生物表面活性剂鼠李糖脂研究概况
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摘要
从化学结构、理化性质、生产方法、提取和纯化、主要应用、研究现状等方面综述了鼠李糖脂的研究,指出了鼠李糖脂未来的研究重点,一是通过诱变和基因工程,筛选出产量更高、遗传性更稳定的高产菌株;二是优化发酵工艺,开发出适合规模化生产的发酵工艺;三是拓宽廉价原材料的范围,进一步降低鼠李糖脂的生产成本;四是对鼠李糖脂的结构、理化性质及其构效关系进行更深入的研究,为鼠李糖脂的开发应用创造更广阔的前景。
The paper summarized the research situation of the rhamnolipid from the perspective of its chemical structure, physicochemical properties, production method, extraction and purification, main application and research status. It pointed out that the research emphasis of the rhamnolipid in future. First, the high-producing strain with stable hereditary should be selected by mutation and genetic engineering. Second, new fermentation process was developed for scale production. Third, new low-cost material should be used to reduce production cost of the rhamnolipid. Fourth, the structure, physicochemical properties and structure-activity relationship of the rhamnolipid should be further studied to expand the application field of the rhamnolipid.
The paper summarized the research situation of the rhamnolipid from the perspective of its chemical structure, physicochemical properties, production method, extraction and purification, main application and research status. It pointed out that the research emphasis of the rhamnolipid in future. First, the high-producing strain with stable hereditary should be selected by mutation and genetic engineering. Second, new fermentation process was developed for scale production. Third, new low-cost material should be used to reduce production cost of the rhamnolipid. Fourth, the structure, physicochemical properties and structure-activity relationship of the rhamnolipid should be further studied to expand the application field of the rhamnolipid.
引文
[1]巩志金,车程川,杨革,等.铜绿假单胞菌发酵产鼠李糖脂生物表面活性剂的方式及条件研究[J].中国酿造,2017,36(10):130-134.
[2]王盼盼,李成婕,何张兰.铜绿假单胞菌半固态发酵生产鼠李糖脂[J].食品与发酵工业,2015,41(11):13-17.
[3]吴虹,汪薇,韩双艳.鼠李糖脂生物表面活性剂的研究进展[J].微生物学通报,2007,34(1):148-152.
[4]Banatim,Franzetti A,Gandolfi I,etal.Microbial biosurfactants production,applications and future potential[J].Applied Microbiology and Biotechnology,2010,87(2):427-444.
[5]Haba E,Pinazo A,Jauregui O,etal.Physicochemical characterization and antimicrobial proper ties of rhamnolipids produced by Pseudomonas aeruginosa 47T2NCBIM 40044[J].Biotechnology&Bioengineering,2010,81(3):316-322.
[6]孙超.铜绿假单胞菌NY3产鼠李糖脂的特性及其应用研究[D].西安筑科技大学,2012.
[7]Camilios N D,Meira J A,de Araujo J M,et al.Production of rhamnolipids in solid-state cultivation using a mixture of sugarcane bagasse and corn bran supplemented with glycerol and soybean oil[J].Applied Microbiology and Biote chnology,2011,89(5):1395-1403.
[8]Siegmund,I and F.Wagner,New Method for Detecting Rhamnolipids Excreted by Pseudomonas Species during Growth on Mineral Agar[J].Biotechnology Techniques,1991,5(4):265-268.
[9]杜瑾,郝建安,张晓菁,等.微生物合成鼠李糖脂生物表面活性剂的研究进展[J].化学与生物工程,2015,32(4):5-11.
[10]王江,曹海龙.一株产鼠李糖脂菌株的筛选、鉴定及其产物特性分析[J].生物技术通报,2017,33(04):191-197.
[11]Rooney A P,Ray K J.Isolation and characterization of rhamnolipid-producing bacterial strains from a biodiesel facility[J].Ferns Microbiology Letters,2009,295(1):82-87.
[12]Lee M,Kim M K.Tetragenococcus koreensis sp.nov.a novel rhamnolipid-producing bacterium[J].International Journal of Systematic&Evolutionary Microbiology,2005,55:1409-1413.
