醋酸杆菌辅助酶解法提取动物软骨中硫酸软骨素的研究
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摘要
本实验以猪、羊等动物软骨为原料,采用醋酸杆菌和两种蛋白酶消化,以硫酸软骨素得率和纯度为指标,通过多次实验,获得的最佳提取工艺条件为醋酸杆菌浓度6g/100mL,发酵温度35℃;碱性蛋白酶浓度1.5%,酶解时间4 h;木瓜蛋白酶浓度1.5%,酶解时间4 h。在此条件下硫酸软骨素提取率为7.65%,纯度为92.77%。
The articles introduced a method of extraction chondroitin sulfate from bones of animals. With the extraction rate of chondroitin sulfate as the evaluation index, the extraction condition for chondroitin sulfate was optimized by single factor test. Under the conditions of 1.5% alkali protease concentration, enzymolysis for4 h,1.5% of papainprotease, enzymolysis for 4 h. Under the best extraction conditions, the white powder of CS can be obtained with the average yield rate of 7.65%,the purity of 92.77%.
The articles introduced a method of extraction chondroitin sulfate from bones of animals. With the extraction rate of chondroitin sulfate as the evaluation index, the extraction condition for chondroitin sulfate was optimized by single factor test. Under the conditions of 1.5% alkali protease concentration, enzymolysis for4 h,1.5% of papainprotease, enzymolysis for 4 h. Under the best extraction conditions, the white powder of CS can be obtained with the average yield rate of 7.65%,the purity of 92.77%.
引文
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[6]吴梧桐,陈琼华,徐碧如.银耳孢子多糖的分离和分析[J].真菌学报,2002,1(2):119-124.
[7]Volpi N.Quality of different chondroitin sulfate preparations in relation to their the rapeutic activity[J].J Pharm Pharmacol,2009,61:1271-1280.
[2]杨勇,陈旭,咎中学,等.盐酸氨基葡萄糖联合硫酸软骨素治疗腰椎小关节骨关节炎的临床观察[J].华西医学,2012,27(12):1809-1813.
[3]王亚,陈彦,夏树开,等.硫酸软骨素制备工艺的优化[J].中国生化药物杂志,2006,27(1):12-14.
[4]陈守平.鲟鱼硫酸软骨素制备工艺的研究[D].福州:福建农林大学,2007.
[5]高华,刘坤,于兹东,等.间苯三酚分光光度法测定硫酸软骨素的研究[J].中国生化药物杂志,2010,21(5):247-248.
[6]吴梧桐,陈琼华,徐碧如.银耳孢子多糖的分离和分析[J].真菌学报,2002,1(2):119-124.
[7]Volpi N.Quality of different chondroitin sulfate preparations in relation to their the rapeutic activity[J].J Pharm Pharmacol,2009,61:1271-1280.