中药中常见染色成分分析
|
摘要
近年来,市场上中药材(饮片)存在一定的染色问题,而且存在染色药材进入生产环节导致部分中成药含有染色剂的现象。中药中常见染色剂多为化工染料,对人体健康存在严重安全隐患,因此,国家药品监管部门相继批准了一系列补充检验方法作为控制这些问题产品认定的技术依据。由于这些染色成分不仅种类繁多,结构复杂,而且不断变化,因此,本文对其检测分析方法进行了总结,同时归纳了几种常见色系色素及其结构类似物的质谱与核磁等谱学信息,为准确定性此类成分以及相应补充检验方法的建立与实施提供科学依据。
In recent years,there exists the dying problem in Chinese material medica(prepared slices).Also raw materials with dyes are introduced into the manufacturing process.And it leads to the phenomenon of some Chinese patent medicine containing dyes.Most common dyes in traditional Chinese medicine are made by chemical synthesis.And there are serious safety risks to human health.As to these problematic products,national drug regulatory authorities have approved a series of supplementary test methods as the technical basis for judging.Due to the wide variety of the dyes with complex structures and changing constantly,this review summarizes the detection methods progress and spectral information including mass and nuclear magnetic resonance of several common dyes and their analogues.It provides scientific data for the accurate determination of these pigments and establishment and application of the corresponding supplementary detection methods.
In recent years,there exists the dying problem in Chinese material medica(prepared slices).Also raw materials with dyes are introduced into the manufacturing process.And it leads to the phenomenon of some Chinese patent medicine containing dyes.Most common dyes in traditional Chinese medicine are made by chemical synthesis.And there are serious safety risks to human health.As to these problematic products,national drug regulatory authorities have approved a series of supplementary test methods as the technical basis for judging.Due to the wide variety of the dyes with complex structures and changing constantly,this review summarizes the detection methods progress and spectral information including mass and nuclear magnetic resonance of several common dyes and their analogues.It provides scientific data for the accurate determination of these pigments and establishment and application of the corresponding supplementary detection methods.
引文
[1]魏锋,刘薇,严华,等.我国中药材及饮片的质量情况及有关问题分析[J].中国药学杂志,2015,50(4):277-283.
[2]符江,荆文光,章军,等.中药中非法添加问题研究现状与分析[J].中草药,2014,45(3):437-442.
[3]刘义梅,陈科力.当前中药材市场几种常见假劣药形式[J].中国中药杂志,2012,37(8):1089-1092.
[4]益磊,孟祥松,周志美,等.染色增重中药材及饮片科学监管技术方法探究[J].中国药事,2014,28(3):288-291.
[5]戴忠,鲁静,朱炯,等.关于中药国家评价性抽验的思考[J].中国药学杂志,2015,50(2):93-98.
[6]黄宝斌,许明哲,杨青云,等.中成药和中药材添加化学物质补充检验方法分析[J].药物分析杂志,2014,34(9):1701-1708.
[7]刘成伦,李小庆,王晶,等.偶氮类非食用色素的快速测定方法研究进展[J].食品科学,2009,30(5):273-276.
[8]饶伟文.中药染色掺假的研究进展[J].首都医药,2011,(8):16-18.
[9]马长宏,单作刚,齐家炜.中药染色掺假的研究进展[J].亚太传统医药,2011,7(2):153-154.
[10]薛虎寅,尹永梅,张太昌,等.偶氮类合成色素检测技术的研究进展[J].生物技术进展,2012,2(3):171-176.
[11]姜国萍,孙萍,朱日然.染色中药添加色素的检测技术研究进展[J].时珍国医国药,2015,26(3):694-696.
[12]闵春艳,付凌燕,汪祺,等.红花药材掺伪染色检测方法的实验研究[J].中国药事,2011,25(8):772-775.
[13]付凌燕,闵春艳,汪祺,等.市售西红花药材掺伪染色检测方法的实验研究[J].药物分析杂志,2012,32(1):74-77.
[14]胡青,孙健,张甦,等.中药中48种非法染色色素的TLC法检测[J].中国医药工业杂志,2015,46(7):695-700.
[15]金竹昳,陈旭,戴小峰,等.利用HPLC建立对比筛选法测定红花中色素金橙II[J].天然产物研究与开发,2014,26(3):384-387,402.
[16]胡佳,郭琳,何涛.27批红花柠檬黄、酸性红73、胭脂红染色及掺伪的检测[J].光明中医,2016,31(11):1561-1563.
[17]何轶,丁宁,王瑞忠,等.冠脉宁片中化工染料检查及血竭素高氯酸盐含量测定研究[J].中国药学杂志,2015,50(1):120-124.
[18]朱雪妍,谷立勍,何颂华,等.葛根芩连片中掺入染色剂的检测方法研究[J].药物分析杂志,2016,36(4):711-720.
