猴头菌深层发酵培养基筛选
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摘要
采用不同的碳氮源及不同比例的混合碳氮源对猴头菌进行深层发酵培养,以三个生物活性指标——生物量、多糖、腺苷为主要指标,筛选出最适菌丝体培养基组合。结果表明,最优培养基为(g/L):葡萄糖30、山药粉4、花生粉10、玉米浆干粉6、MgSO_4·7H_2O 0.5、KH_2PO_41、VB10.01。将组合进行三次重复验证,结果表明,菌丝体生物量为1.73g/100mL,多糖和腺苷含量分别为5.52%、0.247%。同时通过多组数据比较,将多糖和腺苷作为活性指标有一定可行性。
Different carbon and nitrogen sources and different proportions of mixed carbon and nitrogen sources were used for culture medium of Hericium erinaceus in deep Fermentation.Three bioactive indexes,biomass,polysaccharide and adenosine, were used as the main indexes to select the culture medium of mycelia. The results showed that the optimal medium was(g/L):glucose 30,yam powder 4,peanut powder 10,corn flour dry powder 6, MgSO_4·7 H_2 O 0.5, KH_2 PO_41, VB10.01. The culture medium was repeated three times. The results showed that the mycelium biomass was 1.73 g/100 mL, and the polysaccharide and adenosine content were 5.52% and 0.247%,respectively. At the same time,it is feasible to use polysaccharide and adenosine as active indexes through many groups of data.
Different carbon and nitrogen sources and different proportions of mixed carbon and nitrogen sources were used for culture medium of Hericium erinaceus in deep Fermentation.Three bioactive indexes,biomass,polysaccharide and adenosine, were used as the main indexes to select the culture medium of mycelia. The results showed that the optimal medium was(g/L):glucose 30,yam powder 4,peanut powder 10,corn flour dry powder 6, MgSO_4·7 H_2 O 0.5, KH_2 PO_41, VB10.01. The culture medium was repeated three times. The results showed that the mycelium biomass was 1.73 g/100 mL, and the polysaccharide and adenosine content were 5.52% and 0.247%,respectively. At the same time,it is feasible to use polysaccharide and adenosine as active indexes through many groups of data.
引文
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[14]刘艳芳,杨焱,贾薇,等.猴头菌深层发酵培养基筛选初探[J].食用菌学报,2003(3):26-31.
[2]张鹏,图力古尔,包海鹰.猴头菌属真菌化学成分及药理活性研究概述[J].菌物研究,2011(1):54-62.
[3]代玉山,韩铭,张金亭,等.小刺猴头菌丝体多糖的提纯及免疫与抗肿瘤活性的研究[J].中国临床研究,2011(10):871-873.
[4]张作法,吕国英,金群力,等.猴头菌液体发酵技术研究进展[J].食药用菌,2015(4):231-233.
[5]秦培鹏,傅力.猴头菌深层发酵工艺摇瓶培养条件优化的研究[J].农业开发与装备,2013(10):67-68.
[7]刘伟民,郭天龙,沈国栋,等.猴头菌液态发酵米糠工艺的优化[J].食品工业科技,2013(18):192-196.
[8]BYUNG KY,JUN BP,CHI HS.Hypolipidemic effect of an exo-biopolymer produced from a submerged mycelial cuhure of Hericium erinaaccum[J].Bioscience Biotechnology and Biochemistry,2003,67(6):245-258.
[9]KIM DM,PYUN CW.Isolation of antimicrobial suhstances from Hericium erinaceum[J].Mycobiology,2000(28):456-462.
[10]LEUN M,HSIU CL,SI FS.Autioxidant properties of several specialty mushrooms[J].Food Research Intemational,2002(35):153-160.
[11]刘晓明,闫云宇,毕华,等.猴头菇菌丝体和发酵液中多糖的含量测定[J].中国医药科学,2013(6):94-95.
[12]顾慧芬,庄意丽,张梦玲.HPLC法测定猴头菌片中腺苷[J].中成药,2012(7):1405-1406.
[13]杨宁,郝林.6种中药对猴头菌液体培养的影响[J].山西农业科学,2013,41(6):548-550.
[14]刘艳芳,杨焱,贾薇,等.猴头菌深层发酵培养基筛选初探[J].食用菌学报,2003(3):26-31.