白细胞介素4降低脐带间充质干细胞对造血干细胞分化潜能的维持能力
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摘要
目的探讨白细胞介素4(IL-4)对脐带间充质干细胞(UC-MSC)维持造血干细胞分化的影响。方法将UC-MSC与脐带血CD34~+造血干细胞按照造血支持能力常用的方案共培养,实验分为对照组和IL-4组,IL-4处理组加入IL-4(20 ng/ml)培养14 d。收集细胞并计数,使用流式细胞仪检测表达CD34的细胞比例。取3×10~3个细胞,加入到半固体培养基,培养14 d后,通过倒置显微镜观察比较各种集落的形成,并使用流式细胞仪分析其中巨噬细胞和粒细胞表面特异性蛋白CD11b、CD14和CD15的表达。对两独立样本进行t检验统计学分析。结果加入IL-4后,共培养体系中细胞数量(1.31±0.05)×10~5个/孔与对照组(2.80±0.28)×10~5个/孔相比下降,差异有统计学意义(t=7.31,P <0.05),并且流式细胞分析显示其中的CD34~+细胞比例也有降低。3×10~3个IL-4组得到的细胞形成巨噬细胞集落形成单位的能力(9.33±1.53)个/孔较对照组(17.67±0.58)个/孔有明显下降,差异有统计学意义(t=8.84,P <0.001);形成粒-巨噬集落形成单位的能力(15.67±3.22)个/孔较对照组(29.33±4.04)个/孔有明显下降,差异有统计学意义(t=4.58,P <0.05);形成总集落单位的能力(39.33±9.07)个/孔较对照组(62.67±6.66)个/孔也有下降,差异有统计学意义(t=3.59,P <0.05)。IL-4组得到的细胞分化出的细胞总数(3.67±1.71)×10~5个/孔与对照组(9.50±3.13)×10~5个/孔相比也明显下降,差异有统计学意义(t=2.83,P <0.05),而流式细胞术分析发现分化成的细胞中CD14~+细胞比例也下降。结论 IL-4可以降低UC-MSC对造血干细胞分化潜能的维持能力,提示在Ⅱ型辅助T细胞相关体液免疫疾病中使用间充质干细胞治疗时,也需要兼顾机体造血相关功能。
Objective Umbilical cord mesenchymal stem cells(UC-MSC) are a hot research field because of its unique advantages. UC-MSC possess the ability of maintaince the differentiation potential of hematopoietic stem cells, and we studied the effect of interleukin-4 on this ability.Methods Umbilical cord mesenchymal stem cells were co-cultured with cord blood CD34~+ cells with or without IL-4. CD34~+ cells were counted after 14 days. Moreover, colony forming ability of cells was evaluated. CD11 b, CD14 and CD15 expression of cells from colony forming assay was assayed by flow cytometry. Statistical analysis was performed by unpaired t-test. After IL-4 was added, the number of cells(1.31±0.05)×10~5 cells/well decreased significantly compared with the control group(2.80±0.28)×10~5 cells/well(t = 7.31, P < 0.05), and flow cytometry analysis showed that the proportion of CD34~+ cells in the co-culture system also decreased. The ability of IL-4-treated cells to form colony forming unit-macrophage(9.33±1.53 per well)was significantly lower than that of the control group(17.67±0.58 per well, t = 8.84, P < 0.001); The ability to form colony forming unit-granulocyte/macrophage(15.67±3.22 per well)was significantly lower than that of the control group(29.33±4.04 per well, t = 4.58, P < 0.05). The ability to form total colony forming units(39.33±9.07 per well)was also lower than that of the control group(62.67±6.66 per well, t = 3.59, P < 0.05). The total number of cells differentiated from IL-4 group(3.67±1.71)×10~5 cells/well was also significantly lower than that of the control group(9.50±3.13)×10~5 cells/well,(t = 2.83, P < 0.05), and flow cytometry analysis showed that the ratio of CD14~+ cells in differentiated cells was also lower. Conclusion IL-4 can reduce the maintenance ability of umbilical cord mesenchymal stem cells on hematopoietic stem cells differentiation, suggesting that when mesenchymal stem cells are used in treatment of type II helper T cell-related humoral immune diseases, the hematopoietic related functions should also be considered.
