小麦开花后籽粒多组分营养物质积累动态分析
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摘要
为探究小麦开花后籽粒中多组分营养物质积累的动态规律及其相互间的关系,本研究以六倍体栽培小麦中国春和贵紫1号为材料,对开花后10、15、20、25、30、35、40d的籽粒总类黄酮、总酚、植酸、无机磷、总淀粉、氨基和可溶性蛋白质含量及单粒重进行了动态测定及单因素方差(One-Way ANOVA)、相关性网络(Correlation-based network analysis,CAN)和斯皮尔曼秩相关(Spearmanp’s Rho Correlation)等分析。结果表明,在籽粒发育过程中,氨基、可溶性蛋白质以及无机磷含量逐渐降低,淀粉含量升高后逐渐趋于稳定,总酚含量先降低后升高,总类黄酮含量及单粒重先升高后降低。2个小麦品种籽粒中各营养物质含量及单粒重的动态变化趋势相似,但各营养物质含量的高低及其差异显著性却各有不同。不同阶段的单粒重与对应阶段营养物质的含量存在不同程度的相关性,即使是同一种营养物质,在籽粒发育的不同阶段与单粒重的相关性也不尽相同。
In order to explore the dynamic law of the accumulation of multi-component nutrients in the grains of wheat after flowering and the relationship between them,we used the hexaploid-cultivated wheats(Chinese spring and Guizi no.1)as materials.The dynamics measurement of main nutritional components content such as the grain total flavonoids,total phenol,phytic acid,inorganic phosphorus,total starch,amino and soluble protein content and single grain weight at 10,15,20,25,30,35 and 40 days after flowering were measured and related data were analyzed by one-way ANOVA,Correlation-based network analysis(CAN)and Spearmanp's Rho Correlation.The results showed that during grain development,the content of amino group,soluble protein and inorganic phosphorus gradually decreased,the content of starch gradually became stable after increasing,the content of total phenol first decreased and then increased,and the content of total flavonoids and single grain weight first increased and then decreased.The dynamic change trend of each nutrient content and single grain weight in the grains of the two wheat varieties were similar,but the levels of each nutrient content and their significant differences were different.There were different degrees of correlation between single grain weight at different stages and the content of nutrients at corresponding stages.Even for the same nutrient substance at different stages of grain development,it has different correlations with single grain weight.
In order to explore the dynamic law of the accumulation of multi-component nutrients in the grains of wheat after flowering and the relationship between them,we used the hexaploid-cultivated wheats(Chinese spring and Guizi no.1)as materials.The dynamics measurement of main nutritional components content such as the grain total flavonoids,total phenol,phytic acid,inorganic phosphorus,total starch,amino and soluble protein content and single grain weight at 10,15,20,25,30,35 and 40 days after flowering were measured and related data were analyzed by one-way ANOVA,Correlation-based network analysis(CAN)and Spearmanp's Rho Correlation.The results showed that during grain development,the content of amino group,soluble protein and inorganic phosphorus gradually decreased,the content of starch gradually became stable after increasing,the content of total phenol first decreased and then increased,and the content of total flavonoids and single grain weight first increased and then decreased.The dynamic change trend of each nutrient content and single grain weight in the grains of the two wheat varieties were similar,but the levels of each nutrient content and their significant differences were different.There were different degrees of correlation between single grain weight at different stages and the content of nutrients at corresponding stages.Even for the same nutrient substance at different stages of grain development,it has different correlations with single grain weight.
引文
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[23]胡阳阳,卢红芳,刘卫星,等.灌浆期高温与干旱胁迫对小麦籽粒淀粉合成关键酶活性及淀粉积累的影响[J].作物学报,2018,44(4):591-600.
[24]韩卫娟,李加茹,李华威,等.不同(品)种柿叶总酚与总黄酮含量年动态变化研究[J].中国农业大学学报,2016,21(2):31-40.
[25]Oltmans S E,Fehr W R,Welke G A,et al.Agronomic and Seed Traits of Soybean Lines with Low Phytate Phosphorus[J].Crop Science,2005,45(2):593-598.
