支气管哮喘患儿外周血T细胞亚群和血清趋化因子及酶类多肽水平
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摘要
目的:探讨支气管哮喘患儿外周血T细胞亚群、血清趋化因子及酶类多肽水平变化及意义。方法:60例支气管哮喘患儿为研究组,另选取同期体检健康儿童50例为对照组,采集2组受试者清晨空腹静脉血3 mL,使用流式细胞仪检测外周血CD+4T细胞亚群中辅助性T淋巴细胞(Th1、Th2、Th17)及调节性T细胞(Treg),计算Th1/Th2、Th17/Treg比值;另采集清晨空腹静脉血3 mL,采用酶联免疫吸附法(ELISA)检测血清白细胞介素-2(IL-2)、IL-4、IL-6、IL-17、干扰素-γ(IFN-γ)、嗜酸性粒细胞趋化因子(Eotaxin)、巨噬细胞衍生趋化因子(MDC)、单核细胞趋化蛋白-1(MCP-1)、单核细胞趋化蛋白-4(MCP-4)、基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)、组织蛋白酶K(Cat K)、组织金属蛋白酶抑制因子-1(TIMP-1)水平。结果:研究组外周血Th1、Treg、Th1/Th2低于对照组,Th2、Th17、Th17/Treg高于对照组(P<0.05);研究组血清IL-2、IFN-γ水平低于对照组,IL-4、IL-6、IL-17水平高于对照组(P<0.05);研究组血清Eotaxin、MDC、MCP-1、MCP-4水平高于对照组(P<0.05),研究组血清MMP-1、MMP-2、MMP-9、Cat K水平高于对照组、TIMP-1水平低于对照组(P<0.05)。结论:支气管哮喘患儿发病与外周血T淋巴细胞亚群、酶类多肽、趋化因子异常表达有关。
Objective:To explore the changes and significance of T cell subsets in peripheral blood,serum chemokines and enzyme peptides in children with bronchial asthma.Methods:60 children with bronchial asthma were selected as study group and 50 healthy children as control group.Fasting venous blood was collected from two groups for 3 mL in the morning.Auxiliary T lymphocytes(Th1、Th2、Th17) and regulatory T cells(Treg) in Peripheral Blood CD4+T Cell subsets were detected by flow Cytometry to calculate the ratio of Th1/Th2 and Th17/Treg.After 3 mL of fasting venous blood was collected in the morning,serum Interleukin(IL)-2(IL-2),IL-4,IL-6,IL-17,IFN-γ,Eotaxin,MDC,MCP-1,MCP-4,MMP-1,MMP-2,MMP-9,Cat K and TIMP-1 were detected by Enzyme-linked Immunosorbent Assay(ELISA).Results:Th1,Treg,and Th1/Th2 in the study group were significantly lower than that in the control group,while Th2,Th17,and Th17/Treg were significantly higher than that in the control group(P<0.05).IL-2 and IFN-γ in the study group were significantly lower than that in the control group,while IL-4,IL-6,and IL-17 were significantly higher than that in the control group(P<0.05).Eotaxin,MDC,MCP-1,and MCP-4 in the study group were significantly higher than that in the control group(P<0.05).MMP-1,MMP-2,MMP-9,and Cat K in the study group were significantly higher than that in the control group,while TIMP-1 was significantly lower than that in the control group(P<0.05).Conclusion:The pathogenesis of bronchial asthma is related to the abnormal expression of T lymphocyte subsets,enzyme peptides and chemokines in peripheral blood.
Objective:To explore the changes and significance of T cell subsets in peripheral blood,serum chemokines and enzyme peptides in children with bronchial asthma.Methods:60 children with bronchial asthma were selected as study group and 50 healthy children as control group.Fasting venous blood was collected from two groups for 3 mL in the morning.Auxiliary T lymphocytes(Th1、Th2、Th17) and regulatory T cells(Treg) in Peripheral Blood CD4+T Cell subsets were detected by flow Cytometry to calculate the ratio of Th1/Th2 and Th17/Treg.After 3 mL of fasting venous blood was collected in the morning,serum Interleukin(IL)-2(IL-2),IL-4,IL-6,IL-17,IFN-γ,Eotaxin,MDC,MCP-1,MCP-4,MMP-1,MMP-2,MMP-9,Cat K and TIMP-1 were detected by Enzyme-linked Immunosorbent Assay(ELISA).Results:Th1,Treg,and Th1/Th2 in the study group were significantly lower than that in the control group,while Th2,Th17,and Th17/Treg were significantly higher than that in the control group(P<0.05).IL-2 and IFN-γ in the study group were significantly lower than that in the control group,while IL-4,IL-6,and IL-17 were significantly higher than that in the control group(P<0.05).Eotaxin,MDC,MCP-1,and MCP-4 in the study group were significantly higher than that in the control group(P<0.05).MMP-1,MMP-2,MMP-9,and Cat K in the study group were significantly higher than that in the control group,while TIMP-1 was significantly lower than that in the control group(P<0.05).Conclusion:The pathogenesis of bronchial asthma is related to the abnormal expression of T lymphocyte subsets,enzyme peptides and chemokines in peripheral blood.
引文
[1]屈蕾蕾,达春和,刘丽君,等.支气管激发试验预测支气管哮喘患者治疗的远期预后分级[J].中国全科医学,2015,18(8):960-962.
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[8]郭惠环,林少玲.孟鲁司特对儿童支气管哮喘患者T淋巴细胞亚群检测的影响[J].黑龙江医学,2015,39(8):935-936.