[13]Kiran GS,Thajuddin N,Hema TA,etal.Optimization and characterization of rhamnolipid biosurfactant from sponge associated marine fungi Aspergillus sp msfl[J].Desalination and Water Treatment,2010,24(3):257-265.
[14]赵峰.厌氧产鼠李糖脂菌株Pseudomonas aeruginosa SG的特性及原位驱油潜力研究[D].哈尔滨:哈尔滨工业大学,2016.
[15]Thaniyavam J,Chongchin A,Wanitsuksombut N,etal.Biosurfactant production by Pseudomonas aeruginosa A41using palm oil as carbon source[J].The Journal of general and applied microbiology,2006,52(4):215-222.
[16]Da Silva G P,Mack M,Contiero J.Glycerol.A promising and abundant carbon source for industrial microbiology[J].Biotechnology advances,2009,27(1):30-39.
[17]Olvera C,Goldberg J B,Sanchez R,etal.The Pseudomonas aeruginosa algC gene product participates inrhamnolipid biosynthesis[J].FEMS microbiology letters,1999,179(1):85-90.
[18]Maier R M,Soberon-Chavez G.Pseudomonas aeruginosa rhamnolipids:biosynthesis and potential applications[J].Applied Microbiology and Biotechnology,2000,54(5):625-633.
[19]Rahim R,Burrows L L,Monteiro M A,etal.Involvement of the rml locus in core oligosaccharide and polysaccharide assembly in Pseudomonas aeruginosa[J].Microbiology,2000,146(11):2803-2814.
[20]Marchant R,Banat I M,Srivdhya D.Protocols for measuring biosurfactant production in microbial cultures[M].Springer Protocols Handbooks.Springer,2014:1-10.
[21]Sim L,Ward O P.Production and characterization of a biosurfactant isolated from Pseudomonas aeruginosa,UW-1[J].Journal of industrial microbiology and biotechnology,1997,19(4):232-238.
[22]Morikawa M,Hirata Y.A study on the structure-function relationship of lipopeptide biosurfactants[J].Biochimica Et Biophysics Acta-Molecular and Cell Biology of Lipids,2000,1488(3):211-218.
[23]傅海燕,曾光明,袁兴中,等.生物表面活性剂的分离提纯及其发展前最[J].生物学杂志,2003(06):1-4.
[24]M.Heyd,A Kohnert.Development and trends of biosurfactant analysis and purification using rhamnolipids as an exaxnple[J].Analytical and Bioanalytical Chemistry,2008,391(5):1579-1590.
[25]田静,王靖,冀光.鼠李糖脂生物表面活性剂及其纯化方法研究进展[J].化学与生物工程,2010,27,(6):13-16.
[26]程涛.一株高产鼠李糖脂铜绿假单胞菌的鉴定及环境微生物之间的相互拮抗现象的研究[D].舟山:浙江大学,2017.
[27]Saxena S,Pandey A K.Microbial metabolites as eco-friendly agrochemicals for the next millennium[J].Applied Microbiology and Biotechnology,2001,55(4):395-403.
[28]Li F,Zhu L,Wang L,etal.Gene Expression of an Arthrobacter in Surfactant-Enhanced Biodegradation of a Hydrophobic Organic Compound[J].Environmental Science&Technology,2015,49(6):3698-3704.
[29]Shao B,Liu Z,Zhong H,etal.Effects of rhamnolipids on microorganism characteristics and applications in composting:A review[J].Microbiological Research,2017,200(4):33-44.
[30]Ochoa-Loza F J,Artiola J F,Maier R M.Stability constants for the complexation of various metals with a rhamnolipid biosurfactant[J].Journal of Environmental Quality,2001,30(2):479-485.
[31]蓝梓铭,莫创荣,段秋实,等.鼠李糖脂对剩余污泥中铜和镍的去除[J].环境工程学报,2014,8(3):1174-1178.
[32]任晓蕾,张海英,李玉珍.环脂肤类抗生素达托霉素[J].中国新药杂志,2009,18(12):1075-1080.
[33]Stipcevic T,Piljac A,Piljac G.Enhanced healing of full-thickness bum wounds using di-rhamnolipid[J].Bums,2006,32(1):24-26.
