尿脱落细胞琼脂石蜡双包埋连续切片法
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摘要
目的研究设计一种尿脱落细胞连续切片方法。方法收集尿液标本离心沉淀,先用琼脂将沉淀物包埋成团块后进行脱水、透明、浸蜡,再用石蜡包埋,然后进行石蜡连续切片(3~4μm)、苏木精-伊红(HE)染色。结果镜下细胞集中、结构清晰、厚薄均匀,细胞量明显比涂片法丰富,部分可见组织结构;HE染色核质对比好,色彩鲜艳明亮。结论尿脱落细胞琼脂石蜡双包埋连续切片法克服了涂片法的不足,设备简单,操作简便,宜于普及推广。
Objective To study and design a new consecutive section method of urine exfoliated cells.Methods The centrifugated urine samples were collected.The precipitates were embedded with agar,and then the dehydration,transparency and waxdip have been done.Finally,the specimens were blocked in paraffin,and were consecutively cut into 3-4μm sections.All Sections were stained with HE.Results Under the microscope,more urine exfoliated cells were observed in the consecutive sections than those in the smears,showed concentrated cells,clear structure and homogeneous thickness,and tissue structures were partially visible.The nucleoplasm was identified easily in the consecutive sections stained with HE with bright colour.ConclusionThe consecutive section method of agar-paraffin double-embedded urine exfoliated cells overcomes the shortcomings of the smear method,and needs simple equipment with easier operation,which is suitable for popularization in primary medical institutions.
Objective To study and design a new consecutive section method of urine exfoliated cells.Methods The centrifugated urine samples were collected.The precipitates were embedded with agar,and then the dehydration,transparency and waxdip have been done.Finally,the specimens were blocked in paraffin,and were consecutively cut into 3-4μm sections.All Sections were stained with HE.Results Under the microscope,more urine exfoliated cells were observed in the consecutive sections than those in the smears,showed concentrated cells,clear structure and homogeneous thickness,and tissue structures were partially visible.The nucleoplasm was identified easily in the consecutive sections stained with HE with bright colour.ConclusionThe consecutive section method of agar-paraffin double-embedded urine exfoliated cells overcomes the shortcomings of the smear method,and needs simple equipment with easier operation,which is suitable for popularization in primary medical institutions.
引文
[1]MONTEIRO-REIS S,LECA L,ALMEIDA M,et al.Accurate detection of upper tract urothelial carcinoma in tissue and urine by means of quantitative GDF15,TMEFF2and VIM promoter methylation[J].Eur J Cancer,2014,50(1):226-233.
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[8]何非平.吡柔比星不同灌注方法对膀胱癌术后复发的影响[J].实用药物与临床,2014,17(1):50-52.
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[11]TSCHIRDEWAHN S,VOM DORP F.Diagnostics of nonmuscle-invasive urothelial cell carcinoma of the bladder[J].Urologe A,2015,54(4):480-483.
[12]MALKHASIANKA,PETROVSV,UL′IANIN M I,et al.Use of fluorescent in situ hybridization in the cytogenetic diagnosis of urinary bladder urothelial carcinoma[J].Vopr Onkol,2011,57(4):462-469.
[13]XU C,ZENG Q,HOU J,et al.Utility of a modality combining FISH and cytology in upper tract urothelial carcinoma detection in voided urine samples of Chinese patients[J].Urology,2011,77(3):636-641.
[14]JIA X,YU Q,ZHANG Z H,et al.Targeting bladder tumor cells in voided urine of Chinese patients with FITC-CSNRDARRC peptide ligand[J].Onco Targets Ther,2012(5):85-90.
[15]MILICIC V,PRVULOVIC I,PANDA N,et al.Diagnostic value of cytology of voided urine[J].Coil Antropol,2014,38(2):617-620.
[16]RAGHAVAN D,SHIPLEY W U,GARNICK M B,et al.Biology and management of bladder cancer[J].N Engl J Med,1990,322(16):1129-1138.
[17]PROCTOR I,STOEBER K,WILLIAMS G H.Biomarkers in bladder cancer[J].Histopathology,2010,57(1):1-13.
[18]URQUIDI V,ROSSER C J,GOODISON S.Multiplex urinary tests for bladder cancer diagnosis[J].Eur Med J Urol,2013(1):70-73.
[2]ALTIERI V M,CASTELLUCCI R,PALUMBO P,et al.Recurrence and progression in non-muscle-invasive bladder cancer using EORTC risk tables[J].Urol Int,2012,89(1):61-66.
[3]韩苏军,张思维,陈万青,等.中国膀胱癌发病现状及流行趋势分析[J].癌症进展,2013,11(1):89-95.
[4]KIM S Y,MYUNG S J.Optical molecular imaging for diagnosingintestinal diseases[J].Clinic Endosc,2013,46(6):620-626.
[5]那彦群.中国泌尿外科疾病诊断治疗指南[M].北京:人民卫生出版社,2013:21-28.
[6]宋正尧,梁朝朝,江长琴,等.FISH技术在中国人膀胱尿路上皮癌诊断及复发监测中应用的研究[J].临床泌尿外科杂志,2011,26(11):823-826.
[7]沈瑞存,邵可可.新辅助化疗在经尿道膀胱肿瘤电切术中的应用[J].实用药物与临床,2014,17(7):875-878.
[8]何非平.吡柔比星不同灌注方法对膀胱癌术后复发的影响[J].实用药物与临床,2014,17(1):50-52.
[9]RAITANEN M P,KAASINEN E,LUKKARINEN O,et al.Analysis of false-positive BTA STAT test results in patients followed up for bladder cancer[J].Urology,2001,57(4):680-684.
[10]INOUE T,NASU Y,TSUSHIMA T,et al.Chromosomal numerical aberrations of exfoliated cells in the urine detected by fluorescence in situ hybridization:clinical implication for the detection of bladder cancer[J].Urol Res,2000,28(1):57-61.
[11]TSCHIRDEWAHN S,VOM DORP F.Diagnostics of nonmuscle-invasive urothelial cell carcinoma of the bladder[J].Urologe A,2015,54(4):480-483.
[12]MALKHASIANKA,PETROVSV,UL′IANIN M I,et al.Use of fluorescent in situ hybridization in the cytogenetic diagnosis of urinary bladder urothelial carcinoma[J].Vopr Onkol,2011,57(4):462-469.
[13]XU C,ZENG Q,HOU J,et al.Utility of a modality combining FISH and cytology in upper tract urothelial carcinoma detection in voided urine samples of Chinese patients[J].Urology,2011,77(3):636-641.
[14]JIA X,YU Q,ZHANG Z H,et al.Targeting bladder tumor cells in voided urine of Chinese patients with FITC-CSNRDARRC peptide ligand[J].Onco Targets Ther,2012(5):85-90.
[15]MILICIC V,PRVULOVIC I,PANDA N,et al.Diagnostic value of cytology of voided urine[J].Coil Antropol,2014,38(2):617-620.
[16]RAGHAVAN D,SHIPLEY W U,GARNICK M B,et al.Biology and management of bladder cancer[J].N Engl J Med,1990,322(16):1129-1138.
[17]PROCTOR I,STOEBER K,WILLIAMS G H.Biomarkers in bladder cancer[J].Histopathology,2010,57(1):1-13.
[18]URQUIDI V,ROSSER C J,GOODISON S.Multiplex urinary tests for bladder cancer diagnosis[J].Eur Med J Urol,2013(1):70-73.