HPLC法测定川贝秋梨膏清膏中款冬酮的含量

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英文篇名：Determination of Tussilagone in the Extract of Chuanbei Qiuli Cream by HPLC 作者：李玲慧 ; 温建英 ; 林玉桃 ; 林娟 英文作者：LI Ling-hui;WEN Jian-ying;LIN Yu-tao;LIN-Juan;Department of pharmacy of Fuzhou Traditional Chinese Medicine Hospital; 关键词：川贝秋梨膏 ; HPLC ; 清膏 ; 款冬酮 ; 含量测定 英文关键词：Chuanbei Qiuli cream;;HPLC;;Tussilagone;;Determination 中文刊名：HAIX 英文刊名：Strait Pharmaceutical Journal 机构：福州市中医院药学部; 出版日期：2019-03-15 出版单位：海峡药学 年：2019 期：v.31;No.230 基金：2017福州市卫生计生科技计划项目(项目编号:2017-S-wq34) 语种：中文; 页：HAIX201903017 页数：2 CN：03 ISSN：35-1173/R 分类号：53-54

摘要

目的建立川贝秋梨膏清膏中款冬酮的含量测定方法。方法采用高效液相色谱法,色谱柱:Ultimate XB-C_(18)(4.6mm×250mm,5μm),以乙腈-0.1%磷酸(85∶15)为流动相,流速为1.0mL·min~(-1);柱温为30℃;检测波长为220nm;进样量为20μL。结果款冬酮在2.39616～104μg·mL~(-1)范围内线性关系良好(r=0.9962),平均回收率为100.36%,RSD为1.43%(n=6)。结论该方法简便易行,可信度高,重现性好,为提高和完善该制剂的质量标准提供了依据。
        OBJECTIVE To establish a HPLC method for determination of tussilagonein the extract of Chuanbei Qiuli cream.METHODS The HPLC separation was achieved on a Ultimate XB-C_(18)(4.6 mm×250 mm,5μm) column.The mobile phase consisted of acetonitrile-0.1% phosphoric acid(85:15) at a flow rate of 1.0 mL·min~(-1),The detection wavelength was set at 220 nm and the column temperature was 30℃,injection volume was 20μL.RESULTS The linear range of tussilagone was 2.39616-104μg·mL~(-1)(r=0.9962).The average recovery rate was 100.36%,and RSD was 1.43%(n=6).CONCLUSION The method is simple,accurate,reproducibility and provide a basis for quality of the preparation of standards.

引文

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