香茅草黄酮提取物对L-NNA诱导的高血压大鼠血压及炎性因子的影响
|
摘要
目的探讨香茅草黄酮提取物对左旋硝基精氨酸(L-NNA)诱导的高血压大鼠血压的影响及其作用机制。方法采用N,-硝基-L-精氨酸(L-NNA)15 mg/(kg·d)腹腔注射给药4周复制高血压动物模型,选择平均动脉压高于140 mmHg的大鼠50只进行实验,将其随机分为5组,即模型组、香茅草黄酮提取物低剂量、中剂量、高剂量组、卡托普利阳性药物组,另外选取正常10只大鼠作为空白组。模型组和空白对照组给予同等体积生理盐水;阳性药物对照组大鼠给予1%卡托普利2 mL/d;香茅草黄酮提取物低(0.85%)、中(1.65%)、高(3.35%)浓度剂量组给予香茅草黄酮提取物2 mL/d,各组连续灌胃给药4周,每周测定大鼠尾动脉血压。4周后,采用开腹腔后,用真空采血管于腹主动脉取血,用ELISA法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的水平。结果给药后,香茅草黄酮提取物低、中、高剂量组及阳性药物对照组与模型组比较,呈现明显的降压作用(P <0.05)。与空白组相比,模型组大鼠血清中TNF-α,IL-6含量显著升高(P <0.05)。4周后,香茅草黄酮提取物中、高剂量组以及卡托普利阳性药物组大鼠血清中TNF-α、IL-6含量低于模型组,差异有统计学意义(P <0.05)。结论香茅草黄酮提取物对L-NNA诱导的高血压有一定的降压作用,并能有效降低高血压的炎症水平,其降压机制可能与炎性因子有关,具体的抗炎机制还有待于进一步探究。
Objective To investigate the effect of flavonoids extract from cymbopogon on blood pressure induced by L-NNA in hypertensive rats, and to study the mechanism of its effect on blood pressure. Methods Rats with N'-nitro-L-arginine(L-NNA) 15 mg/(kg·d) were administered intraperitoneally for four weeks to replicate the animal model of hypertension. 50 rats with an average arterial pressure higher than 140 mmHg were selected for the experiment and randomly divided into five groups, namely model group, cymbopogon low dose, medium dose and high dose group,captopril positive drug group, and another ten normal rats were selected as the blank group. The model group and the blank control group were given the same volume physiological saline. The rats in the positive control group were given1% captopril 2 mL/d; low(0.85%), medium(1.65%) and high(3.35%) concentration dose group were given 2 mL/d of cymbopogon extract, and each group was given a continuous gavage for four weeks, and the arterial blood pressure was measured twice a week and the serum levels of TNF-α and IL-6 were measured by ELISA. Results Compared with the model group, cymbopogon low dose, medium dose and high dose group, captopril positive drug group showed obvious hypotensive effect(P < 0.05). Compared with the blank group, the serum TNF-α and IL-6 in the model group were significantly increased(P < 0.05). After four weeks, the serum TNF-α and IL-6 in the medium and high dose group and captopril group were lower than those in the model group(P <0.05). Conclusion Cymbopogon extracts of L-NNA induced hypertension has certain function, and can reduce the inflammation level of hypertension effectively. Its function mechanism may be related to inflammatory factors, and the specific anti-inflammatory mechanism remains to be further explored.
Objective To investigate the effect of flavonoids extract from cymbopogon on blood pressure induced by L-NNA in hypertensive rats, and to study the mechanism of its effect on blood pressure. Methods Rats with N'-nitro-L-arginine(L-NNA) 15 mg/(kg·d) were administered intraperitoneally for four weeks to replicate the animal model of hypertension. 50 rats with an average arterial pressure higher than 140 mmHg were selected for the experiment and randomly divided into five groups, namely model group, cymbopogon low dose, medium dose and high dose group,captopril positive drug group, and another ten normal rats were selected as the blank group. The model group and the blank control group were given the same volume physiological saline. The rats in the positive control group were given1% captopril 2 mL/d; low(0.85%), medium(1.65%) and high(3.35%) concentration dose group were given 2 mL/d of cymbopogon extract, and each group was given a continuous gavage for four weeks, and the arterial blood pressure was measured twice a week and the serum levels of TNF-α and IL-6 were measured by ELISA. Results Compared with the model group, cymbopogon low dose, medium dose and high dose group, captopril positive drug group showed obvious hypotensive effect(P < 0.05). Compared with the blank group, the serum TNF-α and IL-6 in the model group were significantly increased(P < 0.05). After four weeks, the serum TNF-α and IL-6 in the medium and high dose group and captopril group were lower than those in the model group(P <0.05). Conclusion Cymbopogon extracts of L-NNA induced hypertension has certain function, and can reduce the inflammation level of hypertension effectively. Its function mechanism may be related to inflammatory factors, and the specific anti-inflammatory mechanism remains to be further explored.
