玉米杂交种产量相关位点分析及优异等位变异挖掘
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摘要
采用NCⅡ设计,将13个玉米自交系配制42个杂交组合,选用120对SSR引物进行分子标记,采用基于回归的单标记分析方法,通过表型变异对标记变异的回归分析,筛选玉米产量相关等位变异位点,估计等位变异的效应和位点的基因型值,剖析杂种组合等位变异的位点效应。结果表明,筛选得到phi089、umc1142和umc1700等8个标记位点及其phi089-4、umc1142-5、umc1700-3、phi102-1、phi047-1、umc2317-2、bnlg1892-5和bnlg1352-3共8个优异增效等位变异位点;bnlg1352-3/5、phi089-4/7、umc2317-2/5、umc1142-4/5和bnlg1892-1/5共5个基因型值较大的位点为等位变异的优异杂合位点。构成杂合位点的2个等位变异位点各自的加性效应及其显性效应共同影响杂合位点的基因型值。HF12202、H2671、AMD1和MCO-1等材料携带着较多增效位点,属于优异亲本。
A total of 42 hybrid combinations from 13 maize inbred lines based on NCII design were analyzed with 120 SSR markers, then the yield-related loci was analyzed by single marker method based on regressive analysis. Then, 20 SSR markers were significantly associated with yield, and among them eight increasing alleles, that is,phi089-4, umc1142-5, umc1700-3, phi102-1, phi047-1, umc2317-2, bnlg1892-5, and bnlg1352-3, was identified.Moreover five excellent heterozygous loci, bnlg1352-3/5, phi089-4/7, umc2317-2/5, umc1142-4/5, bnlg1892-1/5,were also found to have relative higher yield genotype value, which resulted from the additive and dominant effects of two alleles within one locus. In addition, the maize inbred lines, HF12202, H2671, AMD1, and MCO-1, carrying more increasing yield-related alleles, were elite germplasm.
A total of 42 hybrid combinations from 13 maize inbred lines based on NCII design were analyzed with 120 SSR markers, then the yield-related loci was analyzed by single marker method based on regressive analysis. Then, 20 SSR markers were significantly associated with yield, and among them eight increasing alleles, that is,phi089-4, umc1142-5, umc1700-3, phi102-1, phi047-1, umc2317-2, bnlg1892-5, and bnlg1352-3, was identified.Moreover five excellent heterozygous loci, bnlg1352-3/5, phi089-4/7, umc2317-2/5, umc1142-4/5, bnlg1892-1/5,were also found to have relative higher yield genotype value, which resulted from the additive and dominant effects of two alleles within one locus. In addition, the maize inbred lines, HF12202, H2671, AMD1, and MCO-1, carrying more increasing yield-related alleles, were elite germplasm.
引文
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[16]逯晓萍,刘丹丹,王树彦,等.高丹草遗传效应与杂种表现预测模型[J].作物学报,2014,40(3):466-475.Lu X P,Liu D D,Wang S Y,et al.Sorghum-sudangrass genetic effect and hybrid performance prediction model[J].Acta Agronomica Sinica,2014,40(3):466-475.(in Chinese)
[17]杨胜先,牛远,李梦,等.栽培大豆农艺性状的关联分析及优异等位变异挖掘[J].中国农业科学,2014,47(20):3941-3952.Yang S X,Niu Y,Li M,et al.Association mapping of agronomic traits in soybean(Glycine max L.Merr.)and mining of novel alleles[J].Scientia Agricultura Sinica,2014,47(20):3941-3952.(in Chinese)
[18]Guo T,Yang N,Tong H,et al.Genetic basis of grain yield heterosis in an"immortalized F2"maize population[J].Theoretical&Applied Genetics,2014,127(10):2149-2158.
[19]向道权,曹海河,曹永国,等.玉米SSR遗传图谱的构建及产量性状基因定位[J].遗传学报,2001,28(8):778-784.Xiang D Q,Cao H H,Cao Y G,et al.Construction of a genetic map and location of quantitative trait loci for yield component trait in maize by SSR markers[J].Gnetics and Genomics,2001,28(8):778-784.(in Chinese)
[20]江培顺,张焕欣,吕香玲,等.玉米产量相关性状Meta-QTL及候选基因分析[J].作物学报,2013,39(6):969-978.Jiang P S,Zhang H X,LüX L,et al.Analysis of Meta-QTL and candidate genes related to yield components in maize[J].Acta Agronomica Sinica,2013,39(6):969-978.(in Chinese)
[21]杨加银,贺建波,王金社,等.大豆杂种产量相关的位点及等位变异分析[J].作物学报,2011,37(1):48-57.Yang J Y,He J B,Wang J S,et al.Soybean hybrid yield-related loci and allelic variation[J].Acta Agronomica Sinica,2011,37(1):48-57.(in Chinese)
[22]莫惠栋.世代平均数的遗传分析Ⅰ.加性-显性模型的基因效应估计[J].江苏农学院学报,1984(4):43-49.Mo H D.A genetic analysis of generation meansⅠ.estimates of genic effects for Additive-dominance mode[J].Journal of Jiangsu Agricultural College,1984(4):43-49.(in Chinese)
[23]Monir M M,Zhu J.Association analysis revealed importance of dominance effects on days to silk of maize nested association mapping(NAM)population[J].Journal of Zhejiang University(Agriculture and Life Sciences),2017,43(2):146-152.
