基于电化学还原氧化石墨烯的电化学DNA生物传感器

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英文篇名：Electrochemical DNA biosensors based on electrochemical reduced graphene oxide 作者：朱济锋 ; 黎学思 ; 周大明 ; 于乐泳 ; 王国东 ; Chaker ; Tlili 英文作者：ZHU Jifeng;LI Xuesi;ZHOU Daming;YU Leyong;WANG Guodong;Chaker Tlili;School of Electrical Engineering and Automation,Henan Polytechnic University;Research Center for Precision Medicine and Single Molecule Diagnostics,Chongqing Institute of Green and Intelligent Technology,Chinese Academy of Sciences;School of Physics and Electronic Information Engineering,Henan Polytechnic University; 关键词：还原氧化石墨烯 ; 氨基基团 ; π-π键 ; DNA生物传感器 英文关键词：reduced graphene oxide;;amino group;;π-π stacking;;DNA biosensors 中文刊名：CGQJ 英文刊名：Transducer and Microsystem Technologies 机构：河南理工大学电气工程与自动化学院;中国科学院重庆绿色智能技术研究院精准医疗单分子诊断中心;河南理工大学物理与电子信息学院; 出版日期：2019-06-10 出版单位：传感器与微系统 年：2019 期：v.38;No.328 语种：中文; 页：CGQJ201906021 页数：4 CN：06 ISSN：23-1537/TN 分类号：81-84

摘要

使用还原氧化石墨烯(rGO)制备一种简单、快速和可重复方法构建DNA生物传感器。将带负电的氧化石墨烯(GO)与半胱氨酸上带正电的氨基基团通过静电作用相互吸附,用线性扫描伏安法(LSV)电化学还原电极表面吸附的GO。将二茂铁标记的DNA(Fc-DNA)探针固定到r GO表面,成功构建DNA传感器。传感器的制备过程使用循环伏安法和拉曼光谱表征。通过杂化前后DNA传感器所展现出方波信号峰电流的差异,实现对目标DNA的定量检测。实验结果表明:目标DNA浓度在1. 0×10~(-13)～1. 0×10~(-6)mol/L范围内,峰电流变化与目标DNA浓度呈线性关系,线性相关系数为0. 981,检测限是2. 0×10~(-13)mol/L (S/N=3)。
        A simple,facile and reproducible approach is used for fabrication of DNA biosensors based on reduced graphene oxide( r GO). The graphene oxide( GO) nanosheets are assembled onto cysteamine-modified gold electrodes via electrostatic interaction between the positively charged amino group of cysteamine and the negatively charged groups present in GO,followed by electro-reduction through linear sweep voltammetry( LSV) and functionalization with ferrocene-labeled DNA probe. The stepwise assembly of the DNA( Fc-DNA) biosensor is characterized with cyclic voltammetry and Raman spectroscopy. The target DNA can be quantitatively detected based on the variation of square wave voltammetry( SWV) signal of the biosensor before and after hybridization with target DNA. The peak current variation has a linear relationship with the target DNA concentration in the range of 1. 0 × 10~(-13)～ 1. 0 × 10~(-6) M,with linearly dependent coefficient of 0. 981 and the detection limit( LOD)is 2. 0 × 10~(-13) mol/L( S/N = 3).

引文

[1]马丽,白燕,刘仲明,等.电化学DNA传感器研究进展[J].传感器与微系统,2002,21(3):58-60.
    [2] LIN L,HARRIS J W,THOMPSON H G,et al. Surface plasmon resonance-based sensors to identify cis-regulatory elements[J].Analytical Chemistry,2004,76(22):6555-6559.
    [3] CHOMEAN S,POTIPITAK T,PROMPTMAS C,et al. Quartz crystal microbalance-based biosensor for the detection ofα-thalassemia 1(SEA deletion)[J]. Clinical Chemistry&Laboratory Medicine,2010,48(9):1247-1254.
    [4] LIN Y,LI Z,CHEN Z,et al. Mesoporous silica-encapsulated gold nanoparticles as artificial enzymes for self-activated cascade catalysis[J]. Biomaterials,2013,34(11):2600-2610.
    [5] SHI S,CHEN F,EHLERDING E B,et al. Surface engineering of graphene-based nanomaterials for biomedical applications[J].Bioconjug Chem,2014,25(9):1609-1619.
    [6] LIU B,LIU J. DNA adsorption by indium tin oxide nanoparticles[J]. Langmuir the ACS Journal of Surfaces&Colloids,2015,31(1):371-377.
    [7] VARGHESE N,MOGERA U,GOVINDARAJ A,et al. Binding of DNA nucleobases and nucleosides with graphene[J]. Chemphyschem,2010,10(1):206-210.
    [8] TLILI C,SOKULLU E,SAFAVIEH M,et al. Bacteria screening,viability,and confirmation assays using bacteriophage-impedimetric/loop-mediated isothermal amplification dual-response biosensors[J]. Analytical Chemistry,2013,85(10):4893-4901.
    [9] WANG D W,LI F,ZHAO J,et al. Fabrication of graphene/polyaniline composite paper via in situ anodic electropolymerization for high-performance flexible electrode[J]. ACS Nano,2009,3(7):1745-1752.
    [10] PING J,WANG Y,FAN K,et al. Direct electrochemical reduction of graphene oxide on ionic liquid doped screen-printed electrode and its electrochemical biosensing application[J]. Biosensors&Bioelectronics,2011,28(1):204-209.
    [11] SHAO Y,WANG J,ENGELHARD M,et al. Facile and controllable electrochemical reduction of graphene oxide and its applications[J]. Journal of Materials Chemistry,2010,20(4):743-748.
    [12] RAJ M A,JOHN S A. Graphene layer modified glassy carbon electrode for the determination of norepinephrine and theophylline in pharmaceutical formulations[J]. Analytical Methods,2014,6(7):2181-2188.
    [13] ELSHAFEY R,TLILI C,ABULROB A,et al. Label-free impedimetric immunosensor for ultrasensitive detection of cancer marker murine double minute 2 in brain tissue[J]. Biosensors&Bioelectronics,2013,39(1):220-225.
    [14] YUNG W K C,LI G,HAI M L,et al. Eye-friendly reduced graphene oxide circuits with nonlinear optical transparency on flexible poly(ethylene terephthalate)substrates[J]. Journal of Materials Chemistry C,2015,3(43):11294-11299.
    [15] JANG H,KIM Y K,KWON H M,et al. A graphene-based platform for the assay of duplex-DNA unwinding by helicase[J]. Angewandte Chemie International Edition,2010,49(33):5703-5707.
    [16] TLILI C,JAFFREZIC-RENAULT N J,MARTELET C,et al.Direct electrochemical probing of DNA hybridization on oligonucleotide-functionalized polypyrrole[J]. Materials Science&Engineering C,2008,28(5-6):848-854.
    [17] DIOUANI M F,OUERGHI O,REFAI A,et al. Detection of ESAT-6by a label free miniature immuno-electrochemical biosensor as a diagnostic tool for tuberculosis[J]. Materials Science&Engineering C,2016,74:465-470.