引文
[1]国家卫生计生委合理用药专家委员会,中国医师协会高血压专业委员会.高血压合理用药指南(第2版)[J].中国医学前沿杂志(电子版),2017,9(7):28-126.
[2]CELIK A,KO?F,KADI H,et al.Infl ammation is related to unbalanced cardiac autonomic functions in hypertension:an observational study[J].Anadolu Kardiyol Derg,2012,12(3):233-240.
[3]BLAKE G L,RIFAI N,BURING J E,et al.Blood pressure,C-reactive protein,and risk of future cardiovascular events[J].Circulation,2003(108):2993-2999.
[4]TIWARI M,DWIVEDI U N,KAKKAR P.Suppression of oxidative stress and pro-inflammatory mediators by Cymbopogon citrates D.Stapf extract in lipopolysaccharide stimulated murine alveolar macrophages.Food Chem Toxicol,2010,48(10):2913-2919.
[5]FIGUEIRINHA A,CRUZ M V,LOPES M C,et al.Antiinfl ammatory activity of Cymbopogon citrates leaf infusion in lipopolysaccharide-Stimulated dendritic cells:comtribution of the polyphenols.J Med food,2010,13(3):681-690.
[6]刁水华.香茅提取物微胶囊的制备及棉纶针织物的驱蚊整理[D].苏州:苏州大学,2015.
[7]何以红,胡展豪,付裕,等.芦荟提取物对左旋硝基精氨酸诱导的高血压大鼠影响的研究[J].中华全科医学,2017,15(3):407-410,474.
[8]刘杨.降压敷贴联合耳穴埋豆治疗原发性高血压[J].长春中医药大学学报,2015,31(5):998-1003.
[9]时晓迟,柳威,王娟,等.天麻钩藤饮对自发性高血压大鼠心脏Bax及Caspase-3蛋白表达的影响[J].天津中医药,2016,33(6):354-357.
[10]周春红,高向阳,胡薇.原发性高血压患者肾素-血管紧张素-醛固酮系统活性的影响因素分析[J].中国社区医师,2018,34(2):113,115.
[11]CELIK A,KO?F,KADI H,et al.Inflammation is related to unbalanced cardiac autonomic functions in hypertension:an observational study[J].Anadolu Kardiyol Derg,2012,12(3):233-240.
[12]BOMFIM G F,ECHEM C,MARTINS C B,et al.Tolllike receptor 4 inhibition reduces vascular inflammation in spontaneously hypertensive rats[J].Life Sciences,2015(122):1-7.
[13]BLAKE GL,RIFAI N,BURING JE,et al.Blood pressure,C-reactive protein,and risk of future cardiovascular events[J].Circulation,2003(108):2993-2999.
[14]曾勇,谭超,叶舒婷,等.复方钩藤降压片对自发性高血压大鼠血压及炎症水平的影响[J].中华中医药学刊,2014,32(12):2983-2985.
[15]谢蒂立,程标,金静.厄贝沙坦治疗高血压合并冠心病患者疗效及对血管内皮功能和炎性因子的影响[J].解放军医药杂志,2018,30(2):78-81.
[16]周兆鹏,许祖建,戴小华.原发性高血压与炎症因子相关性及中医药干预研究[J].中医药临床杂志,2015,27(3):307-309.
[17]刘倍吟,魏程科,李应东.当归挥发油对高血压模型大鼠血压及血管炎症反应的影响[J].中国中医药信息杂志,2016,23(11):71-74.
[18]赵娜,马维红,苏赢,等.牛蒡根水提物对高血压大鼠血管内皮损伤的保护作用[J].天津医药,2015,43(1):42-45.
