黄芪注射液对人鼻咽癌CNE-1细胞生物学行为的影响
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摘要
目的:探讨黄芪注射液对人鼻咽癌CNE-1细胞生物学行为的影响。方法:将人鼻咽癌CNE-1细胞分为对照组及不同浓度黄芪注射液的实验组,在光学显微镜下观察细胞形态学变化;划痕修复实验、Transwell迁移实验观察细胞迁移能力;Transwell Matrigel侵袭实验观察细胞侵袭能力。结果:显微镜下可见实验组细胞增殖明显受到抑制,生长缓慢。划痕修复实验中,细胞培养12 h及24 h后,不同浓度黄芪注射液的实验组划痕愈合率均明显低于对照组(均P<0.01)。Transwell迁移实验及侵袭实验显示,对照组比较,不同浓度黄芪注射液的实验组迁移细胞数及侵袭细胞数均明显减少(均P<0.05)。结论:黄芪注射液可抑制人鼻咽癌CNE-1细胞增殖,降低人鼻咽癌CNE-1细胞迁移、侵袭能力。
Objective:To evaluate the effect of Astragalus membranaceus injection on the biological behavior of nasopharyngeal carcinoma(NPC) CNE-1 cells.Methods:Human NPC CNE-1 cells were divided into a control group and different concentrations of Astragalus injection group(experimental groups).The changes of cell morphology were observed under light microscope.The cell migration and invasion capabilities were detected by wound healing assay,Transwell migration assay and Transwell Matrigel invasion assay,respectively.Results:The cell proliferation was significantly inhibited in the experimental groups,and the growth of cells was slow.The migration rate of the experimental groups was lower than that of the control group at 12 h and 24 h(P<0.01).The concentration of migrating cells and the number of invading cells were significantly reduced in the experimental groups when compared with the control group(P<0.05).Conclusion:Astragalus membranaceus injection could inhibit the proliferation of NPC CNE-1 cells and reduce the migration and invasion capabilities of CNE-1 cells.
Objective:To evaluate the effect of Astragalus membranaceus injection on the biological behavior of nasopharyngeal carcinoma(NPC) CNE-1 cells.Methods:Human NPC CNE-1 cells were divided into a control group and different concentrations of Astragalus injection group(experimental groups).The changes of cell morphology were observed under light microscope.The cell migration and invasion capabilities were detected by wound healing assay,Transwell migration assay and Transwell Matrigel invasion assay,respectively.Results:The cell proliferation was significantly inhibited in the experimental groups,and the growth of cells was slow.The migration rate of the experimental groups was lower than that of the control group at 12 h and 24 h(P<0.01).The concentration of migrating cells and the number of invading cells were significantly reduced in the experimental groups when compared with the control group(P<0.05).Conclusion:Astragalus membranaceus injection could inhibit the proliferation of NPC CNE-1 cells and reduce the migration and invasion capabilities of CNE-1 cells.
引文
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[6] 陈淑萍,周红桃,蔡俊宏,等.联合基因Survivin、CDKI的shRNAs靶向干扰对鼻咽癌CNE-2细胞增值的影响[J].基因组学与应用生物学,2014,33(2):239-247.
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[10] 陈红红,莫书荣,吴忧.红景天苷与黄芪注射液协同对人肝癌HepG2细胞增殖的抑制作用[J].基因组学与应用生物学,2016,35(9):2241-2245.
[11] 湛达河,胡建兵,达世俭,等.雷公藤红素对鼻咽癌CNE-1细胞增殖抑制和放射增敏作用的初步研究[J].癌症进展,2010,8(5):491-499.
[12] 刘成军,韦世秀,李牡艳,等.黄芪注射液对人鼻咽癌CNE-2细胞株的抑制作用研究[J].中国药房,2005,16(18):1376-1378.
[13] ZHOU R,XU L,YE M,et al.Formononetin inhibits migration and invasion of MDA-MB-231 and 4T1 breast cancer cells by suppressing MMP-2 and MMP-9 through PI3K/AKT signaling pathways[J].Horm Metab Res,2014,46(11):753-760.
[14] AUYEUNG K K,WOO P K,LAW P C,et al.Astragalus saponins modulate cell invasiveness and angiogenesis in human gastric adenocarcinoma cells[J].J Ethnopharmacol,2012,141(2):635-641.
[15] NIE X H,OU-YANG J,XING Y,et al.Calycosin inhibits migration and invasion through modulation of transforming growth factor beta-mediated mesenchymal properties in U87 and U251 cells[J].Drug Des Devel Ther,2016(10):767-779.
[2] SONG Y,YANG J,BAI W L,et al.Antitumor and immunoregulatory effects of astragalus on nasopharyngeal carcinoma in vivo and in vitro[J].Phytother Res,2011,25(6):909-915.
[3] 李海玉,陈兴凤,余思颖,等.抑制Bmi-1基因表达对鼻咽癌细胞生物学行为的影响[J].中国药理学通报,2014,30(6):833-837.
[4] 陈红红,黄芪注射液抗鼻咽癌作用的初步研究[D].南宁:广西医科大学,2017.
[5] 潘宇,王瑞平,凌博凡,等.健脾化瘀方对肝癌HepG2细胞生物行为学的影响[J].辽宁中医药大学学报,2011,13(10):57-59.
[6] 陈淑萍,周红桃,蔡俊宏,等.联合基因Survivin、CDKI的shRNAs靶向干扰对鼻咽癌CNE-2细胞增值的影响[J].基因组学与应用生物学,2014,33(2):239-247.
[7] CHO W C,LEUNG K N.In vitro and in vivo anti-tumor effects of Astragalus membranaceus[J].Cancer Lett,2007,252(1):43-54.
[8] 胡兵,沈克平.黄芪抗肿瘤作用及机制研究[J].中药材,2008,33(3):461-465.
[9] 丁繁,王小虎.中药黄芪抗肿瘤研究进展[J].肿瘤学杂志,2013,19(1):64-68.
[10] 陈红红,莫书荣,吴忧.红景天苷与黄芪注射液协同对人肝癌HepG2细胞增殖的抑制作用[J].基因组学与应用生物学,2016,35(9):2241-2245.
[11] 湛达河,胡建兵,达世俭,等.雷公藤红素对鼻咽癌CNE-1细胞增殖抑制和放射增敏作用的初步研究[J].癌症进展,2010,8(5):491-499.
[12] 刘成军,韦世秀,李牡艳,等.黄芪注射液对人鼻咽癌CNE-2细胞株的抑制作用研究[J].中国药房,2005,16(18):1376-1378.
[13] ZHOU R,XU L,YE M,et al.Formononetin inhibits migration and invasion of MDA-MB-231 and 4T1 breast cancer cells by suppressing MMP-2 and MMP-9 through PI3K/AKT signaling pathways[J].Horm Metab Res,2014,46(11):753-760.
[14] AUYEUNG K K,WOO P K,LAW P C,et al.Astragalus saponins modulate cell invasiveness and angiogenesis in human gastric adenocarcinoma cells[J].J Ethnopharmacol,2012,141(2):635-641.
[15] NIE X H,OU-YANG J,XING Y,et al.Calycosin inhibits migration and invasion through modulation of transforming growth factor beta-mediated mesenchymal properties in U87 and U251 cells[J].Drug Des Devel Ther,2016(10):767-779.