康复新液缓解乙酸诱导大鼠急性溃疡性结肠炎及机制研究
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摘要
目的研究康复新液对乙酸诱导大鼠急性溃疡性结肠炎(ulcerative colitis,UC)细胞因子的影响,并初步探讨其作用机制。方法采用乙酸灌肠建立急性UC大鼠模型,除正常对照组及模型对照组外,其余各组分别灌肠给予结肠宁、康复新液2.50,1.25,0.625 g·kg-1,测定疾病活动指数(disease activity index,DAI)、结肠黏膜损伤指数(colon mucosa damage index,CMDI)及病理组织学评分(histopathological score,HS),采用酶联免疫吸附法检测大鼠血清中白介素-6(interleukin-6,IL-6)、C反应蛋白(C-reactive protein,CRP)、一氧化氮合酶(nitric oxide synthase,iNOS)以及结肠组织中表皮生长因子(epidermal growth factor,EGF)、环氧合酶-2(cyclooxygenase-2,COX-2)、髓过氧化物酶(myeloperoxidase,MPO)、iNOS的含量。结果与模型对照组相比,结肠宁及康复新液高剂量能显著降低UC大鼠IL-6、CRP、iNOS、COX-2、MPO表达,升高EGF的表达水平(P<0.01),改善组织病变情况。结论康复新液对乙酸诱导的UC有治疗作用,作用机制可能与下调大鼠体内炎症因子的表达量,上调EGF的表达量有关。
OBJECTIVE To study the effect of Kangfuxin on cytokines of acute ulcerative colitis(UC) induced by acetic acid in rats and explore its mechanism. METHODS The acute UC rat model was established by acetic acid enema. Except the normal control group and model control group, the other groups were gavaged respectively by Jiechangning, Kangfuxin 2.50,1.25, 0.625 g·kg-1. The disease activity index(DAI), colonic mucosa damage index(CMDI) and histopathological score(HS) were detected, interleukin-6(IL-6), C-reactive protein(CRP), nitric oxide synthase(iNOS) in serum and content of EGF, COX-2, MPO,iNOS in colon were determined by enzyme-linked immunosorbent method. RESULTS Compared with the model control group, Jiechangning and Kangfuxin with high dose could significantly reduce the expression level of IL-6, CRP, iNOS, COX-2,MPO and rise EGF in UC rats(P<0.01), and improve the pathological conditions of tissues. CONCLUSION Kangfuxin has therapeutic effect on acetic acid induced acute UC, and its mechanism may be related to down-regulating the expression of inflammatory factors and up-regulating the expression of EGF.
OBJECTIVE To study the effect of Kangfuxin on cytokines of acute ulcerative colitis(UC) induced by acetic acid in rats and explore its mechanism. METHODS The acute UC rat model was established by acetic acid enema. Except the normal control group and model control group, the other groups were gavaged respectively by Jiechangning, Kangfuxin 2.50,1.25, 0.625 g·kg-1. The disease activity index(DAI), colonic mucosa damage index(CMDI) and histopathological score(HS) were detected, interleukin-6(IL-6), C-reactive protein(CRP), nitric oxide synthase(iNOS) in serum and content of EGF, COX-2, MPO,iNOS in colon were determined by enzyme-linked immunosorbent method. RESULTS Compared with the model control group, Jiechangning and Kangfuxin with high dose could significantly reduce the expression level of IL-6, CRP, iNOS, COX-2,MPO and rise EGF in UC rats(P<0.01), and improve the pathological conditions of tissues. CONCLUSION Kangfuxin has therapeutic effect on acetic acid induced acute UC, and its mechanism may be related to down-regulating the expression of inflammatory factors and up-regulating the expression of EGF.
引文
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[12]GUAN L H,GONG Y F,ZHANG H,et al.Effect of Jiechangkang on MPO,NO and iNOS of colitis mice induced by oxazolone[J].Chin Tradit Pat Med(中成药),2013,35(4):669-673.
