栀子皮、仁、须中3个成分含量测定及指纹图谱
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摘要
目的:测定栀子不同部位(皮、仁、须)指标性成分含量及对其指纹图谱进行研究,比较栀子不同部位成分含量及组成差异,为栀子皮、仁药效差异提供理论依据;探究栀子须的开发利用价值,避免栀子药用资源浪费。方法:栀子苷、西红花苷Ⅰ含量测定采用HPLC法;总环烯迷萜苷含量测定采用紫外分光光度法,对栀子不同部位3个成分含量进行分析。采集栀子不同部位HPLC指纹图谱,分别建立不同产地不同加工方法栀子不同部位HPLC指纹图谱共有模式,并采用相似度评价系统软件进行数据分析;对栀子不同部位指纹图谱进行比对分析。结果:河南、福建、江西栀子皮、仁指标性成分的含量变化相同,栀子苷含量为福建>河南>江西,福建栀子皮中3种成分含量远远高于河南、江西。西红花苷Ⅰ和总环烯迷萜苷含量为福建>江西>河南,福建、江西总环烯迷萜苷含量远远高于河南。不同产地的栀子须中3个指标性成分含量比较,栀子苷、西红花苷I含量为福建>江西、河南;总环烯迷萜苷含量为福建>江西>河南。同一部位,栀子皮指纹图谱共标定22个共有峰,除了S13~S15外,其他样品的相似度在0.9以上;栀子仁指纹图谱共标定22个共有峰,除了S22~S30外,其他样品的相似度在0.9以上;栀子须指纹图谱共标定16个共有峰,除了S7~S9外,其他样品的相似度在0.9以上。不同部位,栀子须与皮、仁指纹图谱差别较大,须部位峰数减少,峰2,3,5的峰高须>皮>仁;仁部无峰X,峰高须>皮;除峰17外,仁部位图谱各峰的峰高均高于皮部位。结论:栀子皮、仁部位指标性成分含量存在显著差异;栀子须中总苷含量较高,可用于提取总环烯醚萜苷。所建指纹图谱可以反映栀子不同部位成分含量差异及种类分布,为栀子的药效物质基础研究提供理论支撑,为临床上栀皮、栀仁分开使用的科学性、合理性提供理论支持。
Objective:To determine the content of index components in different parts of Gardenia jasminoides(pericarp,seeds,whiskers),study the fingerprint,and compare the contents and compositions differences of different parts of G.jasminoides,in order to provide the theoretical basis for different efficacies of G.jasminoides pericarp and seeds,explore the exploitation and utilization values of G.jasminoides whiskers,and avoid waste of gardenia medicinal resources.Method:The contents of geniposide and crocetin Ⅰ was were determined by HPLC,the content of total iridoid glycosides was determined by ultraviolet spectrophotometry,and three index components in different parts of G.jasminoides were analyzed.HPLC fingerprints of different parts of G.jasminoides were collected,the common pattern of HPLC fingerprints of different parts of G.jasminoides of different origins and with different processing methods was established,and the similarity evaluation software was used for data analysis;comparative analysis on fingerprints of different parts of G.jasminoides was conducted.Result:Content change of index components in G.jasminoides pericarp and seeds from Henan,Fujian and Jiangxi were the same.Content of geniposide:Fujian > Henan > Jiangxi,the contents of three components in G.jasminoides pericarp from Fujian were much higher than those from Henan and Jiangxi,the contents of crocetinⅠ and total iridoid glycosides:Fujian > Jiangxi > Henan,the contents of total iridoid glycosides from Fujian,Jiangxi were much higher than those from Henan.The order of three index components in G.jasminoides whiskers from different origins from high to low,the content of geniposide and crocetin Ⅰ was Fujian > Jiangxi and Henan,the content of total iridoid glycosides was Fujian > Jiangxi > Henan.In the same part,there were 22 common peaks in the fingerprints of G.jasminoides pericarp,except for S13-S15,the similarity of other samples were more than 0.9;the fingerprints of G.jasminoides seeds had 22 common peaks,except for S22-S30,the similarities of other samples were more than 0.9;the fingerprints of G.jasminoides whiskers had 16 common peaks,except for S7-S9,the similarities of other samples were more than 0.9.In different parts,the fingerprints of G.jasminoides whiskers were significant different from those of pericarp and seeds,the number of peaks in G.jasminoides whiskers reduced,the order of height of peaks 2,3,5 of G.jasminoides from high to low were whiskers > gardenia> seeds.There was not peak X in the seeds,the height of peak X of gardenia in whiskers was higher than that in pericarp,except for the peak 17,the height of all peaks in seeds were higher than that in whiskers.Conclusion:There are significant differences in the contents of index components in G.jasminoides pericarp and seeds.The content of total glycosides in gardenia is high,suggesting that it can be used to extract total iridoid glycosides.The fingerprints can reflect the content difference and species distribution of different parts of G.jasminoides,so as to provide theoretical support for the studies for pharmacodynamic material basis of G.jasminoides and the scientificity and rationality of the separate application of G.jasminoides pericarp and seeds.
Objective:To determine the content of index components in different parts of Gardenia jasminoides(pericarp,seeds,whiskers),study the fingerprint,and compare the contents and compositions differences of different parts of G.jasminoides,in order to provide the theoretical basis for different efficacies of G.jasminoides pericarp and seeds,explore the exploitation and utilization values of G.jasminoides whiskers,and avoid waste of gardenia medicinal resources.Method:The contents of geniposide and crocetin Ⅰ was were determined by HPLC,the content of total iridoid glycosides was determined by ultraviolet spectrophotometry,and three index components in different parts of G.jasminoides were analyzed.HPLC fingerprints of different parts of G.jasminoides were collected,the common pattern of HPLC fingerprints of different parts of G.jasminoides of different origins and with different processing methods was established,and the similarity evaluation software was used for data analysis;comparative analysis on fingerprints of different parts of G.jasminoides was conducted.Result:Content change of index components in G.jasminoides pericarp and seeds from Henan,Fujian and Jiangxi were the same.Content of geniposide:Fujian > Henan > Jiangxi,the contents of three components in G.jasminoides pericarp from Fujian were much higher than those from Henan and Jiangxi,the contents of crocetinⅠ and total iridoid glycosides:Fujian > Jiangxi > Henan,the contents of total iridoid glycosides from Fujian,Jiangxi were much higher than those from Henan.The order of three index components in G.jasminoides whiskers from different origins from high to low,the content of geniposide and crocetin Ⅰ was Fujian > Jiangxi and Henan,the content of total iridoid glycosides was Fujian > Jiangxi > Henan.In the same part,there were 22 common peaks in the fingerprints of G.jasminoides pericarp,except for S13-S15,the similarity of other samples were more than 0.9;the fingerprints of G.jasminoides seeds had 22 common peaks,except for S22-S30,the similarities of other samples were more than 0.9;the fingerprints of G.jasminoides whiskers had 16 common peaks,except for S7-S9,the similarities of other samples were more than 0.9.In different parts,the fingerprints of G.jasminoides whiskers were significant different from those of pericarp and seeds,the number of peaks in G.jasminoides whiskers reduced,the order of height of peaks 2,3,5 of G.jasminoides from high to low were whiskers > gardenia> seeds.There was not peak X in the seeds,the height of peak X of gardenia in whiskers was higher than that in pericarp,except for the peak 17,the height of all peaks in seeds were higher than that in whiskers.Conclusion:There are significant differences in the contents of index components in G.jasminoides pericarp and seeds.The content of total glycosides in gardenia is high,suggesting that it can be used to extract total iridoid glycosides.The fingerprints can reflect the content difference and species distribution of different parts of G.jasminoides,so as to provide theoretical support for the studies for pharmacodynamic material basis of G.jasminoides and the scientificity and rationality of the separate application of G.jasminoides pericarp and seeds.