[19]连云岚,杜鹃,乔玉峰.HPLC-MS法检测少腹逐瘀丸及原料药材中非法添加的7种黄色素[J].中草药,2016,47(18):3219-3223.
[20]陈安珍,李新荣,孙磊,等.UPLC-MS/MS法同时检测跌打丸中的6种非法染色物[J].药物分析杂志,2015,35(8):1453-1457.
[21]肖凌,侯俊杰,徐箐,等.红花中一种新掺伪染料的确认及6种染色成分检测方法研究[J].药物分析杂志,2014,34(7):1231-1236.
[22]何轶,雷银瓶,鲁静,等.HPLC-DAD-MS/MS法结合谱库筛查5种中药中21种合成染料[J].中成药,2016,38(12):2631-2635.
[23]胡青,孙健,于泓,等.快速液相-四级杆串联高分辨飞行时间质谱定性测定中药中50种染色色素[J].中国药学杂志,2016,51(15):1316-1323.
[24]钟建理,饶伟文,张治军.蒲黄近红外光谱法鉴别初探[J].药物鉴定,2009,18(4):32-33.
[25]钟建理,饶伟文,张治军,等.染色黄芩片的近红外光谱法鉴别初探[J].中国药师,2009,12(5):672-674.
[26]张玮玮,刘玲玲,武彦文,等.食用天然辣椒红色素中掺杂苏丹红的红外光谱快速识别[J].光谱学与光谱分析,2012,34(4):958-961.
[27]李丹,吕狄亚,陈辉,等.纸基-表面增强拉曼光谱法检测染色掺伪的红花药材[J].药物分析杂志,2015,35(8):1466-1470.
[28]李丹,汪冰,李晓,等.表面增强拉曼光谱法快速鉴别非法染色的关黄柏药材[J].药学研究,2015,34(4):202-205.
[29]郑滨兴,吕迪亚,方芳,等.基于整体柱的表面增强拉曼光谱法检测非法染色青黛[J].药学实践杂志,2015,33(5):426-428,459.
[30]焦建东,牟卫伟,权勤波,等.中药染色色素金胺O、日落黄和苋菜红的离子迁移谱技术快检方法研究[J].中国药学杂志,2016,51(6):483-487.
[31]常向彩.酸性红73间接ELISA检测方法的建立及初步应用[D].成都:西南民族大学硕士论文集,2013.
[32]Kusaka Takashi,Matsufuji Hiroshi,Chino Makoto,et al.Isolation,Identification and Determination of A Magenta Subsidiary Colour in Food Blue No.1(Brilliant Blue FCF)[J].Food Additivesand Contaminants,1999,16(12):501-507.
[33]Matsufuji Hiroshi,Kusaka Takashi,Tsukuda Masatoshi,et al.Structural Determination of Subsidiary Colors in Commercial Food Blue No.1(Brilliant blue FCF)product[J].J.Food Hyg.Soc.Japan,1998,39(1):7-12.
[34]Yamada Makiko,Kato Yoshiaki,Nakamura Mikio,et al.Structural Determination of Unknown SWubsidiary Colors in Commercial Food Red Nos.2 and 102[J].Chem Pharm.Bull,1998,46(3):494-499.
[35]Yamada Makiko,Nakamura Mikio,Yamada Takashi,et al.Structural Determination of Unknown Subsidiary Colors in Food Yellow No.5(Sunset Yellow FCF)[J].Chem.Pharm.Bull,1996,44(8):1624-1627.
[2]符江,荆文光,章军,等.中药中非法添加问题研究现状与分析[J].中草药,2014,45(3):437-442.
[3]刘义梅,陈科力.当前中药材市场几种常见假劣药形式[J].中国中药杂志,2012,37(8):1089-1092.
[4]益磊,孟祥松,周志美,等.染色增重中药材及饮片科学监管技术方法探究[J].中国药事,2014,28(3):288-291.
[5]戴忠,鲁静,朱炯,等.关于中药国家评价性抽验的思考[J].中国药学杂志,2015,50(2):93-98.
[6]黄宝斌,许明哲,杨青云,等.中成药和中药材添加化学物质补充检验方法分析[J].药物分析杂志,2014,34(9):1701-1708.
[7]刘成伦,李小庆,王晶,等.偶氮类非食用色素的快速测定方法研究进展[J].食品科学,2009,30(5):273-276.
[8]饶伟文.中药染色掺假的研究进展[J].首都医药,2011,(8):16-18.
[9]马长宏,单作刚,齐家炜.中药染色掺假的研究进展[J].亚太传统医药,2011,7(2):153-154.
[10]薛虎寅,尹永梅,张太昌,等.偶氮类合成色素检测技术的研究进展[J].生物技术进展,2012,2(3):171-176.
