学,2015,23(4):346-349.
[2]Zheng Y R,Ye L F,Cen X J,et al.Low NT-pro BNP levels:An early sign for the diagnosis of ischemic heart failure[J].Int J Cardiol,2017,228:666-671.
[3]杨春莉,裘宇容,周芳,等.血清N端前体脑钠肽在心血管病患者的表达及其临床意义[J].南方医科大学学报,2008,28(5):866-869.
[4]张勇法,杨建英.免疫学检测技术在真菌毒素及兽药残留检测中的应用[J].中国饲料,2014(16):33-35.
[5]Sallam K M,Sidkey N M,Abed N N,et al.Lipopolysaccharidestimulated interleukin-6 production for radioimmunoassay[J].J Radioanal Nucl Ch,2017,313(2):445-453.
[6]Marri L,Jansson A M,Christensen C E,et al.An enzymelinked immunosorbent assay for the detection of diacetyl(2,3-butanedione)[J].Anal Biochem,2017,535:12-18.
[7]Liu Q,Liu X P,Wei YP,et al.Electrochemiluminescence immunoassay for the carcinoembryonic antigen using Cd Se:Eu nanocrystals[J].Microchimica Acta,2017,184(5):1353-1360.
[8]Nardo F D,Anfossia L,Giovannolia C,et al.A fluorescent immunochromatographic strip test using Quantum Dots for fumonisins detection[J].Talanta,2016,150:463-468.
[9]王明良,范金芬.(Roche)罗氏全自动电化学发光分析仪的评价[J].医学信息旬刊,2010,23(4):219-220.
[10]孙宏浩,王甜甜,廖天作,等.金磁复合纳米球的制备及其在检测肌红蛋白中的应用[J].中南民族大学学报(自然科学版),2017,36(1):8-12.
[11]Zhang X G,Shu Y G,Gao J,et al.A new method for blood nt-probnp determination based on a near-infrared point of care testing device with high sensitivity and wide scope[J].Biomed Environ Sci,2017,30(6):426-431.
[12]杨林,罗富里,李赟,等.利用生物素-链霉亲和素进行骨髓间充质干细胞的表面标记[J].中国组织工程研究,2016,20(10):1382-1388.
[13]陈瑜,胡爱国.新型生物素标记的苦杏仁苷活性探针的合成[J].合成化学,2015,23(4):346-349.