[19]OKURA T,JOTOKU M,IRITA J,et al.Association between cystatin C and inflammation in patients with essential hypertension[J].ClinExpNephrol,2010,14(6):584-588.
[20]张艳利,吴建龙,乔建勇,等.肿瘤坏死因子-α及内皮素-1在高血压脑出血血肿周围脑组织中表达及意义[J].中国老年学杂志,2018,38(4):938-940.
[2]CELIK A,KO?F,KADI H,et al.Infl ammation is related to unbalanced cardiac autonomic functions in hypertension:an observational study[J].Anadolu Kardiyol Derg,2012,12(3):233-240.
[3]BLAKE G L,RIFAI N,BURING J E,et al.Blood pressure,C-reactive protein,and risk of future cardiovascular events[J].Circulation,2003(108):2993-2999.
[4]TIWARI M,DWIVEDI U N,KAKKAR P.Suppression of oxidative stress and pro-inflammatory mediators by Cymbopogon citrates D.Stapf extract in lipopolysaccharide stimulated murine alveolar macrophages.Food Chem Toxicol,2010,48(10):2913-2919.
[5]FIGUEIRINHA A,CRUZ M V,LOPES M C,et al.Antiinfl ammatory activity of Cymbopogon citrates leaf infusion in lipopolysaccharide-Stimulated dendritic cells:comtribution of the polyphenols.J Med food,2010,13(3):681-690.
[6]刁水华.香茅提取物微胶囊的制备及棉纶针织物的驱蚊整理[D].苏州:苏州大学,2015.
[7]何以红,胡展豪,付裕,等.芦荟提取物对左旋硝基精氨酸诱导的高血压大鼠影响的研究[J].中华全科医学,2017,15(3):407-410,474.
[8]刘杨.降压敷贴联合耳穴埋豆治疗原发性高血压[J].长春中医药大学学报,2015,31(5):998-1003.
[9]时晓迟,柳威,王娟,等.天麻钩藤饮对自发性高血压大鼠心脏Bax及Caspase-3蛋白表达的影响[J].天津中医药,2016,33(6):354-357.
[10]周春红,高向阳,胡薇.原发性高血压患者肾素-血管紧张素-醛固酮系统活性的影响因素分析[J].中国社区医师,2018,34(2):113,115.
[11]CELIK A,KO?F,KADI H,et al.Inflammation is related to unbalanced cardiac autonomic functions in hypertension:an observational study[J].Anadolu Kardiyol Derg,2012,12(3):233-240.
[12]BOMFIM G F,ECHEM C,MARTINS C B,et al.Tolllike receptor 4 inhibition reduces vascular inflammation in spontaneously hypertensive rats[J].Life Sciences,2015(122):1-7.
[13]BLAKE GL,RIFAI N,BURING JE,et al.Blood pressure,C-reactive protein,and risk of future cardiovascular events[J].Circulation,2003(108):2993-2999.
[14]曾勇,谭超,叶舒婷,等.复方钩藤降压片对自发性高血压大鼠血压及炎症水平的影响[J].中华中医药学刊,2014,32(12):2983-2985.
[15]谢蒂立,程标,金静.厄贝沙坦治疗高血压合并冠心病患者疗效及对血管内皮功能和炎性因子的影响[J].解放军医药杂志,2018,30(2):78-81.
[16]周兆鹏,许祖建,戴小华.原发性高血压与炎症因子相关性及中医药干预研究[J].中医药临床杂志,2015,27(3):307-309.
[17]刘倍吟,魏程科,李应东.当归挥发油对高血压模型大鼠血压及血管炎症反应的影响[J].中国中医药信息杂志,2016,23(11):71-74.
[18]赵娜,马维红,苏赢,等.牛蒡根水提物对高血压大鼠血管内皮损伤的保护作用[J].天津医药,2015,43(1):42-45.
[19]OKURA T,JOTOKU M,IRITA J,et al.Association between cystatin C and inflammation in patients with essential hypertension[J].ClinExpNephrol,2010,14(6):584-588.
[20]张艳利,吴建龙,乔建勇,等.肿瘤坏死因子-α及内皮素-1在高血压脑出血血肿周围脑组织中表达及意义[J].中国老年学杂志,2018,38(4):938-940.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |