纤维蛋白原Bβ链HinfⅠ基因多态性与胃癌的相关性
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摘要
目的:探讨纤维蛋白原B(FgB)β链-HinfⅠA/C基因多态性与胃癌的相关性。方法:151例经病理确诊的胃癌患者为胃癌组,151例同期健康体检者为对照组,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测2组受检查全血DNA FgBβ-HinfⅠ位点基因型,比较两组受粒者FgBβHinfⅠC基因多态性,分析FgBβHinfⅠC基因型分布与胃癌的临床分期及转移的关系,采用logistic回归分析FgBβHinfⅠC基因型与胃癌的相对危险度。结果:FgBβHinfⅠA/C位点AA、AC、CC基因型分布及A/C等位基因频率与正常对照组比较差异具有统计学意义(P<0.05),FgBβHinfⅠA/C位3种基因型分布和等位基因频率与胃癌的临床分期及转移比较,差异无统计学意义(P>0.05);FgBβHinfⅠA/C位点AC基因型与胃癌的相对危险度分析,结果显示,OR=1.888、95%置信区间CI为1.105～3.224。结论:FgBβ-HinfⅠA/C的AC基因型可能增加胃癌的发病风险,C等位基因可能是胃癌发病的致病基因。
Objective: To investigate relevance between Fibrinogen B beta chain-HinfⅠgene polymorphism and gastric cancer. Methods: 151 cases of pathological diagnosed gastric cancer patients as gastric cancer group, and 151 cases of healthy physical check takers as control group; the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technology was adapted to detect Fibrinogen beta chain-HinfⅠ site genotype. Results: Gastric cancer group fibrinogen beta beta-Hinf I A/C locus AA, AC, CC genotype distribution and A/C allele frequency showed significant difference with normal control group(P<0.05); Fibrinogen beta-HinfIA/C locus of three kinds of genotype distribution and allele frequency was compared with clinical staging and transfer of gastric cancer, comparison was statistically significant(P>0.05); analysis between Fibrinogen beta-HinfI A/C locus AC genotype and relative risk degree of gastric cancer showed odds ratio(OR) as 1.888 and 95% confidence interval(CI) between 1.105 and 3.224. Conclusions: FgB beta Hinf Ⅰ AC of the A/C genotype may increase the risk of gastric cancer, C allele may be disease genes of gastric cancer.
Objective: To investigate relevance between Fibrinogen B beta chain-HinfⅠgene polymorphism and gastric cancer. Methods: 151 cases of pathological diagnosed gastric cancer patients as gastric cancer group, and 151 cases of healthy physical check takers as control group; the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technology was adapted to detect Fibrinogen beta chain-HinfⅠ site genotype. Results: Gastric cancer group fibrinogen beta beta-Hinf I A/C locus AA, AC, CC genotype distribution and A/C allele frequency showed significant difference with normal control group(P<0.05); Fibrinogen beta-HinfIA/C locus of three kinds of genotype distribution and allele frequency was compared with clinical staging and transfer of gastric cancer, comparison was statistically significant(P>0.05); analysis between Fibrinogen beta-HinfI A/C locus AC genotype and relative risk degree of gastric cancer showed odds ratio(OR) as 1.888 and 95% confidence interval(CI) between 1.105 and 3.224. Conclusions: FgB beta Hinf Ⅰ AC of the A/C genotype may increase the risk of gastric cancer, C allele may be disease genes of gastric cancer.
引文
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[10]元小冬.白介素和FgBβ-1420G/A、-993C/T、1689T/G、BsmAIG/C、 I6I/D、345C/T、HinfIA/C 基因多态性与血浆Fg浓度及分子功能的相关性研究[J].细胞与分子免疫学杂志,2012,28(2):190-193.
[11]刘红,胡永华.遗传流行病学研究中的 H-W平衡检验[J].中南大学学报(医学版),2010,35(1):90-93.
[12]孙川,梁亮.α和β纤维蛋白原基因核苷酸多态性及其单体型与冠心病的关系[J].中国动脉硬化杂志,2007,15(9):699-702.
[13]徐建辉,元小冬.FgBβ-1689T/G、I6I/D、345C/T和Hinf IA/C基因多态性与血浆Fg功能表达的相关性研究[J].中国免疫学杂志,2011,27(7):634-638.
[2] CHEN W,ZHENG R,BAADE P D,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
[3] SANO T.Gastric cancer:Asia and the world[J].Gastric Cancer,2017,20(Suppl 1):1-2.
[4] 王静文,韩韬.结直肠癌患者β-纤维蛋白原基因多态性和血浆纤维蛋白原水平相关性的研究[J].中国癌症杂志,2015,25(10):807-811.
[5] LONSDOR A S,KRAMER B F,FAHRLEITNER M,et al.Engagement of αⅡbβ3 (GPⅡb/Ⅲa) with ανβ3 integrin mediates interaction of melanoma cells with platelets:aconnection to hematogenous metastasis[J].J Biol Chem,2012,287(3):2168-2178.
[6] ALVESL C S,BURDICK M M,THOMAS S N,et al.The dual role of CD44 as a functional P-selectin ligand and fibrin receptor in colon carcinoma cell adhesion[J].Am J Physiol Cell Physiol,2008,294(4):907-916.
[7] ROY S N,MUKHOPADHYAY G,REDRNAN C M.Regulation of fibrinogen assembly.Transfection of Hep G2 cells with B beta cDNA specifically enhances synthesis of the three component chains of fibrinogen[J].J Biol Chem,1990,265(11):6389-6393.
[8] NEERMAN-ARBEZ M,DE MOERLOOSE P,BRIDEL C,et al.Mutations in the fibrinogen aalpha gene account for the majority of cases of congenital afibrinogenemia[J].Blood,2000,96(1):149-152.
[9] S.E.Humphries,M.Cook.role of genetic variation at the ibrinogen locus in determination oplasma fibrinogen concentrations[J].The Lancet,June27,1987,1452-1455.
[10]元小冬.白介素和FgBβ-1420G/A、-993C/T、1689T/G、BsmAIG/C、 I6I/D、345C/T、HinfIA/C 基因多态性与血浆Fg浓度及分子功能的相关性研究[J].细胞与分子免疫学杂志,2012,28(2):190-193.
[11]刘红,胡永华.遗传流行病学研究中的 H-W平衡检验[J].中南大学学报(医学版),2010,35(1):90-93.
[12]孙川,梁亮.α和β纤维蛋白原基因核苷酸多态性及其单体型与冠心病的关系[J].中国动脉硬化杂志,2007,15(9):699-702.
[13]徐建辉,元小冬.FgBβ-1689T/G、I6I/D、345C/T和Hinf IA/C基因多态性与血浆Fg功能表达的相关性研究[J].中国免疫学杂志,2011,27(7):634-638.