[11]姜国萍,孙萍,朱日然.染色中药添加色素的检测技术研究进展[J].时珍国医国药,2015,26(3):694-696.
[12]闵春艳,付凌燕,汪祺,等.红花药材掺伪染色检测方法的实验研究[J].中国药事,2011,25(8):772-775.
[13]付凌燕,闵春艳,汪祺,等.市售西红花药材掺伪染色检测方法的实验研究[J].药物分析杂志,2012,32(1):74-77.
[14]胡青,孙健,张甦,等.中药中48种非法染色色素的TLC法检测[J].中国医药工业杂志,2015,46(7):695-700.
[15]金竹昳,陈旭,戴小峰,等.利用HPLC建立对比筛选法测定红花中色素金橙II[J].天然产物研究与开发,2014,26(3):384-387,402.
[16]胡佳,郭琳,何涛.27批红花柠檬黄、酸性红73、胭脂红染色及掺伪的检测[J].光明中医,2016,31(11):1561-1563.
[17]何轶,丁宁,王瑞忠,等.冠脉宁片中化工染料检查及血竭素高氯酸盐含量测定研究[J].中国药学杂志,2015,50(1):120-124.
[18]朱雪妍,谷立勍,何颂华,等.葛根芩连片中掺入染色剂的检测方法研究[J].药物分析杂志,2016,36(4):711-720.
[19]连云岚,杜鹃,乔玉峰.HPLC-MS法检测少腹逐瘀丸及原料药材中非法添加的7种黄色素[J].中草药,2016,47(18):3219-3223.
[20]陈安珍,李新荣,孙磊,等.UPLC-MS/MS法同时检测跌打丸中的6种非法染色物[J].药物分析杂志,2015,35(8):1453-1457.
[21]肖凌,侯俊杰,徐箐,等.红花中一种新掺伪染料的确认及6种染色成分检测方法研究[J].药物分析杂志,2014,34(7):1231-1236.
[22]何轶,雷银瓶,鲁静,等.HPLC-DAD-MS/MS法结合谱库筛查5种中药中21种合成染料[J].中成药,2016,38(12):2631-2635.
[23]胡青,孙健,于泓,等.快速液相-四级杆串联高分辨飞行时间质谱定性测定中药中50种染色色素[J].中国药学杂志,2016,51(15):1316-1323.
[24]钟建理,饶伟文,张治军.蒲黄近红外光谱法鉴别初探[J].药物鉴定,2009,18(4):32-33.
[25]钟建理,饶伟文,张治军,等.染色黄芩片的近红外光谱法鉴别初探[J].中国药师,2009,12(5):672-674.
[26]张玮玮,刘玲玲,武彦文,等.食用天然辣椒红色素中掺杂苏丹红的红外光谱快速识别[J].光谱学与光谱分析,2012,34(4):958-961.
[27]李丹,吕狄亚,陈辉,等.纸基-表面增强拉曼光谱法检测染色掺伪的红花药材[J].药物分析杂志,2015,35(8):1466-1470.
[28]李丹,汪冰,李晓,等.表面增强拉曼光谱法快速鉴别非法染色的关黄柏药材[J].药学研究,2015,34(4):202-205.
[29]郑滨兴,吕迪亚,方芳,等.基于整体柱的表面增强拉曼光谱法检测非法染色青黛[J].药学实践杂志,2015,33(5):426-428,459.
[30]焦建东,牟卫伟,权勤波,等.中药染色色素金胺O、日落黄和苋菜红的离子迁移谱技术快检方法研究[J].中国药学杂志,2016,51(6):483-487.
[31]常向彩.酸性红73间接ELISA检测方法的建立及初步应用[D].成都:西南民族大学硕士论文集,2013.
[32]Kusaka Takashi,Matsufuji Hiroshi,Chino Makoto,et al.Isolation,Identification and Determination of A Magenta Subsidiary Colour in Food Blue No.1(Brilliant Blue FCF)[J].Food Additivesand Contaminants,1999,16(12):501-507.
[33]Matsufuji Hiroshi,Kusaka Takashi,Tsukuda Masatoshi,et al.Structural Determination of Subsidiary Colors in Commercial Food Blue No.1(Brilliant blue FCF)product[J].J.Food Hyg.Soc.Japan,1998,39(1):7-12.
[34]Yamada Makiko,Kato Yoshiaki,Nakamura Mikio,et al.Structural Determination of Unknown SWubsidiary Colors in Commercial Food Red Nos.2 and 102[J].Chem Pharm.Bull,1998,46(3):494-499.
[35]Yamada Makiko,Nakamura Mikio,Yamada Takashi,et al.Structural Determination of Unknown Subsidiary Colors in Food Yellow No.5(Sunset Yellow FCF)[J].Chem.Pharm.Bull,1996,44(8):1624-1627.