北京地区汉族人群MTHFR C677T基因多态性分析
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摘要
目的探索北京地区汉族MTHFR基因多态性分布特征,荟萃(meta)分析北京地区与北方其他地区MTHFR基因多态性分布差异。方法用PCR-金磁微粒层析法检测MTHFR C677T基因,回顾性分析北京协和医院2014年9月至2018年5月3 945例体检健康者MTHFR基因多态性分布特征。查阅中外文献数据库,并对北京地区与北方其他地区汉族的MTHFR基因多态性分布比较。结果北京地区体检健康人群男性MTHFR C677T基因CC、CT和TT基因型频率分别为23.3%,50.5%和26.2%,C、T等位基因频率分别为48.6%和51.4%。女性人群MTHFR C677T基因CC、CT和TT基因型频率分别为22.7%,49.4%和27.9%,C、T等位基因频率分别为47.4%和52.6%,男、女性基因型频率与等位基因频率差异无统计学意义(P>0.05)。北京地区体检人群MTHFR C677T基因CC、CT和TT基因型频率与等位基因频率均与黑龙江、吉林、河北、山东和河南等省差异有统计学意义(P<0.01)。结论北京地区体检人群MTHFR C677T基因多态性在男、女性人群中的分布无差异;北京地区MTHFR基因分布有自身特点。
Objective To explore the distribution characteristics of MTHFR gene polymorphism of Chinese Han population in Beijing,and analyze the differences of distribution of MTHFR gene polymorphism between Beijing area and the other parts of northern China.Methods MTHFR C677T gene was detected by PCR-gold magnetic particles chromatography.The distribution characteristics of MTHFR gene polymorphism of 3 945 healthy subjects from September 2014 to May 2018 detected in Peking Union Medical College Hospital were analyzed retrospectively.The distribution of MTHFR gene polymorphism in Chinese Han population in Beijing area was compared with the Han population of the other area from northern China by reviewing domestic and foreign literature databases.Results The frequencies of CC,CT and TT genotype of MTHFR C677T gene in the male population undergoing physical examination in Beijing were23.3%,50.5% and 26.2%,respectively,and the frequencies of C and T allele frequencies were 48.6% and 51.4%,respectively.The frequencies of CC,CT and TT genotype of MTHFR C677T gene in the female population were 22.7%,49.4% and 27.9%,respectively,and the C and T allele frequencies were 47.4% and 52.6%,respectively.There was no difference in genotype frequency and allele freaaency between male and femal(P>0.05).The frequencies of CC,CT and TT genotypes and allele frequencies of MTHFR C677T gene in the population of Beijing area were significantly different from those of Heilongjiang,Jilin,Hebei,Shandong and Henan provinces(P<0.01).Conclusion There was no significant difference of polymorphism distribution of MTHFR C677T gene between male and female populations in Beijing.The distinct distribution characteristics of MTHFR gene in Beijing area should be presented.
Objective To explore the distribution characteristics of MTHFR gene polymorphism of Chinese Han population in Beijing,and analyze the differences of distribution of MTHFR gene polymorphism between Beijing area and the other parts of northern China.Methods MTHFR C677T gene was detected by PCR-gold magnetic particles chromatography.The distribution characteristics of MTHFR gene polymorphism of 3 945 healthy subjects from September 2014 to May 2018 detected in Peking Union Medical College Hospital were analyzed retrospectively.The distribution of MTHFR gene polymorphism in Chinese Han population in Beijing area was compared with the Han population of the other area from northern China by reviewing domestic and foreign literature databases.Results The frequencies of CC,CT and TT genotype of MTHFR C677T gene in the male population undergoing physical examination in Beijing were23.3%,50.5% and 26.2%,respectively,and the frequencies of C and T allele frequencies were 48.6% and 51.4%,respectively.The frequencies of CC,CT and TT genotype of MTHFR C677T gene in the female population were 22.7%,49.4% and 27.9%,respectively,and the C and T allele frequencies were 47.4% and 52.6%,respectively.There was no difference in genotype frequency and allele freaaency between male and femal(P>0.05).The frequencies of CC,CT and TT genotypes and allele frequencies of MTHFR C677T gene in the population of Beijing area were significantly different from those of Heilongjiang,Jilin,Hebei,Shandong and Henan provinces(P<0.01).Conclusion There was no significant difference of polymorphism distribution of MTHFR C677T gene between male and female populations in Beijing.The distinct distribution characteristics of MTHFR gene in Beijing area should be presented.
