鸭3型腺病毒的分离鉴定及其fiber基因分析
|
摘要
为确诊引起福建某番鸭场以肝脏肿大出血为特征的病原,采用病毒分离、PCR检测、动物回归试验、电镜观察、组织病理学诊断、fiber基因序列测定与分析等方法进行研究。结果显示:所采样品盲传15代后获得病毒,PCR可以扩增出鸭3型腺病毒(DAdV-3)的特异性片段,分离病毒可引起3日龄番鸭发病死亡,死亡率为28.6%。人工感染死亡鸭的肝脏超薄切片呈现大量晶格状排列的典型禽腺病毒颗粒,肝脏的组织病理学以"包涵体肝炎"病变为特征。其fiber基因与已报道的DAdV-3毒株核苷酸同源性均为100%。以上结果证实分离的病原为DAdV-3。本试验为DAdV-3的诊断提供了科学依据。
引文
[1]周镇海.鸭腺病毒3型基因组序列分析及致病性研究[D].广州:华南农业大学,2016.
[2]冯柳柳.减蛋综合征病毒对种鸭的致病性研究[D].泰安:山东农业大学,2015.
[3] BOUQUET J F, MOREAU Y, MCFERRAN J B, et al. Isolation and characterisation of an adenovirus isolated from Muscovy ducks[J]. Avian Pathology, 1982, 11(2):301-307.
[4] MAREK A, KAJAN G L, KOSIOL C, et al. Complete genome sequences of pigeon adenovirus 1 and duck adenovirus 2 extend the number of species within the genus Aviadenovirus[J]. Virology, 2014, 462-463:107-114.
[5]张新珩,蔺文成,周振海,等.新型鸭Ⅲ型腺病毒的分离鉴定及遗传进化分析[J].中国兽医学报,2017,37(1):29-32.
[6] ZHANG X H, ZHONG Y J, ZHOU Z H, et al. Molecular characterization, phylogeny analysis and pathogenicity of a Muscovy duck adenovirus strain isolated in China in2014[J]. Virology, 2016(493):12-21.
[7]中华人民共和国农业行业标准.禽流感病毒RT-PCR检测方法:NY/T 772-2013[S].北京:中国农业出版社,2014.
[8]施少华,程龙飞,江斌,等.韩国型鸭肝炎病毒福建株的分离与鉴定[J].畜牧与兽医,2010,42(12):9-12.
[9]刘家林,姜骞,司昌德,等.番鸭细小病毒与鹅细小病毒PCR鉴别诊断方法的建立[J].中国兽医科学,2007,37(6):469-472.
[10]胡奇林,林锋强,陈少莺,等.应用RT-PCR技术检测番鸭呼肠孤病毒[J].中国兽医学报,2004,24(3):231-232.
[11]陈峰,招丽婵,覃健萍,等.应用宏基因组学鉴定国内第一株鸭2型腺病毒[J].中国预防兽医学报,2015,37(12):903-907.
[12]陈亮,黄路生,刘晓文,等.鸭2型腺病毒的分离鉴定及hexon基因的序列分析[J].中国兽医科学,2016,46(12):1538-1542.
[13]陈仕龙,俞博,肖世峰,等.鸭2型腺病毒感染导致番鸭“肝白化病”的研究[J].福建农业科技,2017(8):1-3.
[14] SCHACHNER A, MARKER A, JASKULSKA B, et al. Recombinant FAdv-4 fiber-2 protein protects chickens against hepatitis-hydropericardium syndrome(HHS)[J]. Vaccine, 2014, 32(9):1086-1092.
[15] WANG P, ZHANG J J, WANG W K, et al. A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2[J]. Vet Res, 2018, 49(1):29.
[16] CHEN L, YIN L J, ZHOU Q F, et al. Immunogenicity and protective efficacy of recombinant fiber-2 protein in protecting SPF chickens against fowl adenovirus 4[J]. Vaccine, 2018, 36(9):1203-1208.
[2]冯柳柳.减蛋综合征病毒对种鸭的致病性研究[D].泰安:山东农业大学,2015.
[3] BOUQUET J F, MOREAU Y, MCFERRAN J B, et al. Isolation and characterisation of an adenovirus isolated from Muscovy ducks[J]. Avian Pathology, 1982, 11(2):301-307.
[4] MAREK A, KAJAN G L, KOSIOL C, et al. Complete genome sequences of pigeon adenovirus 1 and duck adenovirus 2 extend the number of species within the genus Aviadenovirus[J]. Virology, 2014, 462-463:107-114.
[5]张新珩,蔺文成,周振海,等.新型鸭Ⅲ型腺病毒的分离鉴定及遗传进化分析[J].中国兽医学报,2017,37(1):29-32.
[6] ZHANG X H, ZHONG Y J, ZHOU Z H, et al. Molecular characterization, phylogeny analysis and pathogenicity of a Muscovy duck adenovirus strain isolated in China in2014[J]. Virology, 2016(493):12-21.
[7]中华人民共和国农业行业标准.禽流感病毒RT-PCR检测方法:NY/T 772-2013[S].北京:中国农业出版社,2014.
[8]施少华,程龙飞,江斌,等.韩国型鸭肝炎病毒福建株的分离与鉴定[J].畜牧与兽医,2010,42(12):9-12.
[9]刘家林,姜骞,司昌德,等.番鸭细小病毒与鹅细小病毒PCR鉴别诊断方法的建立[J].中国兽医科学,2007,37(6):469-472.
[10]胡奇林,林锋强,陈少莺,等.应用RT-PCR技术检测番鸭呼肠孤病毒[J].中国兽医学报,2004,24(3):231-232.
[11]陈峰,招丽婵,覃健萍,等.应用宏基因组学鉴定国内第一株鸭2型腺病毒[J].中国预防兽医学报,2015,37(12):903-907.
[12]陈亮,黄路生,刘晓文,等.鸭2型腺病毒的分离鉴定及hexon基因的序列分析[J].中国兽医科学,2016,46(12):1538-1542.
[13]陈仕龙,俞博,肖世峰,等.鸭2型腺病毒感染导致番鸭“肝白化病”的研究[J].福建农业科技,2017(8):1-3.
[14] SCHACHNER A, MARKER A, JASKULSKA B, et al. Recombinant FAdv-4 fiber-2 protein protects chickens against hepatitis-hydropericardium syndrome(HHS)[J]. Vaccine, 2014, 32(9):1086-1092.
[15] WANG P, ZHANG J J, WANG W K, et al. A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2[J]. Vet Res, 2018, 49(1):29.
[16] CHEN L, YIN L J, ZHOU Q F, et al. Immunogenicity and protective efficacy of recombinant fiber-2 protein in protecting SPF chickens against fowl adenovirus 4[J]. Vaccine, 2018, 36(9):1203-1208.