玉米须总黄酮抗糖基化、乙酰胆碱酯酶和α-葡萄糖甘酶抑制活性分析
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摘要
研究玉米须总黄酮抗糖基化、乙酰胆碱酯酶和α-葡萄糖甘酶抑制活性。采用内部沸腾法提取玉米须总黄酮,优化其工艺条件,并对其进行分析。在单因素实验基础上,采用Plackett-Burman法对7个相关因素进行评价,筛选出具有显著性差异的4个因素,并对其进行响应面优化。结果表明,内部沸腾法提取玉米黄酮最佳条件为解析时间16 min,解析剂浓度73%,提取时间20 min,提取温度为61℃,总黄酮提取得率为4.75±0.69%;玉米须黄酮对晚期糖基化终产物、乙酰胆碱酯酶和α-葡萄糖苷酶具有一定抑制效果,呈现浓度依赖性,IC50分别为467.90、73.07、151.04μg/mL。抗糖基化、乙酰胆碱酯酶和α-葡萄糖甘酶抑制活性与黄酮含量呈正相关(相关系数分别为0.83、0.90和0.87; P <0.05),该研究结果可为潜在的天然产物抑制剂开发提供理论依据。
To study the antiglycation activity,acetylcholine esterase and α-glucosidase inhibitory activity of total flavonoids from corn silk.Seven parameters potentially influencing the extraction yield of total flavonoids were screened through PlackettBurman design to determine the significant variables.Then,factors effecting on extraction yield were further optimized using Box-Behnken design to predict optimum extraction conditions.Satisfactory yield of total flavonoids(4.75±0.69%) was achieved using the optimal conditions.16 min of desorption time,73% of ethanol indesorption solvent,20 min of extraction time,and 61 ℃ of extraction temperature were selected and applied for effective extraction.It also exhibited the certain antiglycation,inhibitory activity against acetylcholine esterase and α-glucosidase in a concentration-dependent manner with IC50 of 467.90,73.07,and 151.04 μg/mL,respectively.Antiglycation activity,acetylcholine esterase and α-glucosidase activity inhibitory activity were positively correlated with the content of total flavonoids,whose correlation coefficients are 0.83,0.90 and 0.87 respectively,P < 0.05.It can provide a theoretical basis for the development of natural inhibitors.
To study the antiglycation activity,acetylcholine esterase and α-glucosidase inhibitory activity of total flavonoids from corn silk.Seven parameters potentially influencing the extraction yield of total flavonoids were screened through PlackettBurman design to determine the significant variables.Then,factors effecting on extraction yield were further optimized using Box-Behnken design to predict optimum extraction conditions.Satisfactory yield of total flavonoids(4.75±0.69%) was achieved using the optimal conditions.16 min of desorption time,73% of ethanol indesorption solvent,20 min of extraction time,and 61 ℃ of extraction temperature were selected and applied for effective extraction.It also exhibited the certain antiglycation,inhibitory activity against acetylcholine esterase and α-glucosidase in a concentration-dependent manner with IC50 of 467.90,73.07,and 151.04 μg/mL,respectively.Antiglycation activity,acetylcholine esterase and α-glucosidase activity inhibitory activity were positively correlated with the content of total flavonoids,whose correlation coefficients are 0.83,0.90 and 0.87 respectively,P < 0.05.It can provide a theoretical basis for the development of natural inhibitors.
引文
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2 Tian Y.Study on extraction,purification and bioactivies of flavonids in blueberry[D].Harbin:Northeast Agricultural U-niversity(东北农业大学),2012.
3 Zhuang XY.Effect of flavonoids from seed residues of Hippophae rhamnoides L.on advance glycation end products formation and AGEs-induced endothelial cell dysfunction[D].Shanghai:East China Normal University(华东师范大学),2011.
4 Feng J,Zhang FR,Yang XH,et al.Phytochemicals of three kinds of se-enriched compositae flowers and their effects on activity ofα-glucosidase[J].Mol Plant Breeding(分子植物育种),2019,17:2341-2349.
5 Hu YL,Ya N,Li X,etal.Study on the flavonoids in Blumea balsamifera DC.and their antioxidant activity as well asα-glucosidase inhibitory activity[J].Nat Prod Res Dev(天然产物研究与开发),2018,30:1898-1903.
6 Zheng YJ.Extraction,separation and pharmacological activity assay of garlic’s functional components via molecular imprinting technique[D].Qingdao:Ocean University of China(中国海洋大学),2012.
7 Li YQ,Zhou FC,Gao F,et al.Comparative evaluation of quercetin,isoquercetin and rutin as inhibitors ofα-glucosidase[J].J Agric Food Chem,2009,57:11463-11468.
8 Ebrahimzadeh MA,Pourmorad F,Hafezi S.Antioxidant activities of Iranian corn silk[J].Turk J Biol,2008,32(1):43-49.
9 Chen S,Chen H,Tian J,et al.Enzymolysis-ultrasonic assisted extraction,chemical characteristics and bioactivities of polysaccharides from corn silk[J].Carbohyd Polym,2014,101:332-341.
10 Chaiittianan R,Chayopas P,Rattanathongkom A,et al.Antiobesity potential of corn silks:relationships of phytochemicals and antioxidation,anti-pre-adipocyte proliferation,anti-adipogenesis,and lipolysis induction[J].J Funct Foods,2016,23:497-510.
11 Bai JH,Zhao Y,Guo XX,etal.Study on microwave-assisted extraction of flavonoids from com silk[J].Chin Agr Sci Bulletin(中国农学通报),2010,26(2):71-73.
12 Li FL,Xu L.Flavonoids extraction from maize silk and its function on blood sugar control[J].China Food Addit(中国食品添加剂),2009,3:121-124.
13 Fang M,Zhan CG,Gong ZY.Extraction and antioxidant activities of total flavonoids from corn silk[J].Food Sci(食品科学),2009,18:206-208.
14 Liu CW,Chen XG,Wei TY,et al.Response surface methodology for optimization of capsaicin extraction by vacuum innner ebullition[J].Food Sci(食品科学),2013,34(10):72-76.
15 Sun ZX,Hou SY,Ling HB,et al.Correlation analysis on rutin accumulation and gene expression of rutin synthetic enzymes and MYBs in the whole development stage of fagopyrum tetericum.[J].Scientia Agr Sci(中国农业科学),2017,50:3473-3481.
16 Tan L,Zheng XY,Wang JS,et al.Antioxidant activity and inhibitory activity of ethanol extract from ficus auriculata fruits againstα-Glucosidase and acetylcholine esterase[J].Food Sci(食品科学),2016,37(13):77-81.
17 Wang ZY,Zhang LH,Wang YH,et al.Optimization of ultrasonic-assisted cellulase-based extraction and anti-α-glucosidase activity of polyphenols from pomegranate fruitlets[J].Food Sci(食品科学),2017,38:214-221.
18 Zhao WZ.The evalution of bioactivity and hypoglycemic mechanism from com silk[D].Changchun:Jilin University(吉林大学),2014.