Objective Umbilical cord mesenchymal stem cells(UC-MSC) are a hot research field because of its unique advantages. UC-MSC possess the ability of maintaince the differentiation potential of hematopoietic stem cells, and we studied the effect of interleukin-4 on this ability.Methods Umbilical cord mesenchymal stem cells were co-cultured with cord blood CD34~+ cells with or without IL-4. CD34~+ cells were counted after 14 days. Moreover, colony forming ability of cells was evaluated. CD11 b, CD14 and CD15 expression of cells from colony forming assay was assayed by flow cytometry. Statistical analysis was performed by unpaired t-test. After IL-4 was added, the number of cells(1.31±0.05)×10~5 cells/well decreased significantly compared with the control group(2.80±0.28)×10~5 cells/well(t = 7.31, P < 0.05), and flow cytometry analysis showed that the proportion of CD34~+ cells in the co-culture system also decreased. The ability of IL-4-treated cells to form colony forming unit-macrophage(9.33±1.53 per well)was significantly lower than that of the control group(17.67±0.58 per well, t = 8.84, P < 0.001); The ability to form colony forming unit-granulocyte/macrophage(15.67±3.22 per well)was significantly lower than that of the control group(29.33±4.04 per well, t = 4.58, P < 0.05). The ability to form total colony forming units(39.33±9.07 per well)was also lower than that of the control group(62.67±6.66 per well, t = 3.59, P < 0.05). The total number of cells differentiated from IL-4 group(3.67±1.71)×10~5 cells/well was also significantly lower than that of the control group(9.50±3.13)×10~5 cells/well,(t = 2.83, P < 0.05), and flow cytometry analysis showed that the ratio of CD14~+ cells in differentiated cells was also lower. Conclusion IL-4 can reduce the maintenance ability of umbilical cord mesenchymal stem cells on hematopoietic stem cells differentiation, suggesting that when mesenchymal stem cells are used in treatment of type II helper T cell-related humoral immune diseases, the hematopoietic related functions should also be considered.
引文
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12 Deng DQ,Zhang PL,Guo Y,et al.A randomised double-blind,placebocontrolled trial of allogeneic umbilical cord-derived mesenchymal stem cell for lupus nephritis[J].Ann Rheum Dis,2017,76(8):1436-1439.
13 Jang YK,Kim M,Lee YH,et al.Optimization of the therapeutic efficacy of human umbilical cord blood-mesenchymal stromal cells in an NSG mouse xenograft model of graft-versus-host disease[J].Cytotherapy,2014,16(3):298-308.
2 Lu LL,Liu YJ,Yang SG,et al.Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials[J].Haematologica,2006,91(8):1017-1026.
3 Yang ZX,Mao GX,Zhang J,et al.IFN-gamma induces senescencelike characteristics in mouse bone marrow mesenchymal stem cells[J].Adv Clin Exp Med,2017,26(2):201-206.
4 Xu FH,Yi JZ,Wang ZY,et al.IL-27 regulates the adherence,proliferation,and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations[J].Immunol Res,2017,65(4):903-912.
5 Croitoru-Lamoury J,Lamoury FM,Caristo M,et al.Interferon-γregulates the proliferation and differentiation of mesenchymal stem cells via activation of indoleamine 2,3 dioxygenase(IDO)[J].PLoSOne,2011,6(2):e14698.
6 Mohammadpour H,Pourfathollah AA,Zarif MN,et al.TNF-alpha modulates the immunosuppressive effects of MSCs on dendritic cells and T cells[J].Int Immunopharmacol,2015,28(2,SI):1009-1017.
7杨舟鑫,韩之波,贾兵兵,等.白细胞介素4调节人脐带间充质干细胞的增殖和造血支持能力[J].中国病理生理杂志,2014,30(11):2048-2053.
8 Yang ZX,Chi Y,Ji YR,et al.Human umbilical cord mesenchymal stem cells increase interleukin-9 production of CD4(+)T cells[J].Exp Ther Med,2017,14(4):3541-3548.
9 Chen JC,Feng XM,Desierto MJ,et al.IFN-gamma-mediated hematopoietic cell destruction in murine models of immune-mediated bone marrow failure[J].Blood,2015,126(24):2621-2631.
10及月茹,杨舟鑫,李丽娜,等.IL-1β促进脐带间充质干细胞的造血支持作用[J].中国实验血液学杂志,2013,21(4):1005-1009.
11 Resnick IB,Barkats C,Shapira MY,et al.Treatment of severe steroid resistant acute GVHD with mesenchymal stromal cells(MSC)[J].Am JBlood Res,2013,3(3):225-238.
12 Deng DQ,Zhang PL,Guo Y,et al.A randomised double-blind,placebocontrolled trial of allogeneic umbilical cord-derived mesenchymal stem cell for lupus nephritis[J].Ann Rheum Dis,2017,76(8):1436-1439.
13 Jang YK,Kim M,Lee YH,et al.Optimization of the therapeutic efficacy of human umbilical cord blood-mesenchymal stromal cells in an NSG mouse xenograft model of graft-versus-host disease[J].Cytotherapy,2014,16(3):298-308.