烟草种子成熟过程抗氧化酶活性变化与种子活力的关系
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摘要
研究不同成熟时期烟草种子抗氧化酶活性变化规律,分析其与种子活力之间的关系,为烟草种子成熟采收提供判断依据。以‘红花大金元’、‘MS云烟87’、‘MSK326’和‘云烟97’不同成熟时期的种子为试验材料,测定萌发率、总超氧化物歧化酶(T-SOD)、过氧化物酶(POD)含量和过氧化氢酶(CAT)活性变化。4个烟草品种种子在成熟过程中,萌发率逐渐升高,授粉后35 d达80%以上,总超氧化物歧化酶(T-SOD)和过氧化物酶(POD)的活性随着种子的成熟而下降,下降速度具体表现为先快后慢。而过氧化氢酶(CAT)活性变化趋势则表现为先上升后下降,成熟后期又有上升。结合烟草种子抗氧化酶活性的变化同表征种子活力指标之一的萌发率的相关性分析表明,总超氧化物歧化酶和过氧化物酶的活性同种子萌发率呈极显著线性负相关。随着烟草种子的成熟,萌发率逐渐升高,T-SOD和POD活性逐渐降低,T-SOD和POD活性的变化可以作为快速判断种子活力并决定种子采收时间的重要依据。
This research was established to investigate the antioxidant enzyme activity variation of tobacco seed in different mature periods, and study the relationship between antioxidant enzyme activity and seed vigor, so as to provide reference for judging the mature of tobacco seeds. Tobacco seeds of Honghuadajinyuan, MS Yunyan 87,MS K326 and Yunyan 97 in different mature periods were collected as test materials to investigate the germination rate, activity change of T-SOD, POD and CAT. The germination rate of 4 tobacco varieties increased gradually in the process of maturation, which reached more than 80% in 35 d after pollination. The activity of T-SOD and POD decreased with the mature of seeds, and decrease speed was fast first and then slow. While the activity of CAT increased first and then decreased, and increased again during maturation. Changes of antioxidant enzyme activities in tobacco seeds and the correlation analysis of germination rate of seed indicated that the activity of T-SOD and POD had extremely significant linear negative correlation with seed germination rate. As the maturity of tobacco seed, seed germination rate increased, while the activity of T-SOD and POD decreased. Therefore, the activity of T-SOD and POD could be used to determine time for seed harvest.
This research was established to investigate the antioxidant enzyme activity variation of tobacco seed in different mature periods, and study the relationship between antioxidant enzyme activity and seed vigor, so as to provide reference for judging the mature of tobacco seeds. Tobacco seeds of Honghuadajinyuan, MS Yunyan 87,MS K326 and Yunyan 97 in different mature periods were collected as test materials to investigate the germination rate, activity change of T-SOD, POD and CAT. The germination rate of 4 tobacco varieties increased gradually in the process of maturation, which reached more than 80% in 35 d after pollination. The activity of T-SOD and POD decreased with the mature of seeds, and decrease speed was fast first and then slow. While the activity of CAT increased first and then decreased, and increased again during maturation. Changes of antioxidant enzyme activities in tobacco seeds and the correlation analysis of germination rate of seed indicated that the activity of T-SOD and POD had extremely significant linear negative correlation with seed germination rate. As the maturity of tobacco seed, seed germination rate increased, while the activity of T-SOD and POD decreased. Therefore, the activity of T-SOD and POD could be used to determine time for seed harvest.
引文
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Shang S.B.,Guo J.J.,Wang Y.H.,Chen R.L.,Liu J.C.,Zhao Z.G.,and Zeng J.,2014,Effects of fruit maturity on seed germination and seedling growth performance of Vatica mangachapoi Zhongzi(Seed),33(7):27-29(尚帅斌,郭俊杰,汪奕衡,陈仁利,刘建春,赵志刚,曾杰,2014,果实成熟度对青皮种子萌发及幼苗生长的影响,种子,33(7):27-29)
Sun Y.L.,2012,The study on seed development and indicators of physiological and biochemical of cucumber,pumpkin and cucurbit,Thesis for M.S.,Shandong Agricultural University,Supervisor:Wang X.F.,and Sun X.L.,pp.16-36(孙玉良,2012,黄瓜,南瓜和瓠瓜种子发育过程中生理生化指标的研究,硕士学位论文,山东农业大学,导师:王秀峰,孙小镭,pp.16-36)
Wang P.,Zhou D.W.,and Valentine I.,2006,Seed maturity and harvest time effects seed quantity and quality of Hordeumbrevisubulatum,Seed Sci.Technol.,34(1):125-132
Xia M.X.,Wang W.,Yuan R.,Deng F.N.,and Shen F.F.,2015,Superoxide dismutase and its research in plant stress-tolerance,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(11):2633-2646(夏民旋,王维,袁瑞,邓粉妮,沈法富,2015,超氧化物歧化酶与植物抗逆性,分子植物育种,13(11):2633-2646)
Zhan Y.F.,Tian Y.S.,and Ma J.,2011,Study on relation between seed maturity and germination rate of Hot Pepper,Jiangxi Nongye Xuebao(Acta Agriculturae Jiangxi),23(9):33-35(詹永发,田应书,马俊,2011,辣椒种子成熟度与发芽率之间关系的研究,江西农业学报,23(9):33-35)
Zhang H.X.,Si L.T.,and Tang H.X.,2014,Changes of enzymatic activities of amylase,lipase and peroxidase in cucumber seed during germination,Shenyang Nongye Daxue Xuebao(Journal of Shenyang Agricultural University),45(1):83-86(张蕙心,司龙亭,唐慧殉,2014,黄瓜种子萌发中淀粉酶、脂肪酶和过氧化物酶活性变化,沈阳农业大学学报,45(1):83-86)
Zhang J.,Huo S.P.,Feng Y.C.,Zhang X.D.,Yan Q.J.,Zhang F.K.,Yu Z.F.,Xiang Z.F.,Chen K.F.,and Zou Y.C.,2012,Effects of seed maturity degree on seedling growth in maize,Zuowu Zazhi(Crops),5:96-99(张健,霍仕平,冯云超,张兴端,晏庆九,张芳魁,余志江,向振凡,陈克富,邹永翠,2012,种子成熟度对玉米幼苗生长的影响,作物杂志,5:96-99)
Zhang Y.H.,Wen C.Y.,2010,On cell membrane lipid peroxidation and changes of oxide resistance enzymes activities during the ripeness of Maize seeds,Xiangfan Xueyuan Xuebao(Journal of Xiangfan University),21(5):43-46(张艳红,文才艺,2010,玉米种子成熟过程中细胞膜脂过氧化及体内抗氧化酶活性变化的研究,襄樊学院学报,21(5):43-46)
Zhang Z.Y.,Zhu J.H.,Cheng Y.,Zhao X.L.,Li J.,Ru Z.,and Liu M.J.,2014,Seed vigor and its correlation with physiological characteristics of different maturity wheat seeds,Henan Nongye Kexue(Journal of Henan Agricultural Sciences),43(12):6-9(张自阳,朱俊华,程媛,赵新亮,李金,茹振,刘明久,2014,不同成熟度小麦种子活力及其与生理性状的相关性研究,河南农业科学,43(12):6-9)
Zheng Y.Y.,Yu X.M.,Suo W.L.,Niu Y.Z.,Deng S.B.,and Ma W.G.,2015,Study on the change and judgment of tobacco seed maturity,Zhiwu Fenlei Yu Ziyuan Xuebao(Plant Diversity and Resources),37(4):472-478(郑昀晔,禹晓梅,索文龙,牛永志,邓胜斌,马文广,2015,烟草种子成熟变化及判定方法研究,植物分类与资源学报,37(4):472-478)
Elias S.G.,and Copeland L.O.,2001,Physiological and harvest maturity of canola in relation to seed quality,Agron.J.,93(5):1054-1058
He X.J.,and Gao S.,2008,Changes of antioxidant enzyme and phenylalanine ammonia-lyas activities during Chinmonanthus Praecox seed maturation,Z.Naturforsch.C.,68(7-8):60-69