引文
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[17]路广平,鲁衍强,薛琰,等.河北省廊坊市广阳区汉族女性MTHFR、MTRR基因多态性分布特征分析[J].中国妇幼保健,2016,31(16):3316-3319.
[18]宋妙丽,朱耿超,魏佳玲,等.苏州地区育龄妇女MTHFRC677T基因多态性分布[J].贵州医科大学学报,2017,42(7):808-811.
[19]贺宪民,张群,杨琦,等.亚甲基四氢叶酸还原酶和甲硫氨酸合成酶还原酶基因多态性研究[J].中国计划生育学杂志,2010,18(1):13-18.
[20]张艳丽,李华锋,伏广照,等.临沂地区汉族女性MTHFR和MTRR基因多态性分布研究[J].山东医学高等专科学校学报,2013,35(6):401-404.
[21]从玉英,鲁衍强,芮欣忆,等.淄博市汉族女性亚甲基四氢叶酸还原酶和甲硫氨酸合成酶还原酶基因多态性分布研究[J].现代妇产科进展,2012,21(10):779-781.
[22]陈小利,鲁衍强,马少杰,等.山东省济源市汉族女性MTHFR与MTRR基因多态性分布研究[J].中国妇幼卫生杂志,2014,5(4):17-20.
[23]严倩,鲁衍强,李瑛,等.烟台市汉族女性MTHFR和MTRR基因多态性的分布特征[J].山东大学学报(医学版),2014,52(1):79-84.
[24]矫富燕,鲁衍强,李瑛,等.烟台市牟平区汉族女性亚甲基四氢叶酸还原酶和甲硫氨酸合成酶还原酶基因多态性分布研究[J].分子诊断与治疗杂志,2014,6(2):105-109.
[25]何燕霞,巩姣梅,沈勇,等.河南省育龄女性叶酸利用能力现状研究[J].实用检验医师杂志,2012,4(1):6-10.
[26]崔会玲,鲁衍强,马少杰,等.郑州市汉族女性MTHFR和MTRR基因多态性分布[J].中南大学学报(医学版),2015,40(7):710-714.
[27]吕雪,徐金义,李涛,等.河南汉族人群MTHFR基因多态性及同型半胱氨酸与冠心病关系研究[J].中华实用诊断与治疗杂志,2015,29(6):603-604,607.
[28]陈云霞,程红兵,武延隽,等.长治人群MTHFR C677T基因多态性分布与血浆Hcy水平[J].山西医科大学学报,2013,44(2):130-133.
[29]孙文萍,万琪,苏明权.西安地区汉族亚甲基四氢叶酸还原酶的两种基因多态性[J].医学争鸣,2003,24(7):634-636.
[30]方莉,母春兰,张玲琳,等.四川东北部地区孕妇MTHFRC677T基因多态性分布研究[J].基因组学与应用生物学,2018,37(4):1820-1824.
[31]王淑媛,鲁衍强,马少杰,等.湘潭市汉族女性MTHFR和MTRR基因多态性分布及其与血浆Hcy水平的关系[J].天津医药,2014,42(12):1205-1209.
[2]杨文方,王加丽,张彤,等.陕西西安地区汉族孕龄女性MTHFR基因多态性的分布研究[J].中国生育健康杂志,2017,28(3):243-247.
[3]马路密,鲁衍强,李瑛,等.尚志市汉族女性MTHFR与MTRR基因多态性调查[J].实用预防医学,2015,22(3):289-291.
[4]李晓宏,鲁衍强,李瑛,等.齐齐哈尔市汉族女性叶酸代谢通路关键酶MTHFR基因多态性的分布情况[J].中国妇幼保健,2016,31(11):2341-2343.
[5]方芳,方舟,沈庆明,荆洪英.MTHFR C677T基因多态性在佳木斯地区健康人及复发性流产患者中的分布频率[J].黑龙江医药科学,2017,40(6):54-55.
[6]李焱,鲁衍强,薛琰,等.吉林省汉族女性MTHFR、MTRR基因型和等位基因频率分布与其他地区的比较[J].中国妇幼保健,2015,30(32):5612-5614.
[7]于延辉,鲁衍强,李瑛,等.延边朝鲜族自治州汉族女性和朝鲜族女性MTHFR与MTRR基因多态性分布研究[J].实用医学杂志,2014,30(5):7-9.
[8]咸峰元.延边地区缺血性脑卒中患者MTHFR C677T基因多态性分析[D].吉林:延边大学,2013:1-46.
[9]李桂华,鲁衍强,李瑛,等.辽源市汉族女性MTHFR和MTRR的遗传多态性分析[J].中国妇幼保健,2016,31(10):2130-2132.
