黄花柳3个无性系叶色及叶绿素荧光特性比较
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摘要
[目的]了解黄花柳资源的变异特性。[方法]以黄花柳3个无性系为材料,通过柯尼卡CR-400全自动色差计对其叶色参数进行测定,通过乙醇提取方法对其叶绿素含量进行测定,使用Pocket PEA快速荧光仪对其叶绿素荧光特性进行分析比较。[结果]叶绿素含量与L、a均呈显著负相关;金枝黄花柳的叶色偏黄、叶绿素含量较低,但其潜在光化学活性最强,对强光胁迫具有较强的自我保护能力;普通黄花柳叶色偏绿、叶绿素含量较高,其潜在光化学活性也较强,但其对光能的利用率最低;红枝黄花柳的叶色较金枝黄花柳偏绿,较普通黄花柳偏黄,其叶绿素含量与光能利用率均介于二者之间。[结论]叶绿素含量越高叶片颜色越偏绿色且越明亮;金枝黄柳较其他2个黄花柳无性系叶片颜色更鲜艳,且其光化学活性最强,对光能的利用率最大。
[Objective]To know about the variation characteristics of the resources of Salix caprea.[Method]We used three clones of S. caprea as experimental materials,determined its leaf color parameters by Konica CR-400 and its chlorophyll content by ethanol extraction method. The fluorescence characteristics of the chlorophyll were analyzed and compared using the Pocket PEA fast fluorometer.[Result]The chlorophyll content was negatively correlated with L-value and a-value. S. caprea ‘GoldenStem' had yellow color leaves and lower chlorophyll content, but its photochemical activity was the strongest, so it had strong self-protection ability against strong light stress.For common S.aprea,it's leaf color was greenish, the chlorophyll content was higher, and its potential photochemical activity was stronger.The color of S. caprea ‘RedenStem' was greenish compared with the S.aprea‘GoldenStem', the chlorophyll content and utilization of light energy were between the two clones.[Conclusion]The higher the chlorophyll content, the more green and bright the leaves are. The color of S.aprea ‘GoldenStem' is more vivid than the other two clones, and S.aprea‘GoldenStem' had strongest photochemical activity and maximum utilization of light energy.
[Objective]To know about the variation characteristics of the resources of Salix caprea.[Method]We used three clones of S. caprea as experimental materials,determined its leaf color parameters by Konica CR-400 and its chlorophyll content by ethanol extraction method. The fluorescence characteristics of the chlorophyll were analyzed and compared using the Pocket PEA fast fluorometer.[Result]The chlorophyll content was negatively correlated with L-value and a-value. S. caprea ‘GoldenStem' had yellow color leaves and lower chlorophyll content, but its photochemical activity was the strongest, so it had strong self-protection ability against strong light stress.For common S.aprea,it's leaf color was greenish, the chlorophyll content was higher, and its potential photochemical activity was stronger.The color of S. caprea ‘RedenStem' was greenish compared with the S.aprea‘GoldenStem', the chlorophyll content and utilization of light energy were between the two clones.[Conclusion]The higher the chlorophyll content, the more green and bright the leaves are. The color of S.aprea ‘GoldenStem' is more vivid than the other two clones, and S.aprea‘GoldenStem' had strongest photochemical activity and maximum utilization of light energy.
引文
[1] 施士争,王红玲,周洁.观赏柳树资源多样性及应用展望[J].林业科技开发,2015,29(2):1-6.
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[10] 张辉.夜景照明对5种蔷薇科植物叶绿素荧光参数影响的差异研究[J].江西农业学报,2017,29(6):35-39.
[11] STRASSER R J,SRIVASTAVA A,TSIMILLI-MICHAEL M.The fluorescence transient as a tool to characterize and screen photosynthetic samples[M]//YUNUS M,PATHRE U,MOHANTY P,et al.Probing photosynthesis:Mechanism,regulation and adaptation.London:Taylor and Francis Press,2000:445-483.
[12] STRASSER R J,TSIMILLI-MICHAEL M,SRIVASTAVA A.Analysis of the chlorophyll a fluorescence transient[M]//Chlorophyll a fluorescence.Netherlands:Springer,2004:321-362.
