典型底栖生物泥鳅的急性毒性物种敏感性评价
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摘要
泥鳅(Misgumus anguillicadatus)是重要的本土底栖鱼类。通过搜集、筛选氨氮、重金属(Ni~(2+)、Cu~(2+))、农药(敌敌畏、硫丹)和苯系物(硝基苯)对泥鳅的急性毒性数据,利用物种敏感性分布曲线评价泥鳅对各污染物的敏感性。结果表明,氨氮、硝基苯、敌敌畏、硫丹、Cu~(2+)对泥鳅为剧毒级污染物[ρ(LC_(50))<1 mg·L~(-1)],其中泥鳅对敌敌畏敏感度最高,物种累积概率为5.88%。泥鳅对Ni~(2+)的耐受性较强,对该污染物的胁迫不敏感。与模式鱼类相比,泥鳅对敌敌畏、硫丹、Cu~(2+)和硝基苯的敏感性远高于斑马鱼(Danio rerio),体现出泥鳅具有作为上述污染物监测指示物种的潜力。综上,泥鳅可作为研究农药、Cu~(2+)和硝基苯水质基准的受试生物。
Misgumus anguillicadatus is a representative ground fish endemic to China. The data of acute ecotoxicity of ammonia, heavy metals(Ni~(2+) and Cu~(2+)), pesticides(dichlorvos and endosulfan), and nitrobenzene to Misgumus anguillicadatus were screened and collected. The species sensitivity distribution method was used to evaluate the sensitivity of Misgumus anguillicadatus to chemicals above. According to the toxicity grade, ammonia, dichlorvos, endosulfan, and Cu~(2+) were extremely toxic to Misgumus anguillicadatus while nitrobenzene was highly toxic [ρ(LC_(50))<1 mg·L~(-1)]. Misgumus anguillicadatus was especially susceptible to dichlorvos with the cumulative probability of 5.88% and was relatively tolerant to Ni~(2+). Compared to the model fish, Danio rerio, Misgumus anguillicadatus showed a higher sensitivity to dichlorvos, endosulfan, Cu~(2+), and nitrobenzene, indicating its potency of being a bio-indicator for those substances. Misgumus anguillicadatus can be used as the experimental subject for water quality criteria studies of pesticides, Cu~(2+), and nitrobenzene.
Misgumus anguillicadatus is a representative ground fish endemic to China. The data of acute ecotoxicity of ammonia, heavy metals(Ni~(2+) and Cu~(2+)), pesticides(dichlorvos and endosulfan), and nitrobenzene to Misgumus anguillicadatus were screened and collected. The species sensitivity distribution method was used to evaluate the sensitivity of Misgumus anguillicadatus to chemicals above. According to the toxicity grade, ammonia, dichlorvos, endosulfan, and Cu~(2+) were extremely toxic to Misgumus anguillicadatus while nitrobenzene was highly toxic [ρ(LC_(50))<1 mg·L~(-1)]. Misgumus anguillicadatus was especially susceptible to dichlorvos with the cumulative probability of 5.88% and was relatively tolerant to Ni~(2+). Compared to the model fish, Danio rerio, Misgumus anguillicadatus showed a higher sensitivity to dichlorvos, endosulfan, Cu~(2+), and nitrobenzene, indicating its potency of being a bio-indicator for those substances. Misgumus anguillicadatus can be used as the experimental subject for water quality criteria studies of pesticides, Cu~(2+), and nitrobenzene.
引文
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[10] 郝小凤,刘洋,凌去非.氨氮对泥鳅的急性毒性及对其肝、鳃组织超微结构的影响[J].水生态学杂志,2012(5):101-107.[HAO Xiao-feng,LIU Yang,LING Qu-fei.Acute Toxicity Test of Ammonia Nitrogen and Effects of Ammonia-N Stress on the Ultrastructure of Gill and Liver of Misgurnus anguillicaudatus[J].Journal of Hydroecology,2012(5):101-107.]
[11] MONDAL D,BARAT S,MUKHOPADHYAY M K.Toxicity of Neem Pesticides on A Fresh Water Loach,Lepidocephalichthys guntea (Hamilton Buchanan) of Darjeeling District in West Bengal[J].Journal of Environmental Biology,2007,28(1):119-122.
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[16] 卢辰华.两种重金属钴和镍对两种泥鳅的毒性效应[D].新乡:河南师范大学,2016.[LU Chen-hua.Two Heavy Metal Cobalt and Nickel′s Toxicity Effects on Two Different Loaches [J].Xinxiang:Henan Normal University,2016.]
[17] 刘民.亚硒酸盐对泥鳅毒性作用的研究[D].济南:山东师范大学,2007.[LIU Min.A Research on Toxicity Effects of Selenite on Loach [D].Jinan:Shandong Normal University,2007.]
[18] 余麟.辽河源头区水质变化趋势与污染构成研究[D].长春:吉林大学,2013.[YU Lin.A Research on Water Quality Change and Pollution in the Headwaters of Liaohe River [D].Changchun:Jilin University,2013.]
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[21] 赵艳民,秦延文,郑丙辉,等.突发性水污染事故应急健康风险评价[J].中国环境科学,2014(5):1328-1335.[ZHAO Yan-min,QIN Yan-wen,ZHENG Bing-hui,et al.Emergency Health Risk Assessment of Water Pollution Accident[J].China Environmental Science,2014(5):1328-1335.]
[22] 国家环境保护局.环境监测技术规范第四册:生物监测(水环境)部分[M].北京:中国环境科学出版社,1986:43.
[23] WU J,LIU Z,YAN Z,et al.Derivation of Water Quality Criteria of Phenanthrene Using interspecies Correlation Estimation Models for Aquatic Life in China[J].Environmental Science and Pollution Research,2015,22(12):9457-9463.
[24] 刘亚莉,谢玉为,张效伟,等.应用物种敏感性分布评价敌敌畏对淡水生物的生态风险[J].生态毒理学报,2016,11(2):531-538.[LIU Ya-li,XIE Yu-wei,ZHANG Xiao-wei,et al.Assessing Ecological Risks of Dichlorvos to Freshwater Organisms By Species Sensitivity Distribution[J].Asian Journal of Ecotoxicology,2016,11(2):531-538.]
[25] 徐瑞祥,陈亚华.应用物种敏感性分布评估有机磷农药对淡水生物的急性生态风险[J].湖泊科学,2012,24(6):811-821.[XU Rui-xiang,CHEN Ya-hua.Assessing Acute Ecological Risks of Organophosphorus Pesticides to Freshwater Organisms by Species Sensitivity Distributions[J].Journal of Lake Sciences.2012,24(6):811-821.]
[26] WU J Y,YAN Z G,LIU Z T,et al.Development of Water Quality Criteria for Phenanthrene and Comparison of the Sensitivity Between Native and Non-Native Species[J].Environmental Pollution,2015,196:141-146.
[27] 王振,金小伟,王子健.铜对水生生物的毒性:类群特异性敏感度分析[J].生态毒理学报,2014(4):640-646.[WANG Zhen,JIN Xiao-wei,WANG Zi-jian.Taxon-Specific Sensitvity Differences of Copper to Aquatic Organisms[J].Asian Journal of Ecotoxicology,2014(4):640-646.]
[28] 吴丰昌,冯承莲,张瑞卿,等.我国典型污染物水质基准研究[J].中国科学:地球科学,2012,42(5):665-672.
[29] 石小荣,李梅,崔益斌,等.以太湖流域为例探讨我国淡水生物氨氮基准[J].环境科学学报,2012,32(6):1406-1414.[SHI Xiao-rong,LI Mei,CUI Yi-bin,et al.Development of Aquatic Water Quality Criteria for Ammonia in Freshwater Ecosystem of China Based on Lake Tai Basin[J].Acta Scientiae Circumstantiae,2012,32(6):1406-1414.]
[30] 闫振广,孟伟,刘征涛,等.我国淡水生物氨氮基准研究[J].环境科学,2011,32(6):1564-1570.[YAN Zhen-guang,MENG Wei,LIU Zheng-tao,et al.Development of Freshwater Aquatic Life Criteria for Ammonia in China[J].Environmental Science,2011,32(6):1564-1570.]
[31] 刘炎.环境因子对氨氮毒性影响及氨氮水生生物基准校验[D].南京:南京大学,2014.[LIU Yan.The Validation of the Environmental Factors′ Influences on the Toxicity and Aquatic Criteria of Ammonia [D].Nanjing:Nanjing University,2014.]
[32] ZHAO J,ZHU X,XU T,et al.Structure-Dependent Activities of Polybrominated Diphenyl Ethers and Hydroxylated Metabolites on Zebrafish Retinoic Acid Receptor[J].Environmental Science and Pollution Research International,2015,22(3):1723-1730.
[33] 周绍辉,杜娟娟,孙中训,等.基于斑马鱼行为变化的苯酚监测研究[J].安徽农业科学,2017,45(1):83-85,93.[ZHOU Shao-hui,DU Juan-juan,SUN Zhong-xun,et al.Research of Phenol Monitoring Based on the Behavior Changes of Brachydanio rerio[J].Journal of Anhui Agricultural Science,2017,45(1):83-85,93.]
[34] CARRIGER J F,HOANG T C,RAND G M,et al.Acute Toxicity and Effects Analysis of Endosulfan Sulfate to Freshwater Fish Species[J].Archives of Environmental Contamination and Toxicology,2011,60(2):281-289.
[2] ZHANG Y,ZANG W,QIN L,et al.Water Quality Criteria for Copper Based on the BLM Approach in the Freshwater in China[J].PLoS one,2017,12(2):e0170105.
[3] 王晓南,刘征涛,闫振广,等.麦穗鱼物种敏感性评价[J].环境科学,2013,34(6):2329-2334.[WANG Xiao-nan,LIU Zheng-tao,YAN Zhen-guang,et al.Species Sensitivity Evaluation of Pseudorasbora parva[J].Environmental Science,2013,34(6):2329-2334.]
[4] 王伟莉,闫振广,何丽,等.五种底栖动物对优控污染物的敏感性评价[J].中国环境科学,2013(10):1856-1862.[WANG Wei-li,YAN Zhen-guang,HE Li,et al.Sensitivity Evaluation of Five Zoobenthos to Priority Pollutants[J].China Environmental Science,2013(10):1856-1862.]
[5] 王晓南,郑欣,闫振广,等.水质基准鱼类受试生物筛选[J].环境科学研究,2014(4):341-348.[WANG Xiao-nan,ZHENG Xin,YAN Zhen-guang,et al.Screening of Native Fishes for Deriving Aquatic Life Criteria[J].Research of Environmental Sciences,2014(4):341-348.]
[6] 陈玉明,卢少勇,朱旭,等.泥鳅在污染物毒性评价中的应用[J].生态毒理学报,2013(4):447-455.[CHEN Yu-ming,LU Shao-yong,ZHU Xu,et al.Review on Application of Loach to Assessing Toicity of Pollutants[J].Asian Journal of Ecotoxicology,2013(4):447-455.]
[7] 杨启超,万全,赵俊峰,等.4种常用鱼药对泥鳅的急性毒性试验[J].水利渔业,2006(2):93-95.
[8] 南平,肖珍,陈建军,等.敌敌畏农药的极性暴露对泥鳅的遗传毒性及生理影响[J].河南师范大学学报(自然科学版),2011(5):137-140.[NAN Ping,XIAO Zhen,CHEN Jian-jun,et al.Genetics Toxicity and Physiology Toxicity of Dichlorovos to Misgurnus anguillicaudatus[J].Journal of Henan Normal University (Natural Science Edition),2011(5):137-140.
[9] 王哲娟.苯、苯酚、硝基苯对泥鳅的急性毒性及抗氧化酶活性影响的研究[D].哈尔滨:哈尔滨师范大学,2010.[WANG Zhe-juan.Effect of Benzene,Phenol and Nitrobenzene on Misgurnus bipartitus Acute Toxicity and Antioxidant Enzymes Activity[D].Harbin:Harbin Normal University,2010.]
[10] 郝小凤,刘洋,凌去非.氨氮对泥鳅的急性毒性及对其肝、鳃组织超微结构的影响[J].水生态学杂志,2012(5):101-107.[HAO Xiao-feng,LIU Yang,LING Qu-fei.Acute Toxicity Test of Ammonia Nitrogen and Effects of Ammonia-N Stress on the Ultrastructure of Gill and Liver of Misgurnus anguillicaudatus[J].Journal of Hydroecology,2012(5):101-107.]
[11] MONDAL D,BARAT S,MUKHOPADHYAY M K.Toxicity of Neem Pesticides on A Fresh Water Loach,Lepidocephalichthys guntea (Hamilton Buchanan) of Darjeeling District in West Bengal[J].Journal of Environmental Biology,2007,28(1):119-122.
[12] 高晓莉,齐凤生,罗胡英,等.铜、汞、铬对泥鳅的急性毒性和联合毒性实验[J].水利渔业,2003(2):63-64.
[13] USEPA.Aquatic Life Ambient Water Quality Criteria for Ammonia-Freshwater[R].Washington DC,USA:EPA,2013.
[14] EMERSON K,RUSSO R C,LUND R E,et al.Aqueous Ammonia Equilibrium Calculations:Effect of pH and Temperature[J].Journal of the Fisheries Research Board of Canada,1975,32(12):2379-2383.
[15] NAN P,YAN S,LI L,et al.Toxicity Effect of Dichlorvos on Loach (Misgurnus anguillicaudatus) Assessed by Micronucleus Test,Hepatase Activity Analysis and Comet Assay[J].Toxicology Industry Health,2015,31(6):566-575.
[16] 卢辰华.两种重金属钴和镍对两种泥鳅的毒性效应[D].新乡:河南师范大学,2016.[LU Chen-hua.Two Heavy Metal Cobalt and Nickel′s Toxicity Effects on Two Different Loaches [J].Xinxiang:Henan Normal University,2016.]
[17] 刘民.亚硒酸盐对泥鳅毒性作用的研究[D].济南:山东师范大学,2007.[LIU Min.A Research on Toxicity Effects of Selenite on Loach [D].Jinan:Shandong Normal University,2007.]
[18] 余麟.辽河源头区水质变化趋势与污染构成研究[D].长春:吉林大学,2013.[YU Lin.A Research on Water Quality Change and Pollution in the Headwaters of Liaohe River [D].Changchun:Jilin University,2013.]
[19] 张旭.我国淡水中铜的水质基准及生态风险评估研究[D].南昌:南昌大学,2016.[ZHANG Xu.Water Quality Criteria and Ecological Risk Assessment of Copper in Freshwater in China [D].Nanchang:Nanchang University,2016.]
[20] 王华夫,李微微.中国农药污染情况及防治措施的建议[C]//2004年全国农业面源污染与综合防治学术研讨会论文集.北京:中国农学会,2004.
[21] 赵艳民,秦延文,郑丙辉,等.突发性水污染事故应急健康风险评价[J].中国环境科学,2014(5):1328-1335.[ZHAO Yan-min,QIN Yan-wen,ZHENG Bing-hui,et al.Emergency Health Risk Assessment of Water Pollution Accident[J].China Environmental Science,2014(5):1328-1335.]
[22] 国家环境保护局.环境监测技术规范第四册:生物监测(水环境)部分[M].北京:中国环境科学出版社,1986:43.