[34]Tahzibi A,Kamal F,Assadi M M,etal.Improved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant[J].Iranian Biomedical Journal,2004,8(1):25-31.
[35]王爽.鼠李糖脂高产菌株诱变筛选、遗传改造及关键酶的异源表达[D].济南:山东大学,2013.
[36]陈利娟,吴斌,何冰芳.ARTP诱变选育鼠李糖脂高产菌及鼠李糖脂促进枯草芽孢杆菌产纤维素酶的研究[J].生物技术通报,2015,31(11):195-201.
[37]Reis R S,Rocha S L G,Chapeaurouge D A,etal.Effects of carbon and nitrogen source on the proteome of Pseudomonas aeruginosa PAI during rhamnolipid production[J].Process Biochem,2010,45(9):1504-1510.
[38]Deziel E,Paquette G.Biosurfactant production by a soil Pseudomonas strain growing on polycyclic aromatic hydrocarbons[J].Applied and Environmental Microbiology,1996,62(6):1908-1912.
[39]Choi M H,Xu J,Gutierrez M,etal.Metabolic relationship between polyhydroxyalkanoic acid and rhamnolipid synthesis in Pseudomonas aeruginosa:Comparative13 CNMR analysis of the products in wild-type and mutants[J].Journal of Biotechnol ogy,2011,151(1):30-42.
[40]Pansiripat S,Pornsunthormtawee O.Biosurfactant production by Pseudomonas aeruginosa SP4 using sequencing batch reactors:Effect of oil-to-glucose ratio[J].biochemical engineering journal,2010,49(2):185-191.
[41]Arino S,Marchal R.Identification and production of a rhamnolipidic biosurfactant by a Pseudomonas species[J].Applied Microbiology and Biotechnology,1996,45(2):162-168.
[42]Lee K M,Hwang S H.Rhamnolipid production in batch and fed-batch fermentation using Pseudomonas aeruginosa BYK-2KCTC 18012P[J].Biotechnology and bioprocess engineering,2004,9(4):267-273.
[43]Guerra-Santos L H,Kappeli O.Dependence of Pseudomonas aeruginosa continous culture biosurfactant production on nutritional and environmental factors[J].Applied Microbiology and Biotechnology,1986,24(6):443-448.
[44]Abalos A,Maximo F.Utilization of response surface methodology to optimize the culture media for the production by Pseudomonas aeruginosa AT10[J].Journal of Chemical Technology and Biotechnology,2002,77(7):777-784.
[45]Chen S Y,Wei Y H,Chang J S.Repeated pH-stat fed-batch fermentation for rhamnolipid production with indigenous Pseudomonas aeruginosa S2[J].Applied Microbiology and Biotechnology,2007,76(1)67-74.
[46]Sarachat T,Pornsunthorntawee O,Chavadej S.Purification and concentration of a rhamnolipid biosurfactant produced by Pseudomonas aeruginosa SP4 using foam fractionation[J].Bioresour Technol,2010,101(1):324-330.
[2]王盼盼,李成婕,何张兰.铜绿假单胞菌半固态发酵生产鼠李糖脂[J].食品与发酵工业,2015,41(11):13-17.
[3]吴虹,汪薇,韩双艳.鼠李糖脂生物表面活性剂的研究进展[J].微生物学通报,2007,34(1):148-152.
[4]Banatim,Franzetti A,Gandolfi I,etal.Microbial biosurfactants production,applications and future potential[J].Applied Microbiology and Biotechnology,2010,87(2):427-444.
[5]Haba E,Pinazo A,Jauregui O,etal.Physicochemical characterization and antimicrobial proper ties of rhamnolipids produced by Pseudomonas aeruginosa 47T2NCBIM 40044[J].Biotechnology&Bioengineering,2010,81(3):316-322.
[6]孙超.铜绿假单胞菌NY3产鼠李糖脂的特性及其应用研究[D].西安筑科技大学,2012.
[7]Camilios N D,Meira J A,de Araujo J M,et al.Production of rhamnolipids in solid-state cultivation using a mixture of sugarcane bagasse and corn bran supplemented with glycerol and soybean oil[J].Applied Microbiology and Biote chnology,2011,89(5):1395-1403.
