甜玉米雄性不育系T9501不育基因的初定位
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摘要
2014年,在海南田间繁种过程中获得可以稳定遗传的甜玉米雄性不育突变体T9501;2016年,以B73为父本,与T9501杂交,得到F1;2017年,套袋自交得到F2群体。本研究中,以亲本、F1及F2群体为试验材料,进行花粉育性鉴定及遗传分析。花粉育性鉴定结果显示,不育株花药内花粉极少且没有育性。遗传分析表明,不育性状由1对细胞核隐性基因控制;利用128对Indel标记对亲本与F2进行多态性检测和连锁分析,将该不育基因初步定位在甜玉米6号染色体短臂(6.01bin)上,暂命名为MS9501,后通过开发加密标记,初步确定目的基因位于标记Indel130与SNP2之间,相对遗传距离分别为0.91 cM和8.10 cM,物理距离约6.51 Mb。
T9501, a sterile mutant of sweet maize, was obtained from a field breeding in Hainan in 2014. It was subjected to mutant hybridization with B73 as the father, resulting in F1 in 2016. The F1 self–crossed and generate F2 in 2017. Using F2 group and its parental origin as testing materials, the pollen fertility identification showed that the pollen was little and sterile. Their genetic analysis revealed that the infertility traits were controlled by a pair of recessive genes. Polymorphism and linkage analysis of F2 and its parental origin using 128 pairs of insertion–deletion markers indicated that the target gene was located in the sterile gene preliminary maize chromosome 6 short arm(6.01 bin), named MS9501 in this study. Using a encryption labeling method developed, we determined the target genes was located in marking Indel130 between SNP2. The relative genetic recombination rate distance were 0.91 cM and 8.10 cM, with physical distance about 6.51 Mb.
T9501, a sterile mutant of sweet maize, was obtained from a field breeding in Hainan in 2014. It was subjected to mutant hybridization with B73 as the father, resulting in F1 in 2016. The F1 self–crossed and generate F2 in 2017. Using F2 group and its parental origin as testing materials, the pollen fertility identification showed that the pollen was little and sterile. Their genetic analysis revealed that the infertility traits were controlled by a pair of recessive genes. Polymorphism and linkage analysis of F2 and its parental origin using 128 pairs of insertion–deletion markers indicated that the target gene was located in the sterile gene preliminary maize chromosome 6 short arm(6.01 bin), named MS9501 in this study. Using a encryption labeling method developed, we determined the target genes was located in marking Indel130 between SNP2. The relative genetic recombination rate distance were 0.91 cM and 8.10 cM, with physical distance about 6.51 Mb.
引文
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[13]康丹.玉米ms2雄性不育突变体的表型分析与基因定位[D].北京:中国农业科学院,2016.
[2]吕庆雪,高嵩,孙蕾,等.玉米雄性不育基因Zmms1的同源克隆及生物信息学分析[J].玉米科学,2018,26(2):44-52.
[3]万向元,吴锁伟,谢科.一种控制玉米雄性生育力的Zm Ms7基因序列及其编码蛋白:CN201410338212.0[P].2014-07-16.
[4]TIMOFEJEVA L,SKIBBE D S,LEE S,et al.Cytological characterization and allelism testing of anther developmental mutants identified in a screen of maize male sterile lines[J].G3:Genes,Genomes,Genetics,2013,3(2):231-249.
[5]YU Z,WU S,ZHANG D,et al.Genetic analysis and gene mapping of recessive genic male sterility14(ms14)mutant in maize[J].China Biotechnology,2016,36(10):8-14.
[6]ALBERTSEN M C,FOX T,LEONARD A,et al.Cloning and use of the ms9 gene from maize:U.S.14/207,855[P].2015-07-09.
[7]CHAUBAL R,ANDERSON J R,TRIMNELL M R,et al.The transformation of anthers in the msca1 mutant of maize[J].Planta,2003,216(5):778-788.
[8]ALBERTSEN M C,FOX T,TRIMNELL M,et al.Msca1nucleotide sequences impacting plant male fertility and method of using same:U.S.915,478[P].2011-03-29.
[9]VERNOUD V,LAIGLE G,ROZIER F,et al.The HD-ZIP IV transcription factor ocl4 is necessary for trichome patterning and anther development in maize[J].The Plant Journal,2009,59(6):883-894.
[10]MOON J,SKIBBE D,TIMOFEJEVA L,et al.Regulation of cell divisions and differentiation by male sterility 32 is required for anther development in maize[J].The Plant Journal,2013,76(4):592-602.
[11]CIGAN A M,UNGER E,XU R J,et al.Phenotypic complementation of ms45 maize requires tapetal expression of MS45[J].Sexual Plant Reproduction,2001,14(3):135-142.
[12]TRIMNELL M R,FOX T W,ALBERTSEN M C.New chromosome 10 male-sterile mutant:ms47[J].Maize Genetics Cooperation Newsletter,2002,76:38.
[13]康丹.玉米ms2雄性不育突变体的表型分析与基因定位[D].北京:中国农业科学院,2016.
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