大果阿魏石油醚提取物成分分析及其对胃黏膜保护作用的评价
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摘要
目的:明晰新疆大果阿魏石油醚提取物中的物质组成及其对乙酸损伤型大鼠胃黏膜的保护作用,为胃黏膜保护及抗氧化类功能食品的开发提供借鉴。方法:采用乙酸所致大鼠胃黏膜损伤模型,将60只SD大鼠分为正常组、模型组、阳性组(雷尼替丁0.03 g/kg),大果阿魏石油醚提取物分为高、中、低剂量组(1.0、0.2、0.04 g/kg m_b),均连续灌胃两周,并采用气相色谱-质谱联用技术分析大果阿魏石油醚提取物中的化学成分。修复效果以胃黏膜损伤面积变化为评价指标,并计算损伤抑制率。同时,测定胃组织中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、前列腺素E_2(prostaglandin E_2,PGE_2)及一氧化氮(NO)含量。结果:大果阿魏石油醚提取物中共分离鉴定出25种物质,其中萜类化合物含量最多,相对含量为49.47%,其次是含硫化合物,相对含量为24.01%。各剂量大果阿魏石油醚提取物组均能明显改善大鼠胃黏膜损伤程度,与其他实验组相比,低、中、高剂量组损伤面积平均减小了7.68～13.10 mm~2;与模型组相比,高剂量提取物组效果最佳,损伤抑制率达到31.84%,SOD活力增加了37.27%,MDA含量降低了32.51%,PGE_2和NO含量分别增加了22.41%和28.13%。结论:大果阿魏石油醚提取物对大鼠乙酸性胃黏膜损伤具有明显的保护作用,作用机制可能与其提高胃黏膜抗氧化能力有关。
Purpose: The present study aimed to characterize the chemical profile of petroleum ether extract from Ferula lehmannii Boiss and evaluate its gastroprotective activity, so as to provide an experimental basisfor the development of functional foods with antioxidant activity and gastric mucosal protection. Methods: The gastroprotective activity of Ferula lehmannii Boiss was investigated using an animal model of gastric ulcer induced by acetic acid. Totally 60 rats were divided into normal, model, ranitidine(positive control, at 0.03 g/kg), and high, medium and low-dose petroleum ether extract groups(1.0, 0.2 and 0.04 g/kg m_b). The components of the extract were analyzed by gas-phase chromatography-mass spectrometry(GC-MS). The administration lasted for 2 weeks. At the end of the experimental period, all rats were sacrificed to collect stomach samples for evaluation of antioxidant indexes including superoxide dismutase(SOD) activity and malondialdehyde(MDA) level, and protective factors of gastric mucosa such as prostaglandin E_2(PGE_2) and nitric oxide(NO). The change in gastric mucosal injury area was considered to evaluate the protective effect of the extract against gastric mucosal injury, and the percentage of injury inhibition was calculated. Results: Totally 25 compounds were identified from the extract, including terpenoids(49.47%) and sulphur-containing compounds(24.01%). The petroleum ether extract significantly attenuated gastric mucosal damage and reduced lesion area by 7.68 to 13.10 mm~2 on average compared with other experimental groups.Compared with the model group, the extract at the high dose gave the maximum percentage of injury inhibition of 31.84%,increased SOD activity by 37.27%, decreased MDA level by 32.51%, and augmented the contents of PEG_2 and NO by22.41% and 28.13%. Conclusion: The petroleum ether extract from Ferula lehmannii Boiss can exert protective effect against chronic gastric mucosal injury, and the mechanism may be associated with the enhancement of antioxidant capacity.
