不同日龄五指山猪胰腺胰岛的组织学及免疫组织化学研究
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摘要
猪胰岛是治疗人类Ⅰ型糖尿病的理想异种供体。为给胰岛的异种移植提供形态学基础资料,分别以出生后1~10、40、120和180d的五指山猪的胰腺为试验材料,对五指山猪出生后不同日龄胰岛的组织学及组织化学特性进行了研究。结果表明:五指山猪胰腺在出生后第1~10d未生成典型胰岛,在第40、120、180d出现典型胰岛,随日龄增长胰高血糖素免疫反应细胞呈逐渐增加的趋势;出生后不同日龄五指山猪胰腺均具有胰岛素免疫反应阳性细胞、胰高血糖素免疫反应阳性细胞和生长抑素免疫反应阳性细胞,这些细胞的分布面积随日龄增加呈增大的趋势。
Porcine islets are regarded as an ideal source of xenogeneic donors in the treatment of human type I diabetes.The pancreas of Wuzhishan pig,which was 1-10 days,40 days,120 days,180 days ages,was used as experimental material.The histological and histochemical characteristics of islets of Wuzhishan pig at different ages after birth were observed.The results showed that there were no typical islets in the pancreas of Wuzhishan pigs 1-10 dafter birth,but the typical islets and glucagon immunoreactive cells could be seen on 180 dafter birth.There were insulin immunoreactive cells,glucagon immunoreactive cells and somatostatin immunoreactive cells in the pancreas of Wuzhishan pigs at different postnatal days,and the distribution area of these cells increased with the age of day.
Porcine islets are regarded as an ideal source of xenogeneic donors in the treatment of human type I diabetes.The pancreas of Wuzhishan pig,which was 1-10 days,40 days,120 days,180 days ages,was used as experimental material.The histological and histochemical characteristics of islets of Wuzhishan pig at different ages after birth were observed.The results showed that there were no typical islets in the pancreas of Wuzhishan pigs 1-10 dafter birth,but the typical islets and glucagon immunoreactive cells could be seen on 180 dafter birth.There were insulin immunoreactive cells,glucagon immunoreactive cells and somatostatin immunoreactive cells in the pancreas of Wuzhishan pigs at different postnatal days,and the distribution area of these cells increased with the age of day.
引文
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[19]刁红艳,张译文,方鹤,等.胰岛细胞分化与胚体发育的相关性研究[J].四川解剖学杂志,2010(02):4-6.
[2]扈世航.糖尿病足防治与护理的探讨[J].赤峰学院学报(自然科学版),2017,33(11):160-161.
[3]赵燕英,陈正望,王建和.Aglycin在小鼠组织中的定位[J].西南民族大学学报(自然科学版),2011,37(4):582-585.
[4]孙国鹏,尹国安,张艳芳.低剂量链脉佐菌素诱导迟发性I型糖尿病小鼠模型[J].黑龙江八一农垦大学学报(自然科学版),2013,25(3):30-33.
[5]蒋小峰,钱滔来,向广阳,等.五指山小型猪胰岛分离和纯化的初步研究[J].临床医学工程,2011,18(9):1243-1347.
[6]Svenstrup K,Skau M,Pakkenberg B,et al.Postnatal development of beta-cells in rats.Proposed explanatory model[J].APMIS,2002,110(5):372-378.
[7]韩玉静,马群山.关于减少羊胚胎移植术后感染方法的建立[J].黑龙江八一农垦大学学报,2009,21(2):61-63.
[8]刘志科,潘耀谦,张秋雨.犬瘟热病例消化系统的病理形态学研究[J]河南科技学院学报(自然科学版),2017,45(5):48-53.
[9]靳二辉.近交系五指山小型猪解剖组织学研究及内源性反转录病毒(PERV)的检测[D].武汉:华中农业大学,2008.
[10]章雷,顾健,李佳川,等.葛根黄连有效组分配伍对STZ复合高脂大鼠2型糖尿病模型的防治作用[J].西南民族大学学报(自然科学版),2016,42(1):61-66.
[11]宋振顺,顾克菊.成人胰腺干细胞转分化为胰岛细胞过程中的形态学观察[J].中华外科杂志,2002,40(11):807-810.
[12]Finegood D T,Scaglia L,Bonner-Weir S.Dynamics of beta-cell mass in the growing rat pancreas.Estimation with a simple mathematical model[J].Diabetes J,1995,44(3):249-256.
[13]Bock T,Kyhnel A,Pakkenberg B,et al.The postnatal growth of the beta-cell mass in pigs[J].J Endocrinol,2003,1799(2):245–252.
[14]丁后友,丁圣刚,冠心病合并糖代谢异常患者脂蛋白的变化及其临床意义[J].赤峰学院学报(自然科学版),2016,32(7):201-203.
[15]Weinberg D S,Ruggeri B,Barber M T,et al.Cholecystokinin A and B receptors are differentially expressed in normal pancreas and pancreatic adenocarcinoma[J].J Clin Invest J,1997,100(3):597-603.
[16]Tari A,Kamiyazu T,Yonie Y,et al.Role of gastrin/CCK-B receptor in the regulation of gastrin acid secretion in rat[J].Digest Dis Sci J,1997,42(9):1901-1907.
[17]魏刚才,郑爱武,谢红兵,等.早期饲喂对肉鸡生长性能和肠道消化酶的影响[J],2014,42(1):38-42.
[18]Buggenhout A,Hoang A,Hut F,et al.Pediatric en bloc dual kidney-pancreas transplantation into an adult recipient:a simplified technique.Benefits of the en bloc kidney-pancreas transplantation technique in pediatric donors[J].Am J Transplant J,2004,4(4):663-665.
[19]刁红艳,张译文,方鹤,等.胰岛细胞分化与胚体发育的相关性研究[J].四川解剖学杂志,2010(02):4-6.
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