树莓酒主发酵期活性成分含量变化及降血脂作用研究
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摘要
探讨树莓酒在主发酵期间总酚、总黄酮及树莓酮含量的变化,研究树莓酒的降血脂作用。结果显示,主发酵期总酚含量从2.230 g/L提高到2.330 g/L;总黄酮含量从0.164 g/L提高到0.298 g/L。高效液相色谱(high performance liquid chromatography,HPLC)测定树莓酮含量,其含量从2.300μg/mL提高到9.456μg/mL。各活性成分浸出为树莓酒成品的降血脂功效奠定基础。动物实验结果显示:高剂量树莓酒(0.76 g/kg)降血脂效果最佳,实验第4周以后体重与模型组相比差异显著(p<0.05),甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(lowdensity lipoprotein cholesterol,LDL-C)含量与模型组相比有显著性(p<0.05),表明树莓酒在一定剂量范围内有较好的辅助降血脂功效,值得进一步研究和开发。
The contents of total phenol,total flavonoids,and raspberry ketone during the main fermentation of raspberry wine were discussed,and the effect of raspberry wine on lowering blood lipid was studied.The results showed that the total phenol content during the main fermentation period increased from 2.230 g/L to 2.330 g/L,and the total flavonoid content increased from 0.164 g/L to 0.298 g/L.The content of raspberry ketone was increased from 2.300 μg/mL to 9.456 μg/mL determined by HPLC.Lowering lipid of raspberry wine was contributed by the increasing of active ingredients content.The results indicated that the body weight and lower the levels of TG,TC,and LDL-C of hyperlipidemic rats were reduced with high dose raspberry wine(0.76 g/kg)compared with the mode l group significantly(p<0.05)after four weeks of experiment.It showed that the raspberry wine had a certain hypolipidemic capacity.It was deserved to be further research and development.
The contents of total phenol,total flavonoids,and raspberry ketone during the main fermentation of raspberry wine were discussed,and the effect of raspberry wine on lowering blood lipid was studied.The results showed that the total phenol content during the main fermentation period increased from 2.230 g/L to 2.330 g/L,and the total flavonoid content increased from 0.164 g/L to 0.298 g/L.The content of raspberry ketone was increased from 2.300 μg/mL to 9.456 μg/mL determined by HPLC.Lowering lipid of raspberry wine was contributed by the increasing of active ingredients content.The results indicated that the body weight and lower the levels of TG,TC,and LDL-C of hyperlipidemic rats were reduced with high dose raspberry wine(0.76 g/kg)compared with the mode l group significantly(p<0.05)after four weeks of experiment.It showed that the raspberry wine had a certain hypolipidemic capacity.It was deserved to be further research and development.
引文
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[20]旷慧,冯建文,范倩,等.红树莓多酚的组分分析及体外抗脂质过氧化活性[J].食品科学,2018,568(3):83-89
[21]Saem J H,Sohyeon K,Jun H S,et al.Black raspberry extract increased circulating endothelial progenitor cells and improved arterial stiffness in patients with metabolic syndrome:A randomized controlled trial[J].J.Med.Food,2016,19(4):346-352
[22]石永芳.覆盆子的营养成分和药理作用的研究进展[J].山东化工,2017,46(6):71-72
[23]Fotschki B,Jurgonski A,Jukiewicz J,et al.Dietary supplementation with raspberry seed oil modulates liver functions,inflammatory state,and lipid metabolism in rats[J].J Nutr,2015,145(8):1793-1799