Purpose: The present study aimed to characterize the chemical profile of petroleum ether extract from Ferula lehmannii Boiss and evaluate its gastroprotective activity, so as to provide an experimental basisfor the development of functional foods with antioxidant activity and gastric mucosal protection. Methods: The gastroprotective activity of Ferula lehmannii Boiss was investigated using an animal model of gastric ulcer induced by acetic acid. Totally 60 rats were divided into normal, model, ranitidine(positive control, at 0.03 g/kg), and high, medium and low-dose petroleum ether extract groups(1.0, 0.2 and 0.04 g/kg m_b). The components of the extract were analyzed by gas-phase chromatography-mass spectrometry(GC-MS). The administration lasted for 2 weeks. At the end of the experimental period, all rats were sacrificed to collect stomach samples for evaluation of antioxidant indexes including superoxide dismutase(SOD) activity and malondialdehyde(MDA) level, and protective factors of gastric mucosa such as prostaglandin E_2(PGE_2) and nitric oxide(NO). The change in gastric mucosal injury area was considered to evaluate the protective effect of the extract against gastric mucosal injury, and the percentage of injury inhibition was calculated. Results: Totally 25 compounds were identified from the extract, including terpenoids(49.47%) and sulphur-containing compounds(24.01%). The petroleum ether extract significantly attenuated gastric mucosal damage and reduced lesion area by 7.68 to 13.10 mm~2 on average compared with other experimental groups.Compared with the model group, the extract at the high dose gave the maximum percentage of injury inhibition of 31.84%,increased SOD activity by 37.27%, decreased MDA level by 32.51%, and augmented the contents of PEG_2 and NO by22.41% and 28.13%. Conclusion: The petroleum ether extract from Ferula lehmannii Boiss can exert protective effect against chronic gastric mucosal injury, and the mechanism may be associated with the enhancement of antioxidant capacity.
引文
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[11]王飒.新疆多伞阿魏体外抗胃癌活性部位指纹图谱研究[D].乌鲁木齐:新疆医科大学,2015:23-43.
[12]熊元君,刘发,叶尔波,等.新疆三种阿魏对胃肠道作用的比较[J].新疆医学院学报,1993(4):300-302.
[13]潘年松,张学愈,邹俊,等.水蒸汽蒸馏和石油醚浸提两法提取莪术粗粉挥发油收率比较[J].中成药,2007,29(10):1519-1521.DOI:10.3969/j.issn.1001-1528.2007.10.039.
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[15]袁松.不同分子量海带岩藻聚糖硫酸酯对大鼠抗胃溃疡作用的研究[D].青岛:中国海洋大学,2014:17-20.
[16]詹萍,田洪磊,李卫国,等.羊肉特征风味指纹图谱的构建[J].现代食品科技,2013,29(10):2522-2527.DOI:10.13982/j.mfst.1673-9078.2013.10.043.
[17]IRANSHAHI M,YAZDI M C,HASSANZADEHKHAYYAT M,et al.Sulfur containing compounds in the volatile oil of Ferula latisecta Rech.f.&Aell.leaves[J].Journal of Essential Oil Bearing Plants,2009,12(1):64-68.DOI:10.1080/0972060X.2009.10643693.
[18]李新蕊.主成分分析、因子分析、聚类分析的比较与应用[J].齐鲁师范学院学报,2007,22(6):23-26.
[19]孙桂芳.啤酒抗氧化力和风味稳定性的研究[D].广州:华南理工大学,2011:20-45.
[20]马士巧.超声辅助提取东北黑蜂蜂胶活性成分及其指纹图谱研究[D].镇江:江苏大学,2015:33-52.
[21] 陈景明,许嵘,黄晓冬,等.惠安产赤楠叶挥发油的化学成分与体外抗氧化活性评价[J].宁德师范学院学报(自然科学版),2016,28(2):147-154.DOI:10.3969/j.issn.2095-2481.2016.02.008.
[22]陈建烟.花叶艳山姜叶片精油提纯、结构鉴定及生物活性研究[D].福州:福建农林大学,2014:40-46.
[23]IRANSHAHI A S M.Volatile constituents of the genus(Apiaceae):a review[J].Journal of Essential Oil Bearing Plants,2011,14(5):504-531.DOI:10.1080/0972060X.2011.10643969.
[24]KAVOOSI G,ROWSHAN V.Chemical composition,antioxidant and antimicrobial activities of essential oil obtained from Ferula assafoetida oleo-gum-resin:effect of collection time[J].Food Chemistry,2013,138(4):2180-2187.DOI:10.1016/j.foodchem.2012.11.131.
[25]邓超澄,霍丽妮,李培源,等.广西阴香叶挥发油化学成分及其抗氧化性研究[J].中国实验方剂学杂志,2010,16(17):105-109.DOI:10.13422/j.cnki.syfjx.2010.17.039.
[26]王慧英,王金亭.玫瑰精油的研究与应用现状[J].粮食与油脂,2015,28(10):5-9.DOI:10.3969/j.issn.1008-9578.2015.10.002.
[27] 张大帅,王燕,陈光英,等.铁椤叶挥发油成分及生物活性研究[J].食品工业科技,2013,34(17):115-118.DOI:10.13386/j.issn1002-0306.2013.17.018.