[8]Siegmund,I and F.Wagner,New Method for Detecting Rhamnolipids Excreted by Pseudomonas Species during Growth on Mineral Agar[J].Biotechnology Techniques,1991,5(4):265-268.
[9]杜瑾,郝建安,张晓菁,等.微生物合成鼠李糖脂生物表面活性剂的研究进展[J].化学与生物工程,2015,32(4):5-11.
[10]王江,曹海龙.一株产鼠李糖脂菌株的筛选、鉴定及其产物特性分析[J].生物技术通报,2017,33(04):191-197.
[11]Rooney A P,Ray K J.Isolation and characterization of rhamnolipid-producing bacterial strains from a biodiesel facility[J].Ferns Microbiology Letters,2009,295(1):82-87.
[12]Lee M,Kim M K.Tetragenococcus koreensis sp.nov.a novel rhamnolipid-producing bacterium[J].International Journal of Systematic&Evolutionary Microbiology,2005,55:1409-1413.
[13]Kiran GS,Thajuddin N,Hema TA,etal.Optimization and characterization of rhamnolipid biosurfactant from sponge associated marine fungi Aspergillus sp msfl[J].Desalination and Water Treatment,2010,24(3):257-265.
[14]赵峰.厌氧产鼠李糖脂菌株Pseudomonas aeruginosa SG的特性及原位驱油潜力研究[D].哈尔滨:哈尔滨工业大学,2016.
[15]Thaniyavam J,Chongchin A,Wanitsuksombut N,etal.Biosurfactant production by Pseudomonas aeruginosa A41using palm oil as carbon source[J].The Journal of general and applied microbiology,2006,52(4):215-222.
[16]Da Silva G P,Mack M,Contiero J.Glycerol.A promising and abundant carbon source for industrial microbiology[J].Biotechnology advances,2009,27(1):30-39.
[17]Olvera C,Goldberg J B,Sanchez R,etal.The Pseudomonas aeruginosa algC gene product participates inrhamnolipid biosynthesis[J].FEMS microbiology letters,1999,179(1):85-90.
[18]Maier R M,Soberon-Chavez G.Pseudomonas aeruginosa rhamnolipids:biosynthesis and potential applications[J].Applied Microbiology and Biotechnology,2000,54(5):625-633.
[19]Rahim R,Burrows L L,Monteiro M A,etal.Involvement of the rml locus in core oligosaccharide and polysaccharide assembly in Pseudomonas aeruginosa[J].Microbiology,2000,146(11):2803-2814.
[20]Marchant R,Banat I M,Srivdhya D.Protocols for measuring biosurfactant production in microbial cultures[M].Springer Protocols Handbooks.Springer,2014:1-10.
[21]Sim L,Ward O P.Production and characterization of a biosurfactant isolated from Pseudomonas aeruginosa,UW-1[J].Journal of industrial microbiology and biotechnology,1997,19(4):232-238.
[22]Morikawa M,Hirata Y.A study on the structure-function relationship of lipopeptide biosurfactants[J].Biochimica Et Biophysics Acta-Molecular and Cell Biology of Lipids,2000,1488(3):211-218.
[23]傅海燕,曾光明,袁兴中,等.生物表面活性剂的分离提纯及其发展前最[J].生物学杂志,2003(06):1-4.
[24]M.Heyd,A Kohnert.Development and trends of biosurfactant analysis and purification using rhamnolipids as an exaxnple[J].Analytical and Bioanalytical Chemistry,2008,391(5):1579-1590.
[25]田静,王靖,冀光.鼠李糖脂生物表面活性剂及其纯化方法研究进展[J].化学与生物工程,2010,27,(6):13-16.
[26]程涛.一株高产鼠李糖脂铜绿假单胞菌的鉴定及环境微生物之间的相互拮抗现象的研究[D].舟山:浙江大学,2017.
[27]Saxena S,Pandey A K.Microbial metabolites as eco-friendly agrochemicals for the next millennium[J].Applied Microbiology and Biotechnology,2001,55(4):395-403.
