控温超声提取-高效液相色谱法测定空气PM_(2.5)中16种多环芳烃

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英文篇名：HPLC Determinaton of 16 Polycyclic Aromatic Hydrocarbons in Atmospheric PM_(2.5) with Ultrasonic Extraction of Temperature Control 作者：史俊文 ; 朱小红 ; 汪庆庆 ; 钱晓燕 ; 刘凤芝 英文作者：SHI Junwen;ZHU Xiaohong;WANG Qingqing;QIAN Xiaoyan;LIU Fengzhi;Suzhou Industrial Park Centers for Disease Control and Prevention;Jiangsu Provincial Center for Disease Control and Prevention; 关键词：高效液相色谱法 ; 超声提取 ; 控温 ; 多环芳烃 ; 空气 ; PM2.5 英文关键词：HPLC;;ultrasonic extraction;;temperature control;;polycyclic aromatic hydrocarbon;;atmosphere;;PM2.5 中文刊名：LHJH 英文刊名：Physical Testing and Chemical Analysis(Part B:Chemical Analysis) 机构：苏州工业园区疾病防治中心;江苏省疾病预防控制中心; 出版日期：2019-02-18 出版单位：理化检验(化学分册) 年：2019 期：v.55 基金：江苏省医学创新团队(CXTDB2017012) 语种：中文; 页：LHJH201902003 页数：5 CN：02 ISSN：31-1337/TB 分类号：18-22

摘要

剪碎的滤膜经乙腈于35℃超声提取60 min,提取液以12 000r·min~(-1)转速离心10min,采用高效液相色谱法测定上清液中16种多环芳烃的含量。以Agilent Eclipse PAH-C18色谱柱为分离柱,用乙腈和水以不同比例混合的溶液为流动相进行梯度洗脱,用二极管阵列检测器和荧光检测器测定。16种多环芳烃的质量浓度均在0.02～1.00mg·L~(-1)内与其对应的峰面积呈线性关系,检出限(3S/N)为0.05～0.25ng·m~(-3)。以空白滤膜为基体进行加标回收试验,所得回收率为87.8%～103%,回收量的相对标准偏差(n=6)小于5.0%。
        The shredded filter membrane was extracted ultrasonically for 60 min with acetonitrile at 35 ℃.The extract was centrifugated for 10 min at the speed of 12 000 r·min~(-1).HPLC was applied to the determinaton of 16 polycyclic aromatic hydrocarbons in the supernatant.Agilent Eclipse PAH-C18 chromatographic column was used as separation column,and the mixture of acetonitrile and water with different mixing ratios was used as mobile phase in the gradient elution.Photodiode array detector and fluorescence detector were adopted in the determination.Linear relationships between values of peak area and mass concentration of the 16 polycyclic aromatic hydrocarbons were kept in the same range of 0.02~(-1).00 mg·L~(-1),with detection limits(3 S/N)in the range of 0.05-0.25 ng·m-3.On the base of blank filter membrane,test for recovery was made by standard addition method;values of recovery found were in the range of 87.8%~(-1)03%,with RSDs(n=6)of recovery amounts less than 5.0%.

引文

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