引文
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[5]王永香,米慧娟,李淼,等.不同产地栀子药材中8种主要药效成分的含量测定及聚类分析[J].中国实验方剂学杂志,2015,21(20):44-48.
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[14]刘怡,陈磊,张留记.不同产地栀子皮和栀子仁中有效成分的含量比较[J].中药新药与临床药理,2012,23(1):81-83.
[15]蔡仲希,傅祥平,龚千锋.栀子皮、栀子仁、全栀子中挥发油成分的比较研究[J].江西中医学院学报,2013,25(4):56-58.
[16]刘怡,陈磊,张留记.不同产地栀子皮和栀子仁中有效成分的含量比较[J].中药新药与临床药理,2012,23(1):81-83.
[17]刘燕玲.中药资源的缺失和浪费还要持续多久[J].中国药业,2007(18):15-16.
[18]杨相玉.栀子的指纹图谱与提取工艺研究[D].济南:山东大学,2009.
[19]刘怡,陈磊,张留记.不同产地栀子皮和栀子仁中有效成分的含量比较[J].中药新药与临床药理,2012,23(1):81-83.
[20]潘媛,王钰,张应,等.栀子种子性状变异及其与地理-气候因子的相关性研究[J].中药材,2017,40(9):2030-2035.
[2]孙娜,徐钢,赵远,等.HPLC测定栀子不同炮制品中栀子苷、绿原酸和西红花苷-Ⅰ含量[J].中国实验方剂学杂志,2014,20(19):50-53.
[3]肖日传,罗光明,董丽华,等.基于多波长HPLC-DAD比较栀子不同部位化学成分差异[J].中国中药杂志,2017,42(23):4636-4640.
[4]蔡妙婷,左月明,张忠立,等.栀子化学成分(Ⅳ)[J].中国实验方剂学杂志,2014,20(22):88-91.
[5]王永香,米慧娟,李淼,等.不同产地栀子药材中8种主要药效成分的含量测定及聚类分析[J].中国实验方剂学杂志,2015,21(20):44-48.
[6]肖日传,苗琦,罗光明,等.栀子药材商品规格等级标准研究[J].中国中药杂志,2017,42(4):663-668.
[7]孙思邈.备急千金要方校释[M].北京:人民卫生出版社,1998:125,160,477.
[8]太平惠民和剂局.太平惠民和剂局方[M].北京:人民卫生出版社,1985:271,430.
[9]龚廷贤.寿世保元[M].上海:上海科学技术出版社,1989:102.
[10]杨时泰.本草述钩元[M].上海:上海科学技术出版社,1958:522.
[11]王孝涛.历代中药炮制法汇典(古代部分)[M].南昌:江西科学技术出版社,1986:330.
[12]汪昂.本草备要[M].天津:天津科学技术出版社,1993:129.
[13]李梃.医学入门[M].天津:天津科学技术出版社,1999:328.
[14]刘怡,陈磊,张留记.不同产地栀子皮和栀子仁中有效成分的含量比较[J].中药新药与临床药理,2012,23(1):81-83.
[15]蔡仲希,傅祥平,龚千锋.栀子皮、栀子仁、全栀子中挥发油成分的比较研究[J].江西中医学院学报,2013,25(4):56-58.
[16]刘怡,陈磊,张留记.不同产地栀子皮和栀子仁中有效成分的含量比较[J].中药新药与临床药理,2012,23(1):81-83.
[17]刘燕玲.中药资源的缺失和浪费还要持续多久[J].中国药业,2007(18):15-16.
[18]杨相玉.栀子的指纹图谱与提取工艺研究[D].济南:山东大学,2009.
[19]刘怡,陈磊,张留记.不同产地栀子皮和栀子仁中有效成分的含量比较[J].中药新药与临床药理,2012,23(1):81-83.
[20]潘媛,王钰,张应,等.栀子种子性状变异及其与地理-气候因子的相关性研究[J].中药材,2017,40(9):2030-2035.