[24]张学勇,童依平,游光霞,等.选择牵连效应分析:发掘重要基因的新思路[J].中国农业科学,2006,39(8):1526-1535.Zhang X Y,Tong Y P,You G X,et al.Hitchhiking effect mapping:a new approach for discovering agronomic important genes[J].Sci.Agric Sin.,2006,39:1526-1535.(in Chinese)
[25]ВеденеевГИ(Wang F D Trans).Genetic control of maize quantitative traits:III.Row number per ear and kernel number per row[J].Foreign Agron:Minor Cereals,1988(3):10-15.
[26]Xu Y B,Crouch J H.Assisted selection in plant breeding:from publications to practice[J].Crop Science,2008,48(2):391-407.
[2]姜献玲,田蜜,赵茜,等.基于SSR标记甜玉米遗传距离、配合力及杂种优势的关系[J].分子植物育种,2017,15(11):4518-4523.Jiang X L,Tian M,Zhao X,et al.Relationship between genetic distance,combining ability,and heterosis based SSR marker in sweet corn[J].Molecular Plant Breeding,2017,15(11):4518-4523.(in Chinese)
[3]姜涛,王丕武,张君.玉米m RNA差异显示与杂种优势关系的研究[J].玉米科学,2013,21(5):41-45.Jiao T,Wang P W,Zhang J,et al.Relationship between mrna differential gene expression and heterosis in maize[J].Journal of Maize Sciences,2013,21(5):41-45.(in Chinese)
[4]姚文华,罗黎明,汪燕芬,等.利用SSR标记和产量对27份玉米自交系进行杂种优势群划分[J].玉米科学,2009,17(1):54-58.Yao W H,Luo L M,Wang Y F,et al.Heterotic grouping of 27maize inbred lines divided by SSR markers and yields[J].Journal of Maize Sciences,2009,17(1):54-58.(in Chinese)
[5]张华丽,陈晓阳,黄建中,等.中国两系杂交水稻光温敏核不育基因的鉴定与演化分析[J].中国农业科学,2015,48(1):1-9.Zhang H L,Chen X Y,Huang J Z,et al.Identification and Transition Analysis of Photo-/Thermo-Sensitive genic male sterile genes in Two-Line hybrid rice in china[J].Scientia Agricultura Sinica,2015,48(1):1-9.(in Chinese)
[6]寇程,高欣,李立群,等.小麦粒重基因Ta GW2-6A等位变异的组成分析及育种选择[J].作物学报,2015,41(11):1640-1647.Kou C,Gao X,Li L Q,et al.Composition and selection of Ta GW2-6A alleles for wheat kernel weight[J].Acta Agronomica Sinica,2015,41(11):1640-1647.(in Chinese)
[7]Li Q,Lin L,Yang X,et al.Relationship,evolutionary fate and function of two maize co-orthologs of rice GW2 associated with kernel size and weight[J].BMC Plant Biology,2010,10(1):143.
[8]刁西洲,王红武,胡小娇,等.玉米穗部性状遗传和杂种优势分析[J].作物杂志,2015(4):36-40.Diao X Z,Wang H W,Hu X J,et al.Genetic and heterosis analysis of maize ear characteristics[J].Crops,2015(4):36-40.(in Chinese)
[9]He G M,Luo X J,Tian F,et al.Haplotype Variation in structure and expression of a gene cluster that is associated with a quantitative trait locus for improved yield in rice[J].Genome Research,2006,16:618-626.
[10]Huang X,Yang S,Gong J,et al.Genomic architecture of heterosis for yield traits in rice[J].Nature,2016,537:629-633.