[28]Li F,Zhu L,Wang L,etal.Gene Expression of an Arthrobacter in Surfactant-Enhanced Biodegradation of a Hydrophobic Organic Compound[J].Environmental Science&Technology,2015,49(6):3698-3704.
[29]Shao B,Liu Z,Zhong H,etal.Effects of rhamnolipids on microorganism characteristics and applications in composting:A review[J].Microbiological Research,2017,200(4):33-44.
[30]Ochoa-Loza F J,Artiola J F,Maier R M.Stability constants for the complexation of various metals with a rhamnolipid biosurfactant[J].Journal of Environmental Quality,2001,30(2):479-485.
[31]蓝梓铭,莫创荣,段秋实,等.鼠李糖脂对剩余污泥中铜和镍的去除[J].环境工程学报,2014,8(3):1174-1178.
[32]任晓蕾,张海英,李玉珍.环脂肤类抗生素达托霉素[J].中国新药杂志,2009,18(12):1075-1080.
[33]Stipcevic T,Piljac A,Piljac G.Enhanced healing of full-thickness bum wounds using di-rhamnolipid[J].Bums,2006,32(1):24-26.
[34]Tahzibi A,Kamal F,Assadi M M,etal.Improved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant[J].Iranian Biomedical Journal,2004,8(1):25-31.
[35]王爽.鼠李糖脂高产菌株诱变筛选、遗传改造及关键酶的异源表达[D].济南:山东大学,2013.
[36]陈利娟,吴斌,何冰芳.ARTP诱变选育鼠李糖脂高产菌及鼠李糖脂促进枯草芽孢杆菌产纤维素酶的研究[J].生物技术通报,2015,31(11):195-201.
[37]Reis R S,Rocha S L G,Chapeaurouge D A,etal.Effects of carbon and nitrogen source on the proteome of Pseudomonas aeruginosa PAI during rhamnolipid production[J].Process Biochem,2010,45(9):1504-1510.
[38]Deziel E,Paquette G.Biosurfactant production by a soil Pseudomonas strain growing on polycyclic aromatic hydrocarbons[J].Applied and Environmental Microbiology,1996,62(6):1908-1912.
[39]Choi M H,Xu J,Gutierrez M,etal.Metabolic relationship between polyhydroxyalkanoic acid and rhamnolipid synthesis in Pseudomonas aeruginosa:Comparative13 CNMR analysis of the products in wild-type and mutants[J].Journal of Biotechnol ogy,2011,151(1):30-42.
[40]Pansiripat S,Pornsunthormtawee O.Biosurfactant production by Pseudomonas aeruginosa SP4 using sequencing batch reactors:Effect of oil-to-glucose ratio[J].biochemical engineering journal,2010,49(2):185-191.
[41]Arino S,Marchal R.Identification and production of a rhamnolipidic biosurfactant by a Pseudomonas species[J].Applied Microbiology and Biotechnology,1996,45(2):162-168.
[42]Lee K M,Hwang S H.Rhamnolipid production in batch and fed-batch fermentation using Pseudomonas aeruginosa BYK-2KCTC 18012P[J].Biotechnology and bioprocess engineering,2004,9(4):267-273.
[43]Guerra-Santos L H,Kappeli O.Dependence of Pseudomonas aeruginosa continous culture biosurfactant production on nutritional and environmental factors[J].Applied Microbiology and Biotechnology,1986,24(6):443-448.
[44]Abalos A,Maximo F.Utilization of response surface methodology to optimize the culture media for the production by Pseudomonas aeruginosa AT10[J].Journal of Chemical Technology and Biotechnology,2002,77(7):777-784.
[45]Chen S Y,Wei Y H,Chang J S.Repeated pH-stat fed-batch fermentation for rhamnolipid production with indigenous Pseudomonas aeruginosa S2[J].Applied Microbiology and Biotechnology,2007,76(1)67-74.
[46]Sarachat T,Pornsunthorntawee O,Chavadej S.Purification and concentration of a rhamnolipid biosurfactant produced by Pseudomonas aeruginosa SP4 using foam fractionation[J].Bioresour Technol,2010,101(1):324-330.