基于FTIR的药桑主成分分析
|
摘要
目的利用红外光谱法结合主成分分析法建立药桑的指纹图谱,并对不同产地药桑的红外图谱进行模式识别。方法采用傅里叶红外光谱法(FTIR)绘制不同产地药桑果实及桑叶、桑枝的红外指纹谱图,应用主成分分析法进行模式识别分析。结果通过分析多个产地药桑以及桑叶、桑枝的红外指纹图谱,发现不同产地样品的红外光谱基本一致,有个别特征峰的相对吸收强度有所变化,指纹区小峰的微小差异,其主要化学物质在红外光谱吸收峰中的峰强和出峰位置上都很相似。结合主成分分析法,分布地不同会造成药桑在化学组成上存在着差异,即地理位置对药材质量是有影响的,只有采取对其复杂化学组分进行全面综合的评价方法,才能保障药桑产品质量的一致性,有效区分不同产地药桑。结论 FTIR法结合主成分分析可以区分不同地区的药桑果实及枝、叶,能为药桑的品质评价提供依据。
Objective To establish and compare the fingerprints of Morus nigra Linn from different producing areas by FTIR and by principal component analysis.Methods Infrared spectrometry and principal component analysis were used in this study.Results We analyzed the IR fingerprints of Morus nigra Linn from different areas and found that there were some differences in chemical component and the differences were caused by their geographical origins.The FTIR fingerprint and PCA analysis can be used to determine the consistency of the quality of Morus nigra Linn(mulberry)and their producing origins.Conclusion The FTIR fingerprint and PCA analysis could be used for separating and evaluating the quality of Morus nigra Linn(mulberry)from different producing areas.
Objective To establish and compare the fingerprints of Morus nigra Linn from different producing areas by FTIR and by principal component analysis.Methods Infrared spectrometry and principal component analysis were used in this study.Results We analyzed the IR fingerprints of Morus nigra Linn from different areas and found that there were some differences in chemical component and the differences were caused by their geographical origins.The FTIR fingerprint and PCA analysis can be used to determine the consistency of the quality of Morus nigra Linn(mulberry)and their producing origins.Conclusion The FTIR fingerprint and PCA analysis could be used for separating and evaluating the quality of Morus nigra Linn(mulberry)from different producing areas.
引文
[1]买买提依明.新疆药桑的研究[J].北方蚕业,2007,28(1):1-3.
[2]买买提依明,卢红,木合达尔,等.新疆药桑的有效成分测定及药理作用的研究初报[J].北方蚕业,2006,27(4):16-18.
[3]吴志平,周巧霞,顾振纶,等.桑树不同药用部位的降血糖效果比较[J].蚕业科学,2005,31(2):215-217.
[4]宋大可,买买提依明·乃比江.新疆药桑果汁化学成份及药物价值探讨[J].蚕桑通报,1994,25(4):23-34.
[5]国家药典委员会.中华人民共和国药典(一部)[M].北京:化学工业出版社,2005:211-212.
[6]赵明波,邓秀兰,王亚玲,等.红花RP-HPLC指纹图谱的建立及其质量研究[J].药学学报,2004,39(3):212-216.
[7]徐永群,孙素琴,袁子民,等.红外光谱结合主成分分析鉴别道地山药[J].分析化学,2002,30(10):1231-1233.
[8]徐永群,黄吴,周群,等.红外特征图谱和聚类分析法在赤芍产域分类鉴别种的应用[J].分析化学,2003,31(1):5-9.
[9]程存归,郭水良,陈宗良.FTIR直接测定法对川贝母和珠贝、小东贝的区别鉴定[J].中草药,2002,33(3):262-264.
[10]张志祥,刘鹏,康华靖,等.基于主成分分析和聚类分析的FTIR不同地理居群香果树多样性分化研究[J].光谱学与光谱分析,2008,28(9):2081-2085.
[2]买买提依明,卢红,木合达尔,等.新疆药桑的有效成分测定及药理作用的研究初报[J].北方蚕业,2006,27(4):16-18.
[3]吴志平,周巧霞,顾振纶,等.桑树不同药用部位的降血糖效果比较[J].蚕业科学,2005,31(2):215-217.
[4]宋大可,买买提依明·乃比江.新疆药桑果汁化学成份及药物价值探讨[J].蚕桑通报,1994,25(4):23-34.
[5]国家药典委员会.中华人民共和国药典(一部)[M].北京:化学工业出版社,2005:211-212.
[6]赵明波,邓秀兰,王亚玲,等.红花RP-HPLC指纹图谱的建立及其质量研究[J].药学学报,2004,39(3):212-216.
[7]徐永群,孙素琴,袁子民,等.红外光谱结合主成分分析鉴别道地山药[J].分析化学,2002,30(10):1231-1233.
[8]徐永群,黄吴,周群,等.红外特征图谱和聚类分析法在赤芍产域分类鉴别种的应用[J].分析化学,2003,31(1):5-9.
[9]程存归,郭水良,陈宗良.FTIR直接测定法对川贝母和珠贝、小东贝的区别鉴定[J].中草药,2002,33(3):262-264.
[10]张志祥,刘鹏,康华靖,等.基于主成分分析和聚类分析的FTIR不同地理居群香果树多样性分化研究[J].光谱学与光谱分析,2008,28(9):2081-2085.