Li J.H.,Wang F.,Liao Y.L.,and Liu W.G.,2009,Advanced onseed vigor physiological-biochemical and genetic mechanisms in rice,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),7(4):772-777(李金华,王丰,廖亦龙,柳武革,2009,水稻种子活力的生理生化及遗传研究,分子植物育种,7(4):772-777)
Patiman A.,Lin C.Y.,Aygul T.,and Maryamgul M.,2010,Study on the seed substances content and germination ability during the ripening process of Allium galanthum L.,Beifang Yuanyi(Northern Horticulture),(20):66-68(帕提曼·阿布都热合曼,林辰壹,阿依古丽·铁木儿,买热艳木古丽·木合塔尔,2010,实葶葱种子成熟过程中物质变化和萌发能力研究,北方园艺,(20):66-68)
Shang S.B.,Guo J.J.,Wang Y.H.,Chen R.L.,Liu J.C.,Zhao Z.G.,and Zeng J.,2014,Effects of fruit maturity on seed germination and seedling growth performance of Vatica mangachapoi Zhongzi(Seed),33(7):27-29(尚帅斌,郭俊杰,汪奕衡,陈仁利,刘建春,赵志刚,曾杰,2014,果实成熟度对青皮种子萌发及幼苗生长的影响,种子,33(7):27-29)
Sun Y.L.,2012,The study on seed development and indicators of physiological and biochemical of cucumber,pumpkin and cucurbit,Thesis for M.S.,Shandong Agricultural University,Supervisor:Wang X.F.,and Sun X.L.,pp.16-36(孙玉良,2012,黄瓜,南瓜和瓠瓜种子发育过程中生理生化指标的研究,硕士学位论文,山东农业大学,导师:王秀峰,孙小镭,pp.16-36)
Wang P.,Zhou D.W.,and Valentine I.,2006,Seed maturity and harvest time effects seed quantity and quality of Hordeumbrevisubulatum,Seed Sci.Technol.,34(1):125-132
Xia M.X.,Wang W.,Yuan R.,Deng F.N.,and Shen F.F.,2015,Superoxide dismutase and its research in plant stress-tolerance,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(11):2633-2646(夏民旋,王维,袁瑞,邓粉妮,沈法富,2015,超氧化物歧化酶与植物抗逆性,分子植物育种,13(11):2633-2646)
Zhan Y.F.,Tian Y.S.,and Ma J.,2011,Study on relation between seed maturity and germination rate of Hot Pepper,Jiangxi Nongye Xuebao(Acta Agriculturae Jiangxi),23(9):33-35(詹永发,田应书,马俊,2011,辣椒种子成熟度与发芽率之间关系的研究,江西农业学报,23(9):33-35)
Zhang H.X.,Si L.T.,and Tang H.X.,2014,Changes of enzymatic activities of amylase,lipase and peroxidase in cucumber seed during germination,Shenyang Nongye Daxue Xuebao(Journal of Shenyang Agricultural University),45(1):83-86(张蕙心,司龙亭,唐慧殉,2014,黄瓜种子萌发中淀粉酶、脂肪酶和过氧化物酶活性变化,沈阳农业大学学报,45(1):83-86)
Zhang J.,Huo S.P.,Feng Y.C.,Zhang X.D.,Yan Q.J.,Zhang F.K.,Yu Z.F.,Xiang Z.F.,Chen K.F.,and Zou Y.C.,2012,Effects of seed maturity degree on seedling growth in maize,Zuowu Zazhi(Crops),5:96-99(张健,霍仕平,冯云超,张兴端,晏庆九,张芳魁,余志江,向振凡,陈克富,邹永翠,2012,种子成熟度对玉米幼苗生长的影响,作物杂志,5:96-99)
Zhang Y.H.,Wen C.Y.,2010,On cell membrane lipid peroxidation and changes of oxide resistance enzymes activities during the ripeness of Maize seeds,Xiangfan Xueyuan Xuebao(Journal of Xiangfan University),21(5):43-46(张艳红,文才艺,2010,玉米种子成熟过程中细胞膜脂过氧化及体内抗氧化酶活性变化的研究,襄樊学院学报,21(5):43-46)
Zhang Z.Y.,Zhu J.H.,Cheng Y.,Zhao X.L.,Li J.,Ru Z.,and Liu M.J.,2014,Seed vigor and its correlation with physiological characteristics of different maturity wheat seeds,Henan Nongye Kexue(Journal of Henan Agricultural Sciences),43(12):6-9(张自阳,朱俊华,程媛,赵新亮,李金,茹振,刘明久,2014,不同成熟度小麦种子活力及其与生理性状的相关性研究,河南农业科学,43(12):6-9)
Zheng Y.Y.,Yu X.M.,Suo W.L.,Niu Y.Z.,Deng S.B.,and Ma W.G.,2015,Study on the change and judgment of tobacco seed maturity,Zhiwu Fenlei Yu Ziyuan Xuebao(Plant Diversity and Resources),37(4):472-478(郑昀晔,禹晓梅,索文龙,牛永志,邓胜斌,马文广,2015,烟草种子成熟变化及判定方法研究,植物分类与资源学报,37(4):472-478)
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