献血前丙氨酸氨基转移酶初筛阈值的确定
|
摘要
目的研究和确定流动献血丙氨酸氨基转移酶(Alanine aminotransferase,ALT)初筛阈值,减少血液报废。方法先应用干化学方法对血液样本进行初筛,对ALT水平>40 U/L且<50 U/L的样本使用全自动生化仪进行复检。对初筛值和复检值建立线性回归方程,计算出ALT初筛的阈值,并依此为基准计算出2017年新旧阈值对应的复检不合格率。结果通过对100份血液样本的分析,计算出线性回归方程y=1.046 4x-0.433,相关系数R值为0.905 3,ALT检测新阈值为47 U/L。以50 U/L为检测阈值,2017年上半年复检不合格率为0.020%;以47 U/L为检测阈值,2017年下半年复检不合格率为0.012%,有效降低了复检不合格率。结论针对不同仪器和试剂确定初筛ALT阈值,可以降低复检不合格率,减少血液报废。
Objective To study and determine the preliminary screening threshold of Alanine amtransferinoase(ALT) and reduce blood scrap rate. Methods The blood samples were firstly screened by dry chemical method, and the ALT level of the samples greater than 40 U/L and less than50 U/L were re-examined by automatic biochemical analyzer. The linear regression equation was established with the initial screening value and the retest value, and the threshold value of ALT preliminary screening was calculated. Based on the new threshold value, the failure rate of reexamination in 2017 was calculated. Results Through the analysis of 100 blood samples, the linear regression equation y=1.046 4 x-0.433 was calculated, the correlation coefficient R value was 0.905 3, and the new threshold value for ALT detection was 47 U/L. Taking 50 U/L as the test threshold, the failure rate of re-inspection in the first half of 2017 is 0.020%. Taking 47 U/L as the test threshold, the failure rate of re-inspection in the second half of 2017 is 0.012%, effectively reducing the failure rate of re-inspection. Conclusion The determination of ALT threshold value of preliminary screening using different instruments and reagents can reduce the retest failure rate and blood scrap.
Objective To study and determine the preliminary screening threshold of Alanine amtransferinoase(ALT) and reduce blood scrap rate. Methods The blood samples were firstly screened by dry chemical method, and the ALT level of the samples greater than 40 U/L and less than50 U/L were re-examined by automatic biochemical analyzer. The linear regression equation was established with the initial screening value and the retest value, and the threshold value of ALT preliminary screening was calculated. Based on the new threshold value, the failure rate of reexamination in 2017 was calculated. Results Through the analysis of 100 blood samples, the linear regression equation y=1.046 4 x-0.433 was calculated, the correlation coefficient R value was 0.905 3, and the new threshold value for ALT detection was 47 U/L. Taking 50 U/L as the test threshold, the failure rate of re-inspection in the first half of 2017 is 0.020%. Taking 47 U/L as the test threshold, the failure rate of re-inspection in the second half of 2017 is 0.012%, effectively reducing the failure rate of re-inspection. Conclusion The determination of ALT threshold value of preliminary screening using different instruments and reagents can reduce the retest failure rate and blood scrap.
引文
[1]王瑞,黄力勤,张婧,等.实施核酸检测后重新评估献血者ALT的检测功效[J].中国输血杂志,2017,30(9):1034-1038.
[2]李锋,王银锋,师志云,等.运用标准差指数和变异系数指数评价实验室内不同血细胞分析仪检测结果的一致性[J].中华临床实验室管理电子杂志,2016,4(1):37-42.
[3]卢志芳,杨先凯,李凤侠.室间不同生化检测系统结果可比性探讨[J].实验与检验医学,2014,31(1):34-36.
[4]郭敏璇,黄凌峰.献血前干式生化法丙氨酸氨基转移酶初筛合适限值的确定[J].现代医院,2015,14(15):91-92.
[5]中华人民共和国国家标准.献血者健康检告要求[S].GB18467.2001:9-12.
[6] Granér M, Varpula M, Kahri J, et al. Association of carotid intima-media thickness with angiographic severity and extent of coronary artery disease[J]. Am J Cardiol, 2006,97(5):624-629.
[7] MulèG, Cottone S, MongiovìR, et al. Influence of the metabolic syndrome on aortic stiffness in never treated hypertensive patients[J]. Nutr Metab Cardiovasc Dis, 2006,16(1):54-59.
[8]王凯,刘玉振,方建华,等.干化学试纸条法快速初筛献血者ALT漏检原因分析[J].中国误诊学杂志,2004,4(1):52-53.
[9]高均翠.干化学法在无偿献血者ALT初筛中的应用分析[J].中国输血杂志,2011,24(11):977-978.
[10]赵建鹏,杨秀平,刘晓丽,等.干、湿化学方法检测献血者ALT对比分析[J].现代医学检验杂志,2013,28(1):48-49.
[11]周宝琴,杨思忠,李秋华,等.青岛地区ALT初筛前后无偿献血者检测结果分析[J].中国输血杂志,2011,24(10):872-873.
[12]邢培清,刘玉振,李伍升,等.影响干化学法初筛献血者ALT因素探讨[J].中国输血杂志,204,17(6):447.
[2]李锋,王银锋,师志云,等.运用标准差指数和变异系数指数评价实验室内不同血细胞分析仪检测结果的一致性[J].中华临床实验室管理电子杂志,2016,4(1):37-42.
[3]卢志芳,杨先凯,李凤侠.室间不同生化检测系统结果可比性探讨[J].实验与检验医学,2014,31(1):34-36.
[4]郭敏璇,黄凌峰.献血前干式生化法丙氨酸氨基转移酶初筛合适限值的确定[J].现代医院,2015,14(15):91-92.
[5]中华人民共和国国家标准.献血者健康检告要求[S].GB18467.2001:9-12.
[6] Granér M, Varpula M, Kahri J, et al. Association of carotid intima-media thickness with angiographic severity and extent of coronary artery disease[J]. Am J Cardiol, 2006,97(5):624-629.
[7] MulèG, Cottone S, MongiovìR, et al. Influence of the metabolic syndrome on aortic stiffness in never treated hypertensive patients[J]. Nutr Metab Cardiovasc Dis, 2006,16(1):54-59.
[8]王凯,刘玉振,方建华,等.干化学试纸条法快速初筛献血者ALT漏检原因分析[J].中国误诊学杂志,2004,4(1):52-53.
[9]高均翠.干化学法在无偿献血者ALT初筛中的应用分析[J].中国输血杂志,2011,24(11):977-978.
[10]赵建鹏,杨秀平,刘晓丽,等.干、湿化学方法检测献血者ALT对比分析[J].现代医学检验杂志,2013,28(1):48-49.
[11]周宝琴,杨思忠,李秋华,等.青岛地区ALT初筛前后无偿献血者检测结果分析[J].中国输血杂志,2011,24(10):872-873.
[12]邢培清,刘玉振,李伍升,等.影响干化学法初筛献血者ALT因素探讨[J].中国输血杂志,204,17(6):447.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |