5种植物精油对赤拟谷盗的驱避活性
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摘要
为筛选新型环保植物源仓储害虫驱避剂,采用水蒸气蒸馏法提取椒蒿、香青兰、窃衣、驱虫斑鸠菊和啤酒花的植物精油,滤纸药膜法测定5种精油对赤拟谷盗成虫的驱避活性。结果表明:2 h后,在含量为16 nL/cm~2时,香青兰、窃衣、驱虫斑鸠菊、椒蒿精油对赤拟谷盗的驱避活性与阳性对照避蚊胺相当,驱避活性等级均为Ⅴ级,驱虫斑鸠菊的驱避等级稍弱于避蚊胺,驱避等级为Ⅳ级;随着浓度的降低,各精油的驱避活性随之较低,但在含量为1.6、0.16 nL/cm~2时,5种植物精油的驱避活性明显高于阳性对照(驱避率为0%);在含量为0.016 nL/cm~2时,除香青兰精油具有驱避活性(驱避率为37%),驱避等级为Ⅱ级外,其他几种植物精油的驱避活性都为Ⅰ级,甚至无驱避活性;在含量为0.001 6 nL/cm~2时,香青兰精油依然具有Ⅱ级的驱避活性,其他植物几乎均无驱避活性,有些还呈现吸引作用;4 h后,各精油的驱避活性均有所下降,但总体趋势与2 h无明显差异。研究结果表明香青兰精油对赤拟谷盗具有较强的驱避活性,值得进一步研究,该研究结果可为仓储害虫的无公害防治提供参考。
To screen environmental protection plant source storage insect repellent, and, water vapor distillation was used to extract essential oils from artemisia capsicum, vetiver, clothing theft, insect-repellent turtlewort and hops, plus, the repellent activity of 5 essential oils against adult larva ruminata was determined by filter paper membrane method. At 2 h after exposure, the result showed that at the highest concentration(16 nL/cm~2), four essential oils(A. dracunculus, D. moldavica, T. scabra, H. lupulus) exhibited the same level of repellency(class V) against the beetles compared with the positive control(DEET), but V. anthelmintica(class IV) exhibited weak repellency than DEET. At other concentrations(1.6 nL/cm~2, 0.16 nL/cm~2), all of five essential oils exhibited stronger repellency against the red flour beetle than DEET. Moreover, at the lowest concentration(0.001 6 nL/cm~2), the essential oil of D. moldavica still exhibited 32% repellency against the red flour beetles, and other essential oils had no repellency, some of them had attractive. At 4 h after exposure, repellency of five essential oils showed slightly lower than dates at 2 h after exposure, but no significant difference overall. This finding indicated that the essential oil of D. moldavica showed potential for development as a natural repellent for stored products, worth further study,and the results of this study could provide a theoretical basis for the pollution-free control of storage pests.
To screen environmental protection plant source storage insect repellent, and, water vapor distillation was used to extract essential oils from artemisia capsicum, vetiver, clothing theft, insect-repellent turtlewort and hops, plus, the repellent activity of 5 essential oils against adult larva ruminata was determined by filter paper membrane method. At 2 h after exposure, the result showed that at the highest concentration(16 nL/cm~2), four essential oils(A. dracunculus, D. moldavica, T. scabra, H. lupulus) exhibited the same level of repellency(class V) against the beetles compared with the positive control(DEET), but V. anthelmintica(class IV) exhibited weak repellency than DEET. At other concentrations(1.6 nL/cm~2, 0.16 nL/cm~2), all of five essential oils exhibited stronger repellency against the red flour beetle than DEET. Moreover, at the lowest concentration(0.001 6 nL/cm~2), the essential oil of D. moldavica still exhibited 32% repellency against the red flour beetles, and other essential oils had no repellency, some of them had attractive. At 4 h after exposure, repellency of five essential oils showed slightly lower than dates at 2 h after exposure, but no significant difference overall. This finding indicated that the essential oil of D. moldavica showed potential for development as a natural repellent for stored products, worth further study,and the results of this study could provide a theoretical basis for the pollution-free control of storage pests.
引文
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[4]LIU C H, MISHRA A K, TAN R X, et al. Repellent and insecticidal activities of essential oils from Artemisia princeps and Cinnamomum camphora and their effect on seed germination of wheat and broad bean[J]. Bioresource Technology,2006, 97:1969-1973
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[8]陆驰宇,史家浩,李绍勤.13种植物精油和茴香脑对赤拟谷盗成虫熏蒸活性研究[J].中国粮油学报,2014, 29(2):72-75LU C Y, SHI J H, LI S Q. Fumigation activities of 13 kinds of essential oils and anethole against the adult of Tribolium Castaneum(Herbst)[J]. Journal of the Chinese Cereals and Oils Association, 2014, 29(2):72-75
[9]赵雪娜,王建清.花椒精油抑制赤拟谷盗虫卵孵化作用的研究[J].中国粮油学报,2013, 28(1):82-87ZHAO X N, WANG J Q. Study on the incubation inhibition of pepper essential oils on Tribolium eggs[J]. Journal of the Chinese Cereals and Oils Association, 2013, 28(1):82-87
[10]吴彦,张文娟,李志华,等.侧柏叶对烟草仓储害虫烟草甲和赤拟谷盗的毒杀作用[J].烟草科技,2015, 48(10):31-56WU Y, ZHANG W J, LI Z H, et al. Toxic activities of Platycladus orientalis against Lasioderma serricorne and Tribolium castaneum in stored tobacco[J]. Tobacco Science and Technology, 2015,48(10):31-56
[11]杨杉,乔利利,蔡万伦,等.几种植物精油对赤拟谷盗成虫的熏蒸作用[J].中国粮油学报,2009, 24(4):122-126YANG S, QIAO L L, CAI W L, et al. Fumigant effect of several species of plant essential oil on adults of Triboliumcastaneum(Herbst)(Coleoptera:Tenebrionidae)[J].Journal of the Chinese Cereals and Oils Association, 2009,24(4):122-126
[12]徐小青,吴雨,范超敏,等.2种臭黄荆叶主要挥发性成分及驱虫作用研究[J].中国粮油学报,2013, 28(10):74-79XU X Q, WU Y, FAN C M, et al. Main volatile components comparative analysis of two kinds of premna and the controlling activities against Tribolium Castaneum[J]. Journal of the Chinese Cereals and Oils Association, 2013, 28(10):74-79
[13]彭贝,王振华,靳勇,等.辣根素对3种仓储害虫的熏蒸作用[J].中国粮油学报,2011, 26(6):82-85PENG B, WANG Z H, JIN Y, et al. Fumigation activity of AITC against three stored-grain insects[J]. Journal of the Chinese Cereals and Oils Association,2011, 26(6):82-85
[14]肖洪美,屠康.香茅精油对两种主要储粮害虫的控制作用[J].粮食储藏,2008(3):8-11XIAO H M, TU K.Effects of Cymbopogon Winterianus oil on two stored grain insect adults[J]. Grain Storage, 2008(3):8-11
[15]聂霄艳,邓永学,王进军,等.花椒精油和麻素对赤拟谷盗成虫的控制作用[J].中国粮油学报,2008(4):185-188NIE X Y, DENG Y X, WANG J J, et al. Controlling activites of prickly ash essential oils and spicy elements against Tribolium castaneum adults[J]. Journal of the Chinese Cereals and Oils Association,2008(4):185-188
[16]冯缨,潘伯荣.新疆特有种植物区系及生态学研究[J].云南植物研究,2004, 26(2):183-188FENG Y, PAN B R. Study on floristic and ecology of species endemic to Xinjiang[J]. Acta Botanica Yunnanica,2004,26(2):183-188
[17]ZHANG J S, ZHAO N N, LIU Q Z,et al. Repellent constituents of essential oil of Cymbopogon distans aerial parts against two stored-product insects[J]. Journal of Agricultural and Food Chemistry, 2011, 59(18):9910-9915
[18]TALUKDER F A, HOWSE P E. Evaluation of Aphanamixis polystachya as a source of repellents, antifeedants, toxicants and protectants in storage against Tribolium castaneum(Herbst)[J]. Journal of Stored Products Research, 1995,31(1):55-61
[19]FERRERO A A, CHOPA C S, GONZALEZ J 0,et al. Repellence and toxicity of Schinus molle extracts on Blattella germanica[J]. Fitoterapia,2007, 78:311-314
[20]LIANG Y, LI J L, XU S, et al. Evaluation of repellency of some Chinese medicinal herbs essential oils against Liposcelis bostrychophila(Psocoptera:Liposcelidae)and Tribolium castaneum(Coleoptera:Tenebrionidae)[J]. Journal of Economic Entomology, 2013, 106(1):513-519
[21]刘静,陈丽,张春红,等.3种植物精油对赤拟谷盗的控制作用[J].粮油加工,2007(5):94-97LIU J, CHEN L,ZHANG C H, et al. Bioactivities of threeessential oilsagainst Triboliumcastaneum[J]. Cereals and Oils Processing, 2007(5):94-97
[22]吕建华,王新民,白旭光,等.4种植物精油对赤拟谷盗的控制作用研究[J].河南农业科学,2006(9):68-71LYU J H,WANG X M, BAI X G, et al. Studies on the repellent activity of plant essential oils against Tribolium castaneum(Herbst)[J]. Journal of Henan Agricultural Sciences, 2006(9):68-71.
[2]董秋晨,王晓曦,刘亚楠.赤拟谷盗对小麦粉品质的影响[J].粮食与饲料工业,2012(8):9-14DONG Q C, WANG X X, LIU Y N. Research on quality properties of flour infected by red flour beetle(Tribolium canstaneum)[J]. Cereal and Feed Industry,2012(8):9-14
[3]LEE B H, CHOI W S, LEE S E, et al. Fumigant toxicity of essential oils and their constituent compounds towards the riceweevil, Sitophilus oryzae(L.)[J]. Crop Protection, 2001,20:317-320
[4]LIU C H, MISHRA A K, TAN R X, et al. Repellent and insecticidal activities of essential oils from Artemisia princeps and Cinnamomum camphora and their effect on seed germination of wheat and broad bean[J]. Bioresource Technology,2006, 97:1969-1973
[5]CHU S S, LIU S L,JIANG G H, et al. Composition and toxicity of essential oil of Illicium simonsii Maxim(Illiciaceae)fruit against the maize weevils[J]. Records of Natural Products, 2010, 4(4):205-210
[6]侯华民,张兴.植物精油对三种鳞翅目害虫的杀虫活性[J].植物保护学报,2002, 29(3):223-228HOU H M, ZHANG X. Effects of essential oil of plants on three Lepidopterous insects antifeeding and growth inhibition[J]. Acta Phytophylacica Sinica, 2002, 29(3):223-228
[7]CHU S S, LIU S L, LIU Q Z, et al. Composition and toxicity of Chinese Dracocephalum moldavica(Labiatae)essential oil against two grain storage insects[J]. Journal of Medicinal Plants Research, 2011, 5(18):4621-4626
[8]陆驰宇,史家浩,李绍勤.13种植物精油和茴香脑对赤拟谷盗成虫熏蒸活性研究[J].中国粮油学报,2014, 29(2):72-75LU C Y, SHI J H, LI S Q. Fumigation activities of 13 kinds of essential oils and anethole against the adult of Tribolium Castaneum(Herbst)[J]. Journal of the Chinese Cereals and Oils Association, 2014, 29(2):72-75
[9]赵雪娜,王建清.花椒精油抑制赤拟谷盗虫卵孵化作用的研究[J].中国粮油学报,2013, 28(1):82-87ZHAO X N, WANG J Q. Study on the incubation inhibition of pepper essential oils on Tribolium eggs[J]. Journal of the Chinese Cereals and Oils Association, 2013, 28(1):82-87
[10]吴彦,张文娟,李志华,等.侧柏叶对烟草仓储害虫烟草甲和赤拟谷盗的毒杀作用[J].烟草科技,2015, 48(10):31-56WU Y, ZHANG W J, LI Z H, et al. Toxic activities of Platycladus orientalis against Lasioderma serricorne and Tribolium castaneum in stored tobacco[J]. Tobacco Science and Technology, 2015,48(10):31-56
[11]杨杉,乔利利,蔡万伦,等.几种植物精油对赤拟谷盗成虫的熏蒸作用[J].中国粮油学报,2009, 24(4):122-126YANG S, QIAO L L, CAI W L, et al. Fumigant effect of several species of plant essential oil on adults of Triboliumcastaneum(Herbst)(Coleoptera:Tenebrionidae)[J].Journal of the Chinese Cereals and Oils Association, 2009,24(4):122-126
[12]徐小青,吴雨,范超敏,等.2种臭黄荆叶主要挥发性成分及驱虫作用研究[J].中国粮油学报,2013, 28(10):74-79XU X Q, WU Y, FAN C M, et al. Main volatile components comparative analysis of two kinds of premna and the controlling activities against Tribolium Castaneum[J]. Journal of the Chinese Cereals and Oils Association, 2013, 28(10):74-79
[13]彭贝,王振华,靳勇,等.辣根素对3种仓储害虫的熏蒸作用[J].中国粮油学报,2011, 26(6):82-85PENG B, WANG Z H, JIN Y, et al. Fumigation activity of AITC against three stored-grain insects[J]. Journal of the Chinese Cereals and Oils Association,2011, 26(6):82-85
[14]肖洪美,屠康.香茅精油对两种主要储粮害虫的控制作用[J].粮食储藏,2008(3):8-11XIAO H M, TU K.Effects of Cymbopogon Winterianus oil on two stored grain insect adults[J]. Grain Storage, 2008(3):8-11
[15]聂霄艳,邓永学,王进军,等.花椒精油和麻素对赤拟谷盗成虫的控制作用[J].中国粮油学报,2008(4):185-188NIE X Y, DENG Y X, WANG J J, et al. Controlling activites of prickly ash essential oils and spicy elements against Tribolium castaneum adults[J]. Journal of the Chinese Cereals and Oils Association,2008(4):185-188
[16]冯缨,潘伯荣.新疆特有种植物区系及生态学研究[J].云南植物研究,2004, 26(2):183-188FENG Y, PAN B R. Study on floristic and ecology of species endemic to Xinjiang[J]. Acta Botanica Yunnanica,2004,26(2):183-188
[17]ZHANG J S, ZHAO N N, LIU Q Z,et al. Repellent constituents of essential oil of Cymbopogon distans aerial parts against two stored-product insects[J]. Journal of Agricultural and Food Chemistry, 2011, 59(18):9910-9915
[18]TALUKDER F A, HOWSE P E. Evaluation of Aphanamixis polystachya as a source of repellents, antifeedants, toxicants and protectants in storage against Tribolium castaneum(Herbst)[J]. Journal of Stored Products Research, 1995,31(1):55-61
[19]FERRERO A A, CHOPA C S, GONZALEZ J 0,et al. Repellence and toxicity of Schinus molle extracts on Blattella germanica[J]. Fitoterapia,2007, 78:311-314
[20]LIANG Y, LI J L, XU S, et al. Evaluation of repellency of some Chinese medicinal herbs essential oils against Liposcelis bostrychophila(Psocoptera:Liposcelidae)and Tribolium castaneum(Coleoptera:Tenebrionidae)[J]. Journal of Economic Entomology, 2013, 106(1):513-519
[21]刘静,陈丽,张春红,等.3种植物精油对赤拟谷盗的控制作用[J].粮油加工,2007(5):94-97LIU J, CHEN L,ZHANG C H, et al. Bioactivities of threeessential oilsagainst Triboliumcastaneum[J]. Cereals and Oils Processing, 2007(5):94-97
[22]吕建华,王新民,白旭光,等.4种植物精油对赤拟谷盗的控制作用研究[J].河南农业科学,2006(9):68-71LYU J H,WANG X M, BAI X G, et al. Studies on the repellent activity of plant essential oils against Tribolium castaneum(Herbst)[J]. Journal of Henan Agricultural Sciences, 2006(9):68-71.
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