[11]张福彦,陈锋,程仲杰,等.小麦Ta Lox-B等位变异对脂肪氧化酶活性和面粉色泽的影响[J].中国农业科学,2017,50(8):1370-1377.Zhang F Y,Chen F,Cheng Z J,Effects of Ta Lox-B alleles on lipoxygenase activity and flour color in wheats[J].Scientia Agricultura Sinica.,2017,50(8):1370-1377.(in Chinese)
[12]Lu X P,Yun J F,Gao C P.QTL Analysis of economica11y important traits in sorghum bicolor×S.sudanense hybrid,canadian journal of p1ant science[J].P1ant Sci.,2011(91):81-90.
[13]Huo D,Qiang N,Shen X,et al.QTL Mapping of kernel NumberRelated traits and validation of one major qtl for ear length in maize[J].Plos One,2016,11(5):0155506.
[14]刘晓阳,卫晓轶,陈浩,等.玉米主要植株性状的杂种优势位点分析[J].中国农业科学,2017,50(7):1179-1188.Liu X Y,Wei X Y,Chen H,et al.Identification of heterotic loci for plant traits using chromosomal segment substitution lines test population in maize[J].Scientia Agricultura Sinica,2017,50(7):1179-1188.(in Chinese)
[15]Riedelsheimer C,Czedikeysenberg A,Grieder C,et al.Genomic and metabolic prediction of complex heterotic traits in hybrid maize[J].Nature Genetics,2012,44(2):217-20.
[16]逯晓萍,刘丹丹,王树彦,等.高丹草遗传效应与杂种表现预测模型[J].作物学报,2014,40(3):466-475.Lu X P,Liu D D,Wang S Y,et al.Sorghum-sudangrass genetic effect and hybrid performance prediction model[J].Acta Agronomica Sinica,2014,40(3):466-475.(in Chinese)
[17]杨胜先,牛远,李梦,等.栽培大豆农艺性状的关联分析及优异等位变异挖掘[J].中国农业科学,2014,47(20):3941-3952.Yang S X,Niu Y,Li M,et al.Association mapping of agronomic traits in soybean(Glycine max L.Merr.)and mining of novel alleles[J].Scientia Agricultura Sinica,2014,47(20):3941-3952.(in Chinese)
[18]Guo T,Yang N,Tong H,et al.Genetic basis of grain yield heterosis in an"immortalized F2"maize population[J].Theoretical&Applied Genetics,2014,127(10):2149-2158.
[19]向道权,曹海河,曹永国,等.玉米SSR遗传图谱的构建及产量性状基因定位[J].遗传学报,2001,28(8):778-784.Xiang D Q,Cao H H,Cao Y G,et al.Construction of a genetic map and location of quantitative trait loci for yield component trait in maize by SSR markers[J].Gnetics and Genomics,2001,28(8):778-784.(in Chinese)
[20]江培顺,张焕欣,吕香玲,等.玉米产量相关性状Meta-QTL及候选基因分析[J].作物学报,2013,39(6):969-978.Jiang P S,Zhang H X,LüX L,et al.Analysis of Meta-QTL and candidate genes related to yield components in maize[J].Acta Agronomica Sinica,2013,39(6):969-978.(in Chinese)
[21]杨加银,贺建波,王金社,等.大豆杂种产量相关的位点及等位变异分析[J].作物学报,2011,37(1):48-57.Yang J Y,He J B,Wang J S,et al.Soybean hybrid yield-related loci and allelic variation[J].Acta Agronomica Sinica,2011,37(1):48-57.(in Chinese)
[22]莫惠栋.世代平均数的遗传分析Ⅰ.加性-显性模型的基因效应估计[J].江苏农学院学报,1984(4):43-49.Mo H D.A genetic analysis of generation meansⅠ.estimates of genic effects for Additive-dominance mode[J].Journal of Jiangsu Agricultural College,1984(4):43-49.(in Chinese)
[23]Monir M M,Zhu J.Association analysis revealed importance of dominance effects on days to silk of maize nested association mapping(NAM)population[J].Journal of Zhejiang University(Agriculture and Life Sciences),2017,43(2):146-152.
[24]张学勇,童依平,游光霞,等.选择牵连效应分析:发掘重要基因的新思路[J].中国农业科学,2006,39(8):1526-1535.Zhang X Y,Tong Y P,You G X,et al.Hitchhiking effect mapping:a new approach for discovering agronomic important genes[J].Sci.Agric Sin.,2006,39:1526-1535.(in Chinese)
[25]ВеденеевГИ(Wang F D Trans).Genetic control of maize quantitative traits:III.Row number per ear and kernel number per row[J].Foreign Agron:Minor Cereals,1988(3):10-15.
[26]Xu Y B,Crouch J H.Assisted selection in plant breeding:from publications to practice[J].Crop Science,2008,48(2):391-407.