共生菌对小鼠外周血及黏膜相关组织中趋化因子配体24和胰岛素样生长因子1表达的调控
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摘要
目的探讨共生菌群对小鼠外周血及黏膜相关组织(肺脏、肝脏)中趋化因子配体24(CCL24)及胰岛素样生长因子1(IGF-1)表达的调控作用。方法利用ELISA方法检测共生菌缺失小鼠(Abx鼠)及正常小鼠(WT鼠)血清中、肺脏及肝脏组织匀浆中CCL24及IGF-1的浓度,并对其表达量进行分析比较。结果小鼠缺失共生菌后,其血清中CCL24浓度上调[WT与Abx,(1. 018±0. 043)μg/L与(1. 509±0. 119)μg/L],而IGF-1浓度无显著变化[WT与Abx,(1. 240±0. 019)μg/L与(1. 250±0. 066)μg/L];其肺脏中CCL24[WT与Abx,(11. 984±0. 683) ng/g与(14. 626±0. 769) ng/g]和IGF-1[WT与Abx,(0. 998±0. 024) ng/g与(1. 290±0. 031) ng/g]表达量均显著上调;其肝脏中CCL24(WT与Abx,(11. 575±0. 609) ng/g与(8. 704±0. 458) ng/g]和IGF-1[WT与Abx,(2. 989±0. 073) ng/g与(1. 112±0. 027) ng/g]表达量均显著下调。结论共生菌对小鼠外周血、肺脏、肝脏中CCL24和IGF-1表达调控具有组织特异性。
Objective To explore the regulation of symbiotic microbiota on the expression of chemokine ligand24( CCL24) and insulin-like growth factor 1( IGF-1) in peripheral blood and mucosal associated organs( lung and liver) in mouse. Methods Enzyme-linked immunosorbent assay( ELISA) was used to test the concentrations of CCL24 and IGF-1 in the serum,lung-homogenate and liver-homogenate of wild-type( WT) mouse and antibiotics-treated( Abx)mouse,then the expression levels were statistically analyzed. Results After the commensal bacteria were depleted by the antibiotics,the serum CCL24[WT vs Abx,( 1. 018 ± 0. 043) μg/L vs( 1. 509 ± 0. 119) μg/L],but not IGF-1[WT vs Abx,( 1. 240 ± 0. 019) μg/L vs( 1. 250 ± 0. 066) μg/L],was upregulated in Abx mouse; both CCL24[WT vs Abx,( 11. 984 ± 0. 683) ng/g vs( 14. 626 ± 0. 769) ng/g] and IGF-1[WT vs Abx,( 0. 998 ± 0. 024) ng/g vs( 1. 290 ±0. 031) ng/g] were upregulated in the lung of Abx mouse; both CCL24[WT vs Abx,( 11. 575 ± 0. 609) ng/g vs( 8. 704 ± 0. 458) ng/g]and IGF-1[WT vs Abx( 2. 989 ± 0. 073) ng/g vs( 1. 112 ± 0. 027) ng/g]were downregulated in the liver of the Abx mouse. Conclusion The symbiotic microbiota can regulate the expression of CCL24 and IGF-1 in peripheral blood,lung and liver in a tissue-specific manner.
Objective To explore the regulation of symbiotic microbiota on the expression of chemokine ligand24( CCL24) and insulin-like growth factor 1( IGF-1) in peripheral blood and mucosal associated organs( lung and liver) in mouse. Methods Enzyme-linked immunosorbent assay( ELISA) was used to test the concentrations of CCL24 and IGF-1 in the serum,lung-homogenate and liver-homogenate of wild-type( WT) mouse and antibiotics-treated( Abx)mouse,then the expression levels were statistically analyzed. Results After the commensal bacteria were depleted by the antibiotics,the serum CCL24[WT vs Abx,( 1. 018 ± 0. 043) μg/L vs( 1. 509 ± 0. 119) μg/L],but not IGF-1[WT vs Abx,( 1. 240 ± 0. 019) μg/L vs( 1. 250 ± 0. 066) μg/L],was upregulated in Abx mouse; both CCL24[WT vs Abx,( 11. 984 ± 0. 683) ng/g vs( 14. 626 ± 0. 769) ng/g] and IGF-1[WT vs Abx,( 0. 998 ± 0. 024) ng/g vs( 1. 290 ±0. 031) ng/g] were upregulated in the lung of Abx mouse; both CCL24[WT vs Abx,( 11. 575 ± 0. 609) ng/g vs( 8. 704 ± 0. 458) ng/g]and IGF-1[WT vs Abx( 2. 989 ± 0. 073) ng/g vs( 1. 112 ± 0. 027) ng/g]were downregulated in the liver of the Abx mouse. Conclusion The symbiotic microbiota can regulate the expression of CCL24 and IGF-1 in peripheral blood,lung and liver in a tissue-specific manner.
引文
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[5]CHEADLE E J,RIYAD K,SUBAR D,et al.Eotaxin-2 and colorectal cancer:a potential target for immune therapy[J].Clin Cancer Res,2007,13(19):5719-5728.
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[7]CARDOSO A L,FERNANDES A,AGUILAR-PIMENTELJ A,et al.Towards frailty biomarkers:Candidates from genes and pathways regulated in aging and age-related diseases[J].Ageing Res Rev,2018,47:214-277.
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