[23] WU J,LIU Z,YAN Z,et al.Derivation of Water Quality Criteria of Phenanthrene Using interspecies Correlation Estimation Models for Aquatic Life in China[J].Environmental Science and Pollution Research,2015,22(12):9457-9463.
[24] 刘亚莉,谢玉为,张效伟,等.应用物种敏感性分布评价敌敌畏对淡水生物的生态风险[J].生态毒理学报,2016,11(2):531-538.[LIU Ya-li,XIE Yu-wei,ZHANG Xiao-wei,et al.Assessing Ecological Risks of Dichlorvos to Freshwater Organisms By Species Sensitivity Distribution[J].Asian Journal of Ecotoxicology,2016,11(2):531-538.]
[25] 徐瑞祥,陈亚华.应用物种敏感性分布评估有机磷农药对淡水生物的急性生态风险[J].湖泊科学,2012,24(6):811-821.[XU Rui-xiang,CHEN Ya-hua.Assessing Acute Ecological Risks of Organophosphorus Pesticides to Freshwater Organisms by Species Sensitivity Distributions[J].Journal of Lake Sciences.2012,24(6):811-821.]
[26] WU J Y,YAN Z G,LIU Z T,et al.Development of Water Quality Criteria for Phenanthrene and Comparison of the Sensitivity Between Native and Non-Native Species[J].Environmental Pollution,2015,196:141-146.
[27] 王振,金小伟,王子健.铜对水生生物的毒性:类群特异性敏感度分析[J].生态毒理学报,2014(4):640-646.[WANG Zhen,JIN Xiao-wei,WANG Zi-jian.Taxon-Specific Sensitvity Differences of Copper to Aquatic Organisms[J].Asian Journal of Ecotoxicology,2014(4):640-646.]
[28] 吴丰昌,冯承莲,张瑞卿,等.我国典型污染物水质基准研究[J].中国科学:地球科学,2012,42(5):665-672.
[29] 石小荣,李梅,崔益斌,等.以太湖流域为例探讨我国淡水生物氨氮基准[J].环境科学学报,2012,32(6):1406-1414.[SHI Xiao-rong,LI Mei,CUI Yi-bin,et al.Development of Aquatic Water Quality Criteria for Ammonia in Freshwater Ecosystem of China Based on Lake Tai Basin[J].Acta Scientiae Circumstantiae,2012,32(6):1406-1414.]
[30] 闫振广,孟伟,刘征涛,等.我国淡水生物氨氮基准研究[J].环境科学,2011,32(6):1564-1570.[YAN Zhen-guang,MENG Wei,LIU Zheng-tao,et al.Development of Freshwater Aquatic Life Criteria for Ammonia in China[J].Environmental Science,2011,32(6):1564-1570.]
[31] 刘炎.环境因子对氨氮毒性影响及氨氮水生生物基准校验[D].南京:南京大学,2014.[LIU Yan.The Validation of the Environmental Factors′ Influences on the Toxicity and Aquatic Criteria of Ammonia [D].Nanjing:Nanjing University,2014.]
[32] ZHAO J,ZHU X,XU T,et al.Structure-Dependent Activities of Polybrominated Diphenyl Ethers and Hydroxylated Metabolites on Zebrafish Retinoic Acid Receptor[J].Environmental Science and Pollution Research International,2015,22(3):1723-1730.
[33] 周绍辉,杜娟娟,孙中训,等.基于斑马鱼行为变化的苯酚监测研究[J].安徽农业科学,2017,45(1):83-85,93.[ZHOU Shao-hui,DU Juan-juan,SUN Zhong-xun,et al.Research of Phenol Monitoring Based on the Behavior Changes of Brachydanio rerio[J].Journal of Anhui Agricultural Science,2017,45(1):83-85,93.]
[34] CARRIGER J F,HOANG T C,RAND G M,et al.Acute Toxicity and Effects Analysis of Endosulfan Sulfate to Freshwater Fish Species[J].Archives of Environmental Contamination and Toxicology,2011,60(2):281-289.
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