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[10]缪华,刘晓蓉.不同呼吸道病毒感染与小儿哮喘发病及细胞因子!淋巴细胞亚群含量的关系[J].海南医学院学报,2017,23(8):1088-1091.
[11]袁颖志,贺宇彤,吴成,等.Th1/Th2/Th17/Treg对鉴别儿童支气管哮喘与单纯性肺炎的意义[J].河北医药,2016,38(9):1285-1288.
[12]郝珉,宋文,路苓.孟鲁司特钠对支气管哮喘患儿Th17/Treg细胞平衡及肺功能的影响[J].当代医学,2017,23(21):11-14.
[13]邵美琪,吴世满,刘娟,等.骨化三醇对支气管哮喘患者外周血Th17/Treg表达的调节作用[J].中华临床医师杂志(电子版),2015,9(19):34-38.
[14]兰芙蓉,陈文燕,王明章.儿童支气管哮喘患者血清骨桥蛋白和嗜酸细胞趋化因子表达及临床意义[J].临床肺科杂志,2017,22(5):877-879.
[15]吴丽梅,黄丽素,徐兰飞.寒喘祖帕颗粒联合沙丁胺醇雾化吸入治疗小儿支气管哮喘的疗效及对血清MPO、PMN、MCP-4的影响[J].中国生化药物杂志,2017,37(4):87-90.
[16]谢中勇,陈伟明,张伟忠,等.支气管哮喘患儿血清中酶类多肽、趋化因子水平及外周血免疫细胞含量的评估[J].海南医学院学报,2015,21(8):1103-1105.
[17]杨振明,吴丙美,寇永妹,等.舒利迭对儿童哮喘的治疗作用及对血清中MMP-2、MMP-3和MMP-9的影响[J].现代中西医结合杂志,2015,24(11):1187-1188.
[18]谢泽周.学龄期哮喘患儿治疗前后血清TIMP-1、IL-10、hs-CRP及TH的变化[J].罕少疾病杂志,2016,23(5):23-25.
[2]肖天赐.定喘汤联合西医常规治疗对过敏性支气管哮喘患者外周血单个核细胞Th1/Th2及黏膜免疫功能的影响[J].河北中医,2017,39(7):1048-1052.
[3]中华医学会儿科学分会呼吸学组《中华儿科杂志》编辑委员会.儿童支气管哮喘诊断与防治指南(2016版)[J].中华儿科杂志,2016,54(3):167-181.
[4]高志刚,袁永红,詹建华.沙丁胺醇气雾剂联合硫酸镁对小儿哮喘IL-2、IL-4、IL-5、IFN-γ及T淋巴细胞亚群的影响[J].中国生化药物杂志,2015,38(8):110-112.
[5]黄伟强,李惠,袁梅,等.支气管哮喘患者外周血T细胞亚群Th1、Th2及其相关细胞因子水平变化及意义[J].山东医药,2015,12(20):40-41.
[6]袁颖志,贺宇彤,吴成,等.Th1/Th2/Th17/Treg对鉴别儿童支气管哮喘与单纯性肺炎的意义[J].河北医药,2016,38(9):1285-1288.
[7]孟珊珊,于金燕,闫冰迪,等.哮喘患者调节性T细胞对Th1和Th2细胞的影响[J].中华实用诊断与治疗杂志,2015,29(8):742-744.
[8]郭惠环,林少玲.孟鲁司特对儿童支气管哮喘患者T淋巴细胞亚群检测的影响[J].黑龙江医学,2015,39(8):935-936.
[9]BIELINSKA A U,O’KONEK J J,JANCZAK K W,et al.immunomodulation of TH2 biased immunity with mucosal administration of nanoemulsion adjuvant[J].Vaccine,2016,34(34):4017-4024.
[10]缪华,刘晓蓉.不同呼吸道病毒感染与小儿哮喘发病及细胞因子!淋巴细胞亚群含量的关系[J].海南医学院学报,2017,23(8):1088-1091.
[11]袁颖志,贺宇彤,吴成,等.Th1/Th2/Th17/Treg对鉴别儿童支气管哮喘与单纯性肺炎的意义[J].河北医药,2016,38(9):1285-1288.
[12]郝珉,宋文,路苓.孟鲁司特钠对支气管哮喘患儿Th17/Treg细胞平衡及肺功能的影响[J].当代医学,2017,23(21):11-14.
[13]邵美琪,吴世满,刘娟,等.骨化三醇对支气管哮喘患者外周血Th17/Treg表达的调节作用[J].中华临床医师杂志(电子版),2015,9(19):34-38.
[14]兰芙蓉,陈文燕,王明章.儿童支气管哮喘患者血清骨桥蛋白和嗜酸细胞趋化因子表达及临床意义[J].临床肺科杂志,2017,22(5):877-879.
[15]吴丽梅,黄丽素,徐兰飞.寒喘祖帕颗粒联合沙丁胺醇雾化吸入治疗小儿支气管哮喘的疗效及对血清MPO、PMN、MCP-4的影响[J].中国生化药物杂志,2017,37(4):87-90.
[16]谢中勇,陈伟明,张伟忠,等.支气管哮喘患儿血清中酶类多肽、趋化因子水平及外周血免疫细胞含量的评估[J].海南医学院学报,2015,21(8):1103-1105.
[17]杨振明,吴丙美,寇永妹,等.舒利迭对儿童哮喘的治疗作用及对血清中MMP-2、MMP-3和MMP-9的影响[J].现代中西医结合杂志,2015,24(11):1187-1188.
[18]谢泽周.学龄期哮喘患儿治疗前后血清TIMP-1、IL-10、hs-CRP及TH的变化[J].罕少疾病杂志,2016,23(5):23-25.