2份野桑蚕材料线粒体ATPsynthase F0 subunit 6基因的克隆及遗传进化分析
摘要
本研究对2份野桑蚕材料(ws-aksq和ws-akhb)线粒体atp6基因进行了克隆和序列分析。克隆结果表明,2株野桑蚕atp6基因ORF框全长678bp,编码225个氨基酸残基。序列联配比对结果表明2株野桑蚕atp6基因之间存在6处碱基的差异。ws-aksq野桑蚕atp6基因与家蚕c108、野桑蚕Qingzhou系、野桑蚕日本系的相似度分别为0.9926、0.9912和0.9690;ws-akhb野桑蚕与上述3者的相似度分别为0.9926、1.0000、0.9676。系统发生分析表明,鳞翅目下7个亚科的昆虫atp6基因分别聚为7枝,2份野桑蚕atp6基因与中国系野桑蚕亲缘关系较为接近,与日本株较远。本研究为深入利用线粒体基因组开展野桑蚕分类及与其它昆虫的遗传进化研究提供了理论基础。
引文
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[2]Xia Q,Guo Y,Zhang Z,Li D,Xuan Z,Li Z,Dai F,Li Y,Cheng D,Li R,Cheng T,Jiang T,Becquet C,Xu X,Liu C,Zha X,Fan W,Lin Y,Shen Y,Jiang L,Jensen J,Hellmann I,Tang S,Zhao P,Xu H,Yu C,Zhang G,Li J,Cao J,Liu S,He N,Zhou Y,Liu H,Zhao J,Ye C,Du Z,Pan G,Zhao A,Shao H,Zeng W,Wu P,Li C,Pan M,Li J,Yin X,Li D,Wang J,Zheng H,Wang W,Zhang X,Li S,Yang H,Lu C,Nielsen R,Zhou Z,Wang J,Xiang Z,Wang J.Complete resequencing of 40 genomes reveals domestication events and genes in silkworm(Bombyx)[J].Science,2009,326(5951):433-6.
[3]潘敏慧,余泉友,夏玉玲,代方银,刘彦群,鲁成,张泽,向仲怀.中国野桑蚕线粒体基因组特征[J].中国科学C辑:生命科学,2008,38(08):751-759.
[4]鲁成,赵爱春,向仲怀,万春玲.中国野桑蚕遗传多样性的AFLP分析[J].动物学研究,2002,(02):166-172.
[5]鲁成,余红仕,向仲怀.基于RAPD分析的中国野桑蚕和家蚕遗传多样性和系统发育关系研究[J].昆虫学报,2002,(02):198-203.
[6]鲁成,程道军,向仲怀,伴野丰,藤井博.中国野桑蚕和日本野桑蚕的RAPD研究[J].蚕业科学,2002,(01):17-21.
[7]Zhang X,Nie M,Zhao Q,Wu Y,Wang G,Xia Q.Genome-wide patterns of genetic variation among silkworms[J].Mol Genet Genomics,2015,290(04):1575-87.
[8]杜周和,刘俊凤,刘斌彬,董占鹏,余泉友,鲁成,陈义安.基因amy基因的中国野桑蚕遗传多样性及其与家蚕的系统发育关系[J].昆虫学报,2009,52(12):1 338-1 348.
[9]Cheng T,Fu B,Wu Y,Long R,Liu C,Xia Q.Transcriptome sequencing and positive selected genes analysis of Bombyx mandarina[J].PLoS One,2015,10(03):e0122837.
[10]Wolstenholme DR.Animal mitochondrial DNA:structure and evolution[J].Int Rev Cytol,1992,141:173-216.
[11]Edgar RC.MUSCLE:a multiple sequence alignment method with reduced time and space complexity[J].BMC Bioinformatics,2004,(05):113.
[12]Tamura K,Stecher G,Peterson D,Filipski A,Kumar S.MEGA6:Molecular Evolutionary Genetics Analysis version 6.0[J].Mol Biol Evol,2013,30(12):2 725-9.