[13] STRASSER R J,GOVINDJEE.The fo and the O-J-I-P fluorescence rise in higher plants and algae[M]//ARGYROUDI-AKOYUNOGLOU J H.Regulation of chloroplast biogenesis.New York:Plenum Press,1992:423-426.
[14] STRASSER R J,GOVINDJEE.On the O-J-I-P fluorescence transient in leaves and D1 mutants of Chlamydomonas reinhardtii[J].Photosynthesis research,1992,34(1):29-32.
[15] 郭欢欢,刘勇,姚飞,等.不同种源黄连木秋季色素含量与叶色参数的关系[J].西北植物学报,2017,37(10):2003-2009.
[16] 胡敬志,田旗,鲁心安.枫香叶片色素含量变化及其与叶色变化的关系[J].西北农林科技大学学报(自然科学版),2007,35(10):219-223.
[17] 赵会杰,邹琦,于振文.叶绿素荧光分析技术及其在植物光合机理研究中的应用[J].河南农业大学学报,2000,34(3):248-251.
[18] 王宝松,潘明建,郭群,等.柳树杂种及无性系耐水性遗传变异的研究[J].江苏林业科技,2000,27(5):1-6.
[2] 涂忠虞,潘明建.柳树造纸及矿柱用材优良无性系选育[J].江苏林业科技,1997,24(1):1-6,21.
[3] 涂中虞,沈熙环.中国林木遗传育种进展[M].北京:科学技术文献出版社,1993.
[4] 安守芹,乌云塔娜,周凤娴,等.沙柳优良无性系选择[J].内蒙古农业大学学报(自然科学版),2001,22(1):49-54.
[5] 王春荣,梁海永,刘晶芳,等.张承地区野生柳树资源调查报告[J].河北林业科技,2017(3):35-38.
[6] 刘晶芳,王志彬,梁海永.绚丽海棠实生后代叶色及形态研究[J].河北林业科技,2018(1):1-6.
[7] 张云海,何念鹏,张光明,等.氮沉降强度和频率对羊草叶绿素含量的影响[J].生态学报,2013,33(21):6786-6794.
[8] BJ?RKMAN O,DEMMIG B.Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins[J].Planta,1987,170(4):489-504.
[9] NGUYEN BA TRIEU,陈宇,林小琴,等.不同种源越南杉木幼龄期叶绿素荧光特征比较[J].四川农业大学学报,2016,34(1):34-38.
[10] 张辉.夜景照明对5种蔷薇科植物叶绿素荧光参数影响的差异研究[J].江西农业学报,2017,29(6):35-39.
[11] STRASSER R J,SRIVASTAVA A,TSIMILLI-MICHAEL M.The fluorescence transient as a tool to characterize and screen photosynthetic samples[M]//YUNUS M,PATHRE U,MOHANTY P,et al.Probing photosynthesis:Mechanism,regulation and adaptation.London:Taylor and Francis Press,2000:445-483.
[12] STRASSER R J,TSIMILLI-MICHAEL M,SRIVASTAVA A.Analysis of the chlorophyll a fluorescence transient[M]//Chlorophyll a fluorescence.Netherlands:Springer,2004:321-362.
[13] STRASSER R J,GOVINDJEE.The fo and the O-J-I-P fluorescence rise in higher plants and algae[M]//ARGYROUDI-AKOYUNOGLOU J H.Regulation of chloroplast biogenesis.New York:Plenum Press,1992:423-426.
[14] STRASSER R J,GOVINDJEE.On the O-J-I-P fluorescence transient in leaves and D1 mutants of Chlamydomonas reinhardtii[J].Photosynthesis research,1992,34(1):29-32.
[15] 郭欢欢,刘勇,姚飞,等.不同种源黄连木秋季色素含量与叶色参数的关系[J].西北植物学报,2017,37(10):2003-2009.
[16] 胡敬志,田旗,鲁心安.枫香叶片色素含量变化及其与叶色变化的关系[J].西北农林科技大学学报(自然科学版),2007,35(10):219-223.
[17] 赵会杰,邹琦,于振文.叶绿素荧光分析技术及其在植物光合机理研究中的应用[J].河南农业大学学报,2000,34(3):248-251.
[18] 王宝松,潘明建,郭群,等.柳树杂种及无性系耐水性遗传变异的研究[J].江苏林业科技,2000,27(5):1-6.