[28]关鑫磊.内源性小分子二甲基硫醚对氧化应激损伤的作用及机制研究[D].武汉:华中科技大学,2015:1-29.
[29]LI W,WANG X,ZHANG H,et al.Anti-ulcerogenic effect of cavidine against ethanol-induced acute gastric ulcer in mice and possible underlying mechanism[J].International Immunopharmacology,2016,38:450-459.DOI:10.1016/j.intimp.2016.06.016.
[30]MCCORD J M,KEELE B B,FRIDOVICH I.An enzyme-based theory of obligate anaerobiosis:the physiological function of superoxide dismutase[J].Proceedings of the National Academy of Sciences of the United States of America,1971,68(5):1024-1027.
[31]NING J W,LIN G B,JI F,et al.Preventive effects of geranylgeranylacetone on rat ethanol-induced gastritis[J].World Journal of Gastroenterology,2012,18(18):2262-2269.DOI:10.3748/wjg.v18.i18.2262.
[32]张琪.绿茶中茶多酚的提取分离及其对慢性胃炎大鼠胃粘膜的保护作用[D].西安:陕西师范大学,2015:41-49.
[33]马凤余,鄢顺琴,凤良元.胃舒灵Ⅱ对大鼠胃黏膜保护因子的影响[J].中国实验方剂学杂志,2009,15(1):48-50.DOI:10.13422/j.cnki.syfjx.2009.01.019.
[2]BORATO D G,SCOPARO C T,MARIA-FERRRIRA D,et al.Healing mechanisms of the hydroalcoholic extract and ethyl acetate fraction of green tea(Camellia sinensis,(L.)Kuntze)on chronic gastric ulcers[J].Naunyn-Schmiedeberg’s Archives of Pharmacology,2015,389(3):259-268.DOI:10.1007/s00210-015-1200-8
[3]王国中,陈志伟,潘洪明,等.丹参抗大鼠乙醇性胃粘膜损伤的作用及机制[J].中国中西医结合消化杂志,2002,10(6):340-341.DOI:10.3969/j.issn.1671-038X.2002.06.007.
[4]阮研硕,赵江燕,李艳梅,等.岩藻多糖对急性酒精性胃黏膜损伤的保护作用[J].中国食品学报,2015,15(1):19-24.DOI:10.16429/j.1009-7848.2015.01.004.
[5]李肖宇,李国玉,王航宇,等.大果阿魏化学成分的研究[J].中国现代中药,2010,12(8):17-20.DOI:10.13313/j.issn.1673-4890.2010.08.007.
[6]DASTAN D,SALEHI P,GOHARI G A,et al.Disesquiterpene and sesquiterpene coumarins from Ferula pseudalliacea,and determination of their absolute configurations[J].Phytochemistry,2012,78(2):170-178.DOI:10.1016/j.phytochem.2012.02.016.
[7]ABEDI D,JALALI M,ASGHARIG,et al.Composition and antimicrobial activity of oleogumresin of Ferula gumosa Bioss.essential oil using Alamar Blue~(TM)[J].Research in Pharmaceutical Sciences,2008,3(1):41-45.
[8]POONAM M,SHRADHA B.Ferula asafoetida:traditional uses and pharmacological activity[J].Pharmacognosy Reviews,2012,6(12):141-146.DOI:10.4103/0973-7847.99948.
[9]AMIRHOSSEIN S,MOHAMMAD H K,MEHRDAD I.Qualitative analysis of the hydro-distilled essential oil of Ferula latisecta Rech.f.and Aell.roots from Iran[J].Journal of Essential Oil Bearing Plants,2010,13(3):340-346.DOI:10.1080/0972060X.2010.10643832.
[10]SAHEBKAR A,IRANSHAHI M.Biological activities of essential oils from the genus Ferula(Apiaceae)[J].Asian Biomedicine,2011,4(6):835-847.DOI:10.2478/abm-2010-0110.
[11]王飒.新疆多伞阿魏体外抗胃癌活性部位指纹图谱研究[D].乌鲁木齐:新疆医科大学,2015:23-43.
[12]熊元君,刘发,叶尔波,等.新疆三种阿魏对胃肠道作用的比较[J].新疆医学院学报,1993(4):300-302.
[13]潘年松,张学愈,邹俊,等.水蒸汽蒸馏和石油醚浸提两法提取莪术粗粉挥发油收率比较[J].中成药,2007,29(10):1519-1521.DOI:10.3969/j.issn.1001-1528.2007.10.039.
