党参多糖的表征分析及抗氧化性质的研究

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英文篇名：Characterization analysis of codonopsis pilosula polysaccharide and its effects of anti-active opxygen 作者：夏洁 ; 徐长远 ; 俞启扬 ; 陈雪娇 ; 张西锋 英文作者：XIA Jie;XU Chang-yuan;YU Qi-yang;CHEN Xue-jiao;ZHANG Xi-feng;School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University; 关键词：党参 ; 多糖 ; 抗氧化 英文关键词：codonopsisilosula;;polysaccharide;;anti-avtive oxygen 中文刊名：WHSP 英文刊名：Journal of Wuhan Polytechnic University 机构：武汉轻工大学生物与制药工程学院; 出版日期：2019-06-15 出版单位：武汉轻工大学学报 年：2019 期：v.38;No.139 基金：国家自然科学基金(B020704) 语种：中文; 页：WHSP201903002 页数：4 CN：03 ISSN：42-1856/T 分类号：5-7+35

摘要

采用水提醇沉法得到党参多糖(CPP),并采用SEM和FTIR对党参多糖进行表征分析、GPC-RI-MALS检测党参多糖的分子量。研究党参多糖对ABTS自由基、羟基自由基以及金属离子的清除活性。结果表明:党参多糖表观呈团聚状态,多糖中两种多糖组分的分子量为Mn为1.482×10~6、Mw为4.629×10~7和Mn为3.753×10~3、Mw为5.692×10~3。党参多糖对亚铁离子、羟基自由基和ABTS自由基均具有非常好的清除活性并在CCP浓度为10 mg/mL时清除率达到最大,分别为92.8%、82.3%和73%,且具有浓度依赖性。
        In this experiment,codonopsis pilosula polysaccharide(CPP) was obtained by water extraction and alcohol precipitation,the characterization analysis was analyzed by SEM and FTIR,and the molecular weight of CPP was detected by GPC-RI-MALS. The scavenging activity of CPP on ABTS radical,hydroxyl radical and metal ion was studied. The results showed that the CPP appeared to be agglomerated,the molecular weight of two CPP,Mn is 1.482×10~6 and Mw is 4.629×10~7,the other`s Mn is 3.753×10~3 and its Mw is 5.692×10~3. CPP has strong scavenging activities to ABTS,metal ions and hydrogen radical scavenging. With the increase of CPP concentration,the ability of moving activity is strengthened. At the concentration of 10 mg/mL,the largest scavenging of are separately 92.8%,82.3% and 73%.

引文

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