[13]ZHANG Z H,JIANG X,WANG J G.Role of cycloxygenase-2in ulcerative colitis[J].World Chin J Digestol(世界华人消化杂志),2008,16(31):3533-3538.
[14]HUANG Y,DONG L.Protective effect of purslane in a rat model of ulcerative colitis[J].China J Chin Mater Med(中国中药杂志),2011,36(19):2727-2730.
[15]OLIKONOMOU K A,KAPSORITAKIS A N,KAPSORITAKI A I,et al.Downregulation of serum epidermal growth factor in patients with inflammatory bowel disease.Is there a link with mucosal damage?[J].Growth Factors,2010,28(6):461-466.
[2]DING H,QIAN J M.Progress in the treatment of ulcerative colitis with 5-aminosalicylic acid[J].Eval Anal Drug-use Hosp China(中国医院用药评价与分析),2008(9):714-716.
[3]LIU R M.Clinical observation on 40 cases of ulcerative colitis treated by rehabilitation new liquid[J].Chin J Ethnomed Ethnopharm(中国民族民间医药),2013,22(14):64.
[4]ZHANG C L.Therapeutic observation and mechanism of Kangfuxin liquid in treating mouse model of ulcerative colitis[D].Beijing:Peking Union Medical College(北京协和医学院),2015.
[5]ZHANG H C,WANG P C,LIU H,et al.Effect of Kangfuxin on ulcerative colitis induced by trinitrobenzene sulfonic acid in rats[J].Chin Pharmacol Bull(中国药理学通报),2018,34(4):496-501.
[6]DU W W,LIU H,ZHANG H C,et al.Therapeutic effect and mechanism of Kangfuxin Liquid on oxazolone-induced ulcerative colitis in rats[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2017,23(4):126-131.
[7]ZHANG J,WEI Y K,LI Y E,et al.Effects and mechanism of Periplaneta americana extracted Ento-D on aceticacid-induced ulcerative colitis in rats[J].Chin J Tradit Chin Med(中华中医药杂志),2018,33(01):304-308.
[8]EKSTR?M G M.Oxazolone-induced colitis in rats:effects of budesonide,cyclosporin A,and 5-aminosalicylic acid[J].Scand J Gastroenterol,1998,33(2):174-179.
[9]WANG S X,PU J,LIU C Q,et al.Expression and clinical significance of cytokine TNF-α,IL-6 and IL-4 in ulcerative colitis[J].J Gastroenterol Hepatol(胃肠病学和肝病学杂志),2015,24(1):104-106.
[10]TURNER D,MACK D R,HYAMS J,et al.C-reactive protein(CRP),erythrocyte sedimentation rate(ESR)or both?Asystematic evaluation in pediatric ulcerative colitis[J].JCrohns Colitis,2011,5(5):423-429.
[11]LIU D,ZHOU L.The significance of platelet,C-reactive protein and erythrocyte sedimentation rate in patients with ulcerative colitis[J].Chin J Mod Drug Appl(中国现代药物应用),2015,9(18):13-15.
[12]GUAN L H,GONG Y F,ZHANG H,et al.Effect of Jiechangkang on MPO,NO and iNOS of colitis mice induced by oxazolone[J].Chin Tradit Pat Med(中成药),2013,35(4):669-673.
[13]ZHANG Z H,JIANG X,WANG J G.Role of cycloxygenase-2in ulcerative colitis[J].World Chin J Digestol(世界华人消化杂志),2008,16(31):3533-3538.
[14]HUANG Y,DONG L.Protective effect of purslane in a rat model of ulcerative colitis[J].China J Chin Mater Med(中国中药杂志),2011,36(19):2727-2730.
[15]OLIKONOMOU K A,KAPSORITAKIS A N,KAPSORITAKI A I,et al.Downregulation of serum epidermal growth factor in patients with inflammatory bowel disease.Is there a link with mucosal damage?[J].Growth Factors,2010,28(6):461-466.