1株耐多种重金属菌株的分离鉴定
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摘要
采集海南昌江矿区的土壤样品,通过分离与纯化,筛选出4株对金属铜(CuSO_4)具有较高耐受力的菌株,其中,菌株Cu-6还对铅(C_4H_6O_4Pb)和镉(CdCl_2)具有较高的耐受力。Cu-6属于阴沟肠杆菌(Enterobacter cloacae),其最适生长条件是:温度34℃,pH6.0,0.5%NaCl。菌株重金属耐受能力检测结果表明,菌株Cu-6能在800 mg·L~(-1) Cu~(2+),1 700 mg·L~(-1) Pb~(2+)和150 mg·L~(-1) Cd~(2+)的LB液体培养基中生长,3种离子同时存在时,菌株Cu-6仍能较好生长,显示出该菌株对环境有较强适应能力,可能对土壤重金属的污染具有修复潜力。
Heavy metals could affect the development of plants and microbial communities in soil. They can be transported into plants and animals by the food chain and may cause human diseases. In this context soil samples were collected in Changjiang mining area of Hainan Province, China, from which four copper-resistant strains were isolated. These strains were cultured in the LB medium containing different concentrations of heavy metals, and found to be resistant to copper. Moreover, the strain Cu-6 was observed to be resistant to plumbum(Pb) and cadmium(Cd) as well. Cu-6 was identified as Enterobacter cloacae, and had an optimal growth in the conditions of 34 ℃, pH6.0 and 0.5% NaCl. Cu-6 showed a good growth in the liquid LB medium containing 800 mg·L~(-1) CuSO_4·5 H_2O, 1 700 mg·L~(-1) C_4H_6O_4Pb·3 H_2O, 150 mg·L~(-1) CdCl_2·2.5 H_2O, or even mixture of these three metal ions, indicating that this strain should have a strong adaptation to the complex soil environment, and have a potential of soil improvement from the soil contaminated by heavy metals.
Heavy metals could affect the development of plants and microbial communities in soil. They can be transported into plants and animals by the food chain and may cause human diseases. In this context soil samples were collected in Changjiang mining area of Hainan Province, China, from which four copper-resistant strains were isolated. These strains were cultured in the LB medium containing different concentrations of heavy metals, and found to be resistant to copper. Moreover, the strain Cu-6 was observed to be resistant to plumbum(Pb) and cadmium(Cd) as well. Cu-6 was identified as Enterobacter cloacae, and had an optimal growth in the conditions of 34 ℃, pH6.0 and 0.5% NaCl. Cu-6 showed a good growth in the liquid LB medium containing 800 mg·L~(-1) CuSO_4·5 H_2O, 1 700 mg·L~(-1) C_4H_6O_4Pb·3 H_2O, 150 mg·L~(-1) CdCl_2·2.5 H_2O, or even mixture of these three metal ions, indicating that this strain should have a strong adaptation to the complex soil environment, and have a potential of soil improvement from the soil contaminated by heavy metals.
引文
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[3] 袁奎荣,候光汉,李公时,等.海南石碌铁矿的成因和富铁矿与构造的关系[J].中南矿冶学院学报,1977(3):26-43.
[4] HAN D,ZHANG X,TOMAR V,et al.Effects of heavy metal pollution of highway origin on soil nematode guilds in North Shenyang,China[J].Environmental Sciences,2009,21(2):193-198.
[5] FRIESL W,FRIEDL J,PLATZER K,et al.Remediation of contaminated agricultural soils near a former Pb/Zn smelter in Austria:Batch,pot and field experiments[J].Environmental Pollution,2006,144(1):40-50.
[6] MADEJóN P,DOMíNGUEZ M T,MURILLO J M.Pasture composition in a trace element-contaminated area:the particular case of Fe and Cd for grazing horses[J].Environmental monitoring and Assess,2012,184(5):2031-2043.
[7] SASTRE J,HERNáNDEZ E,RODRIGUEZ R,et al.Use of sorption and extraction tests to predict the dynamics of the interaction of trace elements in agricultural soils contaminated by a mine tailing accident[J].Science of the Total Environment,2004,329(1/2/3):261-281.
[8] GUO G L,ZHOU Q X,MA L Q.Availability and assessment of fixing additives for the in situ remediation of heavy metal contaminated soils:A review[J].Environmental Monitoring and Assessment,2006,116(1/2/3):513-528.
[9] 廖香俊.海南石碌铁矿矿区环境地球化学及环境治理研究[D].北京:中国地质大学,2008.
[10] AWOFOLU R,OKONKWO J,VANDERMENWE R,et al.A new approach to chemical modification protocols of Aspergillus niger and sorption of lead ion by fungal species[J].Electron J.Biotechnol,2006,9(4):340-348.
[11] BAPAT P,KUNDU S,WANGIKAR P.An optimized method for Aspergillus niger spore production on natural carrier substrates[J].Biotechnol Prog,2003,19(6):1683-1688.
[12] 康薇,罗闻真,瞿颖,等.一株耐铜细菌TLSB_2-K的鉴定及其铜吸附能力[J].湖北理工学院学报,2015,31(2):12-15.
[13] 王海鸥,钟广蓉,王立曼,等.一株耐铜细菌的鉴定及富集特性的研究[J].环境工程学报,2011,5(10):2380-2384.
[14] 李倩,张平,廖柏寒,等.一株耐Cd菌株的分离、鉴定及基本特性[J/OL].微生物学报:2017 (DOI:10.13343/j.cnki.wsxb.20170632)[2018-10-08].http://www.cnki.com.cn/Article/CJFDTotal-WSXB20180404001.htm.
[15] 吴海江,冯侠,郭红光,等.耐Cd细菌的筛选及富集作用研究[J].江西农业学报,2011,23(11):175-177,183.
[16] SETHURAMAN P,KUMAR M D.Bio-sorption kinetics of Cu (II) ions removal from aqueous solution using bacteria [J].Pakistan Journal of Biological Sciences,2011,14(5):327-335.
[17] KUERKE,MAJEWSKAM M.In vitro remobilization of Cd immobilized by fungal biomass[J].Geoderma,2004,122(2/3/4):235-246.
[18] MANASI,RAJESH V,KUMAR A S K,et al.Biosorption of cadmium using a novel bacterium isolated from an electronic industry effluent [J].Chemical Engineering Journal,2014,235(1):176-185.