[10]周漫,刘欢,李艳.武汉市汉族女性MTHFR C677T基因多态性分布研究[J].中国热带医学,2017,17(11):1063-1066.
[11]田红雨,鲁衍强,付敏,等.长春市汉族妇女MTHFR与MTRR基因多态性分布研究[J].中国妇幼保健,2017,32(9):1962-1965.
[12]Yang B,Liu Y,Li Y,et al.Geographical distribution of MTHFRC677T,A1298C and MTRR A66G gene polymorphisms in China:findings from 15357 adults of Han nationality[J].PLoS One,2013,8(3):e57917.
[13]谢晓媛,冷俊宏,鲁衍强,等.女性MTHFR、MTRR基因型和等位基因频率分布与其他地区女性的比较[J].中国优生与遗传杂志,2016,24(3):12-14.
[14]高峡,鲁衍强,马少杰,等.张家口地区汉族女性MTHFR和MTRR基因多态性分布[J].中国优生与遗传杂志,2014,22(11):35-37.
[15]李俊华,鲁衍强,马少杰,等.廊坊市汉族女性MTHFR与MTRR基因多态性分析[J].基础医学与临床,2014,34(11):1564-1565.
[16]靳慧云,鲁衍强,付敏,等.保定市涞水县汉族女性MTHFR、MTRR基因型与等位基因频率分布和其他地区汉族女性的比较[J].中国妇幼保健,2016,31(21):4500-4503.
[17]路广平,鲁衍强,薛琰,等.河北省廊坊市广阳区汉族女性MTHFR、MTRR基因多态性分布特征分析[J].中国妇幼保健,2016,31(16):3316-3319.
[18]宋妙丽,朱耿超,魏佳玲,等.苏州地区育龄妇女MTHFRC677T基因多态性分布[J].贵州医科大学学报,2017,42(7):808-811.
[19]贺宪民,张群,杨琦,等.亚甲基四氢叶酸还原酶和甲硫氨酸合成酶还原酶基因多态性研究[J].中国计划生育学杂志,2010,18(1):13-18.
[20]张艳丽,李华锋,伏广照,等.临沂地区汉族女性MTHFR和MTRR基因多态性分布研究[J].山东医学高等专科学校学报,2013,35(6):401-404.
[21]从玉英,鲁衍强,芮欣忆,等.淄博市汉族女性亚甲基四氢叶酸还原酶和甲硫氨酸合成酶还原酶基因多态性分布研究[J].现代妇产科进展,2012,21(10):779-781.
[22]陈小利,鲁衍强,马少杰,等.山东省济源市汉族女性MTHFR与MTRR基因多态性分布研究[J].中国妇幼卫生杂志,2014,5(4):17-20.
[23]严倩,鲁衍强,李瑛,等.烟台市汉族女性MTHFR和MTRR基因多态性的分布特征[J].山东大学学报(医学版),2014,52(1):79-84.
[24]矫富燕,鲁衍强,李瑛,等.烟台市牟平区汉族女性亚甲基四氢叶酸还原酶和甲硫氨酸合成酶还原酶基因多态性分布研究[J].分子诊断与治疗杂志,2014,6(2):105-109.
[25]何燕霞,巩姣梅,沈勇,等.河南省育龄女性叶酸利用能力现状研究[J].实用检验医师杂志,2012,4(1):6-10.
[26]崔会玲,鲁衍强,马少杰,等.郑州市汉族女性MTHFR和MTRR基因多态性分布[J].中南大学学报(医学版),2015,40(7):710-714.
[27]吕雪,徐金义,李涛,等.河南汉族人群MTHFR基因多态性及同型半胱氨酸与冠心病关系研究[J].中华实用诊断与治疗杂志,2015,29(6):603-604,607.
[28]陈云霞,程红兵,武延隽,等.长治人群MTHFR C677T基因多态性分布与血浆Hcy水平[J].山西医科大学学报,2013,44(2):130-133.
[29]孙文萍,万琪,苏明权.西安地区汉族亚甲基四氢叶酸还原酶的两种基因多态性[J].医学争鸣,2003,24(7):634-636.
[30]方莉,母春兰,张玲琳,等.四川东北部地区孕妇MTHFRC677T基因多态性分布研究[J].基因组学与应用生物学,2018,37(4):1820-1824.
[31]王淑媛,鲁衍强,马少杰,等.湘潭市汉族女性MTHFR和MTRR基因多态性分布及其与血浆Hcy水平的关系[J].天津医药,2014,42(12):1205-1209.