[14]OKABE S,PFEIFFER C J.Chronicity of acetic acid ulcer in the rat stomach[J].American Journal of Digestive Diseases,1972,17(7):619-629.
[15]袁松.不同分子量海带岩藻聚糖硫酸酯对大鼠抗胃溃疡作用的研究[D].青岛:中国海洋大学,2014:17-20.
[16]詹萍,田洪磊,李卫国,等.羊肉特征风味指纹图谱的构建[J].现代食品科技,2013,29(10):2522-2527.DOI:10.13982/j.mfst.1673-9078.2013.10.043.
[17]IRANSHAHI M,YAZDI M C,HASSANZADEHKHAYYAT M,et al.Sulfur containing compounds in the volatile oil of Ferula latisecta Rech.f.&Aell.leaves[J].Journal of Essential Oil Bearing Plants,2009,12(1):64-68.DOI:10.1080/0972060X.2009.10643693.
[18]李新蕊.主成分分析、因子分析、聚类分析的比较与应用[J].齐鲁师范学院学报,2007,22(6):23-26.
[19]孙桂芳.啤酒抗氧化力和风味稳定性的研究[D].广州:华南理工大学,2011:20-45.
[20]马士巧.超声辅助提取东北黑蜂蜂胶活性成分及其指纹图谱研究[D].镇江:江苏大学,2015:33-52.
[21] 陈景明,许嵘,黄晓冬,等.惠安产赤楠叶挥发油的化学成分与体外抗氧化活性评价[J].宁德师范学院学报(自然科学版),2016,28(2):147-154.DOI:10.3969/j.issn.2095-2481.2016.02.008.
[22]陈建烟.花叶艳山姜叶片精油提纯、结构鉴定及生物活性研究[D].福州:福建农林大学,2014:40-46.
[23]IRANSHAHI A S M.Volatile constituents of the genus(Apiaceae):a review[J].Journal of Essential Oil Bearing Plants,2011,14(5):504-531.DOI:10.1080/0972060X.2011.10643969.
[24]KAVOOSI G,ROWSHAN V.Chemical composition,antioxidant and antimicrobial activities of essential oil obtained from Ferula assafoetida oleo-gum-resin:effect of collection time[J].Food Chemistry,2013,138(4):2180-2187.DOI:10.1016/j.foodchem.2012.11.131.
[25]邓超澄,霍丽妮,李培源,等.广西阴香叶挥发油化学成分及其抗氧化性研究[J].中国实验方剂学杂志,2010,16(17):105-109.DOI:10.13422/j.cnki.syfjx.2010.17.039.
[26]王慧英,王金亭.玫瑰精油的研究与应用现状[J].粮食与油脂,2015,28(10):5-9.DOI:10.3969/j.issn.1008-9578.2015.10.002.
[27] 张大帅,王燕,陈光英,等.铁椤叶挥发油成分及生物活性研究[J].食品工业科技,2013,34(17):115-118.DOI:10.13386/j.issn1002-0306.2013.17.018.
[28]关鑫磊.内源性小分子二甲基硫醚对氧化应激损伤的作用及机制研究[D].武汉:华中科技大学,2015:1-29.
[29]LI W,WANG X,ZHANG H,et al.Anti-ulcerogenic effect of cavidine against ethanol-induced acute gastric ulcer in mice and possible underlying mechanism[J].International Immunopharmacology,2016,38:450-459.DOI:10.1016/j.intimp.2016.06.016.
[30]MCCORD J M,KEELE B B,FRIDOVICH I.An enzyme-based theory of obligate anaerobiosis:the physiological function of superoxide dismutase[J].Proceedings of the National Academy of Sciences of the United States of America,1971,68(5):1024-1027.
[31]NING J W,LIN G B,JI F,et al.Preventive effects of geranylgeranylacetone on rat ethanol-induced gastritis[J].World Journal of Gastroenterology,2012,18(18):2262-2269.DOI:10.3748/wjg.v18.i18.2262.
[32]张琪.绿茶中茶多酚的提取分离及其对慢性胃炎大鼠胃粘膜的保护作用[D].西安:陕西师范大学,2015:41-49.
[33]马凤余,鄢顺琴,凤良元.胃舒灵Ⅱ对大鼠胃黏膜保护因子的影响[J].中国实验方剂学杂志,2009,15(1):48-50.DOI:10.13422/j.cnki.syfjx.2009.01.019.