乙醇作用时间对大鼠子宫内膜损伤的影响
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摘要
目的探讨95%乙醇不同作用时间对大鼠子宫内膜损伤程度的影响。方法在大鼠宫腔内给予95%乙醇,作用时间分别为15s、30s、60s和120s;在处理后7d,观察大鼠子宫的大体形态,并采用组织形态学的方法观察大鼠子宫内膜修复情况以及子宫内膜的厚度和腺体数量分析。结果 95%乙醇作用15s组子宫腺体数量明显减少,出现纤维化;作用30s组子宫出现点状淤血,外观较光滑,宫腔狭小,纤维化面积增大;作用60s和120s组子宫管表面出现皱痕,有明显淤血,并且子宫内膜明显变薄。结论 95%乙醇处理时间15~30s对子宫内膜的损伤可能更贴近临床刮宫的损伤程度。
Objective:To explore the effect of different treatment time of 95% ethanol in rat uterine cavity on the endometrial injury.Methods:In this study,95% ethanol was injected into the rat uterine cavity for 15 s,30 s,60 sand 120 s,respectively.Seven days after the treatment,the rat uteri were collected,and the degree of endometrial repair,endometrial thickness and the number of gland were observed by morphological analysis.Results:It was shown that in the treatment group with 95% ethanol for 15 s,the number of gland in the endometrium was obviously reduced and fibrosis appeared in endometrium;In the treatment group with 95% ethanol for 30 s,the uterus showed the punctate congestions,the uterus surface was smooth,and the uterine cavity became smaller,with the area of fibrosis increased in the endometrium;Moreover,in the treatment groups with 95% ethanol for 60 sand 120 s,the uterus surface showed creases and obvious congestions,and the endometrium became much thinner.Conclusions:These results showed the status of endometrial injury in rat caused by 95% ethanol for 15～30 s,was similar to the morphological changes in human endometrium caused by uterine curettage.
Objective:To explore the effect of different treatment time of 95% ethanol in rat uterine cavity on the endometrial injury.Methods:In this study,95% ethanol was injected into the rat uterine cavity for 15 s,30 s,60 sand 120 s,respectively.Seven days after the treatment,the rat uteri were collected,and the degree of endometrial repair,endometrial thickness and the number of gland were observed by morphological analysis.Results:It was shown that in the treatment group with 95% ethanol for 15 s,the number of gland in the endometrium was obviously reduced and fibrosis appeared in endometrium;In the treatment group with 95% ethanol for 30 s,the uterus showed the punctate congestions,the uterus surface was smooth,and the uterine cavity became smaller,with the area of fibrosis increased in the endometrium;Moreover,in the treatment groups with 95% ethanol for 60 sand 120 s,the uterus surface showed creases and obvious congestions,and the endometrium became much thinner.Conclusions:These results showed the status of endometrial injury in rat caused by 95% ethanol for 15～30 s,was similar to the morphological changes in human endometrium caused by uterine curettage.
引文
[1]牛婧雅,袁瑞.干细胞在子宫内膜损伤修复中的研究进展[J].现代妇产科进展,2014,23:920-922.
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[11]张璐,李莹,吕晓丹,等.大鼠子宫宫腔粘连模型的建立及评价指标[J].动物学杂志,2017,52:115-121.
[12] Khrouf M,Morel O,Hafiz A,et al.Evaluation of the rabbit as an experimental model for human uterine synechia[J].J Hum Reprod Sci,2012,5:175-180.
[13]倪冬冬,徐祥波,贺斌,等.Shh、Ptch1、Smo、Gli1在机械损伤大鼠子宫内膜的表达及意义[J].中国计划生育学杂志,2018,26:343-348.
[14] Wang X R,Wang C D,Liu X M,et al.Effect of PDGFRb antagonist imatinib on endometrial injury repairing in mouse model.[J].Asian Pac J Trop Med,2015,8:555-559.
[15]苏丽芬,洪宇.探讨刮宫术史对子宫内膜厚度的影响[J].中国实用医药,2017,12:79-81.
[2]张云.宫腔粘连证候证素特点分析及活血化瘀方的防治作用研究[D].北京中医药大学,2018.
[3] Conforti A,Alviggi C,Mollo A,et al.The management of Asherman syndrome:a review of literature[J].Reprod Biol Endocrinol,2013,11:118-128.
[4] Yu D,Wong YM,Cheong Y,et al.Asherman syndrome-one century later[J].Fertil Steril,2008,89:759-779.
[5]韩华,薛改,田菲,等.大鼠子宫内膜损伤模型的建立与评价[J].生殖与避孕,2017,37:33-38.
[6]曲军英,吕一帆,林茵,等.建立小鼠子宫内膜损伤模型的研究[J].福建医科大学学报,2011,45:34-36.
[7]王晏鹏,黄琼晓,徐盛,等.采用吸宫和电凝方法建立小鼠子宫内膜损伤模型的比较[J].浙江大学学报(医学版),2017,46,186-191.
[8] Li L,Shi J,Zhang QF,et al.Effect of curettage and copper wire on rabbit endometrium:a novel rabbit model of endometrial mechanical injury[J].Chin Med J,2011,124:1708-1713.
[9]张永裕,谭国胜,罗灿桥,等.多重损伤法建立大鼠宫腔粘连模型及其对子宫内膜LIF及整合素αvβ3的影响[J].中山大学学报(医学科学版),2016,37:15-22.
[10]许艳萍,赵桂峰,苗佳宁,等.体积分数95%乙醇建立薄型子宫模型大鼠子宫内膜中波蛋白和血管内皮生长因子的变化[J].中国组织工程研究,2016,20:718-722.
[11]张璐,李莹,吕晓丹,等.大鼠子宫宫腔粘连模型的建立及评价指标[J].动物学杂志,2017,52:115-121.
[12] Khrouf M,Morel O,Hafiz A,et al.Evaluation of the rabbit as an experimental model for human uterine synechia[J].J Hum Reprod Sci,2012,5:175-180.
[13]倪冬冬,徐祥波,贺斌,等.Shh、Ptch1、Smo、Gli1在机械损伤大鼠子宫内膜的表达及意义[J].中国计划生育学杂志,2018,26:343-348.
[14] Wang X R,Wang C D,Liu X M,et al.Effect of PDGFRb antagonist imatinib on endometrial injury repairing in mouse model.[J].Asian Pac J Trop Med,2015,8:555-559.
[15]苏丽芬,洪宇.探讨刮宫术史对子宫内膜厚度的影响[J].中国实用医药,2017,12:79-81.