[24]Baselga E L,Pascual S A,Ribas L A,et al.Long-term supplementation with a low dose of proanthocyanidins normalized liver miR-33a and miR-122 levels in high-fat diet-induced obese rats[J].Nutrition Research,2015,35(4):337-345
[2]司旭,陈芹芹,毕金峰,等.树莓主要功能性成分研究进展[J].食品工业科技,2015,36(4):376-381
[3]孙金旭.覆盆子干果黄酮提取研究[J].食品研究与开发,2012,33(9):14-17
[4]Aspeea F J,Theodulozb C,Avila F,et al.The Chilean wild raspberry(Rubus geoides Sm.)increases intracellular GSH content and protects against H2O2and methylglyoxal-induced damage in AGScells[J].Food Chemistry,2016,194(1):908-919
[5]周兰华.桔梗多酚的提取、纯化及抗氧化活性研究[D].长春:长春工业大学,2015
[6]张海悦,王黎兵,郭新力.狭叶荨麻总黄酮的测定[J].食品科技,2007,12(17):181-184
[7]李斌,王小杰,杨磊,等.HPLC-MS/MS法测定树莓中树莓酮含量的研究[J].生物技术进展,2013,18(6):439-442
[8]国家食品药品监督管理局.GB/T107-2012辅助降血脂功能评价方法[S].北京:中国医药科技出版社,2011
[9]Park K S.Raspberry ketone,a naturally occurring phenolic compound,inhibits adipogenic and lipogenic gene expression in 3T3-L1 adipocytes[J].Pharm Biol,2015,53(6):870-875
[10]于润美,张海悦,翟硕,等.树莓活性成分及其果酒加工工艺研究进展[J].酿酒科技,2017,272(2):109-112
[11]于润美,姜燕,张海悦,等.复合澄清剂对红树莓果酒的澄清效果研究[J].食品研究与开发,2017,38(22):127-132
[12]中华人民共和国国家标准.GB/T 15038-2006葡萄酒,果酒通用分析方法[S].北京:中国标准化委员会出版社,2005
[13]Li C,Zhang M,Zheng H,et al.Effects of tanshinone II sodium sulfonate plus cinepazide maleate on the hemorrheologic indexes and blood lipids in patients with acute cerebral infarction[J].Neural Regeneration Research,2007,2(4):11-17
[14]Yin Z G,Wang Q S,Yu K,et al.Sex differences in associations between blood lipids and cerebral small vessel disease[J].Nutrition,Metabolism and Cardiovascular Diseases,2017(5):122-129
[15]Vasim K,Sumit S,Uma B,et al.Raspberry ketone protects against isoproterenol-induced myocardial infarction in rats[J].Life Sciences,2018,21(1):194-200
[16]Swi諭tkiewicz I D,Piotr S P.Effects of plastic mulches and high tunnel raspberry production systems on oil physicochemical quality indicators[J].International Agrophysics,2018,32(1):12-17
[17]刘淑敏.不同茶类浸提液及茶多酚的生物活性和机理研究[D].广州:华南理工大学,2016
[18]代燕丽.桑叶多酚的质量标准及其抗氧化和脂质代谢调节作用研究[D].广州:广东药科大学,2016
[19]华雨薇,李春阳,王帆,等.蓝莓叶多酚对高脂小鼠血脂及肝脏组织的影响[J].食品科学,2016,528(11):222-225
[20]旷慧,冯建文,范倩,等.红树莓多酚的组分分析及体外抗脂质过氧化活性[J].食品科学,2018,568(3):83-89
[21]Saem J H,Sohyeon K,Jun H S,et al.Black raspberry extract increased circulating endothelial progenitor cells and improved arterial stiffness in patients with metabolic syndrome:A randomized controlled trial[J].J.Med.Food,2016,19(4):346-352
[22]石永芳.覆盆子的营养成分和药理作用的研究进展[J].山东化工,2017,46(6):71-72
[23]Fotschki B,Jurgonski A,Jukiewicz J,et al.Dietary supplementation with raspberry seed oil modulates liver functions,inflammatory state,and lipid metabolism in rats[J].J Nutr,2015,145(8):1793-1799
[24]Baselga E L,Pascual S A,Ribas L A,et al.Long-term supplementation with a low dose of proanthocyanidins normalized liver miR-33a and miR-122 levels in high-fat diet-induced obese rats[J].Nutrition Research,2015,35(4):337-345
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