[2]何菁菁,PEXELS.小麦:人类较早驯化的作物[J].人与自然,2017(10):88-91.
[3]王健.小麦MADS-box基因TaAGL32的功能研究[D].山东农业大学,2017.
[4]朱婷.基于三阶段DEA模型的我国小麦主产区小麦生产效率分析[D].江南大学,2016.
[5]凡迪,薛文韬,严俊,等.四倍体小麦籽粒多组分营养物质含量的QTL定位及相关性分析[J].麦类作物学报,2014,34(12):1 611-1 618.
[6]张俊英,严俊,阮景军,等.贵州栽培大麦不同种植环境籽粒多组分营养物质质量分数分析[J].西南师范大学学报(自然科学版),2017,42(4):61-67.
[7]惠富平,过慈明.近代中国关于化肥利弊的争论[J].南京农业大学学报(社会科学版),2015,15(1):114-122,127.
[8]Rodda M S,Davidson J,Javid M,et al.Molecular Breeding for Ascochyta Blight Resistance in Lentil:Current Progress and Future Directions[J].Frontiers in Plant Science,2017,8:1 136.
[9]Xu K.The rejection of science frames in the news coverage of the golden rice experiment in Hunan,China[J].Health Risk&Society,2014,16(4):339-354.
[10]詹志杰.野生二粒小麦群体籽粒高氮基因型的筛选[D].贵州大学,2016.
[11]凡迪.小麦植酸含量的QTL定位及其相关分析[D].贵州大学,2015.
[12]Shewry P R.Wheat[J].Journal of Experimental Botany,2009,60(6):1 537-1 553.
[13]李念,阮景军,严俊,等.基因型和环境对以色列野生二棱大麦籽粒营养品质的影响[J].麦类作物学报,2017,37(12):1 564-1 570.
[14]郑许光,齐军仓,王凤,等.青稞籽粒灌浆期淀粉代谢酶活性与淀粉积累特征的关系研究[J].种子,2018,37(2):19-23,28.
[15]李解,杨荣志,程剑平,等.大麦籽粒蛋白质含量的QTL定位分析[J].种子,2013,32(9):6-9.
[16]赵丽那,阮景军,程剑平,等.燕麦籽粒多组分营养性状的相关性分析[J].江西农业大学学报,2016,38(4):623-630.
[17]韦小宝,詹志杰,严俊,等.野生二粒小麦与栽培小麦农艺性状及籽粒营养组分比较研究[J].山地农业生物学报,2017,36(1):40-44,60.
[18]李念,阮景军,严俊,等.以色列野生二棱大麦籽粒多组分营养性状的相关性分析[J].安徽农业大学学报,2016,43(6):1 017-1 023.
[19]王新坤,仲磊,杨润强,等.植物籽粒中植酸及其降解方法与产物研究进展[J].食品科学,2014,35(3):301-306.
[20]张小华,段晓亮,侯春雨,等.小麦籽粒植酸和无机磷含量的变异及其与千粒重、容重的相关性[J].中国农业大学学报,2014,19(5):8-12.
[21]石培春,李英枫,韩璐,等.不同品质类型小麦籽粒淀粉含量积累的动态差异[J].石河子大学学报(自然科学版),2012,30(4):417-421.
[22]Hurkman W J,Mccue K F,Altenbach S B,et al.Effect of temperature on expression of genes encoding enzymes for starch biosynthesis in developing wheat endosperm[J].Plant Science,2003,164(5):873-881.
[23]胡阳阳,卢红芳,刘卫星,等.灌浆期高温与干旱胁迫对小麦籽粒淀粉合成关键酶活性及淀粉积累的影响[J].作物学报,2018,44(4):591-600.
[24]韩卫娟,李加茹,李华威,等.不同(品)种柿叶总酚与总黄酮含量年动态变化研究[J].中国农业大学学报,2016,21(2):31-40.
[25]Oltmans S E,Fehr W R,Welke G A,et al.Agronomic and Seed Traits of Soybean Lines with Low Phytate Phosphorus[J].